JP2020199256A - Bag set for cord blood and centrifugal machine for cord blood - Google Patents

Abstract

To provide a bag set and a centrifugal machine for mitigating a load of treatment of collecting cord blood stem cells from cord blood.SOLUTION: A bag set 1 comprises a cord blood bag 2 for storing cord blood, a red blood corpuscle bag 3 for storing red blood corpuscles, a stem cell bag 4 for storing cord blood stem cells, and a passage changeover unit 5. A first transfer tube 6 is connected to the cord blood bag 2 and the passage changeover unit 5. A second transfer tube 6 is connected to the red blood corpuscle bag 3 and the passage changeover unit 5. A third transfer tube 7 is connected to the stem cell bag 4 and the passage changeover unit 5. The passage changeover unit 5 is configured to be capable of changing over to a first open state in which the first transfer tube 6 is made to communicate with the second transfer tube 7 and not to communicate with the third transfer tube 8 and a second open state in which the first transfer tube 6 is made mot to communicate with the second transfer tube 7 and to communicate with the third transfer tube 8. A centrifugal machine includes a centrifugal bucket configured to be capable of storing the bag set 1.SELECTED DRAWING: Figure 1

Description

The present invention relates to a bag set and a centrifuge used to collect umbilical cord blood stem cells from umbilical cord blood.

Currently, cord blood stem cells are collected by the following procedure. First, cord blood is collected. There are two methods for collecting umbilical cord blood. One method is predelivery collection when the placenta is in the uterus, and the other method is postpartum collection after the placenta has been removed. In either method, the foetation is detached from the umbilical cord upon delivery and the umbilical cord is clamped to stop bleeding. The needle at the tip of the blood collection tube of the blood collection bag is punctured into the umbilical vein slightly closer to the placenta than the clamped position. Prior to puncture, the area around the puncture site is wiped with sterile gauze and a disinfectant cotton ball to be sufficiently disinfected.

The cord blood is then transferred from the umbilical cord through the blood collection tube to the blood collection bag. At this time, the blood collection bag is held lower than the umbilical cord, and the cord blood is introduced into the blood collection bag by gravity. The blood collection bag contains an anticoagulant in advance.

After the blood flow has stopped, stop the blood collection tube with forceps. Then, the needle at the tip of the blood collection tube is pulled out from the umbilical vein, and a protector is attached. The blood remaining in the blood collection tube is handled with roller pliers and placed in the blood collection bag. After that, the blood collection tube is heat-sealed at a position near the blood collection bag and separated.

The amount of the erythrocyte sedimentation agent added is determined as follows. The total weight of the blood collection bag is measured, the weight of the tare and anticoagulant of the known blood collection bag is subtracted from the total weight to obtain the blood collection amount, and the amount of the red blood cell precipitant added is determined according to the blood collection amount. Then, the determined amount of the erythrocyte sedimentation agent is injected into the blood collection bag, and the mixture is stirred and mixed.

Place the blood collection bag in the centrifuge's centrifugal bucket. To protect the blood collection bag, use spacers to evenly adjust the thickness. Install the bucket in place on the centrifuge. Then, the centrifuge is operated to centrifuge the cord blood in the blood collection bag with a low centrifugal force (centrifugal acceleration) of, for example, about 50 G for a predetermined time to separate the umbilical cord blood into layers. In the blood collection bag, red blood cells precipitate to form a precipitation layer of red blood cells.

Move to a clean bench and set it on the separation stand so as not to disturb the red blood cell precipitate layer in the blood collection bag. A separation bag is prepared separately from the blood collection bag, and the needle at the tip of the transfer tube of the separation bag is pierced into the blood collection bag to connect both bags via the transfer tube.

The separation stand is manually manipulated to extrude leukocytes and plasma above the red blood cell precipitate layer out of the blood collection bag and transfer it through a transfer tube to the separation bag. At this time, since umbilical cord blood stem cells are contained in the upper layer of the erythrocyte precipitation layer of the blood collection bag, several ml of the umbilical cord blood stem cells are also transferred to the separation bag. The amount of transfer depends on the handling conditions and is determined at each facility. After transfer to the separation bag, the clamp of the transfer tube is closed, the transfer tube is heat-sealed on the separation bag side, and the blood collection bag is separated.

Weigh the separation bag to determine the amount of plasma removed.

Put the separation bag in the bucket. To protect the separation bag, use spacers to evenly adjust the thickness. Install the bucket in place on the centrifuge. Then, the centrifuge is operated to centrifuge the cord blood components in the separation bag with a high centrifugal force of, for example, about 400 G for a predetermined time, and the components in the separation bag are separated into the leukocyte layer (lower layer) and the plasma layer (upper layer). Separate into and. Umbilical cord blood stem cells are contained in the leukocyte layer.

Then, the separation bag is set on the separation stand again. The plasma bag is connected to the separation bag via a separate transfer tube. The separation stand is manually manipulated to weigh the determined plasma layer with the removed plasma volume and extrude it from the separation bag, transfer it, and leave an appropriate amount of plasma in the separation bag. After the transfer is complete, the transfer tube clamp is closed, the transfer tube is heat-sealed on the separation bag side, and the plasma bag is detached. Therefore, a leukocyte layer containing umbilical cord blood stem cells is secured in the separation bag.

For freezing storage, the separation bag is weighed again and an appropriate amount of frost damage protective agent is added to the separation bag by injection and mixed. The freezing bag is connected to the separation bag via yet another transfer tube and the contents in the separation bag are gravity transferred to the freezing bag, or the syringe is connected to the separation bag and the contents are aspirated by the syringe. , Pour into a freezing bag, thereby completing the preparation for cryopreservation.

