JP3056336B2 - Method and apparatus for removing dissolved oxygen from water for beverage or food production - Google Patents
Method and apparatus for removing dissolved oxygen from water for beverage or food productionInfo
- Publication number
- JP3056336B2 JP3056336B2 JP4249706A JP24970692A JP3056336B2 JP 3056336 B2 JP3056336 B2 JP 3056336B2 JP 4249706 A JP4249706 A JP 4249706A JP 24970692 A JP24970692 A JP 24970692A JP 3056336 B2 JP3056336 B2 JP 3056336B2
- Authority
- JP
- Japan
- Prior art keywords
- beverage
- dissolved oxygen
- water
- ascorbic acid
- dark
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- General Preparation And Processing Of Foods (AREA)
- Non-Alcoholic Beverages (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ジュース類やウーロン
茶のような各種飲料や、食品製造に使用する水（以下、
飲料又は食品製造用水という）の劣化を防止するための
飲料又は食品製造用水中の溶存酸素除去方法及び装置に
関するものである。BACKGROUND OF THE INVENTION The present invention relates to various drinks such as juices and oolong tea, and water used for food production (hereinafter referred to as "water").
The present invention relates to a method and an apparatus for removing dissolved oxygen in beverage or food production water for preventing deterioration of the beverage or food production water).
【０００２】[0002]
【従来の技術】ジュース類やウーロン茶のような各種飲
料は、カンやビン等に充填された後も徐々に劣化するこ
とが知られている。その主たる原因は飲料中に５〜10pp
m 程度含まれている溶存酸素であり、溶存酸素を0.1ppm
以下にまで低下させておけば劣化を防止することができ
る。このため、特開平3-236766号公報に示されるよう
に、飲料中にアスコルビン酸（ビタミンＣ）とこれを酸
化する酵素であるアスコルビン酸オキシダーゼとを添加
（但し、飲料中にアスコルビン酸を含有する場合はアス
コルビン酸の添加は不要）しておき、アスコルビン酸オ
キシダーゼによりアスコルビン酸を酸化させることによ
って飲料中より溶存酸素を除去する技術が提案されてい
る。2. Description of the Related Art Various beverages such as juices and oolong tea are known to gradually deteriorate even after being filled in cans and bottles. The main cause is 5-10pp in the beverage
m, which is about 0.1 ppm of dissolved oxygen.
Deterioration can be prevented if it is reduced to the following. Therefore, as shown in JP-A-3-236766, adding the ascorbic acid oxy Dark-peptidase, an enzyme which oxidizes to as ascorbic acid (vitamin C) in the beverage (however, ascorbic acid in the beverage a case containing the leave addition of ascorbic acid is required), and a technique for removing dissolved oxygen from the beverage has been proposed by oxidizing ascorbic acid by ascorbate San'o <br/> carboxymethyl Dark-peptidase.
【０００３】ところが、この方法では飲料中へ酵素を添
加するために最終的にこの酵素を除去するか、この酵素
自体を食品添加物として承認を受ける必要がある。また
この酵素は高価であり、上記のように飲料中に添加して
いたのでは経済的に引き合わない。このため、上記の方
法はいまだ実用化されるに至っていないのが実情であ
る。However, in this method, it is necessary to finally remove the enzyme in order to add the enzyme to the beverage, or to receive the approval of the enzyme itself as a food additive. In addition, this enzyme is expensive and is not economically viable if it is added to a beverage as described above. For this reason, the fact is that the above method has not yet been put to practical use.
【０００４】[0004]
【発明が解決しようとする課題】本発明は上記した従来
の問題点を解決して、飲料又は食品製造用水中へ酵素を
添加することなく飲料又は食品製造用水中の溶存酸素を
0.1ppm以下にまで低下させることができる飲料又は食品
製造用水中の溶存酸素除去方法及び装置を提供するため
になされたものである。DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned conventional problems and solves the problem of dissolving oxygen in beverage or food production water without adding enzymes to the beverage or food production water.
The present invention has been made to provide a method and an apparatus for removing dissolved oxygen in water for producing beverages or foods, which can be reduced to 0.1 ppm or less.
【０００５】[0005]
【課題を解決するための手段】上記の課題を解決するた
めになされた本発明の飲料又は食品製造用水中の溶存酸
素除去方法は、アスコルビン酸を含有させた飲料又は食
品製造用水を、セピオライトを主成分とするセラミック
担体にアスコルビン酸オキシダーゼを固定化したカラム
中に導き、カラム中で酸化反応を生じさせることにより
溶存酸素を低下させることを特徴とするものである。ま
た上記の課題を解決するためになされた本発明の飲料又
は食品製造用水中の溶存酸素除去装置は、セピオライト
を主成分とするセラミック担体の表面にアスコルビン酸
オキシダーゼを固定化した酸化触媒を、飲料又は食品製
造用水が導入されるカラム内に充填したことを特徴とす
るものである。Means for Solving the Problems To solve the above-mentioned problems, the method for removing dissolved oxygen in water for producing beverages or foods according to the present invention comprises dissolving water for producing beverages or foods containing ascorbic acid with sepiolite. Ceramic as the main component
Carrier leads to ascorbate oxy Dark-peptidase during immobilized column, is characterized in lowering the dissolved oxygen by causing an oxidation reaction in the column. Further, an apparatus for removing dissolved oxygen in water for beverage or food production according to the present invention made to solve the above-mentioned problem is a sepiolite.
Is characterized in that the immobilized oxidation catalyst ascorbic acid <br/> oxy Dark-zero on the surface of the ceramic support mainly was filled in a column of a beverage or food production water is introduced to .
【０００６】[0006]
【作用】本発明によれば、アスコルビン酸を含有させた
飲料又は食品製造用水をセピオ ライトを主成分とするセ
ラミック担体の表面にアスコルビン酸オキシダーゼを固
定化したカラム中に通過させるだけで、ごく短時間の間
に酸化反応を生じさせ、飲料又は食品製造用水中の溶存
酸素を低下させることができる。このときアスコルビン
酸オキシダーゼは担体に固定化されており、飲料又は食
品製造用水中に混入することはないので、酵素を飲料又
は食品製造用水中から除去したり、酵素を食品添加物と
して承認を受ける必要もない。またこの酵素は高価であ
るが、セピオライトを主成分とするセラミック担体に固
定化されているため流出することがなく、経済的にも十
分に実施可能である。According to the present invention, the beverage or food produced water was contained ascorbic acid as a main component Sepio write cell
In the surface of the ceramic carrier by passing ascorbate oxy Dark-peptidase during immobilized column causes an oxidation reaction in a very short period of time, it is possible to reduce the dissolved oxygen in the water for drinking or food preparation . In this case ascorbic acid oxy Dark-peptidase is immobilized on a carrier, so will not be mixed into the beverage or food production water, or remove the enzyme from the water for a beverage or food preparation, the enzyme as a food additive There is no need for approval. Although this enzyme is expensive, it does not flow out because it is immobilized on a ceramic carrier containing sepiolite as a main component, so that it can be implemented sufficiently economically.
【０００７】[0007]
【実施例】以下に本発明を図示の実施例に従って更に詳
細に説明する。図１は本発明の装置の一例を示すもの
で、１は恒温水が循環されるカラム、２はこのカラム１
内に充填された酸化触媒、３は飲料又は食品製造用水供
給用のポンプ、４は溶存酸素計、５はその記録計であ
る。酸化触媒２は、セラミック担体の表面にアスコルビ
ン酸オキシダーゼを固定化したものである。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in more detail with reference to the illustrated embodiments. FIG. 1 shows an example of the apparatus of the present invention, wherein 1 is a column through which constant temperature water is circulated, and 2 is a column 1
An oxidation catalyst filled in the pump, 3 is a pump for supplying water for producing beverage or food, 4 is a dissolved oxygen meter, and 5 is a recorder thereof. The oxidation catalyst 2 is obtained by immobilizing the ascorbic acid oxy Dark-zero on the surface of the ceramic support.
【０００８】まずアスコルビン酸オキシダーゼ（アスコ
ルビン酸を酸化する酵素の総称）としては、カボチャ、
キューリ等の植物由来のものと、菌由来のものとが知ら
れているが、植物由来のものは酸性領域では活性を失う
ため、ジュース類に対しては使用することができず、本
発明では菌由来のものを使用することが好ましい。また
菌由来のものは安定性があって固定化された状態で活性
が維持され易いといった利点がある。このような菌由来
のアスコルビン酸オキシダーゼとしては、前記した特開
平3-236766号公報に示されるように、従来からミクロセ
シウム・ベルカリア由来のもの、エーロバクター・エロ
ゲネス由来のもの、アクレモニウム由来のもの等が知ら
れている。[0008] As the first ascorbate oxy Dark-peptidase (generic name of enzymes which oxidize ascorbic acid), pumpkin,
Although those derived from plants such as cucumber and those derived from fungi are known, those derived from plants lose their activity in the acidic region and cannot be used for juices. It is preferable to use those derived from bacteria. In addition, those derived from bacteria have the advantage that they are stable and their activity is easily maintained in an immobilized state. As the ascorbic acid oxy Dark-peptidase from such bacteria, as shown in JP-A-3-236766 discloses that described above, those derived from micro cesium Berukaria conventionally derived from Eero aerogenes, Acremonium Origins are known.
【０００９】本発明では本発明者等が先に開発し、特公
平4-4014号として出願公告済みのセピオライトを主成分
とするセラミック担体を使用している。このセラミック
担体は酵素固定能力が優れるうえ、機械的強度に優れて
いるので高速で通液することができ、また高温に耐える
ので蒸気殺菌を容易に行える利点がある。なお、このよ
うなセラミック担体にアスコルビン酸オキシダーゼを固
定化する際には、アミノ酸を介在させた固定化方法を採
用することが好ましい。これによって酵素の活性を高く
維持することができ、しかも安定した固定化が可能で飲
料又は食品製造用水中へのアスコルビン酸オキシダーゼ
の混入をなくすることができる。In the present invention, a ceramic carrier mainly composed of sepiolite, which has been developed by the present inventors and has been published as Japanese Patent Publication No. 4-4014, has been used. This ceramic carrier has an advantage that it has excellent enzyme-fixing ability and mechanical strength, so that it can be passed at a high speed because of its high mechanical strength, and that it can withstand high temperatures to facilitate steam sterilization. Note that when fixing the ascorbate oxy Dark-peptidase such a ceramic carrier, it is preferable to employ a fixing method which is interposed amino acid. Consequently, it is possible to maintain a high activity of the enzyme, yet it is possible to eliminate contamination of the ascorbic acid-oxy Dark-peptidase to stable immobilization possible beverage or food production for water.
【００１０】上記したカラム１内に飲料又は食品製造用
水供給用のポンプ３から飲料又は食品製造用水を供給す
ると、アスコルビン酸１モルと酸素1/2 モルとが反応
し、デヒドロアスコルビン酸１モルと水１モルとが生成
される。この酸化反応により飲料又は食品製造用水中の
溶存酸素は急速に消費されるので、次のデータに示すよ
うに数分間で飲料又は食品製造用水中の溶存酸素は0.1p
pm以下にまで低下し、そのまま充填すれば充填後の期間
における飲料又は食品製造用水の劣化はほとんど防止で
きることとなる。When drink or food production water is supplied from the pump 3 for supplying drink or food production water into the column 1, 1 mol of ascorbic acid reacts with 1/2 mol of oxygen, and 1 mol of dehydroascorbic acid reacts with 1 mol of dehydroascorbic acid. One mole of water is formed. Since the dissolved oxygen in the beverage or food production water is rapidly consumed by this oxidation reaction, the dissolved oxygen in the beverage or food production water is reduced to 0.1p in a few minutes as shown in the following data.
pm or less, and if the filling is performed as it is, deterioration of the drink or food production water during the period after the filling can be almost prevented.
【００１１】本発明の効果を確認するため、次の実験を
行った。まず活性が270U/mg の粉末アスコルビン酸オキ
シダーゼ10mgを10ccの水に溶かし、その中にセピオライ
トを主成分とするセラミック担体１ｇを室温にて一昼夜
静置し、固定化した。この結果、酵素活性が15.2U/g-担
体の酸化触媒を得た。なお、1U (ユニット) は１分間に
１μモル（0.176mg)のアスコルビン酸を酸化する酵素量
を意味する。The following experiment was conducted to confirm the effects of the present invention. First activity was dissolved powder ascorbic acid Oki <br/> sheet Dark-peptidase 10mg of 270 U / mg of water 10 cc, and overnight standing at room temperature ceramic carrier 1g mainly composed of sepiolite therein, immobilized did. As a result, an oxidation catalyst having an enzyme activity of 15.2 U / g-carrier was obtained. Here, 1 U (unit) means the amount of an enzyme that oxidizes 1 μmol (0.176 mg) of ascorbic acid per minute.
【００１２】この酸化触媒を充填したカラム１内に、入
口溶存酸素濃度が10〜11ppm の緩衝溶液 (pH 4) を操作
温度30℃、反応時間７分（カラム内における滞留時間７
分）の条件で供給し、出口溶存酸素濃度を溶存酸素計４
で連続的に測定した。なおこの緩衝溶液中には予めアス
コルビン酸を添加し、5000mg/Lを含有させておく。この
実験を200 時間継続した結果を図１のグラフに示す。図
１から明らかなように、反応時間がわずか７分であって
も緩衝溶液の出口溶存酸素濃度は0.01〜0.3ppmに維持さ
れていることが分かる。Into the column 1 filled with the oxidation catalyst, a buffer solution (pH 4) having a dissolved oxygen concentration of 10 to 11 ppm at the inlet is operated at 30 ° C. for a reaction time of 7 minutes (residence time of 7 minutes in the column).
Min), and measure the dissolved oxygen concentration at the outlet with a dissolved oxygen
Was measured continuously. In addition, ascorbic acid is added to this buffer solution in advance to contain 5000 mg / L. The result of continuing this experiment for 200 hours is shown in the graph of FIG. As can be seen from FIG. 1, the dissolved oxygen concentration at the outlet of the buffer solution is maintained at 0.01 to 0.3 ppm even when the reaction time is only 7 minutes.
【００１３】次に入口溶存酸素濃度が２〜６ppm のオレ
ンジジュース(pH 3 〜 4) を同じ条件で処理した。オレ
ンジジュースには310mg/L のアスコルビン酸が含有され
ているため、添加はしなかった。その結果を図２のグラ
フに示す。この図から明らかなように、オレンジジュー
スについてもその出口溶存酸素濃度は0.1ppm以下に維持
されていることが分かる。Next, orange juice (pH 3 to 4) having an inlet dissolved oxygen concentration of 2 to 6 ppm was treated under the same conditions. Orange juice contained 310 mg / L ascorbic acid and was not added. The results are shown in the graph of FIG. As is clear from this figure, it can be seen that the concentration of dissolved oxygen at the outlet of the orange juice is maintained at 0.1 ppm or less.
【００１４】なお、入口溶存酸素濃度が４〜６ppm のウ
ーロン茶、煎茶についても同様の試験を行ったが、出口
溶存酸素濃度はウーロン茶は0.3 〜2.0ppm、煎茶は0.02
ppm以下となることが確認できた。ウーロン茶はアスコ
ルビン酸の含有量が少ないため、出口溶存酸素濃度の値
がやや高いが、アスコルビン酸を添加するか反応時間を
延長することにより容易に0.1ppm以下とすることができ
る。The same test was conducted for oolong tea and sencha having an inlet dissolved oxygen concentration of 4 to 6 ppm, but the outlet dissolved oxygen concentration was 0.3 to 2.0 ppm for oolong tea and 0.02 for sencha.
It could be confirmed that it was less than ppm. Since oolong tea has a low content of ascorbic acid, the value of the dissolved oxygen concentration at the outlet is somewhat high, but it can be easily reduced to 0.1 ppm or less by adding ascorbic acid or extending the reaction time.
【００１５】[0015]
【発明の効果】以上に説明したように、本発明によれば
ごく短時間の処理により飲料又は食品製造用水中の溶存
酸素濃度を0.1ppm程度にまで低下させることができ、飲
料又は食品製造用水の劣化を防止することができる。し
かも本発明によればアスコルビン酸オキシダーゼが飲料
又は食品製造用水中へ混入することがなく、またその消
費量はごくわずかであるため、経済的である。As described above, according to the present invention, the concentration of dissolved oxygen in beverage or food production water can be reduced to about 0.1 ppm by a very short treatment, and the beverage or food production water can be reduced. Degradation can be prevented. Moreover without ascorbate oxy Dark-peptidase according to the present invention may be mixed into the beverage or food production for water, also the consumption thereof since it is negligible, it is economical.
【図１】本発明の装置を示すフローシートである。FIG. 1 is a flow sheet showing the apparatus of the present invention.
【図２】緩衝溶液からの溶存酸素除去実験の結果を示す
グラフである。FIG. 2 is a graph showing the results of an experiment for removing dissolved oxygen from a buffer solution.
【図３】オレンジジュースからの溶存酸素除去実験の結
果を示すグラフである。FIG. 3 is a graph showing the results of an experiment for removing dissolved oxygen from orange juice.
１ カラム ２ 酸化触媒 ３ ポンプ ４ 溶存酸素計 ５ 記録計 1 column 2 oxidation catalyst 3 pump 4 dissolved oxygen meter 5 recorder
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ Ａ２３Ｌ 3/3589 Ａ２３Ｌ 2/34 (56)参考文献 特開 平３−236766（ＪＰ，Ａ) 特開 昭58−56686（ＪＰ，Ａ) 特開 平４−183393（ＪＰ，Ａ) 特開 平２−252669（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) A23L 3/3463 - 3/3589 A23L 2/42 - 2/84 ──────────────────────────────────────────────────続 き Continuation of the front page (51) Int.Cl. 7 Identification symbol FI A23L 3/3589 A23L 2/34 (56) References JP-A-3-236766 (JP, A) JP-A-58-56686 (JP) JP-A-4-183393 (JP, A) JP-A-2-252669 (JP, A) (58) Fields investigated (Int. Cl. 7 , DB name) A23L 3/3463-3/3589 A23L 2/42-2/84
Claims (4)
品製造用水を、セピオライトを主成分とするセラミック
担体にアスコルビン酸オキシダーゼを固定化したカラム
中に導き、カラム中で酸化反応を生じさせることにより
溶存酸素を低下させることを特徴とする飲料又は食品製
造用水中の溶存酸素除去方法。1. A method for producing a beverage or food containing ascorbic acid comprising a ceramic containing sepiolite as a main component.
Carrier leads to ascorbate oxy Dark-peptidase during immobilized column, dissolved oxygen removal method in water for a beverage or food preparation, characterized in that to reduce the dissolved oxygen by causing an oxidation reaction in the column.
担体の表面にアスコルビン酸オキシダーゼを固定化した
酸化触媒を、飲料又は食品製造用水が導入されるカラム
内に充填したことを特徴とする飲料又は食品製造用水中
の溶存酸素除去装置。Wherein the oxidation catalyst immobilized ascorbic acid oxy Dark-zero on the surface of the ceramic <br/> carrier mainly composed of sepiolite, that filled in the column beverage or food production water is introduced A device for removing dissolved oxygen in water for producing beverages or foods.
ものである請求項２記載の飲料又は食品製造用水中の溶
存酸素除去装置。3. The apparatus according to claim 2, wherein the ascorbate oxidase is derived from a bacterium.
スコルビン酸オキシダーゼを固定化した請求項２記載の
飲料又は食品製造用水中の溶存酸素除去装置。Wherein the surface of the carrier, the beverage or food preparation for the water dissolved oxygen reducing apparatus according to claim 2, wherein the immobilized ascorbic acid oxy Dark-peptidase with intervening amino group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4249706A JP3056336B2 (en) | 1992-09-18 | 1992-09-18 | Method and apparatus for removing dissolved oxygen from water for beverage or food production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4249706A JP3056336B2 (en) | 1992-09-18 | 1992-09-18 | Method and apparatus for removing dissolved oxygen from water for beverage or food production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0698737A JPH0698737A (en) | 1994-04-12 |
| JP3056336B2 true JP3056336B2 (en) | 2000-06-26 |
Family
ID=17196994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4249706A Expired - Lifetime JP3056336B2 (en) | 1992-09-18 | 1992-09-18 | Method and apparatus for removing dissolved oxygen from water for beverage or food production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3056336B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4753902B2 (en) * | 2007-03-09 | 2011-08-24 | 株式会社常盤産業 | Organic oxygen absorber |
-
1992
- 1992-09-18 JP JP4249706A patent/JP3056336B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0698737A (en) | 1994-04-12 |
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