JP3233476U - Sheet to be placed on the microplate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a sheet on which characters, symbols and the like can be easily written around a well on an upper surface of a microplate. SOLUTION: A sheet 10 is used by being placed on an upper surface of a microplate provided with a plurality of wells, and one side of the sheet 10 can be written by a writing tool or printed by a printing machine. The surface 11 is provided with holes 12 at positions corresponding to each of the plurality of wells. [Selection diagram] Fig. 1

Description

The present invention relates to a sheet that is used by being placed on a microplate.

A microplate (also referred to as a "microtiter plate") is an experimental / inspection instrument in which a large number of wells (dents) for accommodating samples such as microorganisms and cultured cells are formed on a flat plate (see, for example, Patent Document 1). By accommodating different samples in each well, mounting a microplate on the measuring device, and measuring the samples in each well one after another or at the same time, it is possible to perform measurement on a large number of samples automatically or at high speed. can. Examples of the measurement performed by the measuring device include detecting the fluorescence generated from the sample by irradiating the sample and measuring the wavelength thereof, measuring the absorbance of the light irradiated to the sample, and the like.

Japanese Unexamined Patent Publication No. 2020-190522

When performing a measurement using such a microplate, the measurer may write characters, symbols, etc. around the well on the upper surface of the microplate with a ballpoint pen or the like. For example, information for specifying the sample such as the name of the sample contained in each well and preparation conditions, and a symbol for distinguishing the well containing the specific sample from other wells are described. However, when the sample is contained in the well, the sample may spill out from the well due to the finger touching the sample in the well or the vibration generated at the time of writing, and it is not possible to easily write characters and symbols. ..

The problem to be solved by the present invention is to make it possible to easily write characters, symbols, etc. around the well on the upper surface of the microplate.

The present invention made to solve the above problems is a sheet used by placing it on the upper surface of a microplate provided with a plurality of wells, and one surface of the sheet is filled in or printed with a writing tool. It is a writing surface that can be printed by a machine, and is a sheet in which holes are provided at positions corresponding to each of the plurality of wells.

The sheet according to the present invention is used by placing it on the upper surface of the microplate by aligning the positions of each of the plurality of wells and the holes corresponding thereto. Before placing the sheet on the micro plate in this way, write characters and symbols on the writing surface with a writing tool (for example, a ball pen, sign pen, etc.), or write characters and symbols with a printing machine (for example, an inkjet printer, a laser printer, etc.). By printing, etc., characters, symbols, etc. can be easily written without affecting the sample contained in the well.

The shape of the hole may be the same as the planar shape of the well, or may be different from the planar shape of the well. In particular, the shape of the hole should be smaller than the planar shape of the well in that writing can be facilitated and the amount of information that can be written can be increased by widening the portion where writing can be performed. Is preferable.

In the sheet according to the present invention, it is preferable that an adhesive adhering to the upper surface of the microplate is applied to the surface (back surface) opposite to the description surface. As a result, the sheet can be fixed to the upper surface of the microplate, and the position of the sheet can be prevented from shifting. It is preferable to attach a release sheet to the adhesive-coated surface.

Alternatively, the back surface may be a description surface (second description surface) capable of writing with a writing tool or printing with a printing machine, similarly to the description surface. As a result, the amount of information that can be described on the sheet can be increased. The information described on the second description surface cannot be visually observed when the sheet is placed on the upper surface of the microplate, but the described information can be saved by collecting the sheet after the measurement is completed. The second description surface can be utilized for the purpose of doing so.

Many microplates are rectangular in shape, with notches in one of their four corners or two adjacent corners to identify their orientation and uniquely locate each well. ing. Therefore, the sheet according to the present invention is also provided with notches in one of the four corners of the rectangle or two adjacent corners corresponding to them, and the sheet is microplated in a state where the positions of the notches are aligned. By placing it on the upper surface of the well, the correspondence between the well and the hole (and the description in the vicinity thereof) can always be correct.

In the sheet according to the present invention, further, a symbol for identifying each of the wells corresponding to each of the holes is described at a position in the vicinity of each of the holes on the description surface. Can be done. A sheet on which such an identification symbol is written is placed on the upper surface of the microplate, the surface thereof is photographed with a camera, and the identification symbol is recognized by a computer, so that information (of the sample) for each well can be obtained. It can help you manage it on your computer. For example, a number, an alphabet, or the like can be used as the identification symbol. Further, a bar code, a two-dimensional code (QR code: registered trademark) or the like may be used as the identification symbol.

According to the present invention, characters, symbols, etc. can be easily written around the well on the upper surface of the microplate.

