JP2696701B2 - Grafting method - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a grafting method for grafting using a cultured tissue of a plant.

[Conventional technology]

In the conventional grafting method, a seedling germinated from seeds is used as a graft or a branch is cut like a tree and used as a graft.

These methods prevent gene disruption due to breeding,
It is also used to change the characteristics (such as disease resistance) of the root and above-ground parts.

Recently, the 10th Society of Plant Tissue Culture “Proliferation of Acute Wilt-resistant Lines of Hydrangea for Watermelon Rootstock by Tissue Culture”
As in (Taisei Kan et al.), F2 and F3 are selected, and a homogenous clone is obtained by culture and propagation, and used for grafting.

However, in these methods, after the plant has grown to a large size of several tens of millimeters, grafting is performed manually.

Therefore, research on grafting robots has been started to mechanize this manual work, but this is a method of replacing the work that had been manually performed in the past with a machine as it is.

[Problems to be solved by the invention]

The above-described conventional grafting method requires craftsmanship in cutting and grafting rootstocks, and thus is not suitable for mass production.

Currently, robotization is mainly studied by controlling the robot by recognizing the shape and degree of growth in a pattern.However, for the grafted part, the object to be recognized is large and the efficient automatic grafting method is used. It seems difficult to develop.

[Object of the invention]

The present invention has been made in view of the above circumstances, and its purpose is to use a cultured plant tissue as a contact material in a large amount, automatically and efficiently, and to reverse the buds. It is an object of the present invention to provide a grafting method that can be grafted without order.

〔Example〕

 Hereinafter, embodiments of the present invention will be described with reference to the drawings.

FIG. 1 shows a cutting device for automatically and sequentially cutting a cultured plant tissue serving as a contact material into a bud portion and a root portion.

This cutting device includes a culture tank 6 into which an air supply pipe 7 is inserted. Numeral 8 denotes a liquid feed pipe, and the base of the liquid feed pipe 8 is inserted into the culture tank 6.
The pressure feed pump 9 is arranged in the middle part of.

The liquid feeding pipe 8 is provided with a posture aligning device A including a first detecting unit 11, an inverting unit 12, and a second detecting unit 13 from the pressure feeding pump 9 toward the pipe opening 10. And the first of this
Is composed of a combination of a light-emitting device 14 and a light-receiving device 15, and a liquid-feeding tube 8 is narrow between the light-emitting device 14 and the light-receiving device 15, and can be either an embryoid body or a shoot (bud). The cultured plant tissue can pass through the liquid feeding pipe 8 only in the longitudinal direction.

The reversing section 12 is incorporated in the liquid feed pipe 8 as shown in FIG. 2, rotatably accommodates a rotor 18 having a passage 17 in a case 16, and inverts the rotor 18 by a motor or the like. It is like that.

The second detector 13 includes a light emitting device 19 and a light receiving device 20.

A cutter 22 operated by a working cylinder 21 is provided below the mouth 10 of the liquid sending pipe 8.

Therefore, gas is sent from the air supply pipe 7 into the culture tank 6, and the plant tissue 23 cultured in the culture tank 6 passes through the liquid supply pipe 8 by the pump for pumping 9, and the first of the posture-aligning device A To the detection unit 11. Since the liquid supply pipe 8 in the first detection unit 11 is narrow as described above, the plant tissue 23 can pass only in the longitudinal direction, and the shape of the plant tissue 23 is identified by the first detection unit 11. Is done. When the bud of the plant tissue 23 comes first, when the plant tissue 23 enters the passage 17 of the rotor 18 of the reversing unit 12, the rotor 18 is reversed, and the The plant element 23 is inverted. Next, the second detecting unit 13 instructs the plant tissue 23 to be correctly positioned in the mouth 10, and pumps or stops the liquid by the pump 9 for pumping to set the plant tissue 23 in the mouth 10. I do.

Next, as shown in FIG. 3, the working cylinder 21 is operated to cut off the root 24 from the embryoid body, which is the plant tissue 23, with a cutter 22 to obtain a contact material.

