JPH0240643B2 - - Google Patents

Description

[Detailed description of the invention]

TECHNICAL FIELD The present invention relates to a moisturizer for human skin that is used in cosmetics and the like. There are small amounts of metabolic products in epidermal cells on human skin, which have moderate moisturizing properties.
The skin is prevented from becoming too dry and remains supple. However, aged skin or skin that has just been washed with a detergent lacks the above-mentioned metabolites and therefore does not have a sufficient moisturizing effect, making the skin look dry and rough. If the natural moisturizing effect is not sufficient,
Artificially supplying moisturizing substances to the skin to prevent it from drying out has been practiced for a long time.
Usually, this is done by adding a moisturizing agent to lotions, milky lotions, creams, shampoo rinses, etc. Moisturizers used in cosmetics must have the property of retaining moisture without evaporating when the humidity in the air is low, and at the same time not absorbing moisture beyond a certain limit even when the humidity is high. . For example, glycerin, propylene glycol, ethylene glycol, etc. cannot be used because they are too hygroscopic and become sticky when the humidity is high. Furthermore, no matter how good the moisturizing performance is, substances that are harmful to human skin or have an unpleasant odor cannot be used. Typical examples of moisturizers that have conventionally been used to meet these requirements are royal jelly, Aqualyzer (trade name, a product whose main component is an ester of amino acids and reducing sugars), and fermented milk products. It is something. Among these, fermented milk products are made from whey, which is obtained by removing insoluble substances by centrifugation from the culture obtained by inoculating and culturing lactic acid bacteria in milk, and in addition to moisturizing properties, buffering action,
It has extremely favorable properties as a cosmetic ingredient, such as its ability to inhibit bacterial growth. The present invention provides a novel moisturizing agent that improves the moisturizing performance of the milk fermentation product as described above by improving the moisturizing agent, that is, a culture medium whose main ingredient is milk that has been made alkaline and treated with an alkaline proteolytic enzyme. The present invention provides a skin moisturizer made of a residual liquid obtained by inoculating and culturing lactic acid bacteria and separating insoluble substances from the resulting culture. The moisturizing agent according to the present invention can be used in cosmetics in the same way as conventional milk fermentation moisturizing agents, and cosmetics containing the same can be further improved in moisturizing performance and other overall cosmetic performance. Become something. Skimmed milk is most suitable as the raw material for producing the humectant according to the present invention, but whole milk may also be used. Of course, these may be reconstituted milk from powdered milk. When treating milk with proteolytic enzymes, it is desirable to adjust the milk solids concentration to about 4 to 15% and the pH to 8 to 10. Caustic soda, caustic potash, etc. are suitable as the alkali for pH adjustment. Some proteolytic enzymes are active on the acidic side, so if you just want to hydrolyze milk proteins, you can treat them with acid, but the treated product will have a strong amine odor,
Since this odor remains in the final product, treatment on the acidic side with acidic proteolytic enzymes is not preferred. Examples of alkaline proteolytic enzymes include those derived from microorganisms such as Aspergillus, Penicillium, Pseudomonas, Streptomyces, and Bacillus, such as Aspergillus oryzae, Bacillus subtilis, Bacillus thermoproteoliticus, etc. This is preferable because it is easy to obtain. Enzyme content is 50~ per gram of milk solids.
Use 2000 units and process at a temperature of 35 to 60°C for about 30 minutes to 4 hours to sufficiently hydrolyze milk proteins. Enzyme-treated milk may be used as a medium for culturing lactic acid bacteria, but it is desirable to add sugars and other substances effective in promoting the growth of lactic acid bacteria, such as glucose and sucrose. Cultivation of lactic acid bacteria using the enzyme-treated milk medium prepared as described above can be carried out in exactly the same manner as in the case of ordinary lactic acid bacteria beverage production. The lactic acid bacteria to be inoculated at this time are not particularly limited,