Top view (a) and bottom view (b) showing an embodiment of a sheet to be placed on a microplate according to the present invention. Top view (a) and perspective view (b) showing an example of a microplate on which the sheet of the present embodiment is placed on the top surface. The top view which shows the modification of the sheet of this embodiment. Top view showing a state in which characters and symbols are written on the description surface of the sheet of this embodiment. The perspective view which shows the state during the operation which puts the sheet of this embodiment on the upper surface of a microplate. The top view which shows the state which put the sheet of this embodiment on the upper surface of a microplate. The top view which shows the example which the symbol was printed on the description surface of the sheet of this embodiment. The top view which shows the example which printed the bar code on the description surface of the sheet of this embodiment. Top view (a) and bottom view (b) showing a sheet having a second description surface, which is another embodiment of the present invention. Top view showing a plurality of examples in which the number of holes is different in the sheet of this embodiment.

An embodiment of a sheet to be placed on a microplate according to the present invention will be described with reference to FIGS. 1 to 10.

FIG. 1 (a) of the sheet 10 of the present embodiment is shown in a top view, and FIG. 1 (b) is shown in a bottom view. This sheet 10 is used by being placed on the upper surface 91 of the microplate 90 shown in FIG.

The sheet 10 is made of paper and has a rectangular shape as a whole. The front surface of the sheet 10 is a writing surface 11 capable of writing with a writing tool (for example, a ballpoint pen or felt-tip pen) or printing with a printing machine (for example, an inkjet printer or a laser printer). The adhesive 13 is applied to the back surface of the sheet 10. Hereinafter, this back surface may be referred to as an “adhesive-coated surface”. Before using the sheet 10, a release sheet (not shown) is attached to the adhesive-coated surface.

The sheet 10 is provided with a total of 96 circular holes 12 so that 8 circular holes 12 are arranged vertically and 12 circular holes 12 are arranged horizontally. The number and position of the holes 12 are determined according to the number and position of the wells 92 of the microplate 90. The size (diameter) of the hole 12 is preferably smaller than the size (diameter) of the planar shape of the well 92. For example, when the diameter of the circular circle having a planar shape of the well 92 is 7 mm, it is preferable that the sheet 10 is provided with a circular hole 12 having a diameter in the range of 4 to 7 mm. Here, when the size of the hole 12 is smaller than the planar shape of the well 92 (the diameter of the hole 12 is other than 7 mm), the description surface is larger than the portion of the upper surface 91 of the microplate 90 other than the well 92. 11 can be taken widely. Although the description surface 11 is narrow, it is included in the scope of the present invention even when the size of the hole 12 is larger than the planar shape of the well 92.

The shape of the microplate 90 is rectangular, and a notch 94 is provided in one of the four corners of the rectangle to specify its orientation (see FIG. 2). Correspondingly, the sheet 10 is also provided with a notch 14 at one of the four corners of the rectangle. The notch of the microplate 90 may be provided at two adjacent corners of the four corners of the rectangle. In that case, the sheet is also provided with notches 141 and 142 at two adjacent corners of the four rectangular corners (sheet 10A shown in FIG. 3).

A method of using the sheet 10 of the present embodiment will be described with reference to FIGS. 4 to 6. First, each of the wells 92 contains a sample. Then, on the writing surface 11 of the sheet 10, characters, symbols, etc. are written using a writing tool, or characters, symbols, etc. are printed using a printing machine. As such characters and symbols, for example, information for identifying a sample such as the name and preparation conditions of a sample contained in each of the wells 92, and for distinguishing a well 92 containing a specific sample from other wells 92. Examples include symbols that serve as landmarks. FIG. 4 shows an example in which the character 81 and the symbol 82 are described in the vicinity of the well 92.

Then, the release sheet attached to the adhesive-coated surface of the sheet 10 is peeled off from the sheet 10 so that the position of the notch 14 of the sheet 10 is aligned with the position of the notch 94 of the microplate 90 (FIG. 5). , The sheet 10 is attached to the upper surface 91 of the microplate 90 from the adhesive-coated surface side. As a result, the holes 12 of the sheet 10 are arranged on the upper surface of each well 92. Since the size of the hole 12 is smaller than the size of the planar shape of the well 92 (FIG. 6), the description surface 11 of the sheet 10 is wider than the portion of the upper surface 91 of the microplate 90 other than the well 92. Therefore, the amount of description on the description surface 11 can be increased.

After that, the microplate 90 is set in the measuring device, and a predetermined measurement is performed on the sample contained in each well 92. After the measurement is completed, the information described on the description surface 11 can be stored by peeling the sheet 10 from the upper surface 91 of the microplate 90 and collecting the sheet 10.

In the above example, the sheet 10 is attached to the upper surface 91 of the microplate 90 after the information is described on the description surface 11, but the information is described on the description surface 11 after the sheet 10 is attached to the upper surface 91 of the microplate 90. You may.

Further, in the above example, the executor of the measurement describes the information on the description surface 11 during a series of operations when performing the measurement, but even if the information is described on the description surface 11 before the start of the measurement operation. Often, in that case, the information may be described by a person other than the person who performed the measurement (for example, the manufacturer of the sheet 10).

