JP2584693B2 - Liquid separation device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid separation device,
In particular, the present invention relates to a liquid separation device for separating plasma, red blood cells, and the like from a flexible container such as a blood bag.

[0002]

2. Description of the Related Art In the past, the harmful effects of whole blood transfusion during blood transfusion have been regarded as a problem. Currently, by transfusing only the components necessary for the patient among the components of the blood, the physical burden and immunity of the patient are increased. Blood transfusion is generally performed for the purpose of minimizing side effects caused by such factors. Therefore, it is necessary to prepare a component preparation, and in order to prepare a component preparation, it is necessary to fractionate the blood in the blood bag into each component by a centrifugal separator and separate each component into a separation bag.

An apparatus for separating the blood in the blood bag into respective components is described in Japanese Patent Application Laid-Open No. 63-119776.

That is, the conventional liquid separating apparatus comprises a plurality of container holders for holding a parent bag and a plurality of child bags connected by tubes, and a plurality of tubes for clamping tubes communicating with each bag. A clamper, a container pressurizing unit that pressurizes the parent bag held in the container holding unit and partially extrudes and separates the liquid in the parent bag, a spring balance provided in the container holding unit for each child bag, and the like. A weight measuring unit, and a control unit that controls the tube clamper and the container pressurizing unit, and enables the liquid separated from the parent bag to be transferred to the communicating child bag via the unclamped tube. It is configured.

[0005] In the first separation operation, the control section pushes the plasma layer in the parent bag into the child bag by the pressurizing section. In the second separation operation, the platelets in the child bag are pushed out to another child bag by the pressurizing unit.

At this time, in the prior art, the container pressurizing portion is
A moving element driven by a pressure driving source, a pressing plate capable of pressing against the container, and an urging device interposed between the moving element and the pressing plate to apply a pressing force to the pressing plate. The biasing device is constituted by a single spring body.

[0007]

However, in the prior art, the urging device of the container pressurizing portion is constituted by a single spring, and the container pressurizing portion is connected to the parent bag by the first separating operation. The applied pressure is equal to the applied pressure on the child bag in the second separation operation. For this reason, the pressing force is too small for the parent bag with a large bag size, and too large for the child bag with a small bag size, and the separation operation in each bag cannot always be performed smoothly.

Particularly, in the prior art, the interface of the liquid fractionated in the container being pressurized by the container pressurizing unit is detected by the interface detecting unit, and the detection interface of the interface detecting unit is preset. In the liquid separating apparatus capable of recognizing that the control unit has reached the interface setting position, if the pressure of the container pressing unit is adapted to the first separating operation of pressing the parent bag described above, In the second separation operation in which the bag is pressurized, the extrusion flow rate is too high, and the interface is disturbed, and there is a possibility that the interface cannot be detected.

According to the present invention, when a container is pressurized to partially extrude and separate a liquid in the container, a pressing force suitable for the container to be separated is applied, and the separating operation in the container is performed smoothly. With the goal.

According to the present invention, when detecting the interface of a liquid to be extruded and separated in a container and controlling the amount of liquid separation, a pressing force suitable for the container to be separated is applied, and the extrusion flow rate in the container is adjusted. The purpose of the present invention is to make the separation operation in the vessel smooth without causing the interface to be disordered by optimizing the separation.

Means for Solving the Problems According to the first aspect of the present invention, a flexible container accommodating a layered liquid is pressurized and a part of the liquid in the container is pressurized. In a liquid separation device configured to have a container pressurizing unit that extrudes and separates, the container pressurizing unit includes a moving element driven by a pressure driving source, a pressing plate capable of pressing against the container, and a moving element. And a biasing device interposed between the pressure plate and the pressing plate to apply a pressing force to the pressing plate. The biasing device can add a plurality of spring bodies having different spring constants and displace each spring body. The magnitude of the pressing force exerted on the pressing plate by the spring body can be changed.

Further, according to the present invention as set forth in claim 1, the plurality of spring bodies are connected in series in a pressing direction, and each time the moving element exceeds a predetermined moving distance from the original position. The spring body having a larger spring constant can be displaced, and a larger pressing force based on the displacement of the spring body can be applied to the pressure plate.

According to the present invention, there is further provided the present invention as set forth in claim 1, wherein the urging device has a large spring constant supported by one of the moving member-side receiving member and the pressure plate-side receiving member. A second spring body supported by the other spring body and having a small spring constant; and a second spring body displacement preventing means interposed between the two spring bodies. Body displacement prevention means, when the mover exceeds a certain amount of movement from the original position,
The second spring body abuts against the moving element-side receiving member or the pressure plate-side receiving member to prevent the displacement of the second spring body. The displacement of the body can be started.

