JP3397258B2 - Calcium absorption promoting water-soluble fraction, composition containing the same, and calcium absorption promoting additive - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to walleye pollock or oki.
A calcium-absorption-promoting, water-soluble fraction obtained by treating a protein derived from Ami with pepsin, a calcium-absorption-promoting composition containing the same, and a calcium-absorption-promoting composition comprising the same, which is used in food, feed or pharmaceuticals It relates to additives.

[0002]

2. Description of the Related Art Looking at the results of the National Nutrition Survey of 1991 published by the Ministry of Health and Welfare, the average calcium intake of Japanese people is 540 mg on average per day, which is well below the capacity of 600 mg. There is. In recent years, it has become known that insufficient intake of calcium causes serious diseases such as osteoporosis, hypertension, and colon cancer. Especially for osteoporosis, it is highly probable that the elderly will become bedridden when they have a femoral neck fracture or lumbar compression fracture, and the second cause of becoming an elderly bedridden is due to the fracture. is there. It is reported that the number of patients with osteoporosis is 5 million, and it is said that the number of patients will increase and become a major social problem in the future toward an elderly society.

The first requirement for improving such a situation is to increase the intake of calcium and to constantly supply sufficient calcium to the body. However, the daily intake of calcium of Japanese people has never exceeded the capacity (600 milligrams) in the past according to the statistics surveyed by the Ministry of Health and Welfare every year. Is difficult. In addition, medicines and dietary supplements containing calcium have problems in terms of taste and are difficult to take at all times. Based on this situation, we thought that it is important that orally ingested calcium be efficiently absorbed in the intestinal tract, which is the absorption site. That is, the ratio of orally ingested calcium that is absorbed in the intestinal tract and used for the maintenance of the living body is about 20 to 50%, although there is some difference depending on the origin of calcium. Most are thought to be excreted outside the body without being absorbed. In fact, if the absorption rate of calcium in the intestinal tract increases, a sufficient amount of calcium will be utilized by the living body without excessive intake of calcium.

Vitamin D and lactose are generally used in the fields of foods and pharmaceuticals as components for promoting absorption of calcium, but since vitamin D is synthesized in the body, sufficient vitamin D in the body is obtained. The effect cannot be expected so much for the person who is synthesized. In addition, lactose is not preferable for people who are intolerant to lactose because of side effects such as diarrhea. In addition to the above, as a component that promotes absorption of calcium, a peptide derived from bone (Japanese Patent Laid-Open No. 4-16165)
Japanese Patent Application Laid-Open No. 4-10), butyric acid as a basic component
No. 8360) and galacto-oligosaccharides (Japanese Patent Laid-Open No. 4-134031), but they have not been put into practical use due to problems in production and utilization.

[0005]

DISCLOSURE OF THE INVENTION The present invention relates to a calcium absorption promoting water-soluble fraction effective as a component for promoting absorption of calcium in the intestinal tract, a calcium absorption promoting composition containing the same, a food and a feed. Alternatively, it is intended to provide an additive for promoting calcium absorption for use in medicine. Therefore, the present invention aims to provide a food, a feed or a drug having a calcium absorption promoting function at a low cost by a simple production method.

[0006]

It has been conventionally known that simultaneous ingestion of calcium and other food ingredients simultaneously results in a slightly higher absorption rate than ingestion of calcium alone. This phenomenon is explained that when food enters the stomach, the secretion of gastric acid is activated, the pH in the stomach is lowered, and calcium, which is generally insoluble in water, is dissolved by the acid, which facilitates absorption. However, the effect of protein components and peptides obtained by enzymatically degrading them on the absorption of calcium has not been sufficiently studied. As a result of research on the effect of enzymatic degradation of several protein components derived from various raw materials such as meat and plants on calcium absorption in the intestinal tract, especially skeleton
Treat proteins from cod or krill with pepsin
It was discovered that the obtained component has a high calcium absorption promoting effect, and the present invention was completed.

[0007] The gist of the present invention is the walleye pollock or okia
The purpose is to obtain a component obtained by treating pomegranate-derived protein with pepsin .

When enzymatically degrading proteins derived from walleye pollack or krill, proteolytic enzymes derived from animals and plants such as trypsin, chymotrypsin, papain and microorganisms are generally used for food processing and seasoning production.
Is also available. However, when it is decomposed with a general endo-type proteolytic enzyme, it is decomposed by gastric acid and digestive enzymes in the stomach after ingestion, so when it reaches the intestinal tract, which is a site of calcium absorption, It is expected that the structure will be different. Therefore, it is particularly preferable to use pepsin, which is a digestive enzyme in the stomach, as the proteolytic enzyme used.

