JP3122167B2 - Artemisia extract and use thereof - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mugwort extract having good stability and little coloring on clothes, and containing it to heal wounds, prevent rough skin and improve rough skin, as well as itchy skin, body odor and bad breath. The present invention relates to cosmetics, detergent compositions, oral compositions, and foods having excellent effects such as prevention of bleeding.

[0002]

2. Description of the Prior Art Conventionally, extracts of water or water-containing alcohol of mugwort are blended with various compositions for the purpose of healing wounds, preventing rough skin, improving rough skin, and preventing skin itching, body odor and bad breath. It has been. However, the extract of water or hydroalcoholic water of mugwort originally has a dark brown to brown color.For example, when used directly in cosmetics, this color adheres to clothes or when cosmetics containing this are stored for a long time. There were problems in stability such as browning and precipitation. Until now, activated carbon treatment has been used to solve this problem, but the drawback of losing the effects of wound healing, preventing rough skin, improving rough skin, preventing skin itching, body odor and bad breath. was there. Further, an extract obtained from the beginning using an organic solvent such as hexane, ethyl acetate, acetone, ethanol, and methanol contains terpene and dark chlorophyll, which are problematic in safety, and has a problem in use. These effects were not yet satisfactory.

[0003]

In view of these circumstances, the present inventors have conducted intensive studies and found that the average molecular weight is 1
5000 proteins were found to be the cause of browning and precipitation of mugwort extract, and mugwort extract which substantially removed this was significantly more stable and less colored on clothes than the original extract, The present inventors have found that they have excellent effects such as wound healing, prevention of rough skin, improvement of rough skin, and prevention of itching, body odor and bad breath of the skin, and have completed the present invention.

[0004]

[Means for Solving the Problems] That is, the present invention provides:
A mugwort extract from which proteins having an average molecular weight of 15,000 have been substantially removed. And a cosmetic, a detergent composition, an oral composition, and a food, comprising the mugwort extract. Hereinafter, the present invention will be described in detail.

[0005] The mugwort extract of the present invention can be obtained, for example, by the following method. The mugwort is heated to reflux or soaked with one or two or more of water-containing alcohols such as water or water-containing methanol, water-containing ethanol, water-containing butanol, water-containing propanol, water-containing 1,3-butylene glycol, and water-containing glycerin, filtered, and filtered. A crude extract is obtained. At this time, in order to remove a hydrophobic component, it may be previously extracted with a non-polar solvent such as hexane.

[0005] Next, the water or water-containing ethanol extract of mugwort obtained in this way is treated by, for example, gel permeation chromatography set under the following conditions, and the initial water effluent of the retention time of 0 to 25 minutes is substantially reduced. By removing it, a mugwort extract having good target stability and little coloring on clothes can be obtained.

Column: Rover column 3 cm inner diameter × 30 cm length Filler: Cross-linked dextran oxypropyl ether gel (for example, Sephadex LH-20 manufactured by Pharmacia Co., Ltd. Column temperature: room temperature Solvent: 200 ml of water → 70 % Acetone 100 ml Flow rate: 2.0 ml / min Sample amount: Artemisia hot water extract 5 ml Detection: wavelength 254 nm

Further, it can be obtained by treating the gel permeation chromatography under the following conditions to substantially remove the initial water effluent at a retention time of 0 to 50 minutes.

[0008] Column: packed column for high-performance liquid chromatography, inner diameter 7.5 mm x length 30 cm Filler: hydrophilic silica gel (for example, Tosoh Corporation)
Solvent: 0.2 M phosphate buffer Flow rate: 0.5 ml / min Sample volume: 100 μl Detection: wavelength 280 nm

FIG. 1 shows a chromatogram in which a protein having an average molecular weight of 15,000 contained in a water or hydroalcoholic extract of mugwort was detected by gel permeation chromatography set under the above conditions.

[0010] Further, even if a mugwort water or hot water extract is treated as follows, a low sensitizing mugwort extract of the same quality can be obtained. That is, water or hot water extract of mugwort is re-extracted with 50% to 100% of one or more kinds of water-containing alcohols such as water-containing ethanol, water-containing methanol, water-containing butanol, water-containing 1,3-butylene glycol, and water-containing glycerin. It is obtained by filtering and removing the insoluble portion. Alternatively, conversely, it can be obtained by first extracting with a polar organic solvent such as ethyl acetate, acetone, ethanol, or methanol and then re-extracting this extract with water or hot water, and filtering and removing the insoluble portion. Can be
Also at this time, in order to remove the hydrophobic component, it may be previously extracted with a non-polar solvent such as hexane.