It should be noted that the plant tissue 23 entering the liquid sending pipe 8 is sieved to select a tuned product, or to tune it in the culture tank 6 for use. A bypass may be provided to the culture tank 6. In this way, the plant tissue 23 with uniform growth is correctly set in the mouth 10.

Also, the first and second detection units 11 and 13 of the posture alignment device A
Was configured with an optical system of the light emitters 14, 19 and the light receivers 15, 20,
The detection unit may use an electromagnetic field or the like.

The plant tissue obtained by cutting the root 24 thus obtained
The grafted material using 23 is grafted to the root of another plant tissue prepared by cutting out the buds in advance. In this case, the plant tissue serving as the root is a rootstock from which the bud of the plug seedling that has been tissue-cultured or aseptically cultured has been removed, or a tissue that has been tissue-cultured in a culture tank, and from which the bud has been removed by the cutting device described above. The root is used.

In the case where a plant tissue obtained by tissue culture in a culture tank is used as the above-described plant tissue as a root, cutting of the root from the plant tissue is performed using the cutting device used for obtaining the above-mentioned adhesive material. In this case, FIGS. 4 (a), (b), (c),
As shown in (d), the root 24 ′ of the plant tissue 23 ′ used for the root set at the mouth 10 of the liquid sending pipe 8 of the cutting device is replaced with a gel 25 such as alginic acid or polyvinyl alcohol, a sponge or a soft gel. It is stabbed into tissue, rubber, etc., and cut with a cutter 22. As a method of burying the root 24 ′ in the gel 25, a container may be set below the root 24 ′, and the raw material of the gel 25 may be put into the container to gel, and the root 24 ′ may be wrapped by the gel 25.

A planting material 23 serving as a contact material obtained by cutting with another cutting device is placed on the root portion 24 'thus obtained, and grafting is performed. At this time, if necessary, the plant tissue 23 serving as a contact material
Is also wrapped in gel 27 (FIGS. 4 (f), (g), (h)
reference).

In addition, when the plant tissue to be used is in a heterotrophic state, the gels 25 and 27 contain various elements and sugars so that they can grow after grafting, and a drug for preventing various bacteria, an antibiotic,
It is preferable to include a water evaporation inhibitor, a growth regulator (plant hormone), and the like. This creates the root 24 'and
The survival rate of the root portion 24 'and the plant tissue 23 serving as a contact material is improved.

In this way, embryoid bodies, adventitious embryos, adventitious buds and the like are grafted as tissues whose top and bottom are different.

〔The invention's effect〕

As described in detail above, in the grafting method according to the present invention, individual plant tissues obtained by culturing plants in a culture solution are taken out of the culture solution one by one by suction means, and the posture is taken. After the roots were aligned to the leading position by the aligning device, the roots were cut off, and then the buds were grafted to the roots of other plant tissues. Each piece is sent to the cutting device in order and cut and grafted sequentially, whereby a large number of plant tissues cultured in the culture tank are automatically and efficiently inverted, and the buds and roots are inverted. It can be grafted orderly without becoming.

[Brief description of the drawings]

FIG. 1 is an explanatory view of a configuration of a plant tissue cutting device, FIG. 2 is a cross-sectional view of a reversing part, FIG.
(A)-(h) is explanatory drawing which shows a mode that a root part is cut | disconnected and a bud part is grafted to a root part. 6 is a culture tank, 7 is an air feeding pipe, 8 is a liquid feeding pipe, 9 is a pressure feeding pump, 10 is a port, 11, 13 is a detecting section, 12 is a reversing section, 22 is a cutter, 23, 23 'is plant tissue, 24 and 24 'are roots, 25 and 27 are gels, and A is a posture alignment device.

Claims (1)

(57) [Claims] An individual plant tissue obtained by culturing a plant body in a culture solution is taken out of the culture solution one by one by suction means, and is brought into a posture in which a root is advanced by a posture alignment device. A grafting method characterized by cutting the root portion after aligning, and then grafting the bud portion to the root portion of another plant tissue.