JP2726500C - - Google Patents
Info
- Publication number
- JP2726500C JP2726500C JP2726500C JP 2726500 C JP2726500 C JP 2726500C JP 2726500 C JP2726500 C JP 2726500C
- Authority
- JP
- Japan
- Prior art keywords
- igg
- sample
- human igg
- agalactosyl
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090001095 Immunoglobulin G Proteins 0.000 claims description 67
- 102000004851 Immunoglobulin G Human genes 0.000 claims description 67
- 108010046488 agalactosyl IGG Proteins 0.000 claims description 26
- 239000000020 Nitrocellulose Substances 0.000 claims description 15
- 229920001220 nitrocellulos Polymers 0.000 claims description 15
- 108010075569 Rheumatoid Factor Proteins 0.000 claims description 14
- 239000012528 membrane Substances 0.000 claims description 14
- 239000000032 diagnostic agent Substances 0.000 claims description 12
- 102000004169 proteins and genes Human genes 0.000 claims description 12
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 206010072736 Rheumatic disease Diseases 0.000 claims description 10
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 9
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 9
- 101700028499 LECG Proteins 0.000 claims description 8
- 239000002523 lectin Substances 0.000 claims description 8
- 101700036312 CONA Proteins 0.000 claims description 7
- 101700021338 LEC Proteins 0.000 claims description 7
- 101700077545 LECC Proteins 0.000 claims description 7
- 101700063913 LECT Proteins 0.000 claims description 7
- 101710034340 Os04g0173800 Proteins 0.000 claims description 7
- 101700036391 lecA Proteins 0.000 claims description 7
- 101710026914 AAG Proteins 0.000 claims description 5
- 101700048515 LEC1 Proteins 0.000 claims description 5
- 101700046135 LEC2 Proteins 0.000 claims description 5
- 101700028593 LECH Proteins 0.000 claims description 5
- 239000000910 agglutinin Substances 0.000 claims description 5
- 108010062580 Concanavalin A Proteins 0.000 claims description 3
- 101700018328 ccdB Proteins 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- AIXAANGOTKPUOY-UHFFFAOYSA-N Carbachol Chemical compound [Cl-].C[N+](C)(C)CCOC(N)=O AIXAANGOTKPUOY-UHFFFAOYSA-N 0.000 claims description 2
- 101710015954 HVA1 Proteins 0.000 claims description 2
- 101700065814 LEA2 Proteins 0.000 claims description 2
- 240000000528 Ricinus communis Species 0.000 claims description 2
- 235000004443 Ricinus communis Nutrition 0.000 claims description 2
- 108010030727 lens intermediate filament proteins Proteins 0.000 claims description 2
- 101700001016 mbhA Proteins 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 40
- 210000002966 Serum Anatomy 0.000 description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 229940072417 Peroxidase Drugs 0.000 description 15
- 102000003992 Peroxidases Human genes 0.000 description 15
- 108090000437 Peroxidases Proteins 0.000 description 15
- 230000000903 blocking Effects 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 14
- 239000000243 solution Substances 0.000 description 14
- 229940088598 Enzyme Drugs 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 235000018102 proteins Nutrition 0.000 description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 10
- GZCGUPFRVQAUEE-KCDKBNATSA-N D-(+)-Galactose Natural products OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-KCDKBNATSA-N 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 8
- 238000005406 washing Methods 0.000 description 7
- 108090001090 Lectins Proteins 0.000 description 6
- 102000004856 Lectins Human genes 0.000 description 6
- 102000005348 Neuraminidase Human genes 0.000 description 6
- 108010006232 Neuraminidase Proteins 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- 230000000552 rheumatic Effects 0.000 description 5
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 5
- 206010039073 Rheumatoid arthritis Diseases 0.000 description 4
- 239000012148 binding buffer Substances 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108010010803 Gelatin Proteins 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 102000004965 antibodies Human genes 0.000 description 3
- 108090001123 antibodies Proteins 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229920001542 oligosaccharide Polymers 0.000 description 3
- 150000002482 oligosaccharides Polymers 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 102000002464 Galactosidases Human genes 0.000 description 2
- 108010093031 Galactosidases Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K [O-]P([O-])([O-])=O Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 108010014507 erythroagglutinating phytohemagglutinin Proteins 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 102000005614 monoclonal antibodies Human genes 0.000 description 2
- 108010045030 monoclonal antibodies Proteins 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 125000002306 tributylsilyl group Chemical group C(CCC)[Si](CCCC)(CCCC)* 0.000 description 2
- IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 description 1
- WONRDHPFOHAWOG-UHFFFAOYSA-N 2-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=C(Cl)C=CC2=C1 WONRDHPFOHAWOG-UHFFFAOYSA-N 0.000 description 1
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 1
- 101710027066 ALB Proteins 0.000 description 1
- 240000005781 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 206010003246 Arthritis Diseases 0.000 description 1
- 229960001230 Asparagine Drugs 0.000 description 1
- 108090000206 Autoantibodies Proteins 0.000 description 1
- 102000003852 Autoantibodies Human genes 0.000 description 1
- 229940039227 DIAGNOSTIC AGENTS Drugs 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000219726 Griffonia simplicifolia Species 0.000 description 1
- 229940098197 Human Immunoglobulin G Drugs 0.000 description 1
- 229940027941 Immunoglobulin G Drugs 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 235000010666 Lens esculenta Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000219822 Macrotyloma axillare Species 0.000 description 1
- 229910020936 NaC Inorganic materials 0.000 description 1
- 229940074730 OPHTHAMOLOGIC DIAGNOSTIC AGENTS Drugs 0.000 description 1
- 235000001504 Pferdebohne Nutrition 0.000 description 1
- 240000005158 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000007643 Phytolacca americana Species 0.000 description 1
- 235000009074 Phytolacca americana Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000010500 Vigna catjang Nutrition 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000038129 antigens Human genes 0.000 description 1
- 108091007172 antigens Proteins 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 235000020127 ayran Nutrition 0.000 description 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 150000001720 carbohydrates Chemical group 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drugs Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 125000002519 galactosyl group Chemical group C1([C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001264 neutralization Effects 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- -1 pH 7.4) Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- YZCKVEUIGOORGS-NJFSPNSNSA-N tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 235000021307 wheat Nutrition 0.000 description 1
Family
ID=
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