JP3698755B2 - Target screen - Google Patents

Description

[0001]
[Industrial application fields]
The present invention relates to a target SCREEN used in filament centering adjustment of the microscope light source.
[0002]
[Prior art]
Conventionally, when starting to use a microscope or exchanging a light source, a user performs filament centering, that is, filament centering adjustment.
Conventionally, in this type of filament centering adjustment, for example, a revolver-mounted type is known as centering adjustment when a mercury lamp (for fluorescence observation) is adopted as a light source in epi-illumination. As shown in FIG. 3, an objective lens mounting type centering screen is used in place of the objective lens as shown in FIG. 3, and a mounting screw to a revolver (not shown) is attached to one end of a cylindrical centering screen body 1. 2, a mirror 3 inside the main body 1, and a target screen 5 is arranged in the window 4 on the lower side surface of the main body 1, so that the optical path from the revolver side passes through the mirror 3 to the window 4 side. The filament core can be adjusted by the target screen 5 by being bent.
[0003]
In addition, as a microscope built-in type, a target screen 9 is incorporated between the revolver 7 and the eyepiece 8 of the microscope main body 6 as shown in FIG. 4, and a mirror (not shown) is inserted in the optical path of the microscope. At the time of center adjustment, a mirror is inserted into the optical path, so that light from the light source is introduced into the target screen 9 inside the main body 6, and the centering adjustment of the filament is enabled by this target screen 9.
[0004]
[Problems to be solved by the invention]
However, since the former requires a special centering screen body 1 to be prepared, it is expensive in price, and the centering screen body 1 having the target screen 5 is structurally recessed under the revolver. Therefore, it is difficult to see an image for centering adjustment on the target screen 5 through the window 4, so that the work accuracy is liable to be lowered, and since it is dedicated to epi-illumination, the center of transmitted illumination is centered. There was a problem that it could not be used.
[0005]
In the latter case, since the target screen 9 is incorporated in the microscope, the entire microscope is increased in size and is expensive in price. Further, for the transmitted illumination, the target screen is inserted in a predetermined optical path. However, since the target screens are arranged at different positions for incident light and transmission, the structure is complicated and expensive in terms of price.
[0006]
A halogen lamp (for bright-field / dark-field observation) is also used as the light source. However, the centering adjustment is performed in the same manner as in the case of the mercury lamp, and the above-described problems also apply. However, in the case of a halogen lamp, adjustment is not always necessary, and depending on the model, there is a configuration in which the adjustment mechanism is omitted.
[0007]
The present invention has been made in view of the above circumstances, application can be a transmission and epi both lighting, moreover aims to provide a target SCREEN excellent in workability simple centering configuration.
[0008]
[Means for Solving the Problems]
According to a first aspect of the present invention, there is provided a cross mark for aligning the center of the optical axis of the microscope with the cross mark placed on the surface of the plate-like body that is placed on the stage of the microscope and used for focusing the light source image. In the epi-illumination target screen formed with an index having a circle centered on the mark, the size of the circle is substantially the same as the size of the field of view of the objective lens used for centering, and includes at least the index The range is characterized in that a paint containing a fluorescent paint is applied.
According to a second aspect of the invention, in the first aspect of the invention, the paint is white penetrating ink.
[0009]
The invention according to claim 3 is a translucent plate-like body that is placed on the stage of a microscope and used by adjusting the focus of the light source image, and has a cross mark and a center of the cross mark on the surface thereof. Two indexes having a circle to be formed are formed side by side, and a range including one of the indexes is applied with a paint containing a fluorescent paint.
[0012]
【Example】
Hereinafter, an embodiment of the present invention will be described with reference to the drawings.
FIG. 1 shows a front view of a target screen applied to the present invention. In the figure, reference numeral 11 denotes a target screen body having translucency, and the screen body 11 is made of a plate-like body having the same shape as a known slide glass. The material of the screen body 11 is optimally resin, and for example, acrylic or polycarbonate is used.
[0013]
