JP2722670B2 - Food and drink for preventing hypertension and method for producing the same - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food and drink for preventing hypertension comprising a component having a high inhibitory activity on asia tensin converting enzyme as an active ingredient, and a method for producing the same.

2. Description of the Related Art As is well known, renin-angiotensin-based substances play a central role in essential hypertension, which is said to account for 90% or more of all hypertension, and in renal vascular hypertension caused by renal artery stenosis. Is acting. This renin-
In the angiotensin system, angiotensin converting enzyme (hereinafter abbreviated as ACE) activates angiotensin I
Works on its C-terminal peptide (histidine-leucine)
To produce angiotensin II, which exhibits a strong pressor action, and increases blood pressure.

In view of the above, many attempts have been made to inhibit ACE based on the above mechanism to lower blood pressure to prevent or treat hypertension, and a component having ACE inhibitory activity has attracted attention. Specifically, while several kinds of drugs having ACE inhibitory activity have been chemically synthesized, natural products of casein degradation products (JP-A-58-109425 and JP-A-59-44323),
Black (Japanese Patent Publication No. 62-120320), tea ingredients (Agricultural Journal,
61 , (7), 803 (1987)), etc., are reported to contain an ACE inhibitory active ingredient. In addition, it has been reported that a wide range of foods contain active ingredients when ACE inhibitory activity is measured for foods taken daily (Suzuki et al., Agricultural Chemistry, 57 , (11), 1143 (1983)).

Problems to be Solved by the Invention However, when trying to efficiently ingest ACE inhibitory active ingredients as food or drink, chemically synthesized products having ACE inhibitory activity are not preferable to be added to food or drink from the viewpoint of safety, Natural products containing an ACE inhibitory active ingredient must be consumed in large quantities at a time in order to obtain the expected effect. Problems may arise and affect taste. Furthermore, the casein hydrolyzate and kurosu are difficult to purify because all of the active ingredients having their ACE inhibitory activity are peptide substances having a specific structure.
In addition to the increase in cost, the active ingredient may be decomposed in the body by digestive enzymes (peptidases) and may not exert sufficient ACE inhibitory activity. On the other hand, AC in tea components
Although the main component of the E-inhibiting active ingredient is catechin, it is expensive to use as a food or drink because the raw material tea is relatively expensive, and there are problems of astringency and bitterness peculiar to catechin.

Although cutting the ACE-inhibiting active ingredient as food or drink is effective in preventing hypertension, there are many problems in effectively ingesting the ACE-inhibiting active ingredient as food or drink.

The present invention has been made in view of the above circumstances, and provides a hypertensive-preventing food and drink, which is effective for preventing hypertension, comprising a component having a high ACE inhibitory activity as an active ingredient, and a method for producing the same. Aim.

Means and Actions for Solving the Problems The present inventors have conducted intensive studies to achieve the above object, and as a result, it has been known that they contain an ACE inhibitory active ingredient, solanaceae, myrtaceae, urushi family and papaya family. One or two or more selected from plants belonging to, preferably tomato, guava, mango or papaya fruit at 20 ℃ relative dielectric constant γ 17.8 <γ <37.5, especially adding a solvent of 20.7 ≤ γ ≤ 24.3, mixing High AC derived from natural products
The component having E inhibitory activity precipitates very easily and in a high yield as a water-soluble precipitate, and this precipitate can obtain the expected effect by adding a small amount to various foods and drinks as an active ingredient. It does not impair the physical properties and functionalities of the added foods and drinks, and is also excellent in heat stability. By blending this water-soluble precipitate as an active ingredient, hypertension has an excellent ACE inhibitory effect. The present inventors have found that a food and drink for preventing hypertension that is effective for prevention can be obtained, and have accomplished the present invention.

Accordingly, the present invention relates to the case where a solvent having a relative dielectric constant γ at 20 ° C. of 17.8 <γ <37.5 is added and mixed to one or more species selected from plants belonging to the Solanaceae family, Myrtaceae family, Urushi family and Papaya family. A food or beverage for preventing hypertension characterized by comprising a water-soluble precipitate that precipitates as an active ingredient, and one or two species selected from plants belonging to the Solanaceae family, Myrtaceae family, Urushi family and Papaya family A solvent having a relative dielectric constant γ of 17.8 <γ <37.5 at 20 ° C. is added and mixed as described above, and a water-soluble precipitate that precipitates is collected, and the water-soluble precipitate is added to a food or drink material as an active ingredient. A method for producing a food or drink for preventing hypertension characterized by comprising:

 Hereinafter, the present invention will be described in more detail.

The starting plant used to obtain the active ingredient according to the present invention is one or more selected from plants belonging to the Solanaceae family, Myrtaceae family, Urushi family, and Papaya family.