[0011] The compounding amount of the mugwort extract in the cosmetic according to the present invention is 0.00001 to 5 as a dry matter in the total amount of the cosmetic.
%, Preferably 0.0005 to 1%. If the amount is less than 0.00001%, the effects of the present invention cannot be sufficiently exerted, which is not preferable.

[0012] The amount of the mugwort extract in the cleaning composition according to the present invention is as dry matter in the total amount of the cleaning composition.
0.00001 to 5%, preferably 0.0005 to 1%. 0.0000
If it is less than 1%, the effects of the present invention cannot be sufficiently exerted, which is not preferable.

[0013] The amount of the mugwort extract in the oral composition according to the present invention is as dry matter in the total amount of the oral composition.
0.00001 to 5%, preferably 0.0005 to 1%. 0.0000
If it is less than 1%, the effects of the present invention cannot be sufficiently exerted, which is not preferable.

The content of the mugwort extract in the food according to the present invention is 0.00001 to 5%, preferably 0.0005 to 1% as a dry matter in the whole food. If it is less than 0.00001%,
The effect of the present invention is not sufficiently exhibited, which is not preferable.

The cosmetics, detergent compositions, oral composition cosmetics, and foods of the present invention may be added to each of the above-mentioned essential components, if necessary, as long as the effects of the present invention are not impaired. Various components generally used can be blended.

For example, powder components such as titanium dioxide, mica, and talc, and pigments. Avocado oil, macadamia nut oil, corn oil, olive oil, rapeseed oil, evening primrose oil, castor oil, sunflower oil, teaseed oil, rice bran oil, jojoba oil, cocoa butter, coconut oil, squalene, squalane, beef tallow, mokurou, beeswax, can Delila wax, carnauba wax, whale wax, lanolin, liquid paraffin, ceresin,
Oils such as petrolatum, polyoxyethylene (8 mol) oleyl alcohol ether and glyceryl monooleate. Caprylic alcohol, lauryl alcohol, myristyl alcohol, cetyl alcohol, cholesterol,
Higher alcohols such as phytosterols, capric acid,
Higher fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, lanolin fatty acid, linoleic acid and linolenic acid. Para-aminobenzoic acid, homomenthyl-7N-acetylanthraniate, butylmethoxybenzoylmethane, di-paramethoxycinnamic acid-mono-
UV absorbers such as glyceryl 2-ethylhexanoate, amyl salicylate, octylcinnamate, and 2,4-dihydroxybenzophenone.

Polyethylene glycol, glycerin, sorbitol, xylitol, maltitol, mucopolysaccharide,
Moisturizers such as hyaluronic acid, chondroitin sulfate, chitosan, carboxymethyl chitin (salt). Methylcellulose, ethylcellulose, carboxymethylcellulose,
Thickeners such as gum arabic, polyvinyl alcohol, montmorillonite and saponite. Organic solvents such as ethanol and 1,3-butylene glycol. Antioxidants such as butylhydroxytoluene, tocopherol and phytic acid. Antibacterial preservatives such as benzoic acid, salicylic acid, sorbitanic acid, dehydroacetic acid, alkyl paraoxybenzoate (ethyl paraben, butyl paraben, etc.) hexachlorophene. Glycine, alanine, valine, leucine,
Amino acids such as serine, threonine, phenylalanine, tyrosine, aspartic acid, glutamic acid, asparagine, glutamine, taurine, arginine and histidine, and alkali metal salts and hydrochlorides thereof. Acyl sarcosine salts (for example, sodium lauroyl cocin), glutathione, citric acid, malic acid, tartaric acid, organic acids such as lactic acid, vitamin A and its derivatives, vitamin B6 hydrochloride,
Vitamin B6 tripalmitate, vitamin B6 dioctanoate, vitamin B2 and its derivatives, vitamin B1
2, vitamin B such as vitamin B15 and its derivatives,
Vitamin Cs such as ascorbic acid, ascorbic acid sulfate, ascorbic acid phosphate, ascorbic acid dipalmitate, α-tocopherol, β-tocopherol, γ-tocopherol, vitamin Es such as vitamin E acetate, vitamin E nicotinate, vitamin D And vitamins such as vitamin H, pantothenic acid, and pantethine. Nicotinamide, benzyl nicotinate, γ-oryzanol, allantoin, glycyrrhizic acid (salt), glycyrrhetinic acid and its derivatives, hinokitiol, mucidin, bisabolol, eucalyptol,
Various drugs such as phytosterol, thymol, inositol, saponins (such as saikosaponin, carrot saponin, hemimasaponin, muclodisaponin), pantothenyl ethyl ether, ethinyl estradiol, cepharanthin, and placenta extract.

Licorice, Paprika, Oguruma, Beninoki, Rumex, Clara, Camphor tree, Kouhone, Dokudami, Haikazura, Celery, Geranium, Turmeric, Odorikosou, Orange, Sage, Sycamore, Nagiida, Mistletoe, Zeniaoi, Senkyu, Senburi Clove, cock, sprout, spruce, carrot,
Garlic, wild rose, birch, parsley, gentian, peppermint, fennel, horsetail, saffron, Dutch mustard,
Sapon saw, butcher bloom, grapes, ivy,
Loofah, nettle, bodaige, hop, salamander,
Shiitake mushrooms, horse chestnuts, honeysuckle, muk roe, melissa, peaches, eucalyptus, jiou, sicon, yukinoshita, lily, perilla, peonies, rosemary, lemon, ginger, eijitsu, waremoko, birch, raspberry,
Ogun, Aloe, Cucumba, Burdock, Gardenia,
A natural extract obtained by extracting oak, spinach, ashenyak, amacha, diatom, shinobuhiba, sawara, capsicum, bakuryo, sarnokoshake, choreimaitake, mannentake, and red algae with an organic solvent, alcohol, polyhydric alcohol, water, hydrous alcohol, and the like.

Sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monostearate, polyoxyethylene glycol monooleate, poly Nonionic surfactants such as oxyethylene alkyl ether, polyglycol diester, lauryl diethanol amide, fatty acid diethanol amide, cationic surfactants such as stearyl trimethyl ammonium chloride, benzalkonium chloride, sodium palmitate, sodium laurate, lauryl Sodium sulfate, potassium lauryl sulfate, alkyl ethanol triethanolamine ether,
Anionic and amphoteric surfactants such as funnel oil, linear dodecylbenzene sulfate, polyoxyethylene hydrogenated castor oil and maleic acid. Perfume, purified water, etc. Also,
The cosmetics of the present invention, the detergent composition, the oral composition, and the dosage form of the food are arbitrary, for example, a solubilizing system such as a lotion,
Emulsified systems such as emulsions and creams, or dosage forms such as dispersions and ointments can be used.

[0020]

Next, the effects of the present invention will be described in detail with reference to examples, but this does not limit the scope of the present invention.

Example 1 An extract obtained by adding 500 ml of water to 100 g of mugwort and heating in a water bath for 2 hours was treated by gel permeation chromatography set under the following conditions. The chromatogram obtained at this time is shown in FIG. By removing the initial water effluent from the retention time of 0 to 25 minutes in FIG.
8.0 g of the desired hyposensitizing mugwort extract was obtained. Column: Rover column Inner diameter 3 cm × Length 30 cm Filler: Crosslinked dextranoxypropyl ether gel (for example, Sephadex LH-20 manufactured by Pharmacia) Column temperature: room temperature Solvent: 200 ml of water → 70% acetone Flow rate: 100 ml Flow rate: 2.0 ml / min Sample amount: 5 ml of hot water extract of mugwort Detection: wavelength of 254 nm FIG. 2 shows a chromatogram of a heated extract, and a peak of a protein having an average molecular weight of 15,000 is seen at a retention time of 25 minutes. .