According to a second aspect of the present invention, there is provided a container holding portion for holding a container, containing a liquid separated in layers, and pressurizing a flexible container held in the container holding portion. A container pressurizing unit for partially extruding and separating the liquid in the container, an interface detecting unit for detecting an interface of the fractionated liquid in the container, and an interface setting for setting an interface setting position for the container And a control unit that can recognize that the detection interface of the interface detection unit has reached the interface setting position based on the setting result of the interface setting unit and the detection result of the interface detection unit. In the separation device, the container pressurizing unit includes a moving element driven by a pressure driving source, a pressing plate capable of being pressed against the container, and a pressing force interposed between the moving element and the pressing plate to apply pressure to the pressing plate. And a plurality of biasing devices having different spring constants. A spring member, and it can be displaced each spring body, in which the spring body is configured to allow changing the size of the pressure on the pressure plate.

Further, according to the present invention as set forth in claim 2, the plurality of spring bodies are connected in series in the pressing direction, and each time the moving element exceeds a predetermined moving distance from the original position. The spring body having a larger spring constant can be displaced, and a larger pressing force based on the displacement of the spring body can be applied to the pressure plate.

According to a second aspect of the present invention, in the second aspect of the present invention, the urging device has a large spring constant supported by one of the moving element-side receiving member and the pressure plate-side receiving member. 1
A spring body, a second spring body having a small spring constant supported by the other of the two receiving members, and a second spring body displacement preventing means interposed between the two spring bodies. The second spring body displacement prevention means abuts against the moving element-side receiving member or the pressure plate-side receiving member supporting the second spring body when the moving element exceeds a predetermined amount of movement from the original position. The displacement of the second spring body is prevented, and when the moving element exceeds the moving amount, the first
The displacement of the spring body can be started.

[0017]

According to the first aspect of the present invention, the following operations are provided.

The container pressurizing section can apply a pressing force corresponding to the spring constant of the spring to the pressurizing plate by displacing the spring body. Therefore, when the container is pressurized to partially extrude and separate the liquid in the container, by displacing the spring body, a pressing force suitable for the container to be subjected to the separation operation is applied, and the separation operation in the container is performed. It can be done smoothly.

Further, according to the present invention, the following operations are provided.

The container pressurizing section displaces a spring body having a larger spring constant every time the moving element exceeds a certain amount of movement, and applies a larger pressing force based on the displacement of the spring body to the pressurizing plate. be able to. Therefore, when the container is pressurized to partially extrude and separate the liquid in the container, by adjusting the amount of movement of the mover, a pressing force suitable for the container to be subjected to the separation operation is applied, and The separation operation can be performed smoothly.

Further, according to the present invention, the following operations are provided.

The container pressurizing section applies a small pressing force to the pressurizing plate based on the displacement of the spring body having a small spring constant when the movable element is within a certain small moving range on the original position side. When the movable element is in the large movement range beyond the small movement range, a large pressing force based on the displacement of the spring body having a large spring constant is applied to the pressure plate. This makes it possible to reliably select and apply two levels of pressures, large and small, to the container to be subjected to the separation operation, and it is possible to smoothly perform the separation operation in the container.

According to the second aspect of the present invention, the following operations are provided.

In controlling the liquid separation amount by detecting the interface of the liquid to be extruded and separated in the container, by applying the same effect as described above, a pressing force suitable for the container to be separated is applied. Then, the separation operation in the container can be smoothly performed without causing disturbance of the interface by optimizing the extrusion flow rate in the container.

Further, according to the present invention, the following operations are provided.

In controlling the liquid separation amount by detecting the interface of the liquid to be extruded and separated in the container, by applying the same effect as described above, a pressing force suitable for the container to be separated is applied. Then, the separation operation in the container can be smoothly performed without causing disturbance of the interface by optimizing the extrusion flow rate in the container.

Further, according to the present invention, the following operations are provided.

In controlling the amount of liquid separation by detecting the interface of the liquid to be extruded and separated in the container, by applying the same effect as described above, a pressing force suitable for the container to be separated is applied. Then, the separation operation in the container can be smoothly performed without causing disturbance of the interface by optimizing the extrusion flow rate in the container.

[0029]

FIG. 1 is a perspective view showing an example of a liquid separating apparatus, FIG. 2 is a block diagram showing a control system of the liquid separating apparatus, FIG. 3 is a sectional view showing a main part of FIG. 1, and FIG. FIG. 4A is an arrow view along line AA, FIG. 4B is an arrow view along line BB in FIG. 4A, FIG. FIG. 7 is a sectional view showing a main part of FIG. 1, FIG. 7 is a sectional view taken along the line VII-VII of FIG. 6, FIG. 8 is a flow chart showing a first separation operation, FIG.
Is a flowchart showing a second separation operation.

The liquid separation device 20 is mainly used for separating components of blood.
0 ml and 400 ml, respectively, double bag, triple bag and quadruple bag for leukocyte removal (with or without Additive Solution)
Can be used. In this example, 400 ml
4 bags for leukocyte removal are used.

