JP3600073B2 - Improved microbial pesticide formulation - Google Patents
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Description
【０００１】
【発明の属する技術分野】
近年、植物病害防除の分野で、微生物農薬が生物的防除のための重要な手段として期待されている。これらの微生物農薬を実用化する際の大きな課題は、製造してからのち、流通、輸送、保管の段階を経て、使用する段階まで、農薬としての効果を示すに必要なだけの微生物の生存率、そして、活性を保った状態で保存し続ける必要があるというところにある。さらには、従来の化学農薬により近い、粉剤、水和剤のような形態で供給して、現場での散布方法、使用する器具、道具などの点について、可能な限り従来の化学農薬により近い方法で用い得るならば、なお好ましい。従来より、上述した剤形の微生物農薬としては、種々の無機あるいは高分子の担体に固定化したもの（本間善久、『農業及び園芸』第６９巻第２号、４２−４８、「拮抗微生物による土壌病害の生物的防除」）が知られているが、いずれも保存性という点では充分とは言い難い。また、非病原性フザリウム菌を種々の鉱物質系の資材に吸着し、製剤化したもの（小川圭ら、日植病報５３巻、４１６−４１７、「非病原性フザリウム菌の製剤化に関する研究」）やフザリウムの生菌体をゼオライト系の基材に吸着させ、自然乾燥させることで生菌としての活性と安定性とを同時に備えるようにした生菌製剤（特開昭６３−２２７５０７号公報）などが知られているが、常温下での長期保存により微生物の生存率が低下してしまうという問題があった。さらに、上記製剤の微生物が糸状菌のフザリウム菌に限定されており、フザリウム菌以外の特に胞子等の耐久体をつくらない細菌になると常温での保存性に優れた製剤の製造は、さらに困難な状況である。また、上述したように、通常、クレー、炭酸カルシウム、タルク、珪藻土、ゼオライトなどの鉱物系資材が微生物の固定化を主目的として用いられているので、製剤化は、培養後のウエットな状態の微生物と上記資材を混合した後に乾燥する方法が一般的にとられている。
【０００２】
また、微生物農薬の使用方法として、水和剤などの水に溶解させて使用するものも多くある。特に水道水を使用した場合は、その残留塩素により微生物が死滅する場合があり、有用な微生物を十分に働かせることが困難なことがある。
【０００３】
【発明が解決しようとする課題】
本発明は、塩素除去能、酸化防止能を有する物質を植物病害に対して防除効果を有する微生物を凍結乾燥させる前または凍結乾燥させた後に混合させることにより、保存安定性、特に水道水への溶解後の安定性に優れた微生物農薬製剤を提供することを課題とする。
【０００４】
【課題を解決するための手段】
本発明者らは、鋭意検討の結果、植物病害に対して防除効果を有する微生物に亜硫酸塩、チオ硫酸塩から選ばれる少なくとも１種類の物質を混合した微生物農薬製剤を製造することで、微生物の保存性、特に水道水への溶解後の安定性を向上させることができることを見出し、本発明に到達した。
【０００５】
すなわち、本発明は、植物病害に対して防除効果を有する微生物に亜硫酸塩、チオ硫酸塩から選ばれる少なくとも１種類の物質を混合させたことを特徴とする植物病害に対して防除効果を有する微生物農薬製剤であり、植物病害に対して防除効果を有する微生物が特にグラム陰性細菌であること、また、グラム陰性細菌がエルビニア属細菌あるいはシュードモナス属細菌であることを特徴とする微生物農薬製剤である。
【０００６】
以下、本発明を詳細に説明する。
【０００７】
本発明者らは、鋭意検討の結果、エルビニア・カロトボーラ細菌により引き起こされる、難防除な植物病害の一種である軟腐病を、軟腐病の病原性を欠失させて非病原化したエルビニア・カロトボーラ細菌を用いることにより、有効に防除することができることを提案した（特公平６−９２２８６号公報）。さらに、非病原化したエルビニア・カロトボーラ細菌として、より広範な菌株に対して優れた抗菌活性を示す非病原性エルビニア・カロトボーラ サブスピ カロトボーラＣＧＥ２３４Ｍ４０３菌株（Ｅｒｗｉｎｉａ ｃａｒｏｔｏｖｏｒａ ｓｕｂｓｐ． ｃａｒｏｔｏｖｏｒａ ＣＧＥ２３４Ｍ４０３）を用いることについても提案している（特公平６−３８７４６号公報）。また、該菌株を種々の糖類と混合し、真空凍結乾燥することで保存性に優れた微生物農薬を製造できることを見出し、特許出願した（特開平４−３１１３９１号公報）。さらに、微生物農薬の製造工程において、該菌株をアスコルビン酸とともに凍結乾燥させることで、良好な保存性を得ることができることも見出し、特許出願した（特開平８−１８３７０６号公報）。
【０００８】
一方、シュードモナス属菌であるシュードモナス・エスピー ＣＡＢ−０２菌株（Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ． ＣＡＢ−０２）は、イネもみ枯細菌病菌による苗腐敗症、イネ苗立枯細菌病およびイネばか苗病の３病害を同時に防除することが可能であり、特許出願されている（特開平９−１２４４２７号公報）。さらに、該菌株を糖類と混合し、真空凍結乾燥もしくは真空乾燥することによって生存率を維持させ、長期間にわたって安定に保存することができることを見出し、特許出願した（特願平１０−０８３５１８号）。上述した軟腐病およびイネ病害に対して防除効果の高い菌株は、以下の如く、工業技術院生命工学工業技術研究所に寄託され、以下の寄託番号が付与されている。
【０００９】
【００１０】
そこで、上述した問題を解決すべく検討した結果、常圧で包装することが可能となり、かつ、常温での保存性も向上できる方法を見出した。すなわち、ゼオライト、モレキュラシーブス、シリカゲルのようなアンモニア吸着能を有する吸着剤を製剤に混合する方法により、保存中に発生するアンモニアガスを吸着・除去し、常圧包装でも菌を長期間、安定に保存することが可能となった（特願平１１−７０５９９号、特願平１１−７０６００号）。
【００１１】
しかしながら、微生物農薬の使用方法として、水和剤などの水に溶解させて使用するものも多くあり、上述した２菌株をそれぞれ有効成分とする微生物農薬製剤も水和剤として使用することが可能であるが、特に水道水を使用した場合には、その残留塩素により微生物が死滅する場合があり、微生物を十分に働かせることが困難なことがあるという新たな問題が発生した。
【００１２】
そこで、この新たな問題を解決すべく検討した結果、亜硫酸ナトリウム、亜硫酸カリウム、亜硫酸アンモニウムなどの亜硫酸塩、あるいはチオ硫酸ナトリム、チオ硫酸カリウム、チオ硫酸アンモニウムなどのチオ硫酸塩のような塩素を除去する能力を有する物質を添加することにより、水道水への溶解時の微生物の生存性を向上させ、安定性を向上させることができることを見出したものである。
【００１３】
【発明の実施の形態】
以下、本発明の実施の形態を実施例により具体的に説明する。まず、非病原性エルビニア・カロトボーラ サブスピ カロトボーラ ＣＧＥ２３４Ｍ４０３菌株あるいはシュードモナス・エスピー ＣＡＢ−０２菌株を適当な培地で培養する。ここで使用される培地は、菌が増殖するものであれば、特に限定されるものではない。必要な炭素源、窒素源、無機物などを適当に含有していて、菌の生育が可能な培地であれば、天然培地、合成培地のいずれも用いることができる。培地としては、具体的には、例えば、８０２培地、ブイヨン培地、キングＢ培地、ＰＳ培地、ＰＤＢ培地、合成Ｍ９培地などが挙げられる。以上のような培地で、非病原性エルビニア・カロトボーラ サブスピ カロトボーラ ＣＧＥ２３４Ｍ４０３菌株は、１５〜３７℃、好ましくは２０〜３０℃で、シュードモナス・エスピー ＣＡＢ−０２菌株は、１５〜４２℃、好ましくは３０〜４０℃で、１０〜３５時間培養し、増殖させた後に遠心分離もしくは限外ろ過膜による濃縮により菌体を回収する。次に、回収した菌体を、保護液に懸濁し、凍結した後、凍結乾燥機により乾燥する。
【００１４】
本発明においては、上記の凍結乾燥前に亜硫酸塩またはチオ硫酸塩を混合してもよい。この場合、ここで回収した菌体に亜硫酸塩またはチオ硫酸塩を混合してもよいし、保護液に亜硫酸塩またはチオ硫酸塩を混合してもよい。
【００１５】
得られた乾燥菌体を粉砕し、菌濃度を一定にするために増量剤と混合して製剤を作成する。増量剤としては、タルク、珪藻土、炭酸カルシウムなどの鉱物性粉末などがあり、水和剤として要求される重要な品質特性である、水で希釈するときに薬剤が速やかに水になじみ、それを撹拌したときに分散性が良好で、しかもそれを長く維持するもので、なおかつ、菌濃度を落とすものでなければ、これらに限定されるものではない。
【００１６】
また、本発明においては、この凍結乾燥後の製剤に亜硫酸塩またはチオ硫酸塩を混合して微生物農薬製剤を製造してもよい。混合する亜硫酸塩またはチオ硫酸塩としては少なくとも１種類を用いる。
【００１７】
亜硫酸塩としては、亜硫酸ナトリウム、亜硫酸カリウム、亜硫酸アンモニウムなどがあり、また、チオ硫酸塩としては、チオ硫酸ナトリウム、チオ硫酸カリウム、チオ硫酸アンモニウムなどがある。製剤に対して添加する量は、０．０２重量％〜５．０重量％が好ましく、より好ましくは０．２重量％〜１．０重量％とするのが、微生物の生存性向上にとってよい。塩素除去能を有する物質の添加量が０．０２重量％以下または５．０重量％以上では、充分な生存性を得ることができなくなる場合があるため、好ましくない。
【００１８】
本発明における亜硫酸塩あるいはチオ硫酸塩などの塩素除去能を有する物質の混合は、上述のように乾燥または凍結乾燥前に混合しても、乾燥または凍結乾燥後に混合してもよいが、さらに原体や製剤化後の製剤のいずれに混合しても良い。
【００１９】
以上のような本発明の方法により、水、特に水道水への溶解時の微生物の生存性を向上させ、安定性を向上させることができた。つまり、亜硫酸塩（亜硫酸ナトリウム、亜硫酸カリウム、亜硫酸アンモニウムなど）あるいはチオ硫酸塩（チオ硫酸ナトリム、チオ硫酸カリウム、チオ硫酸アンモニウムなど）のような塩素を除去する能力を有する物質により、水道水への溶解時の安定性を向上させることができた。
【００２０】
【実施例】
次に実施例を示すが、本発明は以下の実施例によって限定されるものではない。
【００２１】
なお、実施例に用いた培地の組成を次に示す。
【００２２】
８０２培地：ポリペプトン １０ｇ、酵母エキス ２ｇ、ＭｇＳＯ４・７Ｈ２Ｏ １ｇ、水 １Ｌ、ｐＨ７．２
実施例１
まず、非病原性エルビニア・カロトボーラ サブスピ カロトボーラ ＣＧＥ２３４Ｍ４０３菌株（ＦＥＲＭ ＢＰ−４３２８）を２００ｍｌの８０２培地に接種し、前培養した後、４Ｌの８０２培地でジャーファーメンターにより、３０時間培養した。培養後、遠心分離機による遠心分離にて、菌体を回収し、保護液に懸濁した。この保護液に亜硫酸ナトリウムを製剤調整後の濃度が２．０重量％の濃度になるように加えた。この懸濁液を凍結した後、凍結乾燥機により２日間乾燥した。乾燥菌体を粉砕し、所定量の増量剤および亜硫酸ナトリウムと混合した後、常圧包装した。このようにして、微生物農薬製剤を製造した。その後、３７℃にて保存し、水に溶解した際の微生物農薬製剤中の菌の生存率を経時的に測定した。その結果を表１に示す。なお、製剤の溶解水には、イオン交換水（ＩＥＷ）および水道水を用いた。
【００２３】
比較例１
実施例１と全く同様にして、ただし、亜硫酸ナトリウムを混合しない微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表１に示す。
【００２４】
【表１】
実施例１と全く同様にして、ただし、亜硫酸ナトリウムを乾燥菌体を粉砕し、所定量の増量剤と混合した後、１．０重量％となるように混合した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表２に示す。
【００２５】
比較例２
実施例２と全く同様にして、ただし、亜硫酸ナトリウムを混合しない微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表２に示す。
【００２６】
【表２】
実施例２と全く同様にして、ただし、非病原性エルビニア・カロトボーラ サブスピ カロトボーラ ＣＧＥ２３４Ｍ４０３菌株の代わりにシュードモナス・エスピー ＣＡＢ−０２菌株（ＦＥＲＭ Ｐ−１５２３７）を培養して微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表３に示す。
【００２７】
比較例３
実施例３と全く同様にして、ただし、亜硫酸ナトリウムを混合しない微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表３に示す。
【００２８】
【表３】
実施例３と全く同様にして、ただし、亜硫酸ナトリウムの代わりにチオ硫酸ナトリウムを混合した微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表４に示す。
【００２９】
比較例４
実施例４と全く同様にして、ただし、チオ硫酸ナトリウムを混合しない微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表４に示す。
【００３０】
【表４】
実施例３と全く同様にして、１．０重量％の亜硫酸ナトリウムを混合したものに加えて、それぞれ０重量％、５．０重量％の亜硫酸ナトリウムを混合した微生物農薬製剤を製造した。その後、実施例１と同様にして菌の生存率を測定した。その結果を表５に示す。
【００３１】
【表５】
【発明の効果】
本発明により、微生物農薬製剤を水、特に水道水に溶解した場合に生じる水道水中の残留塩素による微生物の死滅もしくは菌濃度低下を防ぐことができる。[0001]
TECHNICAL FIELD OF THE INVENTION
