JP3522264B2 - Method for producing xylo-oligosaccharide - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for producing a xylooligosaccharide useful as a sweetener, which is prepared from corn cob meal.

[0002]

BACKGROUND OF THE INVENTION Xylooligosaccharides are useful sugars used in lactic acid beverages, chocolates, etc., which are certified as foods for specified health uses, which have the function of maintaining a good condition of the stomach through the selective growth promoting effect of intestinal bacteria. And medicine,
In the field of sanitary products, it is also used as an emulsifying agent and a skin moisturizing ingredient. Further, it is used not only for human foods but also as an additive for animal feed.

In general, most of oligosaccharides used for specified health foods have an intestinal regulating action, that is, the number of Escherichia coli which is an "intestinal bad bacterium" and the number of bacteria of the genus Clostridium which is an intestinal spoilage fermentation bacterium are reduced, It has the effect of increasing the amount of bifidobacteria, which is said to be "good bacteria in the intestine". For example, wheat bran is a polysaccharide composed of hemicellulose having xylan as a main chain, and is added to foods as a food having an intestinal regulating action as a persistent fiber.

A large amount of xylose, which is a raw material for xylooligosaccharides, is also contained in wood, and xylan in these woods is also extracted to produce xylooligosaccharides. In addition, a method for producing xylo-oligosaccharides has been proposed in which xylooligosaccharides are produced from a reaction filtrate obtained from a hemicellulase treatment step of pulp (see, for example, Patent Document 1).

On the other hand, although corncob meal obtained by dry-crushing corncob is used as a fungal bed for mushroom cultivation, as an abrasive material for beans, as a nesting material for animals, etc., it is rarely used as an industrial raw material, and corncob produced is very small. Most of the waste is discarded as waste, and there are many problems such as the environmental degradation because the disposal method is mainly incineration, and the effective use of corncob is also being considered.

[0006]

[Patent Document 1] Japanese Patent Laid-Open No. 2000-333692

[0007]

SUMMARY OF THE INVENTION Therefore, the present invention has been made to solve the above problems, and is a process for producing xylo-oligosaccharide useful as a sweetener, which is made of corn cob meal which has been conventionally discarded as a raw material. The purpose is to provide a method.

[0008]

The above-mentioned object has been achieved by the present invention described below. That is, in the present invention, <1> corn cob meal is pressure sealed in the cylinder.
After being steamed with the multi-stage continuous extruder,
Xylanases were added to the filtrate from which solids had been removed by
A method for producing a xylo-oligosaccharide for decomposing, comprising:
The steaming conditions for each extruder in a multi-stage continuous extruder are
As you progress from the first extruder to the last, the temperature and
And the pressure rises, and the conditions for steaming the last extruder
Is 150 to 250 ° C. and 20 to 29 MPa, which is a method for producing a xylooligosaccharide. <2> Sulfite conversion during steaming of the corncob meal
Xylo according to <1>, characterized in that a compound is added.
It is a method for producing an oligosaccharide.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the method for producing a xylooligosaccharide of the present invention, first, a cylinder is pressure-sealed into a plurality of stages.
Steaming is performed with a continuous extruder (hereinafter, sometimes referred to as "steaming treatment"). In the present invention, the continuous pressing of
The cooking conditions for each extruder at the start are as follows:
Temperature and pressure increase as you progress to the last extruder
And the steaming condition of the last extruder is 150-
Characterized by 250 ° C. and 20-29 MPa
It

The steaming treatment in the present invention will be described. The steaming treatment in the present invention is carried out by adding water to corn cob meal (corn corn dried and crushed and powdered),
The last in the extruder, subcritical a (supercritical verge) state 150 to 250 ° C. and 20～29MP a
It steaming boiled.

The above-mentioned steaming treatment is performed in the last extruder.
Te is the temperature at 150~250 ℃, 20~29M pressure
It is necessary to set Pa, the temperature is preferably set to 180 to 200 ° C., and the pressure is preferably set to 25 to 28 MPa. The amount of water added is 100 parts by weight of corn cob meal.
10 to 1000 parts by mass is preferable, and 50 to 100 parts by mass is more preferable. The steaming treatment is preferably performed for 10 to 30 minutes, more preferably 15 to 20 minutes.