The automatic liquid separating apparatus 20 comprises a main container holding portion 21 for holding a main bag 2a provided with a liquid take-out tube 11, and a main bag 2a held by the main container holding portion 21.
And a container pressurizing unit 22 that pressurizes blood components in an upper layer in the parent bag 2a, and an upper portion that is extruded by the container pressurizing unit 22 from the liquid extraction tube 11 of the parent bag 2a via each tube. First and second containing blood components of layers
And first to third holding the third child bags 2b, 2c and 2d.
Third sub-container holding units 23A to 23C, opening / closing unit 24 for opening / closing each tube, interface detecting unit 25 for detecting an interface of blood in blood bag 2, and each child bag 2b, 2c, 2d
Measuring unit 10 for measuring the weight of blood components contained in
1, 102, the detection signal detected by the interface detection unit 25, the measurement signal measured by each of the weight measurement units 101 and 102, and the container pressurizing unit 22 and the opening / closing unit 24 based on a program input in advance. And an arithmetic control unit 26 that arithmetically outputs an operation signal for operating the control unit.

The main container holder 21 is provided with the operation box 2
3 and can be opened and closed as indicated by the arrow in FIG.
7 and the main container holding part 21 is closed, as shown in FIG.

The container pressurizing section 22 includes an operation box 27.
The pressing plate 10 fixed to the rotating shaft 8 supported by the pressure plate 10 is pressed toward the container holding portion 21 by a pressing mechanism.
Between the parent bag 2a and the child bag 2
b (see FIG. 5).

The pressing mechanism of the container pressing section 22 is shown in FIGS.
As shown in (A) and (B), a moving member 113 which is screwed to a feed screw 112 driven by a pressing motor 111 and moves in the direction of the feed screw 112;
And an urging device 114 interposed between the pressure plate 10 and the pressure plate 10 to apply a pressing force to the pressure plate 10.

In this case, the urging device 114 is provided as shown in FIG.
As shown in (B), the cylindrical member 115 and the mandrel 11
6, a first spring body 117 and a second spring body 118. The tubular member 115 is loosely inserted into the movable member 113 and includes both ends spring receiving portions 115A and 115B. Mandrel 1
Reference numeral 16 denotes a pressing plate side receiving member 116B which is inserted into the cylindrical member 115, includes a spring receiving portion 116A at one end inside the cylindrical member 115, and is pin-connected to the pressing plate 10.

The first spring body 117 and the second spring body 118
The first spring body 117 has a larger spring constant, the first spring body 117 is provided around the cylindrical member 115, and the second spring body 118 is provided inside the cylindrical member 115. The first spring body 117 and the second spring body 118 are connected to the first spring body 11.
7 and the spring receiving portion 115A, and the second spring body 118 and the spring receiving portion 115B are connected in series in the pressing direction, and the first spring body 117 is connected to the mover-side washer. 113A
The second spring body 118 is supported by the mandrel 116.
The front surface is supported by the spring receiving portion 116A.

At this time, the tubular member 115 is
A mandrel 116 supporting the second spring body 118 when the movable element 113 exceeds a certain small movement amount from the original position by being interposed between the first and second spring bodies 118 and 118. Against the pressure plate side receiving member 116B of the
The displacement of the spring body 118 is prevented, and when the movable element 113 moves by a large amount of movement exceeding the small amount of movement, the first spring body 117 is moved.
Is started to be displaced. A detection dog 119 is fixed to the lower surface of the movable element 113, and an original position detection switch 119A, a small movement amount detection switch 119B, and a large movement amount detection switch 119C for detecting the detection dog 119 are provided on the movement path of the movable element 113. is set up.

That is, the container pressurizing section 22 has the following (A):
It operates as shown in FIG.

(A) Small pressure application stage

When the arithmetic and control unit 26 causes the pressurizing circuit 30 to rotate the motor 111 in the normal direction, the feed screw 112 rotates, the moving element 113 starts moving from the original position, and the second spring body having a small spring constant. 118 is the spring receiving portion 1 of the cylindrical member 115.
15B and the spring receiving portion 116A of the mandrel 116 are compressed and displaced.

The moving element 113 stops at a position where the moving amount exceeds the small moving amount detected by the small moving amount detecting switch 119B.

Thus, the compressed second spring body 11
The small pressing force of 8 gradually pushes the pressing plate 10, and the pressing plate 10 presses the child bag 2 b with a small pressing force between the pressing plate 10 and the container holding unit 21.

(B) High pressure application stage

When the arithmetic and control unit 26 causes the pressurizing circuit 30 to rotate the motor 111 in the normal direction, the feed screw 112 rotates and the mover 113 starts moving from the original position, and the second spring body having a small spring constant. 118 is the spring receiving portion 1 of the cylindrical member 115.
15B and the spring receiving portion 116A of the mandrel 116 are compressed and displaced.

When the moving element 113 is the small movement amount detection switch 1
When the small movement amount exceeds the small movement amount corresponding to 19, the cylindrical member 115 as the second spring body displacement prevention means has the pressing plate-side receiving member 116 of the mandrel 116 supporting the second spring body 118.
B, and prevents the displacement of the second spring body 118. At this time, the motor 111 continues to rotate forward, the moving element 113 further moves, and the first spring body 117 having a large spring constant is moved to the moving element side receiving washer 113A and the spring receiving portion 115A of the cylindrical member 115.
And compression displacement.

