JP3182243B2 - 一本鎖ｄｎａ−ｒｎａハイブリッド分子の合成方法 - Google Patents

一本鎖ｄｎａ−ｒｎａハイブリッド分子の合成方法

Info

Publication number
JP3182243B2
JP3182243B2 JP01677793A JP1677793A JP3182243B2 JP 3182243 B2 JP3182243 B2 JP 3182243B2 JP 01677793 A JP01677793 A JP 01677793A JP 1677793 A JP1677793 A JP 1677793A JP 3182243 B2 JP3182243 B2 JP 3182243B2
Authority
JP
Japan
Prior art keywords
msdna
rna
dna
reverse transcriptase
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP01677793A
Other languages
English (en)
Japanese (ja)
Other versions
JPH06319565A (ja
Inventor
スミコ イノウエ
マサヨリ イノウエ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers Health
Original Assignee
University of Medicine and Dentistry of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Medicine and Dentistry of New Jersey filed Critical University of Medicine and Dentistry of New Jersey
Publication of JPH06319565A publication Critical patent/JPH06319565A/ja
Application granted granted Critical
Publication of JP3182243B2 publication Critical patent/JP3182243B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP01677793A 1992-01-06 1993-01-06 一本鎖ｄｎａ−ｒｎａハイブリッド分子の合成方法 Expired - Fee Related JP3182243B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/817430 1992-01-06
US07/817,430 US5434070A (en) 1989-02-24 1992-01-06 Reverse transcriptases from Escherichia coli and Myxococcus xanthus

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2001055213A Division JP2001258582A (ja) 1992-01-06 2001-02-28 一本鎖ｄｎａ−ｒｎａハイブリッド分子

Publications (2)

Publication Number Publication Date
JPH06319565A JPH06319565A (ja) 1994-11-22
JP3182243B2 true JP3182243B2 (ja) 2001-07-03

Family

ID=25223072

Family Applications (2)

Application Number Title Priority Date Filing Date
JP01677793A Expired - Fee Related JP3182243B2 (ja) 1992-01-06 1993-01-06 一本鎖ｄｎａ−ｒｎａハイブリッド分子の合成方法
JP2001055213A Pending JP2001258582A (ja) 1992-01-06 2001-02-28 一本鎖ｄｎａ−ｒｎａハイブリッド分子

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2001055213A Pending JP2001258582A (ja) 1992-01-06 2001-02-28 一本鎖ｄｎａ−ｒｎａハイブリッド分子

Country Status (4)

Country Link
US (1) US5434070A (enExample)
EP (1) EP0562206B1 (enExample)
JP (2) JP3182243B2 (enExample)
DE (1) DE69232768T2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713482B2 (en) 2003-12-18 2010-05-11 The Clorox Company Control scheme for enhanced filtered water systems

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2675803B1 (fr) * 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
DE69604051T2 (de) * 1995-01-27 1999-12-16 The Government Of The United States Of America, As Represented By The Secretary Verfahren zum sensitiven nachweis von reverser transcriptase
EP0862651A2 (en) * 1995-10-16 1998-09-09 Chiron Corporation Method of screening for factors that modulate gene expression
PL343064A1 (en) * 1998-03-20 2001-07-30 Benitec Australia Ltd Control of gene expression
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CA2345936A1 (en) * 1998-10-09 2000-04-20 Ingene, Inc. Production of ssdna in a cell
AU2004205192B2 (en) * 1998-10-09 2007-09-20 Cytogenix, Inc. Production of ssDNA in vivo
KR20010099682A (ko) * 1998-10-09 2001-11-09 추후보충 단일가닥 ｄｎａ의 효소 합성
US6423885B1 (en) * 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US7419964B2 (en) 1999-09-16 2008-09-02 Cytogenix, Inc. Treatment of HSV-related pathologies using ssDNA
WO2003093424A2 (en) * 2002-05-01 2003-11-13 Cytogenix, Inc. In vivo ssdna expression vectors for altering gene expression
SE0202867D0 (sv) * 2002-09-27 2002-09-27 Pyrosequencing Ab New sequencing method
MX2022003091A (es) * 2019-09-12 2022-06-08 The J David Gladstone Inst A Testamentary Trust Established Under The Will Of J David Gladstone Retroelemento bacteriano modificado con produccion de adn mejorada.
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Ａｎｎ．Ｒｅｖ．Ｍｉｃｒｏｂｉｏｌ．，Ｖｏｌ．４５，Ｐ．１６３−１８６（１９９１）
Ｃｅｌｌ，Ｖｏｌ．５６，Ｎｏ．４，Ｐ．７０１−７０７（１９８９）
Ｊ．Ｂａｃｔｅｒｉｏｌ．，Ｖｏｌ．１７３，Ｎｏ．１３，Ｐ．４１７１−４１８１（１９９１）
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，Ｖｏｌ．２６５，Ｎｏ．１５，Ｐ．８４９０−８４９６（１９９０）
Ｐｒｏｇｒｅｓｓ ｉｎ Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ．，Ｖｏｌ．４０，Ｐ．１−２４（１９９１）
Ｓｃｉｅｎｃｅ，Ｖｏｌ．２４３，Ｐ．１０３３−１０３８（１９８９）

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713482B2 (en) 2003-12-18 2010-05-11 The Clorox Company Control scheme for enhanced filtered water systems

Also Published As

Publication number Publication date
JPH06319565A (ja) 1994-11-22
US5434070A (en) 1995-07-18
JP2001258582A (ja) 2001-09-25
DE69232768D1 (de) 2002-10-17
DE69232768T2 (de) 2003-06-12
EP0562206A3 (enExample) 1995-06-28
EP0562206B1 (en) 2002-09-11
EP0562206A2 (en) 1993-09-29

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