JP3112983B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic product having a high moisturizing property, making the skin smooth and keeping the youthful skin.

[0002]

2. Description of the Related Art Lactobacillus acidophilus (Lacto
bacillus acidophilus belongs to the coryneform bacterium and is a bacterium belonging to the genus Lactobacillus.
ermobacterium), a subgenus, Gram-positive bacterium, catalase-negative, facultatively anaerobic, non-motile, non-spore-forming and sometimes linked, and is not pathogenic and is widely used as food. For example, yogurt, fermented lactic acid bacteria drink and the like.

[0003] On the other hand, it is already known to use a fermented metabolite of lactic acid bacteria as a raw material for cosmetics, but the type and production method of the lactic acid bacteria are different. Conventionally used strains are Streptococcus thermophilus (Streptococcus th.
ermophilus), cultivated in skim milk powder and glucose, sterilized,
Only the culture solution is used.

[0004]

SUMMARY OF THE INVENTION The object of the present invention is to provide a cosmetic product which has a high moisturizing property, smoothes the skin and keeps youthful skin, and which has high safety.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, obtained by finely pulverizing a culture solution and cells of Lactobacillus acidophilus, a kind of lactic acid bacterium. However, they found that they were very effective as cosmetics and completed the present invention. That is, the present invention

[0006] Lactobacillus acidophilus (Lac
tobacillus acidophilus) and its fermentation metabolites.

[0007] Lactobacillus acidophilus (Lac
Tobacillus acidophilus) and its fermentation metabolite are crushed and cosmetics containing glycerin.

[0008] The method of producing the active ingredient of the cosmetic of the present invention is based on Lactobacillus acidophilus.
dophilus) is cultured in a state in which other bacteria are not mixed, and after the culture, the cells are sufficiently pulverized together with the culture solution using ultrasonic waves, a French press, or a microfluidizer or a combination thereof. This solution is filtered through a membrane filter of 0.2 μm or less, or, depending on the purpose, an ultrafilter is used to remove substances having a molecular weight of 1,000 to 100,000 or more.

The aqueous solution thus obtained can be used by blending it in various dosage forms such as creams, lotions, packs, and serums. It has been found that when this aqueous solution, which is a feature of the present invention, is blended, the moisturizing properties are greatly improved, the moist feeling of the skin is increased, the fine wrinkles are reduced, and the effect of preventing rough skin is great. The cell activating effect was large, and safety was confirmed. It has also been found that other cosmetic ingredients used in combination are not particularly limited in terms of formulation.

[0010]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the present invention is not limited by these examples. (Production example) Lactobacillus acidophilus
llus acidophilus) (IFO 13951) was cultured at 37 ° C. in the medium shown in Table 1, and the cells and the fermented metabolite were treated twice with a microfluidizer at a primary pressure of 7 kg / cm ² , and then 0.1 μm. Filtered through a membrane filter. Its nitrogen content was 0.12% by weight (N).

[0011]

[Table 1]

(Example 1) Lotion A lactic acid bacterium fermentation metabolite of a production example 94.9% by weight Glycerin 5.0 {Methyl parahydroxybenzoate 0.1}

(Example 2) Cream A Squalane 20.0% by weight Jojoba oil 5.0 {Beeswax 5.0} Cetostearyl alcohol 2.0 {Glycerin monostearate 1.0} Sorbitan monostearate 2.0 B Purified water 46.9% by weight Polyoxyethylene (20E.O.) sorbitan monostearate 2.0〃 Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0〃 Glycerin 5.0〃 Methyl paraoxybenzoate 0.1% C Lactic acid bacterium fermentation metabolite of production example 10.0% by weight A and B were respectively weighed, heated to 70 ° C, and A was gradually added to B with stirring, cooled, and cooled to 50 ° C. Then, C was added thereto, and the mixture was cooled to 30 ° C. while stirring slowly.

(Example 3) In Example 2, glycerin was replaced with water. (Comparative Examples 1, 2, 3) Comparative examples 1, 2, and 3 were obtained by replacing the lactic acid bacteria fermentation metabolites of the production examples of Examples 1, 2, and 3 with water.