The left half of the target screen body 11 is formed in the opaque part 111 for epi-illumination, and the right half is formed in the transparent part 112 for transmitted illumination. Here, the opaque portion 111 in the left half of the target screen body 11 is configured as a white portion by mixing a penetrating ink with a fluorescent paint added thereto. Here, the fluorescent paint is added to the opaque part 111. The excitation light for fluorescence observation uses light of various wavelengths, and among these, green excitation light (G excitation) is visually recognized by the naked eye. This is because the fluorescent dye emits light because of poor properties.
[0014]
A concentric circle 12 having the size of the field of view of the objective lens (10 ×) is formed in the opaque portion 111 on the left half of the target screen body 11. In this case, the outer side of the thickness of the line of the concentric circle 12 is the super-wide diameter, and the inner side is the normal viewing diameter. Here, the super-wide diameter and the normal visual field diameter correspond to the wide-field type and the standard type, which are the types of visual field of the eyepiece. A cross mark 13 for determining the optical axis is formed at the center of the concentric circle 12.
[0015]
Further, a concentric circle 14 circumscribing the filament size of the objective lens (10 ×) is formed in the transparent portion 112 on the right half of the target screen body 11, and the objective lens (10 ×) is placed inside the concentric circle 14. A concentric circle 15 having the size of the visual field is formed. Also in this case, the outer side of the thickness of the line of the concentric circle 15 is the super-wide diameter, and the inner side is the normal viewing diameter. Further, a concentric circle 16 having the size of the field of view of the objective lens (4 ×) is formed inside the concentric circle 15. At the center of these concentric circles 14, 15 and 16, a cross mark 17 for determining the optical axis is formed.
[0016]
Thus, in order to adjust the centering of the filament using such a target screen, the target screen body 11 is set on a sample stage of a microscope (not shown). In this case, the setting of the target screen main body 11 on the stage of the microscope has the same shape as the slide glass, so that the mounting portion of the slide glass is used as it is and fixed.
[0017]
First, in order to perform centering adjustment when a mercury lamp is used as the light source with epi-illumination, an objective lens (10 ×) is attached to a revolver (not shown), and in this state, the left half of the target screen body 11 is Using the opaque portion 111, the approximate focusing and centering, that is, the optical axis alignment (the cross mark 13 is aligned with the center of the visual field) on the opaque portion 111 through the objective lens. Then, when the focusing and optical axis alignment are completed, the objective lens is removed from the optical axis. In this case, the objective lens may be removed, or a hole with no objective lens may be positioned by a revolver.
[0018]
From this state, the filament is first focused while viewing the image of the light source projected onto the opaque portion 111 on the left half of the target screen body 11. In this case, for example, the focusing is adjusted so that the filament image at the opaque portion 111 of the target screen body 11 becomes clear while moving the light source in the optical axis direction.
[0019]
Next, perform centering. The centering in this case is adjusted so that the filament image is positioned at the center of the cross mark 13 in the concentric circle 12 of the opaque portion 111 of the target screen body 11 while moving the light source in a plane perpendicular to the optical axis. To do.
[0020]
Note that the focusing and centering here are performed by, for example, an adjustment screw for axial alignment and focusing of a light source provided in a lamp house or the like.
2 (a) and 2 (b) show such a state of adjusting the centering of the filament. FIG. 2A shows a state in which the filament image F is shifted to the left side from the center of the cross mark 13 in the concentric circle 12 of the opaque portion 111 of the target screen main body 11. In this state, the filament image F is clear. Adjust the focus so that Next, while moving the light source in a plane perpendicular to the optical axis, adjustment is performed so that the filament image F is positioned at the center of the cross mark 13 in the concentric circle 12 of the opaque portion 111 as shown in FIG. To complete the filament centering adjustment.
[0021]
Next, in order to perform centering adjustment when a mercury lamp is used as the light source with transmitted illumination, the transparent portion 112 on the right half of the target screen body 11 is used, and the approximate focusing on the transparent portion 112 is performed. Align. The centering here is performed using a condenser lens of a microscope (not shown).
[0022]