The moving element 113 stops at a position where the moving amount exceeds the large moving amount detected by the large moving amount detecting switch 119C.

Thus, the compressed first spring body 11
The pressing force of 7 gradually pushes the pressing plate 10, and the pressing plate 10 presses the parent bag 2 a with a large pressing force between the pressing plate 10 and the container holding unit 21.

That is, at the time of the first separation operation in which the parent bag 2a is to be pressed, the arithmetic control unit 26 sets the motor 1 in the above (B).
At the time of the second separation operation for controlling the drive of the motor back 11 and pressing the child back 2b, the arithmetic and control unit 26 sets the motor 1 in the above (A).
11 is driven and controlled.

The liquid separating device 20 includes an operation box 2
7. A lid 32 attached to the upper surface 27b of the printer 7 so as to open and close.
have. The lid 32 can be vertically fixed to the upper surface 27b. The lid 32 has a hook 33 so that two child bags can be suspended.
a and 33b are provided, and a tray 35 on which one child bag can be placed is provided on the upper surface 26b of the operation box 27.

The opening / closing section 24 includes first, second, third and fourth tube clampers 37, 38 and 39 provided on the upper surface 26b of the operation box 27, respectively. 38 and 39 are opened and closed by solenoids 36a, 37a, 38a and 39a, respectively.

Each of the solenoids 36a, 37a, 38a
And 39a are driving circuits 36b, 37b, 38b, and 3
9b and the input / output port 31 are electrically connected to the arithmetic and control unit 26, respectively.

The interface detecting section 25 provides an interface between the plasma layer C and the buffy coat layer D of the blood bag 2 after centrifugation, and / or
Alternatively, an interface between the buffy coat layer D and the red blood cells E is detected, and a photosensor is generally used, and the interface is detected based on a difference in the light absorptivity of each layer. The mounting position of the interface detecting section 25 can be slightly corrected up and down. The interface detecting unit 25 is electrically connected to the arithmetic and control unit 26 via the input / output port 31.

The interface detecting section 25 is specifically configured, for example, as shown in FIG. That is, in FIG.
Reference numeral 21 denotes a light emitting device provided on the pressing plate 10, and 122 denotes a light receiving device provided on the side surface 27 a of the operation box 27. The first to first light emitting devices 121 extend along the up-down direction of the blood bag 2 set in the main container holding portion 21.
5, a light receiving device 1 comprising 15 infrared light emitting diodes.
Reference numeral 22 denotes two plate-like light emitting diodes connected in parallel to all the light emitting diodes of the light emitting device 121.

At this time, the liquid separation device 20 should set the interface setting position (the position of the interface that should be at the end of the separation) with respect to the blood bag 2 set in the main container holder 21 in the blood bag 2 at the end of the current separation. An interface setting unit 50 is provided for setting according to the plasma residual amount (accordingly, according to the volume of the blood bag 2 used this time or the centrifugation conditions in the previous step). In addition, the liquid separation device 20 stores the interface setting position set by the interface setting unit 50 in a nonvolatile EEPROM 40c of the storage unit 40 described later. The nonvolatile EEPROM 40c can rewrite and recall the above-mentioned stored data, and does not erase the stored data even when the main power is turned off.

However, the method in which the interface detecting section 25 detects the interface position set by the interface setting section 50 can be, for example, as follows.

The arithmetic control unit 26 sets the detection work position of the interface detecting unit 25 to the current parent bag 2a or the child bag 2b.
c, the main light emitting diode (for example, the eighth light emitting diode) located at the detection work position, and the upper light emitting diode (for example, the sixth light emitting diode) located at the upper and lower sides of the detection work position corresponding to the interface setting position ) And a lower light emitting diode (for example, a tenth light emitting diode) are sequentially and repeatedly caused to emit light.

At the same time, the arithmetic and control unit 26 controls the light receiving device 12
The amount of light received by the light-receiving diodes constituting the second light-emitting diode is transferred after A / D conversion, and the difference (S6-S10) between the light-receiving amounts S6 and S10 of the sixth light-emitting diode and the tenth light-emitting diode is calculated to obtain the eighth light emission When the amount of light received by the diode satisfies the following equation, it is recognized that the interface of the blood bag 2 has reached the interface setting position.

[0058]

[Equation 1] S8 ≒ (S6-S10) / 2

The weight measuring units 101 and 102 are electrically connected to the arithmetic and control unit 26 via the weight measuring circuit 35 and the input / output port 31, and are specifically configured as follows.

As shown in FIG. 3, the weight measuring unit 101 for the first child bag 2b provided in the first sub-container holding unit 23A is provided with a bag receiving tray 104 on which the child bag 2b is mounted.
Is supported on the weight sensor 105.

The weight measuring unit 1 for the child bags 2c, 2d provided in the second and third sub-container holding units 23B, 23C.
02 is a child bag 2c, as shown in FIGS.
The hooks 33a and 33b on which the 2d is suspended are supported by a single weight sensor 106. That is, the two container holders 23B and 23C share a single weight measuring unit 102, and selectively measure the weight change of the child bags 2c and 2d held in the respective container holders 23B and 23C. it can.