(Confirmation of effect) (1) Cell activation action test method Eagle was placed on a 6 cm diameter petri dish containing a cover crow.
MEM (20% fetal bovine serum) medium is dispensed in 5 ml portions.
Add 200,000 suspensions of JTC-17 cells and add 5% CO
_{2. The} cells were cultured at a temperature of 36 ° C. for 48 hours. Thereafter, the buffer PBS (-) (8.0 g of NaCl, 0.2 g of KCl 0.2
g, Na ₂ PO ₄ 1.15 g, KH ₂ PO ₄ 0.2
g) (buffer containing g), and then washed twice with Eagle MEM.
(Serum-free) 1 wt%, 2 wt%, 3 wt%, 5
A sample diluted to each concentration of wt% (a liquid prepared in the production example diluted to each concentration by MEM, the concentration is a liquid equivalent of the production example)
5 ml and 0.05 ml of fetal calf serum were added, and 5% CO ₂ , 3
After culturing at 6 ° C for 6 days, fixation and staining,
The following criteria were used for the determination.

Judgment Criteria [1] Judgment by Number of Cells Score -3: Almost 1.5 times as large as Control (substantially increased) -2: 4/3 of Control (increased to the extent that it can be seen with the naked eye) Slightly higher (slightly superior in staining density) 0: Same as Control 1: Slightly inferior to Control (slightly inferior in staining density) 2: 2/3 of Control (reduced to the naked eye) 3: Almost in Control Half (significant reduction) 4: 1/3 position of control (very few cells) 5: No cells are observed [2] Judgment by cell morphology Score 0: Normal image 1: Abnormal cells are slightly observed (Normal) 2: Abnormal cells are observed (normal cells are about 50 to 80%) 3: Many abnormal cells are observed (normal cells are about 20 to 50%) 4: Almost abnormal cells (normal) 5: Normal cells were observed Have

Results In the manner of display, the first number indicates the determination result based on the number of cells, and the number in parentheses indicates the determination result based on the cell morphology. A blank is prepared by adding the same amount of water in place of the sample. Table 2 shows the results
Shown in

[0018]

[Table 2] Blank 0 (3) The cell activation test was conducted under strict conditions, which resulted in rank 3. However, since the blank was also abnormal cell rank 3, there was no problem in skin safety.

(2) Moisturizing Test The following tests were conducted using the above-mentioned Example 1 and Comparative Example 1 as samples. Specimen 2 on 2 cm x 2 cm inside forearm of 20 women
Apply 0 μl uniformly. Apply 10 samples per person, but make sure that the same sample is not in the same position so as not to be affected by the application site. Before application, 20 minutes, 40 minutes, 6
After 0 minute and 80 minutes have elapsed, a high-frequency electric conductivity measuring device SKICON-200 (manufactured by IBS, wavelength 3.5 MH)
z, prog 2 mm). Table 3 shows the results.

[0020]

[Table 3]

(3) Usage test Thirty women were divided into two groups, the face was divided into left and right, one was used as an example, and the other was used as a comparative example, and was used at least once a day. In the comparative example, the lactic acid bacteria fermentation metabolite of the production example of the example was replaced with water. The criteria are very good for the example 3 Very good for the example 2 Somewhat better for the example 1 No difference 0 Somewhat better for the comparative example -1 Very good for the comparative example -2 comparison The example is much better. The results obtained by summing the -3 judgment values are shown in Table 4.

[0022]

[Table 4] The judgment value of Example 2 and Comparative Example 2 is smaller than that of Example 1 and Comparative Example 1 because Example 2 has a component having a good moisturizing effect other than the lactic acid bacteria fermentation metabolite. It is considered that the difference was smaller than that of Comparative Example 2 because a large amount was added.

[0023]

EFFECTS OF THE INVENTION The cosmetics of the present invention have excellent cell activating and moisturizing effects, smooth the skin and reduce fine wrinkles, as is clear from the results of the confirmation test, the moisturizing test and the use test of the cell activating effect. At the same time, it can prevent rough skin, has the effect of keeping youthful skin, and has no problem in safety.

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (2)

(57) [Claims] Claims: 1. Lactobacillus acidophilus (Lacto
A cosmetic comprising bacillus cells of Bacillus acidophilus) and a fermentation metabolite thereof. 2. Lactobacillus acidophilus (Lacto
(Bacillus acidophilus) and its fermentation metabolite are crushed and cosmetics containing glycerin.