Further, in the steaming treatment, a sulfite compound can be added to corn cob meal together with water. By adding the sulfite compound to corncob meal,
The steaming treatment time can be shortened. Examples of the sulfite compound include sodium sulfite and calcium sulfite. Further, the addition amount of the sulfite compound is preferably 1 to 10 parts by mass, and more preferably 2 to 5 parts by mass with respect to 100 parts by mass of corn cob meal.

[0013] connecting the extruder the steaming process of the cylinder <br/> over as shown in FIG. 1 is pressure sealed, series two or more
It is preferably carried out by a continuous extruder having a plurality of stages connected .
FIG. 1 is a partial sectional view of an extruder in which the inside of a cylinder used for steaming treatment in the present invention is pressure-sealed. The extruder comprises a cylinder 1 having a material inlet 2 at its base,
A screw 3 having a spiral flight 4 for kneading (steaming) corncob meal and water (hereinafter sometimes simply referred to as “material”) charged from the material charging port 2 and extruding to the tip side, and a cylinder 1 Heater 5
And a driving means 6 such as a motor 7 connected to a power source (not shown) for rotating the screw 3 and a reduction gear 8 having a driving gear 9 and a driven gear 10, and the steamed and extruded product is taken out. The outlet 11 and the heat insulating and heat insulating material 12 for covering the cylinder 1 and the heater 5 are provided. In addition,
A pump (not shown) is attached to the material input port 2,
The material is fed into the cylinder 1 from the pump through the material inlet 2. Also, a spiral flight 4 of the screw 3, the pitch as rather closer to the outlet 11 is shortened. Further, a temperature sensor 13 and a pressure sensor 14 are arranged near the tip of the screw 3 of the cylinder 1.

The steaming treatment by the extruder shown in FIG. 1 is carried out by the following procedure. A material is charged into the cylinder 1 through a material charging port 2 by a pump (not shown), and the temperature inside the cylinder 1 is adjusted to a target temperature by the heater 5. Further, by rotating the rotation shaft of the motor 7 clockwise as viewed from the side of the motor 7, the driving gear 9 is rotated clockwise, the driven gear 10 is rotated counterclockwise, and the screw 3 is further rotated counterclockwise. The corn cob meal is extruded in the direction of the outlet 11 while steaming. Spiral flight 4 of the screw 3, the pitch as rather closer to the outlet 11 is shorter, corncob meal outlet 11
Predetermined pressure is compressed as the rather closer is applied to. The corn cob meal after the steaming treatment is extruded from the outlet 11. In this embodiment, the temperature sensor 13 and the pressure sensor 14 are arranged near the tip of the screw 3 of the cylinder 1. However, the temperature sensor 13 is arranged at the tip of the screw 3 from the axial center of the cylinder 1. The position of the pressure sensor 14 may be the cylinder 1
It suffices if the space is ¼ of the front end side of the screw 3.

The steaming treatment according to the extruder according to Figure 1, the outermost
In the later extruder, the temperature and pressure measured by the temperature sensor 13 and the pressure sensor 14 are 150 to 250 ° C.,
Pressure: It is necessary to satisfy the condition of 20 to 29 MPa.

The steaming treatment in the present invention is a method in which two or more extruders shown in FIG. 1 are connected in series, that is, the steaming treatment is carried out by the first extruder (first extruder) and the outlet 1
It carried out by a method of further cooked input mixture directly to the second extruder of the material inlet 2 and extruded corn cob meal and water from 1. When two or more extruders shown in FIG. 1 are connected in series and steamed, the steaming conditions for each extruder are:
As the cooking conditions of the last connected extruder (last extruder) satisfy the conditions of temperature: 150 to 250 ° C., pressure: 20 to 29 MPa, and as the first extruder progresses to the last connected extruder, temperature and pressure that has risen.

[0017] The steaming treatment of the above corncob meal, and and cellulose (produced by changing the lignin) lignocellulose polyphenols produced by decomposition of soluble Hemise Le loin (hereinafter, referred to as "soluble xylan.") And Is obtained, and a mixed solution of polyphenol and soluble xylan is obtained as a filtrate by filtering (filtering) this with a filtering device.

The soluble xylan obtained by the filtration treatment becomes a xylooligosaccharide by being subjected to a hydrolysis treatment (enzyme treatment) with xylanase. The enzyme treatment can be performed by the following procedure. In a reaction vessel equipped with a stirrer and having a heat retaining mechanism, xylanase is added to the filtrate from which solids have been removed by a filter to cause a reaction, and suspended substances are removed from the resulting reaction product by a filter and dried. .
Xylooligosaccharide is obtained by the above procedure.

