CN104957580B - Preparation method for milk powder enzymatic hydrolysates and application of prepared products - Google Patents

Preparation method for milk powder enzymatic hydrolysates and application of prepared products Download PDF

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CN104957580B
CN104957580B CN201510421121.8A CN201510421121A CN104957580B CN 104957580 B CN104957580 B CN 104957580B CN 201510421121 A CN201510421121 A CN 201510421121A CN 104957580 B CN104957580 B CN 104957580B
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milk powder
zymolyte
milk
parts
preparation
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CN104957580A (en
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经晶
张贡博
张树林
易封萍
黄梅桂
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TIANNING FLAVOR (JIANGSU) CO Ltd
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TIANNING FLAVOR (JIANGSU) CO Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Dairy Products (AREA)

Abstract

The invention discloses a preparation method for milk powder enzymatic hydrolysates and application of the prepared products. The method comprises the following steps: by taking milk powder as a raw material, separating out effective components through enzymatic hydrolysis, centrifuging through a centrifugal machine to remove visible solid matters to obtain clear, transparent, soft and full milk characteristic enzymatic hydrolysate A, and carrying out enzymatic hydrolysis on turbid solution again to obtain a lactic-acid milk characteristic enzymatic hydrolysate B. The milk powder enzymatic hydrolysates are used in an essence, thus greatly improving the quality of the essence and reducing the production cost.

Description

A kind of preparation method of milk powder zymolyte and the application of prepared product
Technical field
The invention belongs to food additive field, the preparation method and prepared product of specifically a kind of milk powder zymolyte Application.
Technical background
Milk flavor application in the market is quite varied, but milk essence is formed with compound allotment, shadow Ring the quality of essence.
The content of the invention
For the problem for overcoming prior art to exist, it is an object of the invention to provide a kind of preparation method of milk powder zymolyte And the application of prepared product, the method obtains the soft full milk feature zymolyte of clear using zymolysis technique, muddy Turbid liquid Jing zymolysis techniques obtain again yoghourt feature zymolyte, for essence in, greatly improve the quality of essence, reduce Production cost.
The purpose of the present invention is achieved through the following technical solutions:
A kind of preparation method of milk powder zymolyte, it is characterised in that:The method is isolated with milk powder as raw material by enzymolysis Effective component, then the visible solid content of Jing centrifuges removal, obtains the soft full milk feature of clear Zymolyte A, turbid solution digests obtain yoghourt feature zymolyte B again, specific as follows:
1)Select whole milk powder;A portion whole milk powder is taken, is 1 by material water g/mL ratios:3---1:10 ratio adds distilled water, PH6.0---6.5 is adjusted with the NaOH solution of 1mol/L, heat up inactivation, 85 DEG C of bath temperature -- 95 DEG C, 10-30 points of time Clock;
2)Milk powder Jing enzymolysis processings, then be centrifuged, clear liquor is taken, obtain milk feature zymolyte A;Enzymatic hydrolysis condition For:0.3 part of papain, at 50 DEG C -65 DEG C, the response time is in 3---6 hours, intensification enzyme denaturing, water-bath temperature for reaction temperature 95 DEG C of degree -- 98 DEG C, the discharging centrifugation of 20-30 minutes time, centrifuge speed is at 1000-3000 rev/min;
3)Above-mentioned dirty solution, by solid-liquid ratio 1:1 adds propylene glycol solvent, adds distilled water to adjust pH7.0 while stirring; Heat up inactivation, 95 DEG C of bath temperature -- and 100 DEG C, 10-20 minutes time;Enzymolysis processing again, then be centrifuged, take clarification Liquid, obtains yoghourt feature zymolyte B;Enzymatic hydrolysis condition is:0.1 part of flavor protease, reaction temperature at 50 DEG C -55 DEG C, instead In 4---8 hours between seasonable, intensification enzyme denaturing, 95 DEG C of bath temperature -- 100 DEG C, 10-20 minutes time, discharging centrifugation, centrifuge Rotating speed is at 3000-5000 rev/min.
In the present invention, milk powder is New Zealand's whole milk powder, and material-water ratio is 1:3, PH is 6.5,95 DEG C of bath temperature, the time 30 Minute.
A kind of preparation method of milk powder zymolyte obtains application of the product in terms of sweet taste essence is prepared, and the product for obtaining is protected Stay original fruit head fragrant, for essence in, greatly improve the quality of essence.
During milk powder Jing enzymolysis processings;Milk powder can be digested according to the condition of different enzymolysis, different enzymatic hydrolysate are entered Row precipitation experiments, record time and well-illuminated degree, and the first evaluation of product fragrance, are determined optimal by optimal combination condition Hydrolase.Optimum enzymolysis condition is:For the first time enzymolysis selects 0.3 part of papain, reaction temperature at 57 DEG C, the response time At 4 hours, intensification enzyme denaturing, 95 DEG C of bath temperature, 30 minutes time discharging was centrifuged, and centrifuge speed is at 3000 revs/min;Now Clear liquor is optimal;Second enzymolysis selects 0.1 part of flavor protease, and reaction temperature at 55 DEG C, at 4 hours, heat up by the response time Enzyme denaturing, 95 DEG C of bath temperature, 20 minutes time, discharging centrifugation, centrifuge speed at 5000 revs/min, now most preferably.
The present invention isolates effective component with milk powder as raw material by enzymolysis, and then Jing centrifuges remove visible Solid content, obtain the soft full milk feature zymolyte A of clear, turbid solution digests obtain yoghourt feature again Zymolyte B.The milk powder zymolyte that the present invention is prepared using enzyme process has fragrance mouthfeel true to nature good, while remaining the head of itself Perfume (or spice), greatly improves the quality of milk essence.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
Product embodiments
1)50 portions of New Zealand's whole milk powders are weighed, is 1 by material water g/mL ratios:3 ratio adds distilled water, NaOH solution (1mol/L) pH6.5 is adjusted, heat up inactivation, 95 DEG C of bath temperature, 30 minutes time, is cooled to 57 DEG C, add papain 0.3 part, the response time, intensification enzyme denaturing, 95 DEG C of bath temperature, 30 minutes time, discharging centrifugation, centrifuge speed existed at 4 hours 3000 revs/min;Obtain 80 parts of supernatant and be milk powder zymolyte A.
2)Dirty solution presses solid-liquid ratio(1:1)Propylene glycol solvent is added, adds distilled water to adjust pH7.0 or so while stirring. Heat up inactivation, 98 DEG C of bath temperature, 20 minutes time;Turbid solution enzymolysis processing again, then be centrifuged, obtain clear liquor; Enzymatic hydrolysis condition is:0.1 part of flavor protease, reaction temperature at 55 DEG C, the response time at 4 hours, intensification enzyme denaturing, bath temperature 95 DEG C, 20 minutes time, discharging centrifugation, centrifuge speed is at 5000 revs/min;Obtain 20 parts of supernatant and be zymolyte B.
Application Example
Application of the milk powder zymolyte in water solublity milk-taste essence:4 parts of 2-heptanone, 2 parts of methyl n-heptyl ketone, 8 parts of diacetyl, butanoic acid 1 Part, 25 parts of milk powder zymolyte A, 2 parts of butyl acetate, 3 parts of acetoin part, 50 parts of ethanol, 5 parts of distilled water.Firmly shake up, place Ripening, obtains condensed milk fragrance essence.
Embodiment 2
The preparation method of milk powder zymolyte is same as Example 1.
Application of the milk powder zymolyte in oil-soluble milk-taste essence:5 parts of arbricolin, 2 parts of vanillin, 0.1 part of octanal, diacetyl 0.05 part, 0.1 part of butanoic acid, 0.1 part of maltol, 0.2 part of fourth position dodecalactone, 0.2 part of second position dodecalactone, 55 parts of vegetable oil, 37.25 parts of milk powder zymolyte B.Firmly shake up, place ripening, obtain coconut palm milk-taste essence.
Embodiment 3
The preparation method of milk powder zymolyte is same as Example 2.
Application of the milk powder zymolyte in Water/oil dual-purpose milk-taste essence:4 parts of ethyl n-butyrate., 2 parts of capric acid, fourth position dodecalactone 5 parts, 3 parts of vanillin, 5 parts of isopropylformic acid., 2 parts of mannitol, 7 parts of butanoic acid, 5 parts of diacetyl, 10 parts of milk powder zymolyte A, milk powder enzyme 3 parts of solution thing B, 47 parts of Propylene Glycol firmly shakes up, and places ripening, obtains milk-taste essence.
Embodiment 4
The preparation method of milk powder zymolyte is identical with embodiment 3
Application of the milk powder zymolyte in emulsifying essence:Foreign minister's liquid;6 parts of glyceryl monostearate, 1 part of sodium benzoate, lemon Lemon acid is appropriate, 58 parts of distilled water.Interior phase liquid;14 parts of milk powder zymolyte A, 0.8 part of 2-heptanone, 0.5 part of phenol of butyl butyryl lactate 1 part, 10 parts of methyl n-heptyl ketone, 1 part of 1-OCOL, 7 parts of butanoic acid, 0.7 part of methyl cinnamate.Stirring and dissolving, homogenizing places ripening. Obtain cheese taste essence.
The milk powder zymolyte that the various embodiments described above preparation method is obtained is added in food flavor, enhances the fresh of essence Sense, at utmost remains the original fragrance of milk taste, and the quality to improving food flavor plays positive role.

Claims (2)

1. a kind of preparation method of milk powder zymolyte, it is characterised in that:The method has been isolated with milk powder as raw material by enzymolysis The component of effect, then the visible solid content of Jing centrifuges removal, obtains the soft full milk feature enzyme of clear Solution thing A, turbid solution digests obtain yoghourt feature zymolyte B again, specific as follows:
1)50 portions of New Zealand's whole milk powders are weighed, is 1 by material water g/mL ratios:3 ratio adds distilled water, with 1mol/L's NaOH solution adjusts pH6.5, and heat up inactivation, 95 DEG C of bath temperature, 30 minutes time, is cooled to 57 DEG C, adds papain 0.3 part, the response time, intensification enzyme denaturing, 95 DEG C of bath temperature, 30 minutes time, discharging centrifugation, centrifuge speed existed at 4 hours 3000 revs/min;Obtain 80 parts of supernatant and be milk powder zymolyte A;
2)Dirty solution after centrifugation presses solid-liquid ratio 1:1 adds propylene glycol solvent, adds distilled water to adjust pH7.0 while stirring;Rise Temperature inactivation, 98 DEG C of bath temperature, 20 minutes time;Turbid solution enzymolysis processing again, then be centrifuged, obtain clear liquor;Enzyme Solution condition is:0.1 part of flavor protease, reaction temperature at 55 DEG C, the response time at 4 hours, intensification enzyme denaturing, bath temperature 95 DEG C, 20 minutes time, discharging centrifugation, centrifuge speed is at 5000 revs/min;Obtain 20 parts of supernatant and be zymolyte B.
2. the preparation method of milk powder zymolyte described in a kind of claim 1 obtains application of the product in terms of sweet taste essence is prepared.
CN201510421121.8A 2015-07-17 2015-07-17 Preparation method for milk powder enzymatic hydrolysates and application of prepared products Active CN104957580B (en)

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