Example 2 1000 ml of water was added to 100 g of mugwort and heated in a water bath for 2 hours to concentrate the hot water extract.
The mixture was re-extracted with 00 ml, and the insoluble portion was filtered and concentrated to obtain 3.3 g of an extract. This was subjected to gel permeation chromatography set under the following conditions, and it was confirmed that the protein having an average molecular weight of 15,000 detected in FIG. 1 was removed. In this case, FIG. 3 shows a chromatogram of the obtained mugwort extract of the present invention, and FIG. 4 shows a chromatogram of the crude extract which has not been re-extracted with 70% ethanol. In FIG. 4, the retention time 4
At 0 minutes, a peak derived from a protein having an average molecular weight of 15000 is observed, but in FIG. 5, a peak rises at a retention time of 50 minutes, indicating that the protein has been removed. Column: packed column for high-performance liquid chromatography Packing agent: hydrophilic silica gel (for example, Tosoh Corporation)
Solvent: 0.2 M phosphate buffer Flow rate: 0.5 ml / min Sample amount: Artemisia hot water extract 100 μl Detection: wavelength 280 nm

Example 3 400 ml of ethanol was added to an extract obtained by adding 800 ml of water to 100 g of mugwort and immersing and extracting for 3 days, and the insoluble portion was filtered and concentrated to obtain 5.4 g of extract. It was confirmed as follows that the substance having a molecular weight of 15,000 substantially removed in the above Examples was a protein. FIG.
1 shows the infrared absorption spectrum of the compound having a molecular weight of 15,000 shown in FIG. 1 (lower row) and the infrared absorption spectrum of the enzyme protein (protease) (upper row). The major absorptions of the two are fairly consistent, the molecular weight of this compound is as large as 15,000, and the compound reacts with a protein color reagent (eg, a protein assay kit manufactured by Bio-Rad Inc.). This compound was determined to be a protein.

Next, in order to compare the stability and color strength of the mugwort extract thus treated with the untreated product, each extract was adjusted to a concentration of 1%, and the extract was adjusted to 1% at 50 ° C. It was stored for months and examined for the presence of a precipitate. In addition, a wavelength of 440 nm
Was measured for absorbance. As untreated products, a hot water extract of mugwort, a water immersion extract, a 70% aqueous 1,3-butylene glycol extract, and a 70% aqueous ethanol extract were used.
The test was performed. Table 1 shows the presence or absence of precipitation after storage for one month.
Table 2 shows the absorbance at a wavelength of 440 nm.

[0025]

[Table 1] 有無 Presence or absence of sample Sample Before test After test ────────── ───────────────── Example 1 None None Example 2 None None Example 3 None None Hot water extract None Gray precipitate Water immersion extract None Gray precipitate 70% water content 1 , 3-BG extract None Brown precipitate 70% aqueous ethanol extract None Brown precipitate ───────────────────────────

[0026]

[Table 2] 有無 Presence or absence of sample Sample Before test After test ────────── ───────────────── Example 1 0.53 0.67 Example 2 0.72 0.83 Example 3 0.61 0.76 Hot water extract 2.05 3.72 Water immersion extract 1.78 3.87 70% water content 1,3 -BG extract 1.53 2.53 70% aqueous ethanol extract 2.61 4.91 ───────────────────────────

As shown in Tables 1 and 2, the mugwort extract obtained by the present invention has improved stability and lighter color compared to the conventional mugwort extract, despite the same concentration, and thus, There are few problems such as color sticking to clothes. In addition, in order to show effects such as wound healing, prevention of rough skin, improvement of rough skin, and prevention of skin itch and body odor, tests of cell growth promoting action and deodorizing effect of ethyl mercaptan were performed. Next, in order to show the effect of preventing skin itch, senile pruritus, etc., an effect test of bradykinin-induced pruritus and a practical use test were performed.

(Cell proliferation promoting action) Human skin tissue was sliced and attached to the bottom of a cell culture dish, and Eagle's
When cultured in an sMEM culture solution (containing 10% fetal bovine serum) for one week, the bottom surface of the Petri dish is almost completely filled with fibroblasts. The fibroblasts were treated with a 0.25% trypsin solution to make single cells, and then 10,000 cells / m
l of cell suspension, and add this solution
In addition, Eagle's MEM culture solution and various mugwort extracts (final concentration 1 μl / ml) were further added and cultured in a CO2 incubator for 2 weeks, and then the cells were fixed and stained.
Cell colonies were counted. The case where no mugwort extract was added was used as a control. In addition, the cell growth promotion rate is a value obtained by dividing the number of colonies of cells treated with various mugwort extracts by the number of colonies of control cells and multiplying by 100.