Raw materials may be used alone or in combination of two or more. Among them, tomato, papaya, guava,
Mango fruit can be used more preferably.

In addition, it is preferable that the raw material plant is preliminarily processed into a shape such as a squeezed product of fresh fruit juice, a concentrate such as puree and paste, a dried product, a powder or the like so as to be able to efficiently perform the processing described later, and is used as a raw material.

In the present invention, the raw material plant is treated with a solvent having a specific dielectric constant.

Here, the relative dielectric constant γ at 20 ° C. is 17.8 as a solvent.
<Γ <37.5, preferably 20.7 ≦ γ ≦ 32.6, more preferably 20.7 ≦ γ ≦ 24.3, and a solvent having a relative dielectric constant outside the above range efficiently separates the ACE inhibitory active component from the raw material plant. Can not do. The solvent is not particularly limited as long as the relative dielectric constant is within the above range, and an organic solvent such as an alcohol-based organic solvent and a ketone-based organic solvent may be used alone or in combination of two or more. A mixed solvent of an organic solvent and water may be used. Among them, acetone, methanol, ethanol and the like are preferable. Further, the amount of the solvent used is not particularly limited, but it is 50 to 300 with respect to the solid content of the raw material plant. % (% By weight, hereinafter the same), particularly preferably in the range of 100 to 200%. When the amount of the solvent used is less than 50%, some insolubles are precipitated, but it is difficult to obtain a precipitate, and the yield of the precipitate may be extremely deteriorated. It is difficult to increase the yield to the extent that can be expected, and in any case it can be industrially disadvantageous. In this case, the treatment with the solvent is performed by adding a considerable amount of the solvent to the raw material plants, stirring, shaking, etc., and then allowing the mixture to stand for a while, as in the case of the usual extraction method conventionally performed. It can be carried out. The treatment conditions are not particularly limited, but the mixture is usually left at a temperature of 15 to 30 ° C. for 2 to 3 hours.

As a result of the above treatment, a water-soluble precipitate in the raw material plant precipitates and precipitates in an oily or powdery state. This precipitate can be separated and collected by a usual method such as filtration, centrifugation, decantation, etc., and the obtained precipitate has a very high specific ACE inhibitory activity as compared with the raw plant before the above treatment. However, a sufficient effect can be expected with a small intake. Furthermore, the ACE inhibitory activity of this precipitate is stable to heat. Further, the molecular weight of the ACE inhibitory active substance in this precipitate is larger than 60 and about 10,000 or less, and does not develop color with ninhydrin.

The food and drink for preventing hypertension of the present invention is obtained by blending a water-soluble precipitate obtained by treating a raw material plant with a specific solvent as an active ingredient as described above. The precipitate layer containing may be blended as it is after being separated and collected, or may be used after being concentrated or evaporated / dried, and then redissolved in water.

The amount of the water-soluble precipitate is 0.01 to 0.01% of the entire food or drink.
A range of 50%, especially 1-5% is desirable. 001% blended amount
If it is less than 50%, the effect of preventing hypertension may not be expected. If it exceeds 50%, it may be disadvantageous in terms of cost and may be inappropriate as ordinary food.

The food and drink for preventing hypertension of the present invention can be prepared in various forms, such as drinks, dressings, sauces, healthy vinegar, soups, sweets such as candies and gums, and the above-mentioned active ingredients depending on the form. In addition, optional components can be blended. Examples of the optional components include sugars, alcohols, flavors, vitamins and the like in the case of drinks, and vinegar, edible oil, spices and seasonings in the case of dressings.

Effect of the Invention Since the food for preventing hypertension of the present invention contains a component having a high ACE inhibitory activity derived from natural products as an active ingredient, it is widely used as a functional food in the form of drinks, various seasonings, confectionery and the like. It can be used and is extremely useful for preventing hypertension.

 Next, an experimental example will be described.

[Experimental Example 1] To 500 g of tomato paste (approximately 6-fold concentrated), double the amount of each of the various organic solvents shown in Table 1 was added, and after removing the fiber content, the precipitated precipitate was measured for ACE inhibitory activity according to the following method. .

 The results are shown in Table 1.

The ACE inhibitory activity was measured at a 20-fold dilution.

The measurement method was HSCheung and DWCushman: Biochim.Bio
phys. Acta. 293,451 (1973), and using enzymatic reaction with hypopril pistidyl leucine as substrate
Used was derived from rabbit. Add sample and at 37 ° C 30
After reacting for 1 minute, the generated pistydylleucine was replaced with o-
Fluorescence was emitted with phthaldialdehyde and the fluorescence intensity was measured. ACE of the sample with the fluorescence intensity without sample added as blank
The inhibition rate (%) was determined.