BACKGROUND ART In recent years, in the field of plant disease control, microbial pesticides are expected as important means for biological control. The major challenge in putting these microbial pesticides into practical use is the survival rate of the microbes that is necessary to exhibit the effect as a pesticide from production, distribution, transportation, storage, to the stage of use. And, it is necessary to keep storing while maintaining the activity. Furthermore, it is supplied in the form of powders and wettable powders, which are closer to conventional chemical pesticides. It is even more preferred if it can be used in Conventionally, microbial pesticides in the above-mentioned dosage form include those immobilized on various inorganic or polymeric carriers (Honma Yoshihisa, "Agriculture and Horticulture" Vol. 69, No. 2, 42-48, " Biological control of soil diseases ") is known, but none of them is sufficient in terms of preservability. In addition, non-pathogenic Fusarium bacteria are adsorbed to various mineral-based materials and formulated (Kei Ogawa et al., Nikkei Disease Report 53, 416-417, "Study on formulation of non-pathogenic Fusarium bacteria" )) And a live cell preparation prepared by adsorbing live cells of fusarium onto a zeolite-based substrate and air-drying to simultaneously provide the activity and stability as live cells (Japanese Patent Application Laid-Open No. 63-227507). ) Is known, but there is a problem that the long-term storage at room temperature lowers the survival rate of microorganisms. Furthermore, the microorganism of the above-mentioned preparation is limited to the filamentous fungus Fusarium bacterium, and when it becomes a bacterium that does not form a durable body such as spores other than Fusarium bacterium, it is more difficult to produce a preparation excellent in storability at room temperature. The situation. In addition, as described above, usually, mineral materials such as clay, calcium carbonate, talc, diatomaceous earth, and zeolite are mainly used for immobilizing microorganisms, so that formulation is performed in a wet state after culture. A method is generally employed in which the microorganism is mixed with the above-mentioned materials and then dried.
[0002]
As a method of using a microbial pesticide, there are many methods in which the microbial pesticide is dissolved in water such as a wettable powder. In particular, when tap water is used, microorganisms may be killed by residual chlorine, and it may be difficult to make useful microorganisms work sufficiently.
[0003]
[Problems to be solved by the invention]
The present invention provides a storage stability, especially for tap water, by mixing a substance having a chlorine removing ability and an antioxidant ability before or after freeze-drying a microorganism having a controlling effect on plant diseases. An object of the present invention is to provide a microbial pesticide preparation having excellent stability after dissolution.
[0004]
[Means for Solving the Problems]
The present inventors have conducted intensive studies and, as a result, produced a microbial pesticide formulation in which at least one substance selected from sulfites and thiosulfates was mixed with a microorganism having a controlling effect on plant diseases, The present inventors have found that the storage stability, particularly the stability after dissolution in tap water, can be improved, and have reached the present invention.
[0005]
That is, the present invention provides a microorganism having a controlling effect on plant diseases, characterized by mixing at least one substance selected from sulfites and thiosulfates with a microorganism having a controlling effect on plant diseases. A microbial pesticide preparation which is a pesticide preparation, wherein the microorganism having a controlling effect on plant diseases is a gram-negative bacterium, and the gram-negative bacterium is a genus Erwinia or a genus Pseudomonas.
[0006]
Hereinafter, the present invention will be described in detail.
[0007]
The present inventors have conducted intensive studies and found that Erwinia carotobola bacteria, which are caused by Erwinia carotobora bacteria, are soft rots, which are a kind of intractable plant diseases, and are made nonpathogenic by deleting the pathogenicity of soft rot. It has been proposed that the use of a compound enables effective control (Japanese Patent Publication No. 6-92286). Furthermore, as a non-pathogenic Erwinia carotovora bacterium, it is also proposed to use a non-pathogenic Erwinia carotovora subsp. Carotovora CGE234M403 strain ( Erwinia carotovora subsp. Carotovora CGE234M403) which exhibits excellent antibacterial activity against a wider range of strains. (Japanese Patent Publication No. 6-38746). Further, they have found that a microbial pesticide excellent in storability can be produced by mixing the strain with various saccharides and freeze-drying in a vacuum, and applied for a patent (Japanese Patent Application Laid-Open No. 4-311391). Furthermore, in the production process of the microbial pesticide, it has been found that good preservability can be obtained by freeze-drying the strain together with ascorbic acid, and a patent application has been filed (Japanese Patent Application Laid-Open No. 8-183706).
[0008]
On the other hand, Pseudomonas sp. CAB-02 strain ( Pseudomonas sp. CAB-02), which is a genus Pseudomonas, simultaneously inhibits three diseases of seedling rot, rice seedling blight bacterial disease and rice blight seedling disease caused by rice germ blight. It can be controlled, and a patent application has been filed (JP-A-9-124427). Furthermore, the inventors have found that the viability can be maintained by mixing the strain with a saccharide and freeze-drying or vacuum-drying, and that the strain can be stably stored for a long period of time, and a patent application has been filed (Japanese Patent Application No. 10-083518). . Strains having a high control effect on the above-mentioned soft rot and rice disease are deposited with the National Institute of Advanced Industrial Science and Technology as follows, and given the following deposit numbers.
[0009]
[0010]
Then, as a result of studying to solve the above-described problems, a method was found that enables packaging under normal pressure and also improves the storage stability at normal temperature. In other words, by adsorbing and removing ammonia gas generated during storage by a method of mixing an adsorbent having an ammonia adsorption capacity such as zeolite, molecular sieves, and silica gel into a preparation, bacteria can be stably stuck for a long time even under normal pressure packaging. It can be stored (Japanese Patent Application No. 11-70599, Japanese Patent Application No. 11-70600).
[0011]
However, many microbial pesticides are used by dissolving them in water such as wettable powders, and microbial pesticide preparations containing the above two strains as active ingredients can also be used as wettable powders. However, particularly when tap water is used, microorganisms may be killed by the residual chlorine, and a new problem arises that it may be difficult to make the microorganisms work sufficiently.
[0012]
Therefore, as a result of studying to solve this new problem, chlorine such as sodium sulphite, potassium sulphite, ammonium sulphite and other sulphite salts or sodium thiosulphate, potassium thiosulphate and ammonium thiosulphate thiosulphate are removed. It has been found that by adding a substance having a capability, the viability of microorganisms when dissolved in tap water can be improved, and the stability can be improved.
[0013]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, embodiments of the present invention will be specifically described with reference to examples. First, a non-pathogenic Erwinia carotobora subsp. Carotobora CGE234M403 strain or Pseudomonas sp. CAB-02 strain is cultured in an appropriate medium. The medium used here is not particularly limited as long as the bacteria can grow. Any of a natural medium and a synthetic medium can be used as long as the medium appropriately contains necessary carbon sources, nitrogen sources, inorganic substances and the like and can grow bacteria. Specific examples of the medium include 802 medium, bouillon medium, King B medium, PS medium, PDB medium, and synthetic M9 medium. In the medium as described above, the non-pathogenic Erwinia carotobola subspica carotobola CGE234M403 strain is at 15 to 37 ° C, preferably 20 to 30 ° C, and the Pseudomonas sp. CAB-02 strain is at 15 to 42 ° C, preferably 30 to 42 ° C. After culturing at 40 ° C. for 10 to 35 hours and growing, the cells are collected by centrifugation or concentration by an ultrafiltration membrane. Next, the collected cells are suspended in a protective solution, frozen, and then dried by a freeze dryer.
[0014]
In the present invention, a sulfite or a thiosulfate may be mixed before the freeze-drying. In this case, it may be mixed here recovered sulfite or thiosulfate cells, it may be mixed sulfite or thiosulfate protection liquid.
[0015]
The obtained dried cells are pulverized and mixed with a bulking agent to make the concentration of the cells constant to prepare a preparation. Examples of bulking agents include mineral powders such as talc, diatomaceous earth, and calcium carbonate, which are important quality characteristics required for wettable powders. The dispersibility is not limited to these as long as the dispersibility is good when stirred and the dispersibility is maintained for a long time, and the bacteria concentration is not reduced.
[0016]
In the present invention, a microbial pesticide preparation may be produced by mixing a sulfite or a thiosulfate with the preparation after freeze-drying. At least one type of sulfite or thiosulfate to be mixed is used.
[0017]
Examples of the sulfite include sodium sulfite, potassium sulfite, and ammonium sulfite, and examples of the thiosulfate include sodium thiosulfate, potassium thiosulfate, and ammonium thiosulfate . The amount added to the preparation is preferably 0.02% by weight to 5.0% by weight, more preferably 0.2% by weight to 1.0% by weight, to improve the viability of the microorganism. If the added amount of the substance having a chlorine removing ability is 0.02% by weight or less or 5.0% by weight or more, sufficient viability may not be obtained, which is not preferable.
[0018]
The mixing of substances having a chlorine removing ability such as sulfite or thiosulfate in the present invention may be performed before drying or freeze-drying as described above, or after dry or freeze-drying. It may be mixed with either the body or the formulation after formulation.
[0019]
According to the method of the present invention as described above, the viability of microorganisms when dissolved in water, particularly tap water, can be improved, and the stability can be improved. In other words, a substance having the ability to remove chlorine such as sulfite (sodium sulfite, potassium sulfite, ammonium sulfite, etc.) or thiosulfate (sodium sodium thiosulfate, potassium thiosulfate, ammonium thiosulfate, etc.) dissolves in tap water. The stability at the time was able to be improved.
