JP3201816B2 - External preparation for skin - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin having an effect of inhibiting the production of lipid peroxide, which is useful for preventing skin aging.

[0002]

2. Description of the Related Art There have been many attempts to provide various cosmetically effective ingredients to external preparations, especially skin cosmetics, to provide cosmetics having excellent cosmetic effects. The oxidizing component suppresses the production of lipid peroxide in the skin epidermis, is effective in preventing skin aging, and can be expected to have a cosmetic effect. Has also been done.

[0003] Therefore, various substances having an antioxidant action have been searched, and studies on the effect of preventing skin aging have been carried out. However, the present inventors first found that the fermented solution of lactic acid bacteria had an antioxidant action. I found something. Furthermore, they have found that the lactic acid bacteria fermented liquid has enhanced antioxidant action in combination with vitamin Es, and disclosed a cosmetic using the same (Japanese Patent Publication No. 60-46084).

[0004] On the other hand, various crude drug components considered to be excellent in terms of safety have also been studied, and certain crude drug components have an antioxidant action or an effect similar thereto. (Japanese Patent Application Laid-Open No.
73509, JP-A-60-214721, JP-A-Hei 3
No. 81227).

[0005]

However, the effect of the antioxidant derived from the fermented liquor of lactic acid bacteria or the antioxidant derived from crude drugs is relatively slow, and is not always satisfactory in terms of effect. Therefore, a device for enhancing the effect has been desired.

[0006]

From this viewpoint, the present inventor studied a combination of an antioxidant derived from a fermented lactic acid bacterium solution and an antioxidant derived from a crude drug. And / or combination with extract of liquorice in organic solvent, and further blended with vitamin Es, exerts excellent antioxidant effect. Also, when used as an external preparation for skin, the lipid peroxide of skin It has been found that the formation is suppressed and the coloring of the skin upon irradiation with ultraviolet rays is also suppressed, and the present invention has been completed.

[0007] That is, the present invention provides an external preparation for skin characterized by containing at least one kind of crude drug extract selected from the extract of organic radish and organic extract of licorice, fermented lactic acid bacteria, and vitamin Es. Things.

The lactic acid bacteria fermented liquid used in the present invention is disclosed in Japanese Patent Publication No. 2-24247, one example of a production method of which is described below. And whey is fractionated from the resulting culture. The liquid can be used as it is or, if necessary, by heating it under reduced pressure to evaporate and remove fragrance components.

The lactic acid bacteria used for lactic acid fermentation are lactic acid bacteria commonly used for producing ordinary lactic acid bacteria beverages and fermented milk, such as Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Streptococcus thermophilus. Etc. can be used.

The animal milk used as a culture medium is human milk, milk,
Any milk such as goat milk may be used, and reduced milk from skim milk or powdered milk of these animal milks may be used.

The pentagon extract used in the present invention is an extract of plants of the Labiatae family Scutellaria using hot water or an organic solvent such as ethanol. The extraction method is not particularly limited. For example, it is obtained by extracting with a mixed solvent of water and ethanol, adding cold water to the filtrate of the extract, and collecting the precipitate by filtration. The licorice extract is an extract of a legume licorice plant described in the Japanese Pharmacopoeia with an organic solvent such as ethanol. Similarly, although there is no particular limitation on the extraction method, for example, there is one obtained by extracting an extract with anhydrous ethanol with ethyl acetate and obtaining an oil-soluble fraction. These Orgonum extract and Licorice extract may be used alone or in combination.

The skin preparation for external use of the present invention preferably contains a fermented lactic acid bacterium in an amount of 0.1 to 40% by weight, especially 1 to 20% by weight, in terms of a culture solution. The gogon extract or licorice extract is 0.00005 to 1 in terms of solid matter.
% By weight, particularly preferably 0.0001 to 0.05% by weight.

Further, the skin external preparation of the present invention can further provide an excellent effect of inhibiting the formation of lipid peroxides on the skin and an effect of suppressing coloring upon irradiation with ultraviolet rays by adding vitamin Es to the above components. As such vitamin Es,
dl-α-tocopherol, dl-α-tocopherol fatty acid ester, dl-α-tocopherol nicotinic acid ester and the like. These vitamin Es can be used alone or as a mixture of two or more kinds.
It is preferable to mix by weight%.

In addition to these, various components can be blended as long as the effects of the present invention are not impaired. That is,
Pigments, fragrances, preservatives, stabilizers, fat-soluble vitamins other than vitamin E, oils and fats, humectants, and the like can be blended.

The external preparation for skin of the present invention can be applied not only to various cosmetics including hair cosmetics, but also to any quasi-drugs, pharmaceuticals, toiletry products, etc. as long as they are applied to the skin. It can be made, and the form is not particularly limited, such as cream, liquid, and emulsion.

[0016]

Hereinafter, the present invention will be described in detail with reference to examples.

Production Example 1 Production of lactic acid bacteria fermentation broth; 3 parts by weight of skim milk powder, 97 parts by weight of purified water were added, sterilized by heating, inoculated with a starter of Streptococcus thermophilus, and cultured at 37 ° C. for 24 hours. After completion of the culture, a clear lactic acid bacteria fermented liquid was obtained by filtration.

Production Example 2 Purified water (6 L) was added to 1 kg of Japanese pentagon in Japanese pharmacopoeia.
After heating to 0 ° C., 14 liters of absolute ethanol is added and immersed for 24 hours. Filtration was performed to remove insolubles, and a large excess of purified water was added to the filtrate, and the resulting precipitate was collected by filtration and dried to obtain 100 g of a pentagon extract as a yellow-yellow-green powder.

