JP2916041B2 - Algae and plant cell culture method and culture light source device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for culturing algae such as chlorella and spirulina, and plant cells.

[0002]

2. Description of the Related Art Conventionally, the following four main methods for culturing algae and plant cells using an artificial light source are known. First, as shown in FIG.
There is a method of irradiating the culture solution in the culture tank 1 with light by using a lamp 2 installed outside. Second, as shown in FIG. 3, there is a method of irradiating the culture solution in the culture tank 3 with light by a lamp 4 installed on one or both sides of the flat and thin culture tank 3.

[0003] Third, as shown in FIG. 4, a lamp 6 housed in an inner tube 5 a provided in a cylindrical culture tank 5 irradiates light from the inside of the culture tank 5 to the culture solution in the culture tank 5. There is a method of irradiation. Fourth, as shown in FIG. 5 and FIG.
The light from a light source 9 such as a xenon lamp is guided to an optical fiber 8 disposed on the inner wall of the optical fiber 8, and the guided light is radiated from the peripheral surface of the optical fiber 8 into the inside of the culture tank 7. There is a method of irradiating the culture solution in 7 with light.

In selecting an artificial light source, points such as lamp efficiency, lifespan, price, light wavelength of the light source are suitable for cell growth, and dimming are possible. When evaluated, the fluorescent lamp is the best. Therefore, among the above four methods, the first to third methods shown in FIGS. 2 to 4 can be said to be preferable methods in that a fluorescent lamp can be used as a light source.

[0005]

However, in the first and second methods shown in FIG. 2 and FIG.
There is a limit on the surface area of the culture tanks 1 and 3 to which the light is irradiated, and since the reflection of light occurs on the surfaces of the culture tanks 1 and 3, of the total amount of light emitted from the lamps 2 and 4, Only a part can be introduced into the culture tanks 1 and 3. Therefore, the illuminance of the culture solution cannot be increased as compared with the light amounts of the lamps 2 and 4, and the growth rate of algae and the like in the culture tanks 1 and 3 becomes slow. There was a defect.

[0006] In the third method shown in FIG. 4, almost all of the light emitted from the lamp 6 is introduced into the culture tank 5, although the light is slightly reflected on the surface of the inner cylinder 5 a. be able to. However, the capacity of the culture tank 5 is reduced by the amount corresponding to the formation of the inner cylinder 5a. For example, when a large number of light sources must be installed for scale-up, the amount of the culture solution must be reduced accordingly. However, there was a problem that the culture could not be performed efficiently.

Further, since the fluorescent lamp is of an AC lighting type, if the fluorescent lamp is housed in the inner cylinder 5a of the culture tank 5 shown in FIG. An induced current is generated in the culture solution, and the current leaks from the metal part of the culture device that comes into contact with the culture solution to the ground, and this leaked high-frequency current affects the cultured cells, which slows down the cell growth rate. there were.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-described problem. When a fluorescent lamp is used as a light source for culturing algae or plant cells, the present invention maintains a high light-guiding efficiency while preventing current leakage, and proliferates. It is an object of the present invention to provide a method for culturing algae and plant cells, which allows efficient cultivation without slowing down the speed.

[0009]

To achieve the above object, the present invention provides a method for culturing algae and plant cells in a culture solution by irradiating a culture solution in a culture tank with light of a fluorescent lamp. Wherein at least a part of the fluorescent lamp is immersed in the culture solution, and the fluorescent lamp is turned on by a direct current. Also, the present invention
A culture light source device for culturing algae and plant cells in a culture solution, a DC lighting type fluorescent lamp at least a part of which is immersed in the culture solution, and a DC light source for lighting the fluorescent lamp. And a ballast.

[0010]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram illustrating the configuration of a culture apparatus according to one embodiment of the present invention.

In FIG. 1, reference numeral 10 denotes a culture tank formed of a transparent material such as acrylic, and the inside thereof is filled with a culture solution in which a cyanobacterium (Spirulina Platensis, hereinafter referred to as Spirulina) is immersed. . A drainage pipe 22 is connected to the upper end 11 of the culture tank 10, and the drainage pipe 22 communicates with an upper end of a culture liquid reflux pipe 21. On the other hand, a liquid supply pipe 23 is connected to the lower end 12 side wall of the culture tank 10, and the liquid supply pipe 23 is connected to a lower end of the reflux pipe 21 via a magnet pump 24. For this reason, when the magnet pump 24 is operated, the culture solution is supplied to the culture tank 1.
From inside 0, drain pipe 22, reflux pipe 21, magnet pump 2
4 and again through the feed pipe 23 into the culture tank 10. An exhaust pipe 25 extends upward from the upper end of the reflux pipe 21.

Further, a porous diffuser plate 13 is provided at the lower end 12 of the culture tank 10, and a mass flow controller 33 is connected via an air filter 34. The mass flow controller 33 includes an air compressor 3
1 and a carbon dioxide gas cylinder 32 are connected to each other.
Air into which the carbon dioxide gas is mixed is supplied as sterile air into the culture tank 10 via 3.

Further, a stainless steel tube 42 connected to a humidity controller 41 is provided inside the culture tank 10. The stainless steel tube 42 is cooled or heated by the humidity controller 41, so that the culture tank is heated. The temperature of the culture solution in 10 can be adjusted to an arbitrary temperature.

A one-sided DC lighting fluorescent lamp 50 is inserted from the upper end 11 of the culture tank 10 to the inside thereof, and is immersed in the culture solution in the culture tank 10. A connector 62 is connected to a base portion of the fluorescent lamp 50 exposed from the upper end 11 to the outside. The connector 62 is provided at the tip of the wiring cord 61, and a DC current from a DC stabilizer 60 connected to a commercial power supply (not shown) is supplied to the fluorescent lamp 50 via the wiring cord 61 and the connector 62. Is done.

When culturing Spirulina with the culturing apparatus of the present embodiment having the above-described configuration, the temperature of the culture solution is adjusted by the humidity controller 41, and the magnet pump 24 is operated to cultivate the cultivation in the reflux tube 21. The liquid is discharged from the liquid supply pipe 23 into the culture tank 10. At the same time, aseptic air mixed with carbon dioxide is pumped from the air diffuser 13 provided at the lower end 11 of the culture tank 10 into the culture solution.

Thus, the culture solution in the culture tank 10 is vigorously agitated by the culture solution discharged from the liquid supply pipe 23 and the sterile air which is intersected with the culture solution and pressure-fed from the diffusing plate 13. Further, the culture solution in the culture tank 10 is discharged to the drainage pipe 22 by an air lift effect due to the aseptic air pumping,
The culture solution discharged to the drainage pipe 22 is returned to the culture tank 1 via the reflux pipe 21, the magnet pump 24, and the liquid supply pipe 23.
Reflux into 0.

Then, in this state, the fluorescent lamp 50 emits light by the DC current from the DC stabilizer 60, and the culture solution in the culture tank 10 is irradiated with light, whereby spirulina in the culture solution is grown.

As described above, in the culture apparatus of the present embodiment, since the fluorescent lamp 50 for irradiating the culture solution with light is immersed in the culture solution in the culture tank 10, the culture solution is not reduced so much. Can be efficiently irradiated with light. In addition, since the fluorescent lamp 50 is of a DC lighting type in which the DC lamp is turned on by a DC current from the DC stabilizer 60, an induced current is generated in the culture solution, and the current leaks from the metal part of the culture device in contact with the culture solution to the ground. Can be prevented. Therefore,
The cultivation of Spirulina in the culture solution can be performed efficiently by eliminating the influence of the leak current on the culture of Spirulina and preventing the growth rate from slowing down.

In this embodiment, the culture solution is supplied to the culture tank 10,
Drain pipe 22, reflux pipe 21, magnet pump 24, liquid supply pipe 23, and reflux to culture tank 10,
The means for stirring the culture solution in the culture tank 10 is not limited to this, but a stirring rod or the like is disposed in the culture tank 10 and the culture solution is stirred by the stirring rod. The configuration for stirring the culture solution may be omitted.