Table 3 shows the cell growth promotion rate after two weeks of culture. The determination method is as follows. -Judgment-：: Cell growth promotion rate of 150% or more ：: Cell growth promotion rate of 120% to less than 150% △: Cell growth promotion rate of 100% to less than 120% ×: Cell growth promotion rate of less than 100%

[0030]

[Table 3]

The extracts obtained in Examples 1 to 3 showed a stronger cell growth promoting effect than any of the untreated extracts. (Deodorizing effect on ethyl mercaptan) Various mugwort extracts were put into a 50 ml screw tube, and 10 m of water was added thereto.
and 10 μl of ethyl mercaptan were added, and 10 minutes later, the amount of ethyl mercaptan present in the upper space of the screw tube was quantified by head space gas chromatography. Table 4 shows the residual ratio of ethyl mercaptan with respect to the blank. The determination method is as follows. -Judgment-：: Residual rate of less than 10% ：: Residual rate of 10% to less than 50% △: Residual rate of 50% to less than 80% ×: Residual rate of 80% or more

[0032]

[Table 4]

As can be seen from Table 4, the extracts obtained in Examples 1 to 3 had a higher deodorizing effect than any of the untreated extracts. (Test for the effect of preventing bradykinin-induced pruritus) A 5% solution of bradykinin on the back skin of Hartley-type albino guinea pig
Treated with 0.5 ml. Thereafter, a lotion having a basic formulation shown in Table 5 was produced, and a mixture containing 5% by weight of each sample was applied thereto. The behavior of 10 guinea pigs per group was observed for 3 hours, and the total score was obtained by multiplying the number of each behavior by the score according to the following criteria.

[0034]

[Table 5] Lotion basic formula 成分 Ingredient blending amount (% by weight) ─────────── ────────────── glycerin 2.0 propylene glycol 1.0 citric acid 0.2 95% ethanol 10.0 perfume qs POE (20) lauryl ether 0.5 purified water residue ─────────── ──────────────

-Judgment criteria-Rating Evaluation 1 The injection site is licked. 2. Scratching the injection site with hind legs. Next, the prevention rate (%) of bradykinin-induced pruritus was determined by the following equation (1).
Asked by.

[0036]

(Equation 1)

Table 6 shows the effect of each sample on the prevention of bradykinin-induced pruritus. The determination method is as follows. -Judgment of prevention effect of bradykinin-induced pruritus-A: Prevention rate of pruritus (%) 75% or more ○: Prevention rate of pruritus (%) 50% or more and less than 75% △: Prevention rate of pruritus (%) 25% or more 50 %: Less than 25% pruritus prevention rate (%)

[0038]

[Table 6] Preventive effect of bradykinin-induced pruritus ─────────────────────────────── Sample Preventive effect of pruritus ──── ─────────────────────────── Lotion only × Example 1 combination lotion ◎ Example 2 combination lotion ◎ Example 3 combination lotion ◎ mugwort water Lotion with soaked extract △ Lotion with mugwort hot water extract ○ Lotion with mugwort 70% water-containing 1,3-BG extract △ lotion with mugwort 70% aqueous ethanol extract ──────────────────────────────

As described above, the lotion containing Examples 1, 2, and 3 showed a strong effect of preventing bradykinin-induced pruritus.

(Actual use test) The effect of preventing itching of the skin by the actual use test is shown below. -Test method- For 50 elderly people in nursing homes suffering from senile pruritus, a double-blind lotion containing 2 preparations, a lotion containing mugwort hot water extract and a basic prescription lotion of placebo were given for 1 day. Two applications were applied, and two weeks later, the effects were compared by questioning. The evaluation is shown below. Rating Evaluation 1 Itching worsened. 2 Same as before. 3 Itching was slightly improved. 4 Itching was improved. 5 I no longer feel itchy. Judgment was made on the basis of the rating according to the following criteria. ：: The percentage of panels evaluated as 4 and 5 is 75% or more Δ: The percentage of panels evaluated as 4 and 5 is 25% or more and less than 75% ×: The percentage of panels evaluated as 4 and 5 Is less than 25%. Table 7 shows the results.

