JP3637420B2 - F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 - Google Patents
F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 Download PDFInfo
- Publication number
- JP3637420B2 JP3637420B2 JP55057998A JP55057998A JP3637420B2 JP 3637420 B2 JP3637420 B2 JP 3637420B2 JP 55057998 A JP55057998 A JP 55057998A JP 55057998 A JP55057998 A JP 55057998A JP 3637420 B2 JP3637420 B2 JP 3637420B2
- Authority
- JP
- Japan
- Prior art keywords
- pigs
- coli
- resistant
- porcine
- fut1
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000282887 Suidae Species 0.000 title claims abstract description 80
- 241000588724 Escherichia coli Species 0.000 title claims abstract description 49
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 26
- 201000010099 disease Diseases 0.000 title claims abstract description 20
- 239000000203 mixture Substances 0.000 title abstract description 6
- 102100039835 Galactoside alpha-(1,2)-fucosyltransferase 1 Human genes 0.000 claims abstract description 47
- 101000893710 Homo sapiens Galactoside alpha-(1,2)-fucosyltransferase 2 Proteins 0.000 claims abstract description 44
- 241000282898 Sus scrofa Species 0.000 claims abstract description 36
- 102100040837 Galactoside alpha-(1,2)-fucosyltransferase 2 Human genes 0.000 claims abstract description 28
- 239000002773 nucleotide Substances 0.000 claims abstract description 25
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 25
- 229920001184 polypeptide Polymers 0.000 claims abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 4
- 108020004414 DNA Proteins 0.000 claims description 35
- 241001465754 Metazoa Species 0.000 claims description 29
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 16
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 16
- 229930024421 Adenine Natural products 0.000 claims description 14
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 14
- 229960000643 adenine Drugs 0.000 claims description 14
- 230000001488 breeding effect Effects 0.000 claims description 14
- 102000053602 DNA Human genes 0.000 claims description 12
- 238000009395 breeding Methods 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 12
- 230000000968 intestinal effect Effects 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 239000012472 biological sample Substances 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 108010019236 Fucosyltransferases Proteins 0.000 claims description 3
- 206010071602 Genetic polymorphism Diseases 0.000 claims description 3
- 238000011161 development Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- 230000009261 transgenic effect Effects 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 101000885616 Homo sapiens Galactoside alpha-(1,2)-fucosyltransferase 1 Proteins 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 229940113082 thymine Drugs 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 25
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract description 17
- 206010030113 Oedema Diseases 0.000 abstract description 12
- 238000012360 testing method Methods 0.000 abstract description 12
- 125000003275 alpha amino acid group Chemical group 0.000 abstract description 11
- 238000003556 assay Methods 0.000 abstract description 11
- 108010079306 galactoside 2-alpha-L-fucosyltransferase Proteins 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 206010012735 Diarrhoea Diseases 0.000 abstract description 6
- 238000006467 substitution reaction Methods 0.000 abstract description 5
- 208000028774 intestinal disease Diseases 0.000 abstract description 4
- 208000015181 infectious disease Diseases 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 description 38
- 210000004369 blood Anatomy 0.000 description 18
- 239000008280 blood Substances 0.000 description 18
- 108700028369 Alleles Proteins 0.000 description 17
- 230000035772 mutation Effects 0.000 description 17
- 108700026244 Open Reading Frames Proteins 0.000 description 16
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 11
- 239000000427 antigen Substances 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 11
- 108020003175 receptors Proteins 0.000 description 11
- 102000005962 receptors Human genes 0.000 description 11
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 11
- 238000012163 sequencing technique Methods 0.000 description 11
- 238000003752 polymerase chain reaction Methods 0.000 description 10
- 101150048676 FUT1 gene Proteins 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 102000054766 genetic haplotypes Human genes 0.000 description 7
- BCQZXOMGPXTTIC-UHFFFAOYSA-N halothane Chemical compound FC(F)(F)C(Cl)Br BCQZXOMGPXTTIC-UHFFFAOYSA-N 0.000 description 7
- 229960003132 halothane Drugs 0.000 description 7
- 241000255969 Pieris brassicae Species 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 230000006798 recombination Effects 0.000 description 5
- 102000042094 ryanodine receptor (TC 1.A.3.1) family Human genes 0.000 description 5
- 108091052345 ryanodine receptor (TC 1.A.3.1) family Proteins 0.000 description 5
- 230000003248 secreting effect Effects 0.000 description 5
- 208000035240 Disease Resistance Diseases 0.000 description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 4
- 108091008109 Pseudogenes Proteins 0.000 description 4
- 102000057361 Pseudogenes Human genes 0.000 description 4
- 238000002105 Southern blotting Methods 0.000 description 4
- 230000001464 adherent effect Effects 0.000 description 4
- 230000005757 colony formation Effects 0.000 description 4
- 238000002405 diagnostic procedure Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 210000000813 small intestine Anatomy 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 208000011580 syndromic disease Diseases 0.000 description 4
- 101150084750 1 gene Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 101150010169 FUT2 gene Proteins 0.000 description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000010065 bacterial adhesion Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000001842 enterocyte Anatomy 0.000 description 3
- 230000002550 fecal effect Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000007901 in situ hybridization Methods 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000031864 metaphase Effects 0.000 description 3
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
- 230000008707 rearrangement Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 108010090763 Shiga Toxin 2 Proteins 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000037429 base substitution Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 125000000837 carbohydrate group Chemical group 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 210000000110 microvilli Anatomy 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 238000000159 protein binding assay Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- BUOYTFVLNZIELF-UHFFFAOYSA-N 2-phenyl-1h-indole-4,6-dicarboximidamide Chemical compound N1C2=CC(C(=N)N)=CC(C(N)=N)=C2C=C1C1=CC=CC=C1 BUOYTFVLNZIELF-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 206010003591 Ataxia Diseases 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 208000034309 Bacterial disease carrier Diseases 0.000 description 1
- 101100067450 Caenorhabditis elegans fut-2 gene Proteins 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710146739 Enterotoxin Proteins 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 102000006471 Fucosyltransferases Human genes 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000051366 Glycosyltransferases Human genes 0.000 description 1
- 108700023372 Glycosyltransferases Proteins 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 101100227969 Homo sapiens FUT1 gene Proteins 0.000 description 1
- 206010021113 Hypothermia Diseases 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101100270435 Mus musculus Arhgef12 gene Proteins 0.000 description 1
- 230000004988 N-glycosylation Effects 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 210000001224 bacterial fimbriae Anatomy 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009582 blood typing Methods 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000002498 deadly effect Effects 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 208000022602 disease susceptibility Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 208000029182 enterotoxemia Diseases 0.000 description 1
- 239000000147 enterotoxin Substances 0.000 description 1
- 231100000655 enterotoxin Toxicity 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000000267 erythroid cell Anatomy 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 125000000404 glutamine group Chemical class N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 230000002631 hypothermal effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002614 leucines Chemical class 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 210000001537 mesocolon Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4718197P | 1997-05-20 | 1997-05-20 | |
| US60/047,181 | 1997-05-20 | ||
| PCT/US1998/010318 WO1998053102A1 (en) | 1997-05-20 | 1998-05-20 | Methods and compositions to identify swine genetically resistant to f18 e. coli associated diseases |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004299458A Division JP3941956B2 (ja) | 1997-05-20 | 2004-10-13 | F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001521401A JP2001521401A (ja) | 2001-11-06 |
| JP3637420B2 true JP3637420B2 (ja) | 2005-04-13 |
Family
ID=21947500
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55057998A Expired - Fee Related JP3637420B2 (ja) | 1997-05-20 | 1998-05-20 | F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 |
| JP2004299458A Expired - Fee Related JP3941956B2 (ja) | 1997-05-20 | 2004-10-13 | F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004299458A Expired - Fee Related JP3941956B2 (ja) | 1997-05-20 | 2004-10-13 | F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 |
Country Status (21)
| Country | Link |
|---|---|
| US (3) | US6596923B1 (enExample) |
| EP (1) | EP0985052B1 (enExample) |
| JP (2) | JP3637420B2 (enExample) |
| CN (1) | CN1314953A (enExample) |
| AT (2) | ATE309392T1 (enExample) |
| AU (2) | AU7580798A (enExample) |
| BR (1) | BR9809137A (enExample) |
| CA (1) | CA2290768C (enExample) |
| CZ (1) | CZ298021B6 (enExample) |
| DE (3) | DE69832306T2 (enExample) |
| DK (2) | DK0985052T3 (enExample) |
| ES (2) | ES2203958T3 (enExample) |
| HK (1) | HK1040097A1 (enExample) |
| HU (1) | HUP0103587A3 (enExample) |
| MX (1) | MXPA99011988A (enExample) |
| NO (1) | NO996373L (enExample) |
| NZ (1) | NZ501886A (enExample) |
| PL (1) | PL195890B1 (enExample) |
| PT (1) | PT985052E (enExample) |
| RU (1) | RU2204609C2 (enExample) |
| WO (2) | WO1998053102A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2204609C2 (ru) * | 1997-05-20 | 2003-05-20 | Биотехнолоджи Резёрч Энд Девелопмент Корпорэйшен | СПОСОБ ИДЕНТИФИКАЦИИ СВИНЕЙ, РЕЗИСТЕНТНЫХ К КИШЕЧНЫМ ЗАБОЛЕВАНИЯМ, АССОЦИИРОВАННЫМ С Е.Сoli, СПОСОБ РАЗВЕДЕНИЯ СВИНЕЙ, РЕЗИСТЕНТНЫХ К КИШЕЧНЫМ ЗАБОЛЕВАНИЯМ, АССОЦИИРОВАННЫМ С E.Coli, ВЫДЕЛЕННАЯ ДНК-МОЛЕКУЛА (ВАРИАНТЫ), МОЛЕКУЛЯРНЫЙ АНАЛИЗ ДЛЯ ОБНАРУЖЕНИЯ РЕЦЕПТОРОВ Е.Сoli F-18 У СВИНЕЙ И НАБОР ДЛЯ ОБНАРУЖЕНИЯ РЕЦЕПТОРОВ Е.Сoli F-18 |
| US6355859B1 (en) * | 1997-05-20 | 2002-03-12 | Biotechnology Research And Development Corporation | Interactions between genotype and diet in swine that prevent E. coli associated intestinal disease |
| HU221664B1 (hu) * | 1999-03-31 | 2002-12-28 | MTA Állatorvostudományi Kutatóintézete | Élő, szájon át adható Escherichia coli vakcina készítésére alkalmas törzs a sertések választási hasmenésének megelőzésére és a törzs előállítására alkalmas eljárás |
| GB0118549D0 (en) * | 2001-07-30 | 2001-09-19 | Pig Improvement Uk Ltd | Methods |
| US7785778B2 (en) | 2002-11-25 | 2010-08-31 | University Of Copenhagen | Porcine polymorphisms and methods for detecting them |
| BR0317329A (pt) | 2002-12-31 | 2005-11-08 | Mmi Genomics Inc | Composições, métodos e sistemas para inferir traços bovinos |
| CN104982387B (zh) * | 2015-05-25 | 2018-01-23 | 广东温氏食品集团股份有限公司 | 一种muc13和fut1基因的分子标记辅助选择方法 |
| CN108348585B (zh) * | 2015-09-02 | 2023-12-15 | 杜邦营养生物科学有限公司 | 糖苷水解酶及其在预防和/或治疗动物中的病原体感染中的用途 |
| CN106480174B (zh) * | 2016-09-14 | 2019-04-16 | 扬州大学 | 一种用于猪抗病育种的遗传分子标记方法和应用 |
| CN108384865A (zh) * | 2018-02-07 | 2018-08-10 | 扬州大学 | Alpha菌毛基因在仔猪腹泻致病性大肠杆菌监测、鉴别中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK41885A (da) | 1985-01-31 | 1986-11-13 | Slagteriernes Forskningsinst | 987p-fimbrie-producerende mikroorganisme, vaccine til immunisering af grise samt fremgangsmaade til fremstilling af vaccinen |
| EP0563144B2 (en) | 1990-12-21 | 2003-02-19 | The University Of Toronto Innovations Foundation | Diagnosis for porcine malignant hyperthermia |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US5552144A (en) | 1992-01-22 | 1996-09-03 | Microcarb, Inc. | Immunogenic shiga-like toxin II variant mutants |
| AU5715194A (en) | 1992-12-04 | 1994-07-04 | N.V. Innogenetics S.A. | New polypeptides, nucleic acid sequences encoding them, and their use to prevent the adhesion of f107-fimbriated bacteria |
| US5625124A (en) * | 1994-07-11 | 1997-04-29 | Washington University | Animal model for helicobacter pylori infection |
| WO1996028967A1 (en) | 1995-03-17 | 1996-09-26 | Chihiro Koike | Transgenic non-primatal mammals wherein serotypes of higher primates have been expressed by foreign gene transfer and method of creating the same |
| RU2204609C2 (ru) * | 1997-05-20 | 2003-05-20 | Биотехнолоджи Резёрч Энд Девелопмент Корпорэйшен | СПОСОБ ИДЕНТИФИКАЦИИ СВИНЕЙ, РЕЗИСТЕНТНЫХ К КИШЕЧНЫМ ЗАБОЛЕВАНИЯМ, АССОЦИИРОВАННЫМ С Е.Сoli, СПОСОБ РАЗВЕДЕНИЯ СВИНЕЙ, РЕЗИСТЕНТНЫХ К КИШЕЧНЫМ ЗАБОЛЕВАНИЯМ, АССОЦИИРОВАННЫМ С E.Coli, ВЫДЕЛЕННАЯ ДНК-МОЛЕКУЛА (ВАРИАНТЫ), МОЛЕКУЛЯРНЫЙ АНАЛИЗ ДЛЯ ОБНАРУЖЕНИЯ РЕЦЕПТОРОВ Е.Сoli F-18 У СВИНЕЙ И НАБОР ДЛЯ ОБНАРУЖЕНИЯ РЕЦЕПТОРОВ Е.Сoli F-18 |
| US6355859B1 (en) * | 1997-05-20 | 2002-03-12 | Biotechnology Research And Development Corporation | Interactions between genotype and diet in swine that prevent E. coli associated intestinal disease |
| US6569923B1 (en) * | 1999-03-19 | 2003-05-27 | John T. Slagter | Polymer-cement composites and methods of making same |
-
1998
- 1998-05-20 RU RU99127287/13A patent/RU2204609C2/ru not_active IP Right Cessation
- 1998-05-20 JP JP55057998A patent/JP3637420B2/ja not_active Expired - Fee Related
- 1998-05-20 HU HU0103587A patent/HUP0103587A3/hu unknown
- 1998-05-20 AT AT03001366T patent/ATE309392T1/de not_active IP Right Cessation
- 1998-05-20 HK HK02101688.3A patent/HK1040097A1/zh unknown
- 1998-05-20 DE DE69832306T patent/DE69832306T2/de not_active Expired - Fee Related
- 1998-05-20 CN CN98807387A patent/CN1314953A/zh active Pending
- 1998-05-20 MX MXPA99011988A patent/MXPA99011988A/es active IP Right Grant
- 1998-05-20 PT PT98923570T patent/PT985052E/pt unknown
- 1998-05-20 AU AU75807/98A patent/AU7580798A/en not_active Withdrawn
- 1998-05-20 CA CA002290768A patent/CA2290768C/en not_active Expired - Lifetime
- 1998-05-20 DK DK98923570T patent/DK0985052T3/da active
- 1998-05-20 WO PCT/US1998/010318 patent/WO1998053102A1/en not_active Ceased
- 1998-05-20 AU AU75832/98A patent/AU737862B2/en not_active Ceased
- 1998-05-20 EP EP98923570A patent/EP0985052B1/en not_active Expired - Lifetime
- 1998-05-20 WO PCT/US1998/010259 patent/WO1998053101A2/en not_active Ceased
- 1998-05-20 BR BR9809137-9A patent/BR9809137A/pt not_active Application Discontinuation
- 1998-05-20 ES ES98923570T patent/ES2203958T3/es not_active Expired - Lifetime
- 1998-05-20 ES ES03001366T patent/ES2248647T3/es not_active Expired - Lifetime
- 1998-05-20 PL PL98364701A patent/PL195890B1/pl unknown
- 1998-05-20 AT AT98923570T patent/ATE246731T1/de not_active IP Right Cessation
- 1998-05-20 CZ CZ0460799A patent/CZ298021B6/cs not_active IP Right Cessation
- 1998-05-20 NZ NZ501886A patent/NZ501886A/en unknown
- 1998-05-20 DE DE69816987T patent/DE69816987T2/de not_active Expired - Fee Related
- 1998-05-20 DE DE0985052T patent/DE985052T1/de active Pending
- 1998-05-20 DK DK03001366T patent/DK1310570T3/da active
-
1999
- 1999-11-19 US US09/443,766 patent/US6596923B1/en not_active Expired - Lifetime
- 1999-12-21 NO NO996373A patent/NO996373L/no unknown
-
2001
- 2001-04-27 US US09/844,705 patent/US6965022B2/en not_active Expired - Lifetime
- 2001-07-25 US US09/844,268 patent/US7193072B2/en not_active Expired - Fee Related
-
2004
- 2004-10-13 JP JP2004299458A patent/JP3941956B2/ja not_active Expired - Fee Related
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Meijerink et al. | Two α (1, 2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci | |
| AU2003281988B2 (en) | Porcine polymorphisms and methods for detecting them | |
| JP3637420B2 (ja) | F18イー・コリ関連疾患に対して遺伝学的に耐性のブタを同定する方法および組成物 | |
| US6355859B1 (en) | Interactions between genotype and diet in swine that prevent E. coli associated intestinal disease | |
| EP1310570B1 (en) | Methods and compositions to identify swine genetically resistant to F18 E. Coli associated diseases | |
| Sinha et al. | Effect of Mucin13 gene polymorphism on diarrhoeagenic E. coli adhesion pattern and its expression analysis in native Indian pigs | |
| Cohney | HOSvyOrth et al. | |
| Ridpath et al. | HOSvyOrth et al. | |
| KUMAR | DNA POLYMORPHISM OF MYOSTATIN GENE IN DIFFERENT MEAT AND NON-MEAT GOAT BREEDS | |
| Cauveri | ASSOCIATION OF SNPs IN EXON 3 OF LEPTIN (LEP) GENE WITH GROWTH TRAITS IN NILAGIRI SHEEP OF TAMIL NADU | |
| EP1485506A2 (en) | Method for identifying animals for milk production qualities by analyzing the polymorphism of the pit-1 and kappa-casein genes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20040713 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20041013 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20041207 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20041221 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R154 | Certificate of patent or utility model (reissue) |
Free format text: JAPANESE INTERMEDIATE CODE: R154 |
|
| LAPS | Cancellation because of no payment of annual fees |