JP3190347B2 - 電気泳動分取装置 - Google Patents
電気泳動分取装置Info
- Publication number
- JP3190347B2 JP3190347B2 JP51496295A JP51496295A JP3190347B2 JP 3190347 B2 JP3190347 B2 JP 3190347B2 JP 51496295 A JP51496295 A JP 51496295A JP 51496295 A JP51496295 A JP 51496295A JP 3190347 B2 JP3190347 B2 JP 3190347B2
- Authority
- JP
- Japan
- Prior art keywords
- electrophoresis
- cells
- members
- electrophoretic separation
- passage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000926 separation method Methods 0.000 title claims description 41
- 238000001962 electrophoresis Methods 0.000 claims description 67
- 238000005194 fractionation Methods 0.000 claims description 21
- 239000000126 substance Substances 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 5
- 239000000243 solution Substances 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 102000036675 Myoglobin Human genes 0.000 description 8
- 108010062374 Myoglobin Proteins 0.000 description 8
- 102000009027 Albumins Human genes 0.000 description 5
- 108010088751 Albumins Proteins 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 125000006850 spacer group Chemical group 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 4
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 4
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 4
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 4
- 238000001155 isoelectric focusing Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000013076 target substance Substances 0.000 description 4
- 238000005251 capillar electrophoresis Methods 0.000 description 3
- 102000004338 Transferrin Human genes 0.000 description 2
- 108090000901 Transferrin Proteins 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 description 2
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 description 2
- 229940024142 alpha 1-antitrypsin Drugs 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 239000012581 transferrin Substances 0.000 description 2
- 229920000178 Acrylic resin Polymers 0.000 description 1
- 239000004925 Acrylic resin Substances 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Electrostatic Separation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5-298583 | 1993-11-29 | ||
| JP29858393 | 1993-11-29 | ||
| PCT/JP1994/001999 WO1995014923A1 (fr) | 1993-11-29 | 1994-11-29 | Fractionneur d'electrophorese |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP3190347B2 true JP3190347B2 (ja) | 2001-07-23 |
Family
ID=17861624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP51496295A Expired - Fee Related JP3190347B2 (ja) | 1993-11-29 | 1994-11-29 | 電気泳動分取装置 |
Country Status (4)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017175817A1 (ja) | 2016-04-07 | 2017-10-12 | 株式会社ホットアルバム炭酸泉タブレット | 炭酸ガスマイクロバブル混合水の製造方法及び製造器 |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2016353A1 (en) * | 1989-05-23 | 1990-11-23 | Charles E. Winslow, Jr. | Method for re-use of aqueous co-product from manufacture of sodium dithionite |
| US5540826A (en) * | 1995-03-15 | 1996-07-30 | Protein Technologies, Inc. | Multi-channel separation device |
| AUPP108697A0 (en) * | 1997-12-23 | 1998-01-22 | Macquarie Research Limited | Apparatus for electrophoresis |
| AU736903B2 (en) * | 1997-12-23 | 2001-08-02 | Macquarie Research Limited | Apparatus for electrophoresis |
| US10337054B2 (en) | 2004-02-02 | 2019-07-02 | Quantum-Si Incorporated | Enrichment of nucleic acid targets |
| US8529744B2 (en) | 2004-02-02 | 2013-09-10 | Boreal Genomics Corp. | Enrichment of nucleic acid targets |
| US8133371B2 (en) * | 2004-02-02 | 2012-03-13 | The University Of British Columbia | Scodaphoresis and methods and apparatus for moving and concentrating particles |
| CA2496294A1 (en) | 2005-02-07 | 2006-08-07 | The University Of British Columbia | Apparatus and methods for concentrating and separating particles such as molecules |
| US8475641B2 (en) * | 2008-02-01 | 2013-07-02 | The University Of British Columbia | Methods and apparatus for particle introduction and recovery |
| US8877028B2 (en) | 2009-04-21 | 2014-11-04 | The University Of British Columbia | System and methods for detection of particles |
| CA2742327A1 (en) | 2011-05-20 | 2012-11-20 | The University Of British Columiba | Systems and methods for enhanced scoda |
| WO2013104994A2 (en) | 2012-01-13 | 2013-07-18 | The University Of British Columbia | Multiple arm apparatus and methods for separation of particles |
| EP2844773B1 (en) | 2012-05-04 | 2017-08-16 | Boreal Genomics Corp. | Biomarker analysis using scodaphoresis |
| US9340835B2 (en) | 2013-03-15 | 2016-05-17 | Boreal Genomics Corp. | Method for separating homoduplexed and heteroduplexed nucleic acids |
| US11130986B2 (en) | 2015-05-20 | 2021-09-28 | Quantum-Si Incorporated | Method for isolating target nucleic acid using heteroduplex binding proteins |
| BR112022008098A2 (pt) | 2019-10-29 | 2022-07-12 | Quantum Si Inc | Bombeamento peristáltico de fluidos e métodos, sistemas e dispositivos associados |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3240692A (en) * | 1961-03-13 | 1966-03-15 | Swift & Co | Electrophoretic fractionation of ampholytes |
| JPS5064180A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1973-10-09 | 1975-05-31 | ||
| US4911807A (en) * | 1989-09-05 | 1990-03-27 | Bio-Rad Laboratories, Inc. | Fractionation and sample loading by cassette in capillary electrophoresis |
| JP2850529B2 (ja) * | 1990-11-22 | 1999-01-27 | 横河電機株式会社 | 液体試料分析装置 |
| DE59108591D1 (de) * | 1991-12-06 | 1997-04-10 | Ciba Geigy Ag | Elektrophoretische Trennvorrichtung und elektrophoretisches Trennverfahren |
-
1994
- 1994-11-29 WO PCT/JP1994/001999 patent/WO1995014923A1/ja not_active Application Discontinuation
- 1994-11-29 EP EP95901605A patent/EP0682248A1/en not_active Withdrawn
- 1994-11-29 JP JP51496295A patent/JP3190347B2/ja not_active Expired - Fee Related
- 1994-11-29 US US08/495,674 patent/US5609743A/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017175817A1 (ja) | 2016-04-07 | 2017-10-12 | 株式会社ホットアルバム炭酸泉タブレット | 炭酸ガスマイクロバブル混合水の製造方法及び製造器 |
Also Published As
| Publication number | Publication date |
|---|---|
| US5609743A (en) | 1997-03-11 |
| EP0682248A4 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1995-12-20 |
| EP0682248A1 (en) | 1995-11-15 |
| WO1995014923A1 (fr) | 1995-06-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |