JP3054079B2 - Method for producing sesame extract mainly containing water-soluble protein containing trace metals in high concentration - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the versatile use of sesame defatted lees, which is hardly used except as feed or fertilizer. That is, although the present invention has a low lysine content compared to soybean extracted lees that are being applied and developed as a vegetable protein, the amino acid composition of the protein of sesame defatted lees is very rich, Compared to methionine and other 8 amino acids such as cysteine, this is a method for producing sesame extract that is highly useful as a new protein resource.
The present invention relates to a method for producing a sesame extract in which trace metals such as zinc, iron, copper, and manganese, which are nutrients necessary for enzyme synthesis in the human body, are extracted at a high concentration in a bound peptide state having a good bioabsorption rate.

[0002]

2. Description of the Related Art Conventionally, a method for producing sesame extract has been considered to easily extract proteins contained at a high concentration in sesame defatted meal in a high yield.
Solvent extraction methods and a number of methods have been disclosed. Also in the enzymatic degradation process, US Pat. No. 2,232,052
A typical example is disclosed in the above-mentioned publication.

[0003] However, these methods are simply a method of obtaining a water-soluble extract containing a high concentration of proteins from oil-extracted lees mainly containing soybean lees, or a method using sesame defatted lees. This is a method for obtaining a high yield by purifying a protein, and does not use and remove all useful portions contained in the sesame defatted meal for humans.

[0004]

The present invention focuses not only on the protein of such conventional sesame defatted lees, but also on a useful portion other than the protein contained in sesame, that is, selenium, zinc, iron and copper.・ Sesame extract containing a high concentration of trace metals, such as manganese, which are nutrients required for enzyme synthesis in the human body in a highly bioabsorbable binding peptide, is a type of industrial waste that is hardly used except for animal feed and fertilizers. It is an object of the present invention to provide a method for producing sesame defatted lees.

[0005]

In order to solve the above problems, in the present invention, sesame defatted lees are put into hot water, and
Heating at a temperature of not less than ° C. can prevent spoilage of microorganisms adhering to sesame defatted lees during the proteolysis reaction, and the enzymatic decomposition of the protein proceeds promptly without an unpleasant odor. In the sterilization step performed by heating to 75 ° C. or higher, sesame defatted lees put in hot water is p
It is also possible to perform the adjustment after adjusting so that H becomes 2.0 or more and less than 5.5.

[0006] In the proteolytic reaction, sesame defatted lees are cooled to 20 to 70 ° C after heat treatment, and peptidases which are resistant to synthetic or natural acidic regions, such as acidic regions, under the condition of pH 2.0 or more and less than 5.5. The reaction is carried out with a protease that is resistant to Bacillus subtilis or Aspergillus. A sesame extract mainly composed of a water-soluble protein containing a high concentration of a trace amount of a metal-binding peptide by subjecting a heat treatment at 75 ° C. or higher after the decomposition reaction to separating and concentrating the obtained decomposition solution and drying the concentrated solution into a dry powder. Can be obtained.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail.

[0008] In the present invention, varieties and places of production of sesame are not limited, but it is desirable to use one having a large amount of protein and minerals. However, it is most desirable to use large quantities of feed and fertilizer.
It is also possible to use any state of raw sesame, roasted pressed extract cake, pressed extract cake, solvent extracted cake, and supercritical extracted cake. For example, pulverized sesame roast and pressed extract cake is put into hot water at 75 ° C or higher, and the microorganisms adhering to the sesame roast and press extract cake are sterilized at 75 ° C or more for 30 minutes or more to be enzymatically decomposed. Prevent corruption and deterioration of time. Then, an appropriate acid, preferably hydrochloric acid, malic acid, citric acid, tartaric acid, lactic acid, acetic acid, fumaric acid, phthalic acid, vinegar, etc., which can be used for food, is added to the mixture to adjust the pH to 2.0 or more and less than 5.5, preferably Is p
The pH is adjusted to 2.5 to 3.5 and the degradation is performed at 20 to 70 ° C. by adding a synthetic or natural acid domain resistant peptidase such as a protease that produces Bacillus subtilis or a koji mold. In this step, the substantial molecular weight: about 100 to 4,000,
Thus, a group of binding peptides with good bioabsorption of trace metals, such as selenium, zinc, iron, copper, and manganese, which are nutrients necessary for enzyme synthesis in the human body can be obtained.

The decomposition time is 0.5 to 6.0 hours, preferably about 3.0 hours. After alkali treatment of the prior art or after neutral or alkaline enzymatic decomposition and water-extracted residue, etc., it is also possible to extract by performing decomposition on the acidic side again by the method of the present invention. is there.

[0010] Immediately after the decomposition of the decomposition solution obtained in this way, 7
The temperature is raised to 5 ° C. or higher to completely inactivate the proteolytic enzyme and prevent the quality from deteriorating.

The decomposition solution thus obtained is separated into an extract layer, an oil layer and an undecomposed layer by a filter press, a centrifuge or the like, and then the extract layer is filtered and concentrated under reduced pressure at 60 ° C. or lower.

The sesame extract obtained according to the present invention has a substantial molecular weight of about 100 to 4,000,
Zinc, iron, copper, manganese and other nutrients required for enzymatic synthesis in the human body, which are mainly composed of bioabsorbable binding peptides of trace metals. Sesame extract containing a high concentration can be obtained.

[0013]

Embodiments of the present invention will be described below.

600 kg of sesame roasted pressed extract cake is put into 6 tons of hot water at 95 to 100 ° C. in a reaction vessel equipped with a stirrer,
Hold at 0 ° C for 30 minutes.

Next, the temperature is lowered to 50 ° C. and the pH is reduced with vinegar.
The pH was adjusted to 3.0, 1.5 kg of Bacillus subtilis-produced protease was added, and the mixture was reacted for 3.0 hours.

Then, the temperature was raised to 80 ° C. to inactivate the enzyme.

This reaction solution was separated into an extract layer, an oil layer and an undecomposed layer by a centrifuge, and the extract layer was filtered and concentrated under reduced pressure at 60 ° C. or lower to obtain a sesame extract.

Table 1 shows the results of component analysis of the sesame extract obtained by the method of the present invention and the conventional method.

[0019]

[Table 1]

From Table 1, it is clear that the method according to the present invention contains a high amount of trace metals, especially metal-binding peptides such as iron, copper, zinc, manganese and selenium.

As a result of molecular weight analysis of this sesame extract, molecular weight: 100 or less (9%), around 340 (10%), 7
Around 94 (7%), around 1,200-3,000 (72
%).

The results of the method of the present invention and the results of amino acid analysis of the commercially available enzyme decomposed products of soy protein are shown in Table 2.

[0023]

[Table 2]

From Table 2, it is apparent that the protein obtained by the method of the present invention is a protein resource in which the lysine content and the sulfur-containing amino acid content are well-balanced as compared with soybean by being converted into sesame extract.

[0025]

According to the present invention, SOD (superoxide dismutase) is a trace metal that promotes protein synthesis in the human body, promotes the formation of hormones such as insulin, and maintains blood acid-alkali balance. And GOP (glutathione peroxidase) is a coenzyme of active oxygen scavenging enzyme. If this enzyme is deficient, active oxygen cannot be scavenged,
It causes various diseases including cancer.
Even if the trace element is taken into the body as it is, its absorbency is poor, but according to the present invention, the molecular weight is 100 to 4,
It can have a high absorption rate in the small intestine having about 000 peptide bonds, and is highly useful as a supplement for sick, elderly, children, etc., whose absorption power is low. The sesame extract of the present invention is soluble in water and contains a large amount of protein. Therefore, for the sick, it can be dissolved in water or hot water, soybean milk, milk, etc. and used as an oral or tube food. For elderly people, children and the like, they can be used by adding to dairy products, processed soy foods, processed animal foods, and eat and drink.

Further, since it can be easily processed into liquids, powders, granules, etc., it can be applied to health foods such as beverages, tablets, and granulating agents. Can be ingested.

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Claims (2)

(57) [Claims] 1. A method for producing a sesame extract mainly comprising a water-soluble protein containing a trace metal in a high concentration, comprising the following steps 1 to 5. 1. Put sesame defatted lees into hot water of 5 parts by weight or more,
A process of heating to above ℃ to sterilize. 2. Cool to 20-70 ° C. and pH 2.0 with any acid
The pH is adjusted to less than 5.5, and the acid-domain resistant protease is reacted for 0.5 to 6.0 hours to decompose into peptide amino acids having a large amount of trace metal bonds, and heated to 75 ° C or higher to activate the enzyme. Process. 3. A step of separating and concentrating the obtained decomposition solution. 4. A step of dry-concentrating the concentrated liquid. 2. The method according to claim 1, wherein
The method for producing a sesame extract according to claim 1, wherein the sterilization step performed by heating to 75 ° C or more is performed after adjusting the pH to 2.0 or more and less than 5.5 with an optional acid.