JP3393559B2 - Skin preparation - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin external preparation effective for preventing and improving aging symptoms such as wrinkles, spots, roughness of texture, roughness and roughness of skin, and improving rough skin symptoms.

[0002]

2. Description of the Related Art Conventionally, many studies have been conducted on external preparations for the skin to prevent or improve aging symptoms such as wrinkles, spots, roughness of the skin, roughness, and roughness of the skin, and to improve rough skin symptoms. Came. Recently, in the field of external preparations for skin, attempts have been made to activate skin cells themselves and activate the functions of the skin itself to improve skin symptoms and prevent aging.

As such attempts, skin cell activators have been sought for hormones, various vitamins, herbal medicine extracts, animal organ extracts, plant extracts and the like. Among them, cosmetics and the like in which collagen or elastin, which is a constituent protein of connective tissue, are added, and a soluble eggshell membrane is used to activate fibroblasts have been proposed (Japanese Patent Publication No. 60-197).
25, JP-A-48-40943, JP-A-3-19080.
8). In addition, we have found that α-hydroxy acids such as glycolic acid and lactic acid, or their salts, or derivatives such as sterol esters promote the proliferation of fibroblasts,
It was found to be effective in preventing and improving skin aging,
Japanese Patent Application Laid-Open No. 5-112422, Japanese Patent Application No. 5-1
57843).

[0004]

However, in the above skin cell activating agent, there is a limitation in use due to the problem of side effects, the action and effect are not sufficient, and a considerably large amount must be blended. There were problems such as. In addition, it is particularly difficult to control the quality of substances derived from plants or animals, and it is difficult to receive a stable skin activating agent. Furthermore, when formulated as a skin external preparation with other ingredients, the activity is Some were lost. In particular, since collagen, elastin, soluble eggshell membranes, etc. consist of proteins, there is a problem in terms of sensitization. With respect to the α-hydroxy acids, too, when the compounding amount is increased, skin irritation may appear, and due to problems such as skin absorption, the expected effects may not be obtained.

[0005]

[Means for Solving the Problems] In order to solve the above-mentioned problems and to provide an external preparation for the skin free of adverse effects such as side effects and irritation to the skin, etc., it has been found to have a fibroblast activating effect. Glycolic acid, its salt
Lumin and its derivatives (except salts) and 1 of its derivatives
By using the seeds and above together with the protein extracted from the eggshell membrane, synergistic promotion of fibroblast proliferation was observed with a small amount of each.

As the α-hydroxy acid, glycol is used.
Acids, their salts (salts with ethanolamine and its derivatives
Except), and derivatives such as esters with sterols can also be used.

In the present invention, a film attached to the inside of the eggshell of birds such as chicken eggs and quail eggs is used as the eggshell membrane,
Proteins that are solubilized and extracted with acid agents, alkaline agents, organic solvents, etc. are used.

[0008] The effective amount of each component can be exerted in a much smaller amount than when used alone. Glycolic acid, its salt (ethanolamine
And salts thereof and its derivatives are 1
0-6th to 10% by weight, egg shell membrane extracted protein
It is suitable that the compounding amount of 0.00001 to 10% by weight.
It In addition, other components that are usually used in external preparations for skin can be used in combination, and the external preparations may take the form of lotion, skin emulsion, skin cream, ointment and the like. It can be provided as a cosmetic.

[0009]

When the external preparation for skin according to the present invention is applied, the dermal line
The growth promoting action of fibroblasts is synergistically enhanced, resulting in wrinkles and wrinkles.
Loss of texture, roughness of texture, improvement of roughness and roughness, etc.
Excellent prevention and improvement of skin aging symptoms, improvement of rough skin symptoms
Goodness is recognized.

[0010]

EXAMPLES Further, the features of the present invention will be described in detail with reference to Examples. First, as a beauty lotion according to the present invention, the formulation of Example 1 and the formulations of Comparative Examples 1 and 2 are shown in Table 1.
Shown in. In Table 1, the components (1) to (9) are mixed with (11) and dissolved to homogenize, and after adding (10), the total amount is 100% by weight in (11).

[0011]

[Table 1]

With respect to the above Examples and Comparative Examples, the effect of improving skin aging symptoms and the effect of healing rough skin symptoms were examined. First, for the improvement of skin aging symptoms, for each sample, 20 male and female panelists in their 50s to 60s, in which such symptoms were noticeably used, were blindly used for 1 month, and the skin condition was changed before and after use. Was observed and evaluated.
As an index of skin aging symptoms, four items of skin wrinkles, spots, texture, and acupuncture were selected, and wrinkles, spots, and texture were observed by taking photographs and replicas of the skin surface, and the improvement status was evaluated. The skin elasticity was evaluated by measuring the skin elasticity. Improvements situation, "A;improvement","B: slightly improved"; and evaluated in three levels: "C no change", Example
The panel which obtained each evaluation in Table 2 about 1 and Comparative Examples 1 and 2.
It is shown by the Ra number.

[0013]

[Table 2]

In Table 2, Example 1 in which glycolic acid, which is one of α-hydroxy acids, and egg shell membrane-extracted protein are used in combination is markedly different from that in Comparative Example 1 or 2 in which they are mixed alone. It has been shown to improve wrinkles, texture and acupuncture. Regarding the skin stains, an improvement tendency was observed in 85% of the panelists, and a clear improvement was observed in 7 persons. In Example 1, such a remarkable synergistic effect was observed by only adding 0.2% by weight of glycolic acid and the protein extracted from eggshell membrane.

Next, regarding the healing effect of the rough skin condition, the dorsal skin of the mouse was treated with an aqueous solution of 5% by weight of sodium lauryl sulfate to artificially induce the scale-like rough condition, and 1 sample of each sample was added thereto. Apply twice a day for 5 consecutive days,
The healing situation was observed and evaluated. Ten mice were used for each sample, and the healing status of the rough symptom was evaluated in three grades of "complete cure", "moderate cure", and "no change", and the number of mice obtained each evaluation is shown in Table 3 . Indicated.

[0016]

[Table 3]

In Table 3, glycolic acid and eggshell membrane extraction
In Example 1 in which the protein produced is used in combination,
A complete cure was observed in the suture. In contrast, each of the above components
Some healing was observed in each comparative example in which
At best, only half of the mice showed good healing
In some cases, it was not possible.

[0018]

As described above, in the present invention, glyco
Acid, its salt (salt with ethanolamine and its derivatives
, Except) and derivatives together with egg shell membrane extracted protein
As a result, it is possible to add a very small amount of each, and
Synergistic effect of skin aging and rough skin compared to when used
A significant improvement effect was observed. Therefore, it was possible to provide an extremely safe and highly effective external preparation for skin for preventing aging and improving skin symptoms, which is free from skin irritation and skin sensitization.

─────────────────────────────────────────────────── --Continued from the front page (56) References JP-A-5-163132 (JP, A) JP-A-5-112422 (JP, A) JP-A-3-190808 (JP, A) JP-A-48- 40943 (JP, A) JP 48-40968 (JP, A) JP 60-214719 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00-7 / 50 CA (STN)

Claims (2)

(57) [Claims] 1. Glycolic acid, its salt (ethanolami)
And a derivative thereof, and at least one selected from the group consisting of derivatives, and a protein obtained by extracting from eggshell membranes. 2. The external preparation for skin according to claim 1, wherein the external preparation for skin is a cosmetic.