JP2724130B2 - Chemical solution supply method and device for culture tank - Google Patents
Chemical solution supply method and device for culture tankInfo
- Publication number
- JP2724130B2 JP2724130B2 JP8003593A JP359396A JP2724130B2 JP 2724130 B2 JP2724130 B2 JP 2724130B2 JP 8003593 A JP8003593 A JP 8003593A JP 359396 A JP359396 A JP 359396A JP 2724130 B2 JP2724130 B2 JP 2724130B2
- Authority
- JP
- Japan
- Prior art keywords
- chemical
- culture tank
- chemical solution
- pot
- line
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】本発明は培養槽内の薬液供給
方法及び装置に係り、特に、培養運転時、酸，アルカ
リ，消泡剤等の薬液を多量に消費する培養系に好適な培
養槽用薬液供給方法及び装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and an apparatus for supplying a chemical solution in a culture tank, and more particularly, to a culture tank suitable for a culture system which consumes a large amount of a chemical such as an acid, an alkali, and a defoamer during a culture operation. The present invention relates to a method and an apparatus for supplying a chemical solution.
【０００２】[0002]
【従来の技術】従来、培養槽の酸，アルカリ，消泡剤等
の薬液が培養中に不足した場合は、薬液を個別にオート
クレーブで殺菌し、火炎投入による方法で供給してい
た。また、供給物は異なるが培養槽に無菌的に種菌を供
給（植菌）する場合は、火炎植菌方法で行なわれてい
た。なお、この種の装置として関連するものには例えば
特開昭６０−１１０２８１号公報が挙げられる。2. Description of the Related Art Conventionally, when a chemical solution such as an acid, an alkali, and a defoamer in a culture tank becomes insufficient during culturing, the chemical solution is individually sterilized in an autoclave and supplied by a method of introducing a flame. In addition, when a seed is supplied (inoculated) to a culture tank aseptically, a flame inoculation method has been used, although the supply is different. Japanese Patent Application Laid-Open No. 60-110281 is an example of this type of apparatus.
【０００３】[0003]
【発明が解決しようとする課題】上記従来技術は薬液の
供給量が多量に及び培養系について配慮がされておら
ず、薬液ポットへの薬液供給時の取扱いが前記の如く火
炎投入によるもので、その操作が大変めんどうであり、
外気中の雑菌が混入するという課題があった。In the prior art described above, the supply amount of the chemical solution is large and no consideration is given to the culture system, and the handling at the time of supplying the chemical solution to the chemical solution pot is performed by injecting a flame as described above. The operation is very troublesome,
There is a problem that various bacteria in the outside air are mixed.
【０００４】本発明の目的は薬液ポットへの薬液供給時
の操作性を向上させ、無菌性を向上できる培養槽用薬液
供給方法及び装置を提供することにある。An object of the present invention is to provide a method and an apparatus for supplying a chemical solution for a culture tank, which can improve operability when supplying a chemical solution to a chemical solution pot and improve sterility.
【０００５】[0005]
【課題を解決するための手段】上記目的は、培養槽と、
該培養槽へ酸，アルカリ，消泡剤等の薬液を供給するジ
ャケット付薬液ポットとに薬液供給ラインと、均圧ライ
ンを経由する空気供給ラインとをおのおの並列に自動弁
を介して構成し、自動弁を用いたスチームラインと冷却
ラインとでシャケット付薬液ポットの温度制御を行な
い、ジャケット付薬液ポットへの薬液供給を前記各ライ
ンに設けた自動弁を介してシーケンサーで制御すること
により、達成される。The object of the present invention is to provide a culture tank,
A chemical solution pot with a jacket for supplying a chemical solution such as an acid, an alkali or an antifoaming agent to the culture tank, a chemical solution supply line, and an air supply line passing through a pressure equalizing line, each being configured in parallel with an automatic valve; Achieved by controlling the temperature of the chemical solution pot with a shacket in the steam line and the cooling line using an automatic valve, and controlling the supply of the chemical solution to the chemical solution pot with a jacket by a sequencer via the automatic valves provided in the respective lines. Is done.