The weight sensors 105 and 106 are supported by sensor holding brackets 105A and 106A fixed to the operation box 27 and the lid 32, respectively, and are attached to two positions on the upper surface and two positions on the lower surface, respectively. It is configured to include a strain gauge forming a circuit.

At this time, the liquid separating device 20 determines the amount of liquid to be stored in each of the child bags 2b to 2d set in each of the first to third sub-container holding portions 23A to 23C by using the child bag 2b used this time. A liquid amount setting unit 60 for setting according to the capacity of 2d to 2d is provided. The liquid separating device 20 stores the set liquid amount set by the liquid amount setting unit 60 in a nonvolatile EEPROM 40c described later. Storage unit 40
Can rewrite and read the above stored data,
Even when the main power is turned off, stored data is not erased.

Therefore, each of the weight measuring units 101, 102
Measures the liquid volume set by the liquid volume setting section 60 as follows. That is, the arithmetic control unit 26 is configured to execute the nonvolatile EEP
The storage data of the liquid amount setting unit 60 stored in the ROM 40c and the measurement results of the respective weight measuring units 101 and 102 are obtained, and the measurement results of the respective weight measuring units 101 and 102 reach the set liquid amounts. Can be recognized.

At this time, the arithmetic and control unit 26 sets the second and third
The output change amount of the weight measuring unit 102 shared by the sub container holding units 23B and 23C is equal to the container holding units 23B and 23C.
Which one of the child bags 2c and 2d is the one corresponding to the weight change amount is selectively recognized as follows. In other words, the control unit 26 obtains the measurement result of the weight measuring unit 102, and at the same time, the tube 15
The weights of the child bags 2c and 2d communicating with the unclamped tubes 15a and 15b are calculated by recognizing the child bags 2c and 2d communicating with the child bags a and 15b. By recognizing that the child bag 2c or 2d corresponds, the weight change amount of the child bag 2c or 2d can be calculated and output.

The operation control unit 26 includes an input / output port 31
And the storage unit 40 (R
OM 40a, RAM 40b, and EEPROM 40c), and outputs an operation signal to each device via each circuit. The ROM 40a of the storage unit 40 is loaded with a program in which the operation method of the present invention is stored in advance,
It is electrically connected to the arithmetic and control unit 26. This program is, for example, 400 ml, 200 ml, and various other operation methods. By selecting this program with a selection switch, the operation method can be variously changed. In addition, ROM
The program mounted on the 40a may be a system in which a program cassette created for each operation method is exchanged for each operation method.

The ROM (40a) is a "read only memory" and contains a program for automatically performing a liquid separation operation, and its contents may be lost even if the power is turned off. Absent. The contents cannot be rewritten.

The RAM (40b) stores "random
`` Access memory '', which stores the number of the current separation operation in the middle of the liquid separation operation, stores the measurement result of the liquid amount, stores the measurement result of the interface detection unit, This is a memory for storing intermediate results of calculations performed by the calculation control unit. When the power is turned off, all the contents are lost.

The EEPROM (40c) is provided with an "electrically erasable programmable read / write".
It is a "only memory," and the contents can be rewritten by applying a high voltage for writing, even if it is a read-only memory. Also, the stored contents will not be lost even if the power is turned off. In this EEPROM, the interface detection set value and the fluid volume set value for each type of blood bag are all stored, and only when the mode is switched to the setting change mode,
The settings can be rewritten.

That is, the arithmetic control unit 26 is provided with the interface detection unit 25
Of the weight measurement units 101, 10
The liquid separation is automatically performed by operating the container pressurizing unit 22 and the opening / closing unit 24 based on the measurement result measured by the method 2 and the program.

At this time, the arithmetic and control unit 26 sets the data of the interface setting position and the set liquid amount stored in the nonvolatile EEPROM 40C, the detection result of the interface detecting unit 25, and the measurement of each of the weight measuring units 101 and 102. Based on the result, the opening / closing unit 24 is closed by recognizing that the detection interface of the interface detection unit 25 has reached the interface setting position and / or that the measurement results of the weight measuring units 101 and 102 have reached the set liquid amount. Then, the separating operation is completed.

The arithmetic control unit 26 is electrically connected to switches such as a power switch 41, a first start switch 42, a second start switch 43, a stop switch 44, and a liquid crystal display 45 via an input / output port 31. Connected. These switches and the liquid crystal display 45 are attached to a panel portion on the inclined portion 27c of the operation box 27. This liquid crystal display 4
5 is provided on the operation panel of the operation box 27. The name of the program to be used is displayed on the liquid crystal display 45. Therefore, you can check whether this program is correct. In addition, when an error message is present, for example, when there is an abnormality in the interface level or the weighing scale (the child bag is not set at a predetermined position), the fact is displayed on the liquid crystal display 45.

Next, the automatic liquid separating apparatus 2 having the above configuration
The operation of 0 will be described.

FIG. 8 is a flow chart showing the first separation operation in the case where the first centrifugally separated four-cell leukocyte removal bag (with storage solution) is used in the liquid separation device.

First, the power switch 41 of the automatic liquid separating apparatus 20 is turned on to perform initialization (step (1)). Next, a blood bag (quadruple bag for leukocyte removal) 2 obtained by collecting 400 ml of blood from the donor is subjected to the first centrifugal separation, for example, a blood bag that has been subjected to 3000 to 4000 G for 5 minutes is prepared. The centrifuged blood bag 2 is set in the automatic liquid separator 20 as shown in FIG. The parent bag 2 a containing 400 ml of whole blood in the blood bag 2 is hung on the hooks 7 of the main container holding portion 21. Further, the first child bag 2b is placed on the tray 35 of the first sub-container holding section 23A. That is, it is placed on the bag tray 104 of the weight measuring unit 101 in FIG.

Further, the second and third child bags 2c, 2d
Is hung on the hooks 33a and 33b of the weight measuring unit 102, respectively. Further, the tube 12 of the blood bag 2 is set on the first tube clamper 36, the tube 14 is set on the second tube clamper 37, and the tubes 15a and 15b are set on the third and fourth tube clampers 38 and 39, respectively.

After the blood bag 2 is set in the automatic liquid separating apparatus 20, the program for the blood bag 2, that is, the quadruple bag for removing white blood cells is selected, and this program is selected to select the automatic liquid separating apparatus 1. (Step (2)).

Next, the name of the read program is displayed on the liquid crystal display 45, and it is determined whether or not the type of the blood bag 2 matches the set program by looking at this display (step ( 3)). If the answer to the step (3) is negative, the stop switch 44 is turned on to release the entire surface, and the process returns to the step (2), where a correct program is selected and read from the storage unit 40. If the answer to step (3) is affirmative, the procedure proceeds to step (4), where the first start switch 42 is turned on, and the procedure proceeds to step (5).

In step (5), the detection result of the interface detection unit 25 is obtained, and whether the separation interface level is at a predetermined position is determined.
That is, it is determined whether or not the parent bag 2a is set at a predetermined position.

At this time, since it is displayed on the liquid crystal display 45, it is judged by looking at it. If the answer to the step (5) is negative, the process returns to the step (4) and the stop switch 44 is turned on. To release the entire surface, the position of the parent bag 2a is set at a predetermined position, and the first start switch 42 is turned on again. If the answer to step (5) is affirmative,
Proceeding to step (6), in step (6), it is determined whether or not the weight measuring unit 101 indicates a predetermined weight, that is, whether or not the first child bag 2b of a predetermined type is set at a predetermined position. Whether the weight measurement unit 102 indicates a predetermined weight, that is, the second child bag 2c of the predetermined type,
It is determined whether or not the child bag 2d is set at a predetermined position.

Since this is displayed on the liquid crystal display 45, it is determined by looking at this, and if the answer to the step (6) is negative, the process returns to the step (4) again and the stop switch 44
Is turned on to release the entire surface, the first child bag 2b is set at a predetermined position, and the first start switch 42 is turned on again. If the answer to step (6) is affirmative, the process proceeds to step (7), and the first, second, third and fourth tube clampers 36, 37, 38 and 39 are all closed. Parent bag 2
a, the breakable and separable plug body built in the liquid discharge pipe 11 is folded to connect the parent bag 2a and the tube 12, and at the same time, the break built in the liquid discharge pipe 18 of the third child bag 2d is broken. The detachable stopper is folded and the third child bag 2d
And the tube 15b.

Next, proceeding to step (8), the first start switch 42 is turned on and proceeding to step (9), the first and third tube clampers 36 and 38 are opened, and the second and fourth tube clampers 37 and 39 is closed.

Next, proceeding to step (10), the moving member 113 of the container pressurizing section 22 is moved to a position exceeding the large moving amount detected by the large moving amount detecting switch 119C, and the pressing operation by the pressing plate 10 is performed. To start pressurizing the parent bag 2a. The plasma layer C of the parent bag 2a starts to move to the second child bag 2c.

At this time, the container pressurizing portion 22 is
By displacing the first spring body 117 having a large spring constant as in the step of applying a large pressing force, the pressing plate 10 is pushed by the large pressing force of the first spring body 117, The parent bag 2a is clamped by a large pressing force generated between the container 10 and the container holding portion 21.

Next, proceeding to step (11), the plasma layer C
It is determined whether or not the interface between the film and the buffy coat layer D has reached the set position by the interface detection unit 25. If the answer to the step (11) is negative, the process returns to the step (10) and the pressurization of the pressurizing plate 10 is continued to send the plasma layer C to the second child bag 2c.

If the answer to step (11) is affirmative, the process proceeds to step (12), where the first and second tube clampers 3
6 and 37 are opened, the third and fourth tube clampers 38 and 39 are closed, and a part of the plasma layer C, a part of the buffy coat layer D and a part of the red blood cell layer E are transferred to the first child bag 2b. Next, proceeding to step (13), the measurement result of the weight measuring unit 101 is obtained, and it is determined whether or not the weight of the blood that has entered the first child bag 2b has reached 30 g. If the answer to the step (13) is negative, do not proceed and wait until the weight reaches 30 g. If the answer to step (13) is affirmative, step (14)
Then, the pressurizing of the pressurizing plate 10 is stopped and the step (1) is performed.
Proceed to 5).