[0041]

[Table 8]

As is clear from Tables 6 and 7, the external preparation for skin of the present invention is a novel external preparation for skin having an excellent anti-itch effect on the skin. Next, each composition containing the mugwort extract of the present invention will be described in detail according to formulation examples. In addition, this invention is not limited by this. The compounding amount is% by weight, and the compounding amount of various hyposensitizing mugwort extracts is described as a dried product.

Example 4 Lotion (1) Artemisia extract obtained in Example 1 1.0 (2) Glycerin 4.0 (3) 1,3-butylene glycol 4.0 (4) Ethanol 7.0 ( 5) polyoxyethylene oleyl alcohol 0.5 (6) methyl paraben 0.05 (7) citric acid 0.01 (8) sodium citrate 0.1 (9) fragrance 0.05 (10) purified water residue (production method) Citric acid, sodium citrate, glycerin, 1,3-butylene glycol, and the mugwort extract obtained in Example 1 are dissolved in purified water. Separately, polyoxyethylene oleyl alcohol, a fragrance, and methyl paraben were dissolved in ethanol, added to the above-mentioned purified aqueous solution to solubilize it, and filtered to obtain a lotion.

Example 5 Cream (1) Cetostearyl alcohol 3.5 (2) Squalane 40.0 (3) Beeswax 3.0 (4) Reduced lanolin 5.0 (5) Ethyl paraben 0.3 (6) Poly Oxyethylene (20) sorbitan monopalmitic acid 2.0 ester (7) Stearic acid monoglyceride 2.0 (8) Artemisia extract obtained in Example 0.005 (9) Fragrance 0.03 (10) 1, 3-butylene glycol 5.0 (11) Glycerin 5.0 (12) Sodium hyaluronate 0.05 (13) Purified water residue (Production method) (1) to (9) were heated and dissolved, and kept at 75 ° C. The mixture was added to (10) to (13) heated to 75 ° C with stirring. After processing with a homomixer to reduce the emulsified particles,
The mixture was quenched with stirring to obtain a cream.

Example 6 Emulsion (1) Artemisia extract 0.5 obtained in Example 3 (2) Stearic acid 1.5 (3) Cetyl alcohol 0.5 (4) Beeswax 2.0 (5) Polyoxy Ethylene (10) 1.0 1.0 Nooleic acid ester (6) Glycerin monostearic acid 1.0 ester (7) Quince seed extract 20.0 (5% aqueous solution) (8) Propylene glycol 5.0 (9) Ethanol 3 0.0 (10) Ethylparaben 0.3 (11) Fragrance 0.03 (12) Purified water residue (Preparation method) The mugwort extract obtained in Example 3 and a flavor are added to ethanol and dissolved. (Alcohol phase) Propylene glycol is added to purified water, dissolved by heating, and maintained at 70 ° C.
(Aqueous phase) Other components except the quince seed extract are mixed, heated and dissolved, and kept at 70 ° C. (Oil phase) The oil phase is added to the aqueous phase, pre-emulsification is performed, and the mixture is uniformly emulsified with a homomixer. This was cooled to 30 ° C. while stirring to obtain an emulsion.

Example 7 Pack (1) Artemisia extract obtained in Example 1 0.1 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) Ethanol 10.0 (6) Methyl paraben 0.05 (7) Fragrance 0.05 (8) Purified water residue (Preparation method) To the purified water, add the mugwort extract obtained in Example 1, polyethylene glycol, propylene glycol, and methyl paraben and stir. Dissolve. Next, polyvinyl alcohol was added and the mixture was stirred with heating, and ethanol in which a fragrance was dissolved was added and dissolved by stirring to obtain a pack.

Example 8 Cosmetic for scalp (scalp treatment) (1) Artemisia extract obtained in Example 2 1.5 (2) 1,3-butylene glycol 6.5 (3) Polyethylene glycol 1500 5.0 (4) Ethanol 5.5 (5) Potassium hydroxide 0.05 (6) Purified water 45.5 (7) 2-Hexyldecyl palmi 10.0 tate (8) Squalane 5.0 (9) Butylparaben 2 (10) Vitamin C 0.15 (11) Fragrance 0.05 (12) Purified water 19.9 (13) Carboxyvinyl polymer 0.2 (Production method) (7) to (11) were dissolved at 75 ° C. The product was added to (1) to (4) and (6) with stirring at 75 ° C. while stirring,
Further, (5), (12) and (13), which were dissolved by stirring at room temperature, were added and cooled while stirring to obtain a scalp treatment.