When degrading a marine product-derived protein with a proteolytic enzyme, it is desirable to heat the protein to 80 ° C. or higher before or after suspending it in water to inactivate the endogenous enzyme. Subsequent enzymatic reactions are performed using an optimal p-value of the enzyme using an aqueous suspension or solution of protein.
It is carried out by adjusting the temperature to H or below the optimum temperature, adding an enzyme, and leaving it for several tens of minutes to several hours or stirring. The amount of enzyme added is usually 1/10 to 1/100 of the substance to be decomposed.
It is about 00. When pepsin is used as a proteolytic enzyme, the optimum pH is 1.5 to 2.5, so a protein solution or suspension should be treated with a pH adjusting agent such as hydrochloric acid, sulfuric acid, acetic acid, or citric acid. It is necessary to adjust the pH in this range in advance. The optimum reaction temperature of pepsin is 45 to 60 ° C., but there is no particular problem as long as it is below this temperature.

In order to stop the oxygen reaction carried out in this manner, (1) a method of inactivating the proteolytic enzyme by heating it to 80 ° C. or higher, (2) adding an inhibitor of the proteolytic enzyme The method, (3) the method of adjusting the pH of the reaction solution within the optimum range of the enzyme is generally used. It is possible to stop the reaction of pepsin with an optimum pH on the acidic side by the method of (3), but in order to completely inactivate other contaminating proteolytic enzymes, (1) It is preferable to use the above method. After the oxygen decomposition, heating at 100 ° C. did not affect the function of promoting the calcium absorption of the decomposition product.

The protein degradation product obtained by the above method is divided into water-soluble components and insoluble components.
Since the component that promotes calcium absorption is contained in the water-soluble fraction, it is preferable to remove the insoluble fraction by centrifugation, ultrafiltration or the like when it is desired to obtain the effect by adding a small amount. Further, after obtaining the water-soluble fraction, it may be dried and powdered depending on the purpose of use. The food, feed or drug having a calcium absorption promoting function of the present invention contains these water-soluble fractions and calcium salts. Examples of foods include beverages, confectionery, cereals, noodles, paste products, and tablets. For the feed, it is used as a mixture with normal feed. In medicine, it can be used in the form of tablets, granules or liquid.

[0012]

The details of the present invention will be described with reference to examples. The invention is in no way limited by these examples.

Example 1 A calcium absorption promoting component derived from aquatic protein was prepared as follows. Frozen walleye pollack 100 g (nitrogen-containing fraction 15 g) was thawed, 1 kg of water was added, and the mixture was suspended with a homogenizer and heated at 100 ° C. for 10 minutes to inactivate coexisting enzymes. Next, the pH of the suspension was adjusted to 1.8 with hydrochloric acid, and 10 g of pepsin (1: 60000 manufactured by SIGMA) was added.
The mixture was added and stirred at 40 ° C. for 4 hours while keeping the temperature. After that, 100 ℃
The mixture was heated at 20 ° C. for 20 minutes to deactivate pepsin, and sodium hydroxide was added to adjust the pH to 6.5. Then centrifuge (4
(000 × g, 10 minutes) was performed to obtain a water-soluble supernatant fraction. The water-soluble nitrogen-containing fraction was 2.3 g.

Example 2 Calcium absorption promoting effect of a marine product-derived protein component was tested. (1) Preparation of test solution The defatted soybean protein, chicken protein and cod derived from marine products and Antarctic krill protein were decomposed with pepsin by the method of Example 1 to obtain a water-soluble fraction, 1.2 mg each.
It was dissolved in water so that the concentration became / ml. Then, calcium chloride is dissolved to a calcium concentration of 150 mM, and the pH is adjusted to 6.5 with hydrochloric acid or sodium hydroxide.
Adjusted to. As a control, a 150 mM calcium chloride solution alone or a solution containing 15% lactose having a calcium absorption promoting effect was used.