In step (15), the second and third tube clampers 37 and 38 are opened, and the first and fourth tube clampers 36 and 39 are closed. Therefore, the plasma layer C contained in the second child bag 2c moves to the first child bag 2b by gravity. Proceeding to step (16), the weight measurement unit 10
Based on the measurement result of No. 1, it is determined whether or not the weight of the plasma contained in the first child bag 2b has reached 30 g + 40 g = 70 g. If the answer to step (16) is negative, the process does not proceed and waits until the weight reaches 70 g.

If the answer to the step (16) is affirmative, the process proceeds to a step (17). In the step (17), the first and fourth tube clampers 36 and 39 are opened, and the second and third tube clampers 37 are opened. And 38 are closed. Therefore,
Red blood cell preservation solution (Addtive) in the third child bag 2d
(Solution) moves to the parent bag 2a under natural flow. Proceeding to step (18), the measurement result of the weight measurement unit 101 is obtained, and the red blood cell storage solution (Ad) containing the parent bag 2a is obtained.
It is determined whether or not the weight of the D.Solution has reached 90 g. If the answer to step (18) is negative, do not proceed and wait until the weight reaches 90 g.

If the answer to step (18) is affirmative, the process proceeds to step (19), where the stop switch is turned on to end the operation, and the first centrifuged blood component separation operation ends. Thereafter, the tubes 12 and 15a are sealed with a tube sealer or the like.
a, The second child bag 2c is cut. The cut parent bag 2a and the second child bag 2c are stored at predetermined locations.

FIG. 9 shows the second centrifugal separation after the first separation operation, for example, the above-mentioned first and third child bags 2b and 2d subjected to the second centrifugation 200G for 5 minutes. 5 is a flowchart showing a two-separation operation.

First, the power switch 41 of the automatic liquid separating apparatus 20 is turned on to perform initialization (step (2)
0). Next, the first and third child bags 2b and 2d are set in the automatic liquid separation device 20. The first child bag 2b is hung on the hooks 7, 7 of the main container holder 21. Next, the third child bag is stored in the room 35 of the first sub-container holding unit 23A and is placed on the bag receiving tray 104 of the weight measuring unit 101.

Further, the tube 14 of the blood bag 2 is
The tube 15b is set on the tube clamper 36 and the second tube clamper 37, respectively.

After setting the first and third child bags 2b and 2d in the automatic liquid separating device 20, the blood bags,
That is, the program for the second centrifugally separated child bag is selected, and this program is read out from the storage unit 40 of the automatic liquid separation device 20 (step (21)). Next, since the name of the selected program is displayed on the liquid crystal display 45, it is determined whether or not the program for the second centrifugally separated child bag matches the read program by looking at this display. (Step (22)). If the answer to the step (22) is negative, the stop switch 44 is pressed to release the entire surface, and the process returns to the step (21). Step (2
If the answer to 2) is affirmative, the process proceeds to step (23), the second start switch 43 is turned on, and the process proceeds to step (24).

In step (24), the weight measuring unit 101
Indicates a predetermined weight, that is, whether the third child bag 2d is set at a predetermined position.

Since this is displayed on the liquid crystal display 45, it is determined by looking at this, and if the answer in step (24) is negative, the process returns to step (23) again, and the stop switch 44 is turned on to turn on the entire surface. It is released, the third child bag 2d is set at a predetermined position, and the second start switch 43 is turned on again. If the answer to step (24) is affirmative, the process proceeds to step (25), and the first and second tube clampers 36 and 37 are opened.

Next, proceeding to step (26), the moving member 113 of the container pressurizing section 22 is moved to a position exceeding the small moving amount detected by the small moving amount detecting switch 119b, and the pressing operation by the pressing plate 10 is performed. To activate the first child bag 2b.
Start pressurizing. Liquid take-out tube 11 of first child bag 2b
The platelets in the first child bag 2b start to move to the third child bag 2d, breaking the breakable and separable plug stopper built therein.

At this time, the pressurizing section 22 is
By displacing only the second spring body 118 having a small spring constant as in the small pressing force applying step of
The pressing plate 10 is pushed with a small pressing force of the spring body 118,
A small pressing force generated between the pressure plate 10 and the container holding portion 21 narrows the child bag 2b.

Then, the process proceeds to a step (27), wherein it is determined whether or not the weight of the platelets contained in the third child bag 2d becomes 40 g based on the measurement result of the weight measuring unit 101. If the answer in step (27) is negative, do not proceed and weight is 4
Wait until it reaches 0 g.