Next, a spirulina culturing experiment was performed using the culturing apparatus of the present embodiment having the above configuration and a culturing apparatus using an inverter stabilizer instead of the DC stabilizer 60 in the culturing apparatus. The results will be described.

The various conditions in this experiment are as follows. Culture tank 10: Acrylic, inner diameter 11c
m, length 90 cm, reflux tube 21: made of acrylic, 4 cm inside diameter,
Length 150cm Drainage pipe 22: Acrylic, 4cm inside diameter,
Length 15cm Supply tube 23: Stainless steel, inner diameter 1c
m, length 4 cm Stainless steel tube 42: 1 cmφ Magnet pump 24: Rated output 15 W Fluorescent lamp 50: 96 W type x 2 lamp Algae used: Spirulina Culture temperature: 35 ° C Illumination: 40 klux (culture tank 10)
Sterile air: air with a carbon dioxide gas concentration of 0.4% Aseptic air pressure from air diffuser 13: 3.00 l / min. Medium used: SOT medium

The composition of the SOT medium in 100 ml is shown in Table 1.

[0023]

[Table 1]

Furthermore, it shows the composition of A ₅ metal mixture in 100 ml of Table 2.

[0025]

[Table 2]

Under the above conditions, in this experiment, cultivation of Spirulina was performed for 3 days in the culture apparatus of the present example and the culture apparatus using an inverter stabilizer instead of the DC stabilizer 60 in the culture apparatus. Was done. For sampling, 10 ml of the culture solution was sampled every day, suction-filtered with a membrane filter having a pore size of 1 μm, dried, and the dry weight of Spirulina was measured. Table 3 shows the measurement results of the current flowing from the metal part in contact with the culture solution to the ground in each culture device at that time.

[0027]

[Table 3]

As shown in Table 3, in the culture apparatus of the present embodiment using the DC ballast 60 as the stabilizer of the fluorescent lamp, the current leakage is clearly improved as compared with the culture apparatus using the inverter ballast. You can see that it is done. As shown in Table 4 showing the results of cultivation of Spirulina by each culture device, the production amount of Spirulina by the culture device of the present example was 16.2 g larger than that of the culture device using an inverter stabilizer, and Was found to be able to improve the growth efficiency.

[0029]

[Table 4]

[0030]

As described above, according to the present invention, when culturing algae and plant cells in the culture solution by irradiating the culture solution in the culture tank with light from a fluorescent lamp, At least a portion is immersed in the culture solution, and the fluorescent lamp is turned on by a direct current, so that high light guide efficiency is maintained while preventing current leakage through the culture solution, and algae and plant cells Efficient culture can be performed without slowing the growth rate.

[Brief description of the drawings]

FIG. 1 is a diagram illustrating the configuration of a culture apparatus according to an embodiment of the present invention.

FIG. 2 is a configuration explanatory view showing an example of a conventional culture device.

FIG. 3 is a configuration explanatory view showing another example of a conventional culture device.

FIG. 4 is a configuration explanatory view showing another example of a conventional culture device.

FIG. 5 is a configuration explanatory view showing another example of a conventional culture device.

FIG. 6 is a configuration explanatory view showing another example of a conventional culture device.

[Explanation of symbols]

 10 Culture tank 50 Fluorescent lamp 60 DC stabilizer

Claims (2)

(57) [Claims] 1. A culture method for culturing algae and plant cells in a culture solution by irradiating a culture solution in a culture tank with light of a fluorescent lamp, wherein at least a part of the fluorescent lamp is in the culture solution. Wherein the fluorescent lamp is turned on by a direct current. A method for culturing algae and plant cells. 2. A culture light source device for culturing algae and plant cells in a culture solution, comprising: a direct current lighting type fluorescent lamp at least a part of which is immersed in the culture solution; A light source device for culture, comprising: a DC ballast for lighting.