【０００６】ジャケット付薬液ポットと培養槽との各ラ
インは総て自動弁を介して連絡する流路としているの
で、前記各自動弁をシーケンサーにより制御し、培養運
転中の薬液供給時、培養槽本体とジャケット付薬液ポッ
トとの流路をしゃ断する。従って、各ラインの無菌性が
確保でき、自動殺菌するスチームラインを働らかせるこ
とによりさらに無菌性が向上する。また、ジャケット付
薬液ポットの温度管理をスチームラインと冷却ライン
と、温度センサーを備えた温度信号変換器とによりシー
ケンサーで制御できる。さらに、上記各操作を総てシー
ケンサー制御できるので操作性も向上できる。[0006] Since each line between the jacketed chemical solution pot and the culture tank is a flow path communicating with each other through an automatic valve, each of the automatic valves is controlled by a sequencer to supply the chemical solution during the culture operation. Cut off the flow path between the main unit and the jacketed chemical pot. Therefore, the sterility of each line can be ensured, and the sterility is further improved by operating the steam line for automatic sterilization. The temperature of the jacketed chemical pot can be controlled by a sequencer using a steam line, a cooling line, and a temperature signal converter having a temperature sensor. Furthermore, since all of the above operations can be controlled by the sequencer, the operability can be improved.
【０００７】[0007]
【発明の実施の形態】以下、本発明の一実施例を図１に
より説明する。図において、１はジャケット付薬液ポッ
ト、２は培養槽、３はエアーフィルター、４はシーケン
サーである。ジャケット付薬液ポット１はその上部から
出る均圧ライン７’と均圧ライン仕切弁８（自動弁）、
均圧ライン７、自動弁２１、空気供給ライン２３とを介
して培養槽２に流れる流路を設けている。均圧ライン
７、７’は薬液ポット１内の圧力と培養槽２内の圧力と
を等しくして、薬液供給量の制御を極微量の範囲まで可
能とするために設けられている。また、ジャケット付薬
液ポット１の下部から出る薬液供給ライン２４は、自動
弁１４、１６を介して培養槽２に流れる流路を設けてい
る。さらに、ジャケット付薬液ポット１の上部には自動
弁１０を用いた排気ライン２５を設けている。さらに、
ジャケット付薬液ポット１の温度調整用のためのスチー
ムライン２６と、冷却ライン２７、２７’と殺菌ライン
２８、２９とをそれぞれ自動弁１３、１１、１２、１
５、９を用いて設けている。３０、３１は排水ラインで
それぞれ自動弁１７、１８を用いて設けている。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described below with reference to FIG. In the figure, 1 is a chemical pot with a jacket, 2 is a culture tank, 3 is an air filter, and 4 is a sequencer. The jacketed chemical solution pot 1 has a pressure equalizing line 7 'coming out from the upper part thereof, a pressure equalizing line gate valve 8 (automatic valve),
A flow path that flows to the culture tank 2 via the pressure equalizing line 7, the automatic valve 21, and the air supply line 23 is provided. The equalizing lines 7 and 7 'are provided to equalize the pressure in the chemical solution pot 1 and the pressure in the culture tank 2 so that the supply of the chemical solution can be controlled to an extremely small range. In addition, a drug solution supply line 24 that exits from the lower portion of the jacketed drug solution pot 1 has a flow path that flows to the culture tank 2 via the automatic valves 14 and 16. Further, an exhaust line 25 using the automatic valve 10 is provided above the jacketed chemical solution pot 1. further,
A steam line 26 for temperature adjustment of the jacketed chemical liquid pot 1, cooling lines 27 and 27 ′ and sterilizing lines 28 and 29 are respectively connected to automatic valves 13, 11, 12 and 1.
5 and 9 are provided. Reference numerals 30 and 31 denote drain lines provided with automatic valves 17 and 18, respectively.
