JP2779071B2 - Biochemical automatic analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention comprises a plurality of analysis units. Each analysis unit introduces a sample and a reagent into a reaction vessel, stirs the mixture, measures the absorbance of the reacted reaction solution and the rate of change in absorbance to determine the concentration. The present invention relates to a biochemical automatic analyzer that performs required analysis.

[0002]

2. Description of the Related Art In a biochemical automatic analyzer, a sample for introducing a sample and a reagent into a reaction vessel, a reagent introducing process, a reaction process for stirring and reacting the introduced sample and reagent, and a measurement for measuring a reacted reaction solution are performed. The analysis of the sample sample is completed by performing the process, and the discharging and washing process of discharging and washing the measured reaction solution. Generally, a large number of the reaction vessels are arranged at equal intervals on the same circumference of the rotating body so that such analysis can be performed continuously, and a reaction system for intermittently moving the rotating body is provided. is there. Recently, a multi-channel automatic chemical analyzer in which a plurality of the reaction systems are provided in parallel so as to simultaneously analyze several items at a time is widely used.

[0003] In this multi-channel automatic biochemical analyzer, the analysis process described above in each reaction system, that is, the sample,
A method in which a series of analysis cycles of a reagent introduction process, a stirring reaction process, a measurement process, a discharge process, and a washing process are simultaneously performed in the same phase at the same time, is proposed by the present applicant (for example, JP-A-1-51325)
As described above, there is a method of performing analysis by shifting the phase between the analysis systems. In the former simultaneous analysis method, a sample amount required for the number of channels is aspirated at a time, and after dispensing it for each channel, a sample can be simultaneously supplied to each channel. Therefore, there is an advantage that the sample supply means for supplying a sample to each channel can be shared. Also,
The latter shift analysis method can eliminate the play of the reaction system caused by the number of analysis items, which is a disadvantage of the simultaneous analysis method.

[0004]

However, when the same analysis item is measured by a plurality of analysis units, a difference in detection sensitivity occurs between the analysis units, and it is necessary to correct the difference.

FIG. 6 is a diagram for explaining a conventional method for correcting a difference in detection sensitivity for each analysis unit. The conventional correction is that the absorbance or the rate of change of absorbance corresponding to the concentration D is A
Analyzing unit OD _a B analysis unit has When there was a OD _b, the correction of the measured value seeking correction coefficient from the relationship between the 'b relative to' a passing through the origin. That is, conventionally, a fixed type of correction coefficient is determined for each analysis unit from the proportional relationship between the concentration and the absorbance or the rate of change in absorbance, and correction is performed using the correction coefficient. However, such correction using a single correction coefficient cannot correct the data difference due to the difference between the analysis units, and thus has a problem that the reliability of the measurement accuracy cannot be improved.
This is because biochemical automatic analyzers generate data differences due to differences in colorimeters as well as differences in analysis units such as sample volume and reagent volume. There is no calibration curve like a, b. Therefore,
In order to completely correct these differences, it is necessary to determine several correction factors for each analysis unit.
This requires a lot of trouble.

An object of the present invention is to solve the above-mentioned problems, and an object of the present invention is to provide a biochemical automatic analyzer which can easily correct the difference between each analysis unit with a small correction coefficient and effort. Things.

The present invention solves the above-mentioned problems, and in a biochemical automatic analyzer having a plurality of analysis units, the analysis accuracy can be improved by correcting each analysis unit with a small amount of measurement and processing. The purpose is to do so.

[0008]

For this purpose, the present invention comprises a plurality of analysis units, in which each analysis unit introduces a sample and a reagent into a reaction vessel, stirs and measures the absorbance and the rate of change in absorbance of the reacted reaction solution. In a biochemical automatic analyzer that determines and analyzes the concentration or activity value, the reagent blank correction coefficient and the reaction correction coefficient are calculated from the reagent blank value and the reaction measurement value of each unit obtained by measuring the reagent blank and measuring the reaction. Means for determining, storing, and setting the values of the sample, and determining the reagent blank value of another unit using the reagent blank correction coefficient from the reagent blank value obtained by one or more analysis units when measuring the sample, and measuring the reaction value of the sample. , A concentration or an activity value is obtained using a reagent blank value, a reagent blank correction coefficient, and a reaction correction coefficient.

[0009]

In the automatic biochemical analyzer of the present invention, a reagent blank correction coefficient is determined for each analysis unit in advance to determine a reagent blank correction coefficient, and a reaction measurement is performed to determine and store a reaction correction coefficient. Since the other reagent blank values are obtained from the reagent blank values obtained by one or more analysis units using the reagent blank correction coefficient, it is possible to obtain one reagent blank value only once a day, for example, once a day prior to the reaction measurement of the sample. , Other blank values can be determined. Therefore, the concentration value or the activity value can be corrected by the reaction measurement of the sample using the blank value, the reagent blank correction coefficient, and the reaction correction coefficient, so that the difference in the detection sensitivity by the analysis unit can be corrected with a simple operation. be able to.

[0010]

Embodiments will be described below with reference to the drawings.
FIG. 1 is a view showing one embodiment of the automatic biochemical analyzer according to the present invention, and FIG. 2 is a view for explaining calculation of data by the automatic biochemical analyzer.

In FIG. 1, an analysis unit 1 has a plurality of analysis units 2-1, 2-2,... And can process the same analysis item in parallel. Data operation unit 3
.. Perform control of each analysis unit 2-1, 2-2,... Of the analysis section 1 and analysis processing of analysis data. The storage section 4 stores reagent blank values, standard values,
It stores a reaction correction coefficient and a reagent blank correction coefficient. That is, the data calculation unit 3 corrects a difference in detection sensitivity of each analysis unit using the reagent blank value, the standard value, and the correction coefficient stored in the storage unit 4, and analyzes the sample.

In the automatic biochemical analyzer according to the present invention, the absorbance actually measured or the rate of change in absorbance (actually measured value)
Density value D _X calculated from OD _X is as follows. _{D X = D S × (OD} X -OD R) ÷ OD S (1) where, D _S is the concentration of the standard (concentration of standard)
OD _R is absorbance or rate of absorbance change by only reagents was measured without a sample (reagent blank value), OD _S is the reagent blank value from the standard value (absorbance or rate of absorbance change was measured standards with known concentrations (Subtracted value). FIG. 2 shows these relationships by a calibration curve.

The present invention firstly provides a reagent blank correction coefficient,
The reaction correction coefficient is calculated in advance for each of the analysis units 2-1, 2-2,... Of the analysis unit 1 and stored in the storage unit 4, and any analysis is performed prior to the actual measurement of the sample. In the unit, the reagent blank value of each analysis unit is calculated from the reagent blank value and the standard value obtained by actual measurement, and the concentration value is obtained from the reagent blank value, the standard value, and the reaction correction coefficient. The outline of the processing will be described below.

FIG. 3 is a diagram for explaining the calculation of the correction coefficient, FIG. 4 is a diagram for explaining the flow of the calculation process of the correction coefficient, and FIG. 5 is a diagram for explaining the flow of the process at the time of analysis. is there.

First, a method of obtaining the correction coefficients of the analysis units 2-1 and 2-2 will be described. As shown in FIG. 4, a reagent blank measurement is performed to calculate a reagent blank correction coefficient, and the reagent blank value and the reagent blank correction coefficient are stored in the storage unit. The correction coefficient α _R of the reagent blank is obtained by measuring the measured values OD _R1 (1B REAGENT BLANK in FIG. 5) and OD under the conditions for measuring a normal reagent blank in both the analysis units 2-1 and 2-2.
_{When R2} (2B REAGENTBLANK in FIG. 5) is obtained, α _R = OD _R1 ÷ OD _R2 (2) is obtained, and the analysis unit 2-1 is obtained as a reagent blank value.
OD _R1 is stored.

Subsequently, the actual measurement values OD _X1 and OD _X2 are obtained under the conditions for measuring normal reactions in the analysis units _2-1 and 2-2, and the reaction correction coefficient β is calculated. The reaction correction coefficient β is obtained from β = (OD _X1 −OD _R1 ) ÷ (OD _X2 −OD _R1 ÷ α _R ) (3). In the above equation, (OD _X1 −OD _R1 ) is the 1B reaction value in FIG. 5, and (OD _X2 −OD _R1 ÷ α _R ) is the 2B reaction value in FIG. In this case, the sample may be a standard substance or a normal specimen.

As described above, first, the analysis unit 2
After the 2-2 reagent blank correction coefficient α _R and the reaction correction coefficient β for −1 are obtained and stored in the storage unit, the processing shown in FIG. In the measurement of a sample, first, a reagent blank value OD _R ′ is obtained using one of the analysis units prior to the actual measurement. This is usually one day
It is executed in the morning.

[0018] For example the analysis unit 2-1 measured value by measuring the reagent blank by od _R1 'when got, the measured value od _R1' is left as it is a reagent blank value OD _R 'a, the analysis unit 2 over In the case where the reagent blank is measured in accordance with Step ₂ and the actual measurement value od _R2 'is obtained, a value (od _R2 ' × α _R ) obtained by multiplying the actual measurement value by the correction coefficient α _R of the reagent blank is used as the reagent blank value OD _R '. And

[0019] Similarly, 'regard to the analysis unit Found od _R1 by measuring the standard substance by 2-1' Standard value OD _S when got, the measured value od reagent blank values from _R1 'OD _R '(Od _R1 ' -O
D _R to ') the standard value OD _S'. However, the standard substance was measured by the analysis unit 2-2, and the measured value od was measured.
_R2 'when give _{in, (od R2' -OD R '} ÷ α R) ×
β, that is, (actual value−reagent blank value ÷ reagent blank correction coefficient) × reaction correction coefficient is defined as a standard value OD _S ′.