From this state, the filament focusing and centering adjustment are performed in the same manner as described above while viewing the image of the light source projected on the transparent portion 112 on the right half of the target screen body 11.
[0023]
Therefore, according to such an embodiment, the left half of the target screen body 11 made of a translucent plate-like body is formed in the opaque portion 111 and the right half is formed in the transparent portion 112, and the cross mark is formed on the surface of the opaque portion 111. 13 and a concentric circle 12 centered on the cross mark 13, and a cross mark 17 and concentric circles 14, 15 and 16 centered on the cross mark 17 are formed on the surface of the transparent portion 112, respectively. Can be made simple and inexpensive. Moreover, by having the opaque part 111 for epi-illumination and the transparent part 112 for trans-illumination on the target screen main body 11, it can be used for centering adjustment of both transmission and epi-illumination, which is extremely advantageous economically. .
[0024]
Furthermore, by placing the target screen body 11 on the sample stage of the microscope, the filament image can be viewed on the stage, so that the work for adjusting the centering can be simplified.
[0025]
In the embodiment, the case where a mercury lamp is employed as the light source has been described. However, a halogen lamp can be used similarly.
As mentioned above, although demonstrated based on the Example, the following invention is contained in this invention.
[0026]
(1) A target screen comprising a translucent plate-like body and having a cross mark and a circle centered on the cross mark formed on the surface thereof.
In this way, a cross mark and a circle centered on the cross mark are formed on the surface of the translucent plate-like body, so that the configuration of the screen can be simplified and the price can be reduced.
[0027]
(2) In the target screen described in (1), the translucent plate-like body has a transparent part for transmitted illumination and an opaque part for epi-illumination, and a cross mark is provided on each of the transparent part and the opaque part. A circle centering on the cross mark is formed.
[0028]
In this way, the translucent plate-shaped body has a transparent part for transmitted illumination and an opaque part for incident illumination, so that it can be used for centering adjustment of both transmission and incident light. Can be economically advantageous.
[0029]
(3) A target screen in which a cross mark and a circle centered on the cross mark are formed on the surface of a light-transmitting plate is placed on a sample stage of a microscope, and a light source of the microscope is projected onto the target screen. A filament centering adjustment method comprising adjusting the filament centering of the light source.
[0030]
In this way, by placing the target screen on the sample stage of the microscope, the filament image can be viewed on the stage, so that the work for centering adjustment can be simplified.
[0031]
【The invention's effect】
According to the present invention as mentioned above, it is possible to simplify the structure of the scan clean, Ru also inexpensive price basis. Moreover , it can be used for adjusting the center of both transmission and epi-illumination, which can be economically advantageous.
[0032]
Moreover, since the filament image can be seen on the stage by placing the target screen on the sample stage of the microscope, the operation for adjusting the centering can be simplified.
[Brief description of the drawings]
FIG. 1 is a front view showing a schematic configuration of a target screen according to an embodiment of the present invention.
FIG. 2 is a diagram for explaining the embodiment.
FIG. 3 is a diagram showing an example of a conventional filament centering adjustment.
FIG. 4 is a view showing an example of conventional filament centering adjustment.
[Explanation of symbols]
11 ... Target screen body,
111 ... Opaque part,
112 ... transparent part,
12 ... Concentric circles,
13 ... Cross mark,
14, 15, 16 ... concentric circles,
17 ... Cross mark.

Claims (3)

Place a cross mark and a circle centered on the cross mark on the surface of the plate that is placed on the stage of the microscope and used for focusing the light source image. In the target screen for epi-illumination that has an indicator having,
A target screen in which the size of the circle is substantially the same as the size of the field of view of an objective lens used for centering, and a paint containing a fluorescent paint is applied in a range including at least the index.   The target screen according to claim 1, wherein the paint is a white penetrating ink. An indicator having a cross mark and a circle centered on the cross mark is formed on a surface of the light-transmitting plate-like body that is placed on the stage of the microscope and used by focusing the light source image. A target screen characterized by being formed side by side and applying a range including one of the indicators with a paint containing a fluorescent paint.

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