【０００８】ジャケット付薬液ポット１の温度制御のた
めに、該ジャケット付薬液ポット１内に温度センサー６
を設け、温度信号変換器５、信号線３２を介しシーケン
サー４に連絡している。また、無菌エアーを培養槽２へ
供給するため、エアーフィルター３（除菌フィルター）
を介して均圧ライン７に連絡している。To control the temperature of the jacketed chemical solution pot 1, a temperature sensor 6 is provided in the jacketed chemical solution pot 1.
Is connected to the sequencer 4 via the temperature signal converter 5 and the signal line 32. Air filter 3 (sterilization filter) to supply sterile air to culture tank 2
To the pressure equalizing line 7 via
【０００９】以上の構成において、培養槽２運転時は自
動弁８、２１は開状態とし、培養槽２への通気および薬
液を投入（供給）するため、均圧ライン７、７’と空気
供給ライン２３とに加圧用エアーを供給している。自動
弁１６は培養槽２内のＰＨ、泡面等の変動により、自動
的に弁開度を制御される。In the above configuration, when the culture tank 2 is operated, the automatic valves 8 and 21 are opened, and in order to ventilate the culture tank 2 and to supply (supply) a chemical solution, the pressure equalizing lines 7 and 7 ′ and the air supply are provided. Air for pressurization is supplied to the line 23. The valve opening of the automatic valve 16 is automatically controlled by fluctuations in PH, bubble level, and the like in the culture tank 2.
【００１０】培養中にジャケット付薬液ポット１中の薬
液がなくなり、新たに薬液をジャケット付薬液ポット１
に追加し殺菌を行なう場合、殺菌準備モードを選択（押
ボタン式）するとシーケンサー４により自動弁８、自動
弁１６とが強制的に閉となり、培養槽２系との流路がた
たれる。During the culture, the drug solution in the jacketed drug pot 1 is exhausted, and a new drug solution is newly added to the jacketed drug solution pot 1.
When the sterilization preparation mode is selected (push-button type), the automatic valve 8 and the automatic valve 16 are forcibly closed by the sequencer 4 and the flow path with the culture tank 2 system is drained.
【００１１】この時、ジャケット付薬液ポット１の排気
ライン２５の自動弁１０が開となりジャケット付薬液ポ
ット１の内圧は開放され、薬液供給（投入）が可能とな
る。At this time, the automatic valve 10 of the exhaust line 25 of the jacketed chemical pot 1 is opened, the internal pressure of the jacketed chemical pot 1 is released, and the chemical solution can be supplied (charged).
【００１２】薬液供給（投入）後、殺菌モードを選択
（押ボタン式）すると、スチームライン２８、２８’、
２９自動弁９、１４、１５、２０および、排水ライン３
０、３１自動弁１７、１８、排気ライン２５自動弁１０
とがシーケンサー４に従い作動し、殺菌を開始し、温度
信号変換器５と、温度センサー６とにより殺菌温度の制
御を行なう。When the sterilization mode is selected (push-button type) after supplying (feeding) the chemical, the steam lines 28, 28 ',
29 automatic valves 9, 14, 15, 20 and drain line 3
0, 31 automatic valves 17, 18; exhaust line 25 automatic valves 10
Operate according to the sequencer 4 to start sterilization, and the temperature signal converter 5 and the temperature sensor 6 control the sterilization temperature.
【００１３】殺菌後は、冷却ライン２７、２７’の自動
弁１１、１２が作動し冷却を行ない、所定の温度まで下
げたところで殺菌が終了する。After sterilization, the automatic valves 11 and 12 of the cooling lines 27 and 27 'are operated to perform cooling, and the sterilization is completed when the temperature is lowered to a predetermined temperature.