(2) Experimental Animal As an animal, a 4-week-old male SD rat (manufactured by Charles River Japan) was used after fasting overnight. The number of animals is 1
6 groups

(3) Calcium Absorption Test The calcium absorption test was carried out by the double ligation method of the intestinal tract of rats. That is, a rat was subjected to laparotomy under Nembutal anesthesia and a 4 cm length of the duodenum was ligated with a thread to prepare a sausage loop. Then, 0.3 ml of the test solution was injected into the lumen of the duodenum with an injection needle. The rat was allowed to stand for 1 hour while being kept at about 37 ° C. so as not to lower the body temperature, and then the duodenum was cut out, and the amount of calcium remaining in the intestinal tract was measured by an atomic absorption measuring device.
The absorption rate of calcium (Ca) was calculated by the following formula.

(4) Test Results The test results are shown in FIG. As shown in the figure, a very high calcium absorption rate was obtained in the group to which cod and the pepsin decomposition product of Antarctic krill were added, as compared with the control group containing only calcium. The defatted soybean protein and chicken meat protein, which were used for comparison, had higher absorption rates than the calcium-only group, but were inferior to the marine-derived protein.

Example 3 A beverage containing a calcium absorption promoting component was produced. Each component of the beverage was mixed and dissolved according to the formulation shown in Table 1 below, and the mixture was sterilized by heating at 90 ° C for 40 minutes to obtain a calcium beverage.

[0019]

[Table 1]

Example 4 An enteral nutritional supplement containing a calcium absorption promoting component was produced. The ingredients shown in Table 2 below were mixed and emulsified to prepare a nutritional beverage excellent in calcium absorption.

[0021]

[Table 2]

Example 5 A lemon jelly containing a calcium absorption promoting component was prepared.
15 g of powdered gelatin and 120 g of sugar were added to and dissolved in 475 g of warm water. Next, lemon juice 20g, lemon peel 10g, white curacao 3g, calcium chloride 3
g and 1 g of a decomposed product of cod, the mixture was stirred and put in a jelly mold. The mixture was cooled in a refrigerator for 3 hours to obtain lemon jelly containing a calcium absorption promoting component.

Example 6 A fish sausage containing a calcium absorption promoting component was prepared. 35 g of salt was added to 1150 g of Alaska pollack surimi and salted, and 430 g of ice water, 30 g of sugar, 85 g of potato starch, 20 g of skim milk powder, 20 g of seasoning, soybean oil 2
Add 60 g, pigment 2 g, krill pepsin decomposition product 15 g, calcium carbonate 5 g, crush with a crusher, stuff in a casing, seal, and treat with a retort sterilizer at 118 ° C for 20 minutes, calcium absorption promoting component calcium I got fortified fish sausage.

[0024]

EFFECTS OF THE INVENTION It is possible to provide a calcium absorption promoting water-soluble fraction, a calcium absorption promoting composition containing the same, and a calcium absorption promoting additive for use in foods, feeds or pharmaceuticals. For all people, including old people, infants, and mammals who lack calcium intake,
A component that efficiently increases the amount of calcium absorbed in the intestinal tract and foods, feeds, and pharmaceuticals containing the same can be provided at low cost.

[Brief description of drawings]

FIG. 1 is a drawing showing the effect of pepsin degradation products of cod and Antarctic krill on calcium absorption.

Front page continuation (51) Int.Cl. ⁷ Identification code FI A61K 38/00 A61P 3/02 A61P 3/02 A23L 1/304 // A23L 1/304 A61K 37/18 (72) Inventor Ikuo Kimura Hachioji City 205-1 Naganuma-cho Grand Yuki 203 (56) References JP-A-2-273144 (JP, A) JP-A-60-221040 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB) Name) A23J 3/04 A23J 3/34 A23L 1/30-1/305 A23K 1/10 A23K 1/16 JISC file (JOIS)

Claims (7)

(57) [Claims] 1. A calcium absorption-promoting water-soluble fraction obtained by treating a protein derived from walleye pollack or krill with pepsin . 2. A calcium absorption promoting composition comprising the water-soluble fraction according to claim 1 as an active ingredient. 3. The calcium absorption promoting composition according to claim 2, which further contains a calcium salt as an active ingredient. 4. An additive for promoting calcium absorption, which comprises the composition for promoting calcium absorption according to claim 2 or 3. 5. The additive for promoting calcium absorption according to claim 4, which is used in foods. 6. The additive for promoting calcium absorption according to claim 4, which is used for feed. 7. The additive for promoting calcium absorption according to claim 4, which is used in medicine.