As described above, most of the steps of the process of collecting umbilical cord blood stem cells are performed manually. The total processing time will exceed 6 hours.

Japanese Unexamined Patent Publication No. 2017-070410

Pablo Rubinstein et al .: Processing and cryopreservation of placental / umbilical cord blood for unrelated bone marrow reconstitution. Proc. Natl. Acad. Sci. US A. (1995) Oct.24: Vol.92 (22): 10119-10122. Voluntary Guidelines for Cord Blood Processing and Transplant Indications: 1996 Ministry of Health and Welfare Bone Marrow Transplant Research Project B Edition Examination of cord blood treatment method in cord blood bank: Tsuneo Takahashi et al. Japanese Journal of Transfusion Medicine, 44 (1): 12-19, 1998 Examination of cell recovery by washing frozen cord blood stem cells: Norihiro Sato et al. Japanese Journal of Transfusion Medicine, 44 (6): 687-692, 1998 Cord blood cryopreservation using the automatic cryopreservation device BioArchive: Norihisa Sasayama et al. Japanese Journal of Transfusion Medicine 47 (1): 15-21,2001 Cryopreservation of cord blood stem cells in a small three-dimensional freezing bag: Hideaki Murahashi et al. Medical Instrumentology, 71 (2): 57-62, 2001

An object of the present invention is to provide a cord blood bag set and a cord blood centrifuge that reduce the burden of processing for collecting cord blood stem cells from cord blood.

According to the first aspect of the present invention, a cord blood bag set used for collecting cord blood stem cells from cord blood is provided.
The cord blood bag set is
An umbilical cord blood bag for storing the umbilical cord blood and
A red blood cell bag for storing red blood cells and
A stem cell bag for accommodating the cord blood stem cells and
Flow path switching part and
A first transfer tube connected to the cord blood bag and the flow path switching portion,
A second transfer tube connected to the red blood cell bag and the flow path switching portion,
A third transfer tube connected to the stem cell bag and the flow path switching portion is provided.
The flow path switching unit has a first open state in which the first transfer tube communicates with the second transfer tube and does not communicate with the third transfer tube, and the first transfer tube does not communicate with the second transfer tube. It is configured to be switchable to a second open state in which communication with the third transfer tube is performed.

The flow path switching unit may be further switchable to a closed state in which the first transfer tube is not communicated with both the second transfer tube and the third transfer tube.

The flow path switching portion may be a flow path switching cock.

The cord blood bag comprises an inlet for introducing the cord blood into the cord blood bag.
The portion of the cord blood bag other than the introduction port, the red blood cell bag, the stem cell bag, the flow path switching portion, the first transfer tube, the second transfer tube, and the third transfer tube are for pollution prevention. It is sealed by a film, and the inlet is exposed to the outside of the antifouling film.

According to a second aspect of the present invention, there is provided a cord blood centrifuge used for collecting cord blood stem cells from cord blood.
The cord blood centrifuge is
With the rotor
A rotation mechanism that rotates the rotor around a rotation axis,
A centrifugal bucket supported by the rotor is provided.
The centrifugal bucket is configured to accommodate the bag set according to any one of claims 1 to 4.

The centrifugal bucket is configured to be deployable.
When deployed when the centrifuge bucket houses the cord blood bag set and is supported by the rotor, the red blood cell bag and the stem cell bag may be located lower than the cord blood bag.

The centrifugal bucket
A first container for accommodating the cord blood bag and
A second accommodating portion for accommodating the red blood cell bag and the stem cell bag, which is rotatably provided with respect to the first accommodating portion, is provided.
The centrifugal bucket is deployed by rotating the second accommodating portion with respect to the first accommodating portion.

The cord blood centrifuge may include a deployment mechanism for deploying and locking the centrifuge bucket from deployment.

The cord blood centrifuge may include a switching motor provided in the centrifuge bucket that switches the state of the flow path switching portion.

The cord blood centrifuge is
It comprises at least one optical sensor provided in the centrifuge bucket that detects the optical properties of the cord blood component being transferred within the first transfer tube.
The flow path switching unit may be switched to the first open state or the second open state by the switching motor based on the detection result of the optical sensor.

The cord blood centrifuge is
With the pusher
It is provided with an extrusion mechanism provided in the centrifuge bucket, which presses the pusher against the cord blood bag housed in the centrifuge bucket and pushes a component of the cord blood from the cord blood bag to the first transfer tube. Umbilical cord.

The cord blood centrifuge is
The rotor and the centrifuge bucket supported by the rotor are rotated by the rotation mechanism, and the cord blood is centrifuged in the cord blood bag housed in the centrifuge bucket.
The rotor and the centrifuge bucket supported by the rotor may be further rotated by the rotation mechanism to transfer the centrifuged components of the cord blood from the cord blood bag through the first transfer tube by centrifugal force. ..

The cord blood centrifuge is
A balancer located on the opposite side of the rotation axis from the centrifugal bucket,
An adjustment mechanism provided on the rotor that moves the balancer with respect to the rotor in a direction that approaches the rotation axis and moves away from the rotation axis.
A liquid level sensor provided in the centrifugal bucket, which detects the liquid level in the cord blood bag, is provided.
The balancer may be moved by the adjusting mechanism based on the detection result of the liquid level sensor.

According to the present invention, there is provided a cord blood bag set and a cord blood centrifuge that reduce the burden of processing for collecting cord blood stem cells from cord blood.

It is a front view which shows the cord blood bag set which concerns on one Embodiment. 2A-C are views for explaining the operation of the flow path switching unit. It is a schematic vertical sectional view which shows the centrifuge which concerns on 1st Embodiment. It is a schematic plan