【００１４】次にストップボタンを押すことにより均圧
ライン７’の自動弁８が開となり、自動弁（薬液投入
弁）１６が作動を開始し、通常の培養運転状態に戻る
（上記選択モードボタンはシーケンサー制御盤に組込ん
でいる。）。Next, when the stop button is pressed, the automatic valve 8 of the pressure equalizing line 7 'is opened, the automatic valve (chemical solution input valve) 16 starts to operate, and returns to the normal culture operation state (the above selection mode button). Is built into the sequencer control panel.)
【００１５】なお、本実施例のジャケット付薬液ポット
の代わりに缶内コイルを用いた温度制御手段を有する薬
液ポットの構成としても良い。さらに、本実施例のシー
ケンサーの代わりにマイクロコンピューターを用いて構
成しても良い。It is to be noted that a chemical pot having temperature control means using an in-can coil may be used instead of the jacketed chemical pot of the present embodiment. Further, a microcomputer may be used instead of the sequencer of this embodiment.
【００１６】本実施例によれば、薬液供給（投入）の操
作性の向上および無菌性が向上できる効果がある。特
に、殺菌モードにおいては、薬液ポット１の殺菌に加え
て、仕切弁（自動弁）８から薬液ポット１側の均圧ライ
ン７’及び自動弁１６から薬液ポット１側の薬液供給ラ
イン２４も殺菌されるので、薬液投入にともない汚染の
可能性がある全ての系が殺菌され、完全な無菌性を確保
できる。According to this embodiment, there is an effect that the operability of supplying (feeding) the chemical solution and the sterility can be improved. In particular, in the sterilization mode, in addition to the sterilization of the chemical pot 1, the equalizing line 7 'from the gate valve (automatic valve) 8 to the chemical pot 1 and the chemical supply line 24 from the automatic valve 16 to the chemical pot 1 are also sterilized. Therefore, all the systems that may be contaminated with the introduction of the chemical solution are sterilized, and complete sterility can be ensured.
【００１７】[0017]
【発明の効果】本発明によれば、培養運転中に、ジャケ
ット付薬液ポットへの薬液供給（追加投入）ができ、か
つ、自動殺菌操作を行なうことができる。According to the present invention, it is possible to supply (additionally) a chemical solution to a jacketed chemical solution pot and to perform an automatic sterilization operation during the culture operation.
【００１８】従って、薬液をあらかじめ個別にオートク
レープにて殺菌を行ない、火炎投入にてポットに供給す
る必要がなくなるので、薬液供給時の操作性が向上で
き、無菌性の確保・向上が図れる効果がある。Therefore, it is not necessary to sterilize the chemicals individually in advance in an autoclave and supply them to the pot by flame injection, so that the operability in supplying the chemicals can be improved and the sterility can be secured and improved. There is.
【図１】本発明の一実施例の培養槽用薬液供給装置の概
略構成図である。FIG. 1 is a schematic configuration diagram of a culture solution supply device for a culture tank according to one embodiment of the present invention.
１…ジャケット付薬液ポット、４…シーケンサー、５…
温度信号変換器、６…温度センサー、８〜１８…自動
弁。1 ... chemical pot with jacket, 4 ... sequencer, 5 ...
Temperature signal converter, 6 temperature sensor, 8-18 automatic valve.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 藤本 正勝 山口県下松市大字東豊井794番地 株式 会社 日立製作所 笠戸工場内 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Masakatsu Fujimoto 794, Higashi-Toyoi, Kazamatsu-shi, Yamaguchi Prefecture Hitachi, Ltd. Kasado Plant
Claims (2)
る薬液供給ポットからなる培養装置において、上記培養
槽と上記薬液供給ポットとの間に、上記培養槽の圧力と
上記薬液供給ポットの圧力とを等しくする均圧ラインと
薬液が流れる薬液供給ラインとを設け、各ラインの途中
にそれぞれ自動弁を設け、上記薬液供給ポットを殺菌す
る際には、上記各自動弁より上記薬液供給ポット側の各
ライン部分をスチーム殺菌することを特徴とする培養槽
用薬液供給方法。1. A culture apparatus comprising a culture tank and a chemical supply pot for supplying a chemical to the culture tank, wherein a pressure of the culture tank and the chemical supply pot are provided between the culture tank and the chemical supply pot. A pressure equalizing line for equalizing the pressure and a chemical liquid supply line through which the chemical liquid flows are provided. An automatic valve is provided in the middle of each line, and when sterilizing the chemical liquid supply pot, the chemical liquid supply from each of the automatic valves is performed. A method for supplying a chemical solution for a culture tank, wherein each line portion on the pot side is steam-sterilized.