As described above, the reagent blank correction coefficient α
_R, reaction correction coefficient beta, reagent blank value OD _R ', Standard value OD _S' when are stored in all the storage unit 4, can calculate the concentration value by the sample measurement in each analysis unit 2-1,2-2 Become. That is, the measured value od _X1 of analysis units 2 - 1, for _{D S × (od X1 -OD R} ') ÷ OD S' (4) Found od _X2 analysis unit 2-2 is D _S × ( od _X2 −OD _R ′ ÷ α _R ) × β ÷ OD _S ′ (5)

As described above, the reagent blank correction coefficient α _R ,
Since the reaction correction coefficient β is specific to the analysis unit, it may be fixed after one measurement. However, these against a reagent blank value OD _R ', Standard value OD _S', since there is an element that varies daily, by clearing them at the end of measurement, for example 1 day, processing as shown in FIG. 4 When starting the measurement of the day, that is, in the morning, the reagent blank value OD _R ′,
The measurement of the standard value OD _S 'is performed.

It should be noted that the present invention is not limited to the above embodiment, and various modifications are possible. For example, in the above-described embodiment, a comparative measurement method with a standard substance has been described, but an absolute measurement without using a standard value can be similarly corrected. Further, it goes without saying that a method of performing a plurality of measurements in the measurement of the correction coefficient and performing a statistical calculation on the measurement and storing a more correct value may be employed. In this case, various methods such as an average value, a median value, and an average value according to the Hoffman method can be used as the statistical calculation method.

Further, in the measurement of the reagent blank value and the standard value, a plurality of measured values measured by a plurality of analysis units may be statistically processed to store more correct values.

Further, in the above embodiment, the common reagent blank value OD _R ′ is stored in the storage unit 4, and the sample is calculated from the common reagent blank value OD _R ′ and the reagent blank correction coefficient α _R obtained in advance. Although calculated density value or activity value by performing a calculation each time (5) of the measurement, (5) and stores the calculated value in advance and a calculation of the OD _R '÷ α _R in the storage unit 4 in the formula When calculating the concentration value or activity value,
The equation (5) may be calculated using the stored calculation value, and in this case, the calculation equation is simplified and is practically advantageous.

[0025]

As is apparent from the above description, according to the present invention, a reaction correction coefficient and a reagent blank correction coefficient are calculated for each analysis unit, and these correction coefficients and one or more analysis units are calculated. Since the reagent blank value and the standard value of each analysis unit are set from the measured values, the concentration correction of the analysis item of each analysis unit can be performed only by the measurement with one or a plurality of analysis units.

[Brief description of the drawings]

FIG. 1 is a view showing one embodiment of an automatic biochemical analyzer according to the present invention.

FIG. 2 is a diagram for explaining calculation of data of a biochemical automatic analyzer.

FIG. 3 is a diagram for explaining calculation of a correction coefficient.

FIG. 4 is a diagram illustrating a flow of a calculation process of a correction coefficient.

FIG. 5 is a diagram for explaining the flow of processing at the time of analysis.

FIG. 6 is a diagram for explaining a conventional method for correcting a difference in detection sensitivity for each analysis unit.

[Explanation of symbols]

1. Analytical unit, 2-1 2-2,... Analytical unit, 3.
Data operation unit, 4 ... storage unit

──────────────────────────────────────────────────続 き Continuation of the front page (72) Inventor Susumu Saito 3-1-2 Musashino Akishima, Tokyo Japan Japan Electronics Co., Ltd. (58) Field surveyed (Int. Cl. ⁶ , DB name) G01N 35/00- 35/10

Claims (1)

(57) [Claims] 1. A system comprising a plurality of analysis units, wherein each analysis unit introduces a sample and a reagent into a reaction vessel, stirs, measures absorbance and a change in absorbance of the reacted reaction solution to determine a concentration or an activity value, and performs analysis. In the biochemical automatic analyzer to be performed, means for obtaining and storing and setting the reagent blank correction coefficient and the reaction correction coefficient from the reagent blank value and the reaction measurement value of each unit obtained by performing the measurement of the reagent blank and the measurement of the reaction are provided. In measuring a sample, the reagent blank value of another unit is obtained using a reagent blank correction factor from a reagent blank value obtained by one or more analysis units, and a reagent blank value, a reagent blank correction factor,
An automatic biochemical analyzer wherein a concentration or an activity value is determined using a reaction correction coefficient.