The amount of xylanase added in the enzyme treatment is preferably 0.1 to 5 parts by mass, more preferably 0.5 to 2 parts by mass, relative to 100 parts by mass of the filtrate. In addition, the enzyme treatment is preferably carried out under the conditions of pH 3 to 8,
It is more preferable to carry out under the conditions of 4 to 6. Further, the treatment temperature is preferably 30 to 50 ° C, more preferably 40 to 45 ° C. The stirring speed is preferably 60 to 200 rpm,
More preferably, it is 100 to 150 rotations. Processing time is 15 ~
30 hours are preferable and 20 to 24 hours are more preferable.

Xylooligosaccharides are used in various foods because they have the effects of preventing tooth decay and balancing intestinal bacteria to promote health, and it is expected that demand will greatly increase in the future.

[0021]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. (Example 1) Steaming of corncob meal was carried out by using four extruders in series, which were similar to the pressure-sealed extruder shown in FIG. The four extruders connected in series are connected to the outlet 11 of the first extruder and the material inlet 2 of the second extruder, and are similarly connected to the fourth extruder. The kneaded material cooked by the first extruder is directly charged into the cylinder 1 through the material input port 2 of the second extruder, and similarly reaches the material input port 2 of the fourth extruder.

To 100 parts by mass of corncob meal, 5 parts by mass of calcium sulfite and 50 parts by mass of water were added, and the mixture was charged into the cylinder 1 through the material charging port 2 of the pressure-sealed extruder shown in FIG. Next, the temperature and pressure of the first extruder were set to the values shown in Table 1, the motor was driven to rotate the screw 3 to knead (steam) for 5 minutes, and then the outlet 11
Extrude from. The kneaded product extruded from the outlet 11 was directly introduced into the cylinder 1 through the material introduction port 2 of the second extruder, and similarly kneaded (steamed) up to the fourth extruder. The setting conditions and kneading (steaming) time of each extruder are as shown in Table 1. The temperatures and pressures shown in Table 1 are
It is a value measured by the temperature sensor 13 and the pressure sensor 14.

[0023]

[Table 1]

The filtrate obtained by filtering the steamed corn cob meal with a filtration device was placed in a reaction vessel having a heat retaining mechanism equipped with a stirring device, and the filtrate 1
After adding 3 parts by mass of xylanase and 0.1 parts by mass of sodium hydroxide to 00 parts by mass in the reaction vessel,
The reaction was carried out at a temperature of 45 ° C. and a stirring speed of 150 rpm for 24 hours to obtain a xylooligosaccharide.

[0025]

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a method for producing xylo-oligosaccharide useful as a sweetener, which is made of corncob meal which has been conventionally discarded.

[Brief description of drawings]

FIG. 1 is a partial cross-sectional view of an extruder in which a cylinder is pressure-sealed, which is an example of a pressure vessel for performing a steaming process in the present invention.

[Explanation of symbols] 1 cylinder 2 Material input port 3 screws 4 spiral flight 5 heater 6 Drive means 7 motor 8 reduction gears 9 Driving gear 10 Driven gear 11 Exit 12 Thermal insulation 13 Temperature sensor 14 Pressure sensor

─────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP 2000-333692 (JP, A) JP 2001-205070 (JP, A) JP 11-113600 (JP, A) JP 2002-59118 (JP , A) JP 2001-262162 (JP, A) JP 54-14530 (JP, A) JP 49-26640 (JP, B1) (58) Fields investigated (Int.Cl. ⁷ , DB name) ) C12P 19/00-19/64 C13K 13/00 B01J 3/00 B09B 5/00 CA / BIOSIS / WPIDS (ST N) JISST file (JOIS)

Claims (2)

(57) [Claims] 1. The corncob meal is compressed in the cylinder.
Steamed in a tightly closed multi-stage continuous extruder and then filtered
Xylanase was added to the filtrate from which solids were removed by the device,
A method for producing a xylooligosaccharide to be hydrolyzed, wherein the steaming conditions of each extruder in the multistage continuous extruder are set.
However, as I progressed from the first extruder to the last extruder,
The temperature and pressure rise, and the steaming conditions of the last extruder are 150 to 25
It is 0 degreeC and 20-29 MPa . The manufacturing method of the xylooligosaccharide characterized by the above-mentioned. 2. When steaming the corn cob meal,
The method according to claim 1, wherein a sulfite compound is added.
A method for producing the above-mentioned xylooligosaccharide.