Example 9 Ointment (1) The mugwort extract obtained in Example 3 5.0 (2) Stearyl alcohol 18.0 (3) Mokuro 20.0 (4) Polyoxyethylene (10) 0.25 mono Oleic acid ester (5) Glycerin monostearic acid 0.25 ester (6) Vaseline 40.0 (7) Purified water 16.5 (Preparation method) Keep purified water at 70 ° C, and (Aqueous phase) Mix and dissolve at ゜ C. (Oil phase) The oil phase was added to the aqueous phase, uniformly emulsified with a homomixer, and then cooled to obtain an ointment.

Example 10 Toothpaste (1) Silicic anhydride 20.0 (2) Sorbit 50.0 (3) Carrageenan 0.5 (4) Artemisia extract obtained in Example 1 1.2 (5) Carboxy Methylcellulose 1.0 Sodium (6) Sodium lauryl sulfate 1.8 (7) Saccharin sodium 0.08 (8) Methyl parahydroxybenzoate 0.2 (9) Fragrance 0.9 (10) Purified water Residue I got

Example 11 Shampoo (1) Triethanolamine lauryl sulfate 15.0 (2) Coconut fatty acid monoethanolamide 5.0 (3) Artemisia extract obtained in Example 2 0.2 (4) Fragrance 0 9.9 (5) Purified water residue A shampoo was obtained by a conventional method.

Example 12 29.5% by weight of a gum base was placed in a chewing gum gum mixer, 10.0% by weight of starch syrup and a part of 60.0% by weight of powdered sugar were added.
Mix until uniform. After adding the remaining powdered sugar and further mixing and kneading, 0.5 wt% of the mugwort extract obtained in Example 1, an appropriate amount of a fragrance and a coloring agent are added, and finishing mixing is performed.
Then, after extruding with an extruder and passing through several rolling rollers, it is cooled to 5 to 15 ° C. After being cut to a prescribed size by a cutting roll and left at 20 to 25 ° C. for one day and night, a chewing gum was obtained.

The cosmetics, detergent compositions, oral compositions, and foods obtained from Examples 4 to 12 have no contact sensitizing properties, and can heal wounds, prevent rough skin, improve rough skin, and improve skin roughness. It was also effective in preventing itching, body odor and bad breath.

[0053]

The mugwort extract of the present invention has high stability because the causes of browning and precipitation have been removed, and has high safety because the sensitizing substance has also been removed. Therefore, various compositions containing the mugwort extract, that is, cosmetics, detergent compositions, oral compositions, foods, etc., have less coloring on clothes, and further provide wound healing, prevention of rough skin, improvement of rough skin, etc. It was also excellent in preventing skin itching, body odor and bad breath.

[Brief description of the drawings]

FIG. 1 is a gel permeation chromatogram of a protein having an average molecular weight of 15,000.

FIG. 2 is a gel permeation chromatogram of a heated extract in Example 1 of the present invention.

FIG. 3 is a gel permeation chromatogram of a mugwort extract obtained in Example 2 of the present invention.

FIG. 4 is a gel permeation chromatogram of a crude extract which has not been re-extracted with 70% ethanol in Example 2 of the present invention.

FIG. 5 is an infrared absorption spectrum of a mugwort extract of the present invention and an enzyme protein (protease) by a potassium bromide tablet method.

──────────────────────────────────────────────────の Continuation of front page (51) Int.Cl. ⁷ Identification symbol FI A61P 17/00 A61P 17/00 (56) References JP-A-2-96513 (JP, A) ALLERGY CLIN. IM MUNOL, VOL. 80, NO. 4, P 562-572, 1987 (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 35/78 A23L 1/30 A61K 7/00 A61P 17/00 BIOSIS (DIALOG) CA (STN) MEDLINE ( STN)

Claims (5)

(57) [Claims] A mugwort extract from which proteins having an average molecular weight of 15,000 have been substantially removed. 2. A cosmetic comprising the mugwort extract according to claim 1. 3. A cleaning composition comprising the mugwort extract according to claim 1. 4. An oral composition comprising the mugwort extract according to claim 1. 5. A food containing the mugwort extract according to claim 1.