From the results in Table 1, the ACE inhibitor in tomato was at 20 ° C
It was found that by treating with a solvent having a relative dielectric constant γ of 17.8 <γ <37.5 to obtain a precipitate, it was possible to efficiently and simply concentrate a large amount.

[Experimental Example 2] 200 ml of acetone was added to 100 g of each paste,
ACE inhibition activity of the deposited precipitate was measured (measurement was 2
The dilution was performed by a factor of 0). The results are shown in Table 2.

[Experimental example 3] To 500 g of tomato paste (approximately 6-fold concentrated), twice the amount of acetone was added, and after removing the fiber content, a deposited precipitate (hereinafter, referred to as acetone precipitate) was obtained.

Appropriately remove tomato paste and acetone precipitate (inhibition rate 0
And ACE inhibition was measured. The value was converted per 1 ml to derive the specific inhibitory activity of the acetone precipitate on the tomato paste.

 The results are shown in FIG.

From the results shown in FIG. 1, it was confirmed that the acetone precipitate had an ACE inhibition rate of about 4.6 times as high as that of the tomato paste, and that a component having a high ACE inhibition activity could be separated by treating with acetone.

[Experimental Example 4] The acetone precipitate of tomato obtained in the same manner as in Experimental Example 2 was diluted with water, heated in a water bath at 100 ° C for 1 hour, and the ACE inhibitory activities before and after heating were compared.

 The results are shown in FIG.

From the results shown in FIG. 2, it was confirmed that the component having ACE inhibitory activity in the acetone precipitate was stable at 100 ° C. for 1 hour.

Hereinafter, the present invention will be described specifically with reference to examples.
The present invention is not limited to the following examples.

Example 1 To 500 g of tomato paste was added 500 ml of ethanol, and the mixture was shaken well and left at room temperature for 3 hours. After standing, the supernatant was removed by centrifugation, and 10 ml of water was added to the obtained precipitate to obtain an active ingredient liquid 1.

Using the obtained active ingredient liquid 1, a drink having the following formulation was prepared.

Drink Active ingredient liquid 1 10 ml Liquid sugar 80 Honey 10 Wine 10 Flavor 2 Vitamin C 2 water 886 total 1000 ml [Example 2] Using the active ingredient 1 obtained in Example 1, a dressing having the following formulation was prepared.

Dressing Vinegar 50 ml Salad oil 140 Salt 5 Spices and seasonings 0.5 Active ingredient liquid 1 4.5 Total 200 ml [Example 3] Healthy vinegar Tomato vinegar 90 ml Active ingredient liquid 1 10 Total 100 ml [Example 4] Confectionery (candy) sugar 35 g syrup 60 active ingredient liquid 1 4.4 perfume 0.6 total 100 g [Example 5] To 500 g of tomato paste was added 750 ml of acetone, and the mixture was shaken well and allowed to stand at 20 ° C for 2 hours. After standing, the resulting precipitate was centrifuged and evaporated to dryness.
Further, it was dissolved in 10 ml of water to obtain an active ingredient liquid 2.

Using the obtained active ingredient liquid 2, a soup having the following formulation was prepared.

Soup Tomato puree 33 g Sugar 6 Flour 2 Salt 2 Onion powder 0.5 Spice 0.01 Active ingredient liquid 2 1 Water Balance 140 g [Example 6] Gum Gum base 36 g Sugar 45 Glucose 10 Corn syrup 5 Active ingredient liquid 2 2 Flavor 2 Total 2 100g

[Brief description of the drawings]

FIG. 1 is a graph showing the specific ACE inhibitory activity of the precipitate obtained by the acetone treatment according to the present invention with respect to the starting plant, and FIG. 2 is a graph showing the thermal stability of the precipitate obtained by the acetone treatment.

Claims (2)

(57) [Claims] (1) at least one kind selected from plants belonging to the Solanaceae family, Myrtaceae family, Urushi family and Papaya family at 20 ° C.
A food or beverage for preventing hypertension, characterized by comprising, as an active ingredient, a water-soluble precipitate that precipitates when a solvent having a relative dielectric constant γ of 17.8 <γ <37.5 is added and mixed. 2. A method according to claim 1, wherein said plant is selected from the group consisting of plants belonging to the family Solanaceae, Myrtaceae, Urushiaceae and Papaya family at 20 ° C.
A solvent having a relative dielectric constant γ of 17.8 <γ <37.5 is added and mixed, and a water-soluble precipitate that precipitates is collected, and this water-soluble precipitate is blended as an active ingredient in a food or drink material. For producing a food or drink for preventing hypertension.