In the example shown in FIG. 7, an identification symbol consisting of a character 83 for identifying each of the 96 wells 92 is printed in advance by the manufacturer of the sheet 10 in the vicinity of the hole 12 corresponding to each well 92 on the writing surface 11. (Has been described. In this example, eight rows in the vertical direction of the figure are labeled with the letters A to H, and 12 columns in the horizontal direction of the figure are numbered from 1 to 12, and each well 92 corresponds to it. By writing the alphabet of the row and the number of the column in succession such as "A1", it is used as a symbol for identifying each well 92. Instead, each well 92 may be identified by another method, such as assigning a serial number from 1 to 96 to the 96 wells 92. Further, in the example shown in FIG. 8, an identification symbol consisting of a barcode 85, which is an identification code for identifying the wells 92 corresponding to each of the holes 12, is printed (described) in the vicinity of each of the holes 12 of the description surface 11. ) Has been. A two-dimensional code may be printed (described) as an identification symbol instead of the barcode 85.

A sheet 10 on which identification symbols such as characters 83 and barcode 85 are described is placed on the upper surface 91 of the microplate 90, the upper surface 91 is photographed by a camera, and the identification symbols are recognized by a computer. , Sample information for each well 92 can be managed by a computer.

FIG. 9 shows a sheet 10B which is another embodiment of the present invention. The back surface of this sheet 10B is not coated with an adhesive, and the back surface is a writing surface (second writing surface 11B) that can be written with a writing tool or printed with a printing machine. Except for these points, the sheet 10B has the same configuration as the above-mentioned sheet 10.

The sheet 10B is used by being placed on the upper surface 91 of the microplate 90 after writing or printing characters, symbols, etc. on the description surface 11 or the second description surface 11B. In this way, since information can be described on both the description surface 11 and the second description surface 11B on the sheet 10B, the amount of information that can be described can be increased. By collecting the sheet 10B after the measurement is completed, the information described on the description surface 11 and the second description surface 11B can be saved.

The present invention is not limited to the above-described embodiment and modifications, and various modifications can be made within the scope of the present invention. For example, the shape and size of the holes provided in the sheet are circular in the range of 4 to 7 mm in diameter in each of the above examples, but the diameter may be changed according to the planar shape of the wells of the microplate. If the planar shape of the well is other than circular, the shape of the hole may be other than circular (for example, square) accordingly. Further, in each of the above examples, the size of the hole is made smaller than the planar shape of the well, but it may be the same size as the planar shape of the well or may be larger than that. The number of holes provided in the sheet is not limited to 96 in the above example, and may be appropriately determined according to the number of wells. For example, a sheet 10C (FIG. 10 (a)) in which 2 holes 12 are arranged vertically and 3 holes in the horizontal direction, and a sheet 10D in which 3 holes 12 are arranged vertically and 4 holes are arranged horizontally (same as above). (B)), 4 sheets vertically and 6 horizontally, a total of 24 sheets 10E (same as (c)), 6 vertically and 8 horizontally, a total of 48 sheets 10F (same (d)) )) Etc. can be used.

In the example shown in each of the above figures, the holes are arranged so as to be arranged in a square lattice, but the holes may be arranged in another arrangement such as a triangular lattice or a honeycomb.

In each of the above examples, paper is used as the material of the sheet, but instead, a plastic plate material such as PET (polyethylene terephthalate), PS (polystyrene), PP (polypropylene), synthetic paper, or the like may be used. .. Further, in each of the above examples, the back surface of the sheet is the adhesive-coated surface or the second described surface, but the adhesive may not be applied to the back surface of the sheet and the information may not be described on the back surface.

10, 10A, 10B, 10C, 10D, 10E, 10F ... Sheet 11 ... Description surface 11B ... Second description surface 12 ... Hole 13 ... Adhesive 81, 83 ... Character 82 ... Symbol 85 ... Bar code 90 ... Microplate 91 ... Top surface of microplate 92 ... Well

Claims (6)

A sheet to be used by being placed on the upper surface of a microplate provided with a plurality of wells, and one surface of the sheet is a writing surface capable of writing with a writing tool or printing with a printing machine. A sheet with holes at positions corresponding to each of the wells. The sheet according to claim 1, wherein the shape of the hole is smaller than the planar shape of the well. The sheet according to claim 1 or 2, wherein an adhesive that adheres to the upper surface of the microplate is applied to the surface opposite to the description surface. The sheet according to claim 1 or 2, wherein the surface opposite to the description surface is a second description surface capable of writing with a writing tool or printing with a printing machine. The sheet according to any one of claims 1 to 4, which has a shape in which a notch is provided in one of four corners of a rectangle or two adjacent corners. The sheet according to any one of claims 1 to 5, wherein a symbol for identifying each of the wells corresponding to each of the holes is described at a position in the vicinity of each of the holes on the description surface. ..

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