Any lactic acid bacteria may be used, such as Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, and Streptococcus thermophilus. Since the proteins in the culture have been hydrolyzed in advance by enzymatic treatment, the culture does not contain coagulated milk proteins unlike in the case of ordinary milk lactic acid bacteria cultures, and most of the insoluble substances are bacterial cells.
If this is separated using a centrifuge, the humectant of the present invention can be obtained as a supernatant. When used in cosmetics and other skin application liquids, the moisturizing agent of the present invention is desirably contained in an amount of about 0.05 to 1% by weight as a solid substance. Typical usage examples of the humectant of the present invention are shown below. In the examples below, the term "moisturizer" refers to the moisturizer A produced in the example below.
and numbers mean parts by weight. In the production of Example 1 and Example 2, which are emulsion type products, first a mixture of an aqueous component such as a humectant and a mixture of an oil component is prepared, and then the aqueous component and the oil component are mixed with stirring and poured into water. A conventional method of making an oil-type emulsion was adopted. Emulsification did not become difficult by using the humectant of the present invention. Example 1 Moisturizing cream moisturizer 20.0 Stearic acid 7.0 Stearyl alcohol 2.0 Paraffin 4.0 Liquid paraffin 4.0 Octyldodecyl myristate 4.0 Glyceryl monostearate 4.45 Polyoxyethylene cetyl ether 0.5 Preservative 0.3 1,3-butylene glycol 6.0 Sodium citrate 0.05 Disodium edetate 0.01 Fragrance Trace amount (Add purified water to make total volume 100.) Example 2 Skin milk stearic acid 2.0 Cetol 1.0 Hydrogenated oil 1.0 Refined lanolin 0.5 Liquid paraffin 5.0 Sorbitan monooleate 1.8 Potassium hydroxide 0.3 Allantoin 0.1 Preservative 0.2 Humectant 10.0 Polyoxyethylene sorbitan monolaurate (20E.O.) 0.3 Fragrance Trace amount (Add purified water to make the total volume 100.) Example 3 Moisture Lotion Ethanol 8.0 Fragrance Trace amount Citrus Sodium acid 1.3 Citric acid 0.5 Moisturizing agent 10.0 Allantoin 0.2 Glycerin 0.5 (Add purified water to make the total volume 100.) Example 4 Moistening pack Polyvinyl alcohol 13.0 Ethanol 10.0 Glycerin 2.0 Allantoin 0.1 Moisturizing agent 5.0 Fragrance Trace amount (purified water (The total amount is 100.) The present invention will be explained below with reference to Examples. Example: Dissolve 12 kg of skim milk powder in 300 ml of water, adjust the pH to 8.5 with caustic soda, then add 12 million units of alkaline proteolytic enzyme Proteoliquiface (Orientase 5N, Hankyu Kyoei Bussan Co., Ltd.) to 55 ml of water. When kept at ℃ for 1 hour, the milky white color disappeared and the liquid became transparent. After finishing the treatment, add 9 kg of glucose, adjust the temperature to 37℃, and then add lactic acid bacteria (Streptococcus thermophilus).
The starter was inoculated and cultured at that temperature for 48 hours. The obtained culture was centrifuged for 10 minutes using a centrifuge at 4000 rpm to remove a precipitate consisting mostly of bacterial cells to obtain a transparent moisturizing agent 2460 (hereinafter referred to as moisturizing agent A). Further, a moisturizer B was produced in the same manner as above except that the enzyme treatment was not performed. Next, a lotion with the following formulation was prepared using humectant A or B, and the feeling of use was compared and evaluated. Moisturizer (A or B) 20% Ethanol 8% Fragrance 0.2% Surfactant Micro-purified water 71.8% A panel of 53 housewives aged 30 to 50 in the Shonan area were used for the test, and both lotions were applied every other day. 3 days each
It was used for a total of 6 days. The order in which both samples were used was divided into two and the order differed for each group, so that rank effects could be ignored. The results are shown in Tables 1 and 2, and the product containing moisturizer A according to the present invention received an overwhelmingly higher evaluation.

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Claims (1)

[Claims] 1. A skin moisturizer consisting of the residual liquid obtained by inoculating and culturing lactic acid bacteria in a medium whose main component is milk made alkaline and treated with an alkaline proteolytic enzyme, and insoluble substances are separated from the resulting culture.