If the answer to the step (27) is affirmative, the routine proceeds to a step (28), in which the pressing of the pressure plate 10 is stopped, and the routine proceeds to a step (29). In step (29), the first and second clampers 36 and 37 are closed, and further proceeding to step (30), the stop switch 44 is turned on, the power switch 41 is turned off, and the state becomes END.
The centrifuged blood component separation operation ends. After this,
Tube 14 or 1 with tube sealer (registered trademark) etc.
5b, the first and third child bags 2b and 2b are sealed.
Cut d.

The first child bag 2b is discarded, and the third child bag 2d is stored in a predetermined place.

Next, the operation of the above embodiment will be described.

In controlling the blood separation amount by detecting the interface of the blood to be extruded and separated in the parent bag 2a and the child bag 2b, the container pressurizing unit 22 causes the movable element 13 to move the movable element 13 in a fixed small movement on the original position side. If it is within the range, the second constant having a small spring constant
A small pressing force based on the displacement of the spring body 118 is applied to the pressing plate 10.
Effect. When the movable element 113 is in the large movement range exceeding the small movement range, a large pressing force based on the displacement of the first spring body 117 having a large spring constant is applied to the pressure plate 10. As a result, the parent bag 2 to be separated
a, and a small pressing force for the child bag 2b can be reliably selected and applied in two stages, large and small, and the extrusion flow rate in each of the bags 2a, 2b is optimized. The separation operation in the bags 2a and 2b can be performed smoothly without causing the disturbance of the interface.

In the embodiment of the present invention, the urging device does not always need to arrange a plurality of spring bodies in series.

Further, in the embodiment of the present invention, the biasing device changes the pressing force applied to the pressing plate to three or more stages by using three or more spring bodies having different spring constants in a stepwise manner. It may be.

[0105]

As described above, according to the first to third aspects of the present invention, when the container is pressurized to partially extrude and separate the liquid in the container, it is compatible with the container to be separated. And the separation operation in the container can be performed smoothly.

Further, according to the present invention, when detecting the interface of the liquid to be extruded and separated in the container and controlling the amount of liquid separation, it is suitable for the container to be separated. Without applying pressure to the vessel and optimizing the extrusion flow rate in the vessel without causing interface disturbances.
The separation operation in the container can be performed smoothly.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an example of a liquid separation device.

FIG. 2 is a block diagram showing a control system of the liquid separation device.

FIG. 3 is a sectional view showing a main part of FIG. 1;

4 (A) is an arrow view along line AA in FIG. 3, and FIG. 4 (B) is an arrow view along line BB in FIG. 4 (A).

FIG. 5 is a sectional view showing a container pressurized state.

FIG. 6 is a sectional view showing a main part of FIG. 1;

FIG. 7 is a sectional view taken along the line VII-VII of FIG. 6;

FIG. 8 is a flowchart showing a first separation operation.

FIG. 9 is a flowchart showing a second separation operation.

[Explanation of symbols]

 2, 2a, 2b, 2c, 2d Bag 12, 14, 15 Tube 20 Liquid separator 21, 23 Container holding unit 22 Container pressurizing unit 24 Opening / closing unit 25 Interface detecting unit 26 Control unit 40 Storage unit 50 Interface setting unit 111 Addition Pressure motor 113 mover 114 urging device 115 cylindrical member 116 mandrel 117 first spring body 118 second spring body

Claims (2)

(57) [Claims] 1. A container pressurizing section (22) for pressurizing a flexible container (2) containing a layered liquid and extruding and separating a part of the liquid in the container (2). In the liquid separation device (20) configured to include: a container pressurizing unit (22) presses the moving element (113) driven by the pressurizing drive source (111) and the container (2). The pressure plate (10) to be obtained and a biasing device (114) interposed between the moving element (113) and the pressure plate (10) to exert a pressing force on the pressure plate (10). , Biasing device (11
4) is a plurality of spring bodies (117, 11) having different spring constants.
8), wherein each of the spring bodies (117, 118) can be displaced, and the magnitude of the pressing force exerted on the pressure plate (10) by the spring bodies (117, 118) can be changed. apparatus. 2. A container holding section (21) for holding a container (2).
23) and a flexible container (2) containing the liquid fractionated in layers and held in the container holding portions (21, 23).
And a container pressurizing unit (22) for partially extruding and separating the liquid in the container (2), and an interface detecting unit (25) for detecting the interface of the fractionated liquid in the container (2). ), An interface setting unit (50) for setting an interface setting position with respect to the container (2), and a setting result of the interface setting unit (50) and a detection result of the interface detection unit (25). In the liquid separation device (20) including the control unit (26) that can recognize that the detection interface of the interface detection unit (25) has reached the interface setting position, the container pressurizing unit (22) includes: The moving element (113) driven by the pressure driving source (111)
, A pressing plate (10) capable of pressing against the container (2), and an urging device () interposed between the moving element (113) and the pressing plate (10) to apply a pressing force to the pressing plate (10). 114), and the biasing device (114) includes a plurality of spring bodies (117, 118) having different spring constants.
7, 118) can be displaced, and the spring bodies (117, 11) can be displaced.
8) A liquid separation device characterized in that the magnitude of the pressing force exerted on the pressure plate (10) can be changed.