Production Example 3 10 kg of absolute ethanol is added to 1 kg of licorice root (licorice), and the mixture is extracted under reflux for 5 hours. The obtained ethanol extract was concentrated under reduced pressure, and ethyl acetate was added to the concentrate.
l and extract under reflux for 5 hours. The insolubles were removed by filtration, and the filtrate was concentrated and dried to obtain 30 g of licorice extract as a yellowish brown to reddish brown powder.

Formulation Example 1 (Lotion) The following components were mixed by a conventional method to prepare a lotion. Lactic acid bacteria fermented liquid 25 (% by weight) Acetic acid-dl-α-tocopherol 0.2 Ougon extract (Production example 2) 0.01 Licorice extract (Production example 3) 0.01 Ethanol 5 Glycerin 5 1,3-butylene glycol 5 Methyl paraben 0.05 polyoxyethylene sorbitan monooleate 0.5 carboxyvinyl polymer 0.1 sodium hyaluronate 0.01 fragrance 0.05 purified water balance

Formulation Example 2: (Emulsion) The following components were mixed by a conventional method to produce an emulsion. Lactic acid bacteria fermentation liquid 30 (% by weight) Acetic acid-dl-α-tocopherol 1 Ougone extract (Production Example 2) 0.05 Stearic acid 2 Cetanol 1 Vaseline 5 Liquid paraffin 10 Polyoxyethylene sorbitan monooleate 2 Sorbitan monostearate 2 Butyl paraben 0.05 1,3-butylene glycol 5 carboxymethyl cellulose 0.1 sodium hydroxide 0.05 methyl paraben 0.1 fragrance 0.05 purified water balance

Formulation Example 3 (Cream) The following ingredients were mixed by a conventional method to produce a cream. Fermented solution of lactic acid bacteria 10 (wt%) dl-α-tocopherol 0.001 Licorice extract (Production Example 3) 0.05 Stearic acid 4 Cetanol 2 Vaseline 5 Liquid paraffin 10 Jojoba oil 5 Polyoxyethylene behenyl ether 3 Sorbitan monostearate 3 1,3-butylene glycol 2 butyl paraben 0.05 methyl paraben 0.1 sodium hydroxide 0.05 fragrance 0.05 purified water balance

Test Example 1 on Antioxidant Effect (Inhibition of Lipid Peroxide Production in Human Skin by Irradiation of Ultraviolet Ray and Effect on Skin Color Change) [Test Method] A test was conducted on the lotion prepared in Formulation Example 1 above. The comparison was performed with a control group (coated / non-irradiated, uncoated / irradiated, uncoated / non-irradiated). The skin on the back was tested for two adult males. First, four places on the back are selected for each person, and the space to be tested (one uncoated / non-irradiated point, one coated / non-irradiated point, one uncoated / irradiated point, one applied / irradiated point) ). From the day before the UV irradiation, each sample was applied (twice / day, morning and afternoon), and then UV light was irradiated for 1 hour at about 3500 to 4000 mW by a UV light irradiator (Black light).
After irradiation, the stratum corneum and sebum were sampled by the tape strip method for each irradiated site, and the amount of lipid peroxide was measured and compared. Table 1 shows the results.

[0024]

[Table 1]

The coloration of the skin at each point was also measured before and after the irradiation with a color difference meter, and the change ΔE was compared with the uncoated / irradiated portion as 100%. Table 2 shows the results.

[0026]

[Table 2]

From the above results, it can be seen that the external preparation for skin of the present invention has extremely remarkable effects of increasing the amount of lipid peroxide in the skin and suppressing the coloring of the skin by ultraviolet irradiation.

Test Example 2 for Antioxidant Effect: (Effect of Inhibiting Lipid Peroxide Production in Rat Skin Homogenate) Regarding the effect of each component on inhibiting the production of lipid peroxide,
The test was performed using rat skin homogenate. That is, the skin of male Wister rats weighing about 290 g was collected, added with cold physiological saline, and treated with Polytron to obtain a 2% skin homogenate. A reaction system was prepared by adding 8 ml of skin homogenate, 1 ml of purified water and 1 ml of ethanol in a Petri dish, and each test sample (lactic acid bacteria fermented solution 10%, tocopherol acetate 0.1%, gongon extract 0.001%, licorice) 0.0002% of the extract, a mixture of the four, and a control group without the addition (6 points) were added. After leaving at room temperature for 30 minutes, ultraviolet rays (UV-A) were irradiated for 30 minutes. Next, the amount of lipid peroxide produced in each sample was determined by the OBA et al.
3, p660, 1978). The results are shown in Table 3 (represented by the percentage of suppression based on the amount of production in the non-added group).

[0029]

[Table 3]

[0030]

Industrial Applicability According to the present invention, it has become possible to provide a skin external preparation having an excellent antioxidant action and lipid peroxide-suppressing action. Further, the external preparation for skin of the present invention is particularly useful as a cosmetic having an excellent antiaging effect on skin and an excellent cosmetic effect.

Continued on the front page (72) Inventor Takako Koyama 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Yakult Honsha Co., Ltd. (72) Inventor Minoru Ichioka 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Yakult Co., Ltd. In-house (56) References JP-A-3-81227 (JP, A) JP-A-59-73509 (JP, A) JP-A-2-111710 (JP, A) JP-A-2-36113 (JP, A) JP-A-63-23809 (JP, A) JP-A-60-214721 (JP, A) JP-A-1-256587 (JP, A) JP-A-59-4432 (JP, A) JP-A-5-205 70334 (JP, A) JP 60-46084 (JP, B2) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (1)

(57) [Claims] Claims: 1. A herbal extract selected from the organic solvent extract of Orgonum extract and the extract of Licorice,
An external preparation for skin, comprising a fermented solution of lactic acid bacteria and vitamin Es.