[0020]
【Example】
Next, examples are shown, but the present invention is not limited to the following examples.
[0021]
The composition of the medium used in the examples is shown below.
[0022]
802 Medium: polypeptone 10 g, yeast extract 2g, MgSO 4 · 7H 2 O 1g, water 1L, pH 7.2
Example 1
First, non-pathogenic Erwinia carotobola subspica carotobola CGE234M403 strain (FERM BP-4328) was inoculated into 200 ml of 802 medium, pre-cultured, and then cultured in 4 L of 802 medium by jar fermenter for 30 hours. After the culture, the cells were collected by centrifugation using a centrifuge, and suspended in a protective solution. Sodium sulfite was added to this protective solution so that the concentration after preparation was 2.0% by weight. After freezing this suspension, it was dried for 2 days using a freeze dryer. The dried cells were pulverized, mixed with a predetermined amount of a bulking agent and sodium sulfite, and then packaged under normal pressure. Thus, a microbial pesticide formulation was produced. Thereafter, the cells were stored at 37 ° C. and the survival rate of the bacteria in the microbial pesticide formulation when dissolved in water was measured over time. Table 1 shows the results. In addition, ion-exchanged water (IEW) and tap water were used as the dissolution water of the preparation.
[0023]
Comparative Example 1
A microbial pesticide formulation was produced in exactly the same manner as in Example 1, except that sodium sulfite was not mixed. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 1 shows the results.
[0024]
[Table 1]
Exactly the same as in Example 1, except that sodium sulfite was pulverized into dried cells, mixed with a predetermined amount of a bulking agent, and then mixed to 1.0% by weight. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 2 shows the results.
[0025]
Comparative Example 2
A microbial pesticide formulation was produced in exactly the same manner as in Example 2, except that sodium sulfite was not mixed. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 2 shows the results.
[0026]
[Table 2]
Pseudomonas sp. CAB-02 strain (FERM P-15237) was cultured in exactly the same manner as in Example 2 except that the non-pathogenic Erwinia carotobola subspicatocarbora CGE234M403 strain was cultured to produce a microbial pesticide formulation. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 3 shows the results.
[0027]
Comparative Example 3
A microbial pesticide formulation was produced in exactly the same manner as in Example 3, except that sodium sulfite was not mixed. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 3 shows the results.
[0028]
[Table 3]
A microbial pesticide formulation was prepared in exactly the same manner as in Example 3, except that sodium thiosulfate was mixed instead of sodium sulfite. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 4 shows the results.
[0029]
Comparative Example 4
A microbial pesticide formulation was produced in exactly the same manner as in Example 4, except that sodium thiosulfate was not mixed. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 4 shows the results.
[0030]
[Table 4]
In exactly the same manner as in Example 3, microbial pesticide preparations were prepared in which 1.0% by weight of sodium sulfite was added, and 0% by weight and 5.0% by weight of sodium sulfite were mixed. Thereafter, the survival rate of the bacteria was measured in the same manner as in Example 1. Table 5 shows the results.
[0031]
[Table 5]
【The invention's effect】
Advantageous Effects of Invention According to the present invention, it is possible to prevent killing of microorganisms or reduction in bacterial concentration due to residual chlorine in tap water generated when a microbial pesticide formulation is dissolved in water, particularly tap water.
Claims (6)
Priority Applications (1)
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| JP20052899A JP3600073B2 (en) | 1999-07-14 | 1999-07-14 | Improved microbial pesticide formulation |
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| JP20052899A JP3600073B2 (en) | 1999-07-14 | 1999-07-14 | Improved microbial pesticide formulation |
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| JP2001031513A JP2001031513A (en) | 2001-02-06 |
| JP3600073B2 true JP3600073B2 (en) | 2004-12-08 |
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| JP4564242B2 (en) | 2003-05-30 | 2010-10-20 | 株式会社荏原製作所 | Treatment method, treatment agent and treatment apparatus for exhaust gas containing inorganic halogenated gas containing chlorine trifluoride |
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