する薬液供給ポットと、第１の自動弁を有し、上記培養
槽と上記薬液供給ポットとの間に設けられ、上記培養槽
及び薬液供給ポットの圧力を等しくする均圧ラインと、
第２の自動弁を有し、上記培養槽と上記薬液供給ポット
との間に設けられ、上記薬液が流れる薬液供給ライン
と、上記第１及び第２の自動弁を閉じた状態で、上記薬
液供給ポットを含む上記第１及び第２の自動弁間の流路
をスチーム殺菌するスチーム殺菌手段とからなることを
特徴とする培養槽用薬液供給装置。2. A culture tank, a chemical supply pot for supplying a chemical to the culture tank, and a first automatic valve, wherein the first automatic valve is provided between the culture tank and the chemical supply pot. An equalizing line for equalizing the pressures of the tank and the chemical supply pot,
A chemical solution supply line provided between the culture tank and the chemical solution supply pot, the chemical solution supply line through which the chemical solution flows, and the chemical solution with the first and second automatic valves closed; And a steam sterilizing means for steam sterilizing a flow path between the first and second automatic valves including a supply pot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8003593A JP2724130B2 (en) | 1996-01-12 | 1996-01-12 | Chemical solution supply method and device for culture tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8003593A JP2724130B2 (en) | 1996-01-12 | 1996-01-12 | Chemical solution supply method and device for culture tank |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63074343A Division JPH074223B2 (en) | 1988-03-30 | 1988-03-30 | Method and device for supplying chemical liquid for culture tank |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH08228757A JPH08228757A (en) | 1996-09-10 |
| JP2724130B2 true JP2724130B2 (en) | 1998-03-09 |
Family
ID=11561776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8003593A Expired - Lifetime JP2724130B2 (en) | 1996-01-12 | 1996-01-12 | Chemical solution supply method and device for culture tank |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2724130B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6378957B2 (en) * | 2014-07-16 | 2018-08-22 | 水ｉｎｇ株式会社 | Defoamer charging device and defoamer charging method |
| JP6420662B2 (en) * | 2014-12-26 | 2018-11-07 | 川崎重工業株式会社 | Chemical supply apparatus and method |
| CN107574119A (en) * | 2017-09-28 | 2018-01-12 | 上海国强生化工程装备有限公司 | A kind of parallel filling device |
| CN108330066A (en) * | 2018-04-11 | 2018-07-27 | 江苏辉强新能源科技有限公司 | A kind of integrated intelligent temperature control steaming aerated koji making machine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5847485A (en) * | 1981-08-18 | 1983-03-19 | デ−・エル・エム・ドクトル・ミユラ−・アクチエンゲゼルシャフト | Method and apparatus for culturing microorganism |
| JPS62289170A (en) * | 1986-06-10 | 1987-12-16 | Teijin Ltd | Method of cell culture and device therefor |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60104100U (en) * | 1983-12-21 | 1985-07-16 | 株式会社日立製作所 | system culture tank |
-
1996
- 1996-01-12 JP JP8003593A patent/JP2724130B2/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5847485A (en) * | 1981-08-18 | 1983-03-19 | デ−・エル・エム・ドクトル・ミユラ−・アクチエンゲゼルシャフト | Method and apparatus for culturing microorganism |
| JPS62289170A (en) * | 1986-06-10 | 1987-12-16 | Teijin Ltd | Method of cell culture and device therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08228757A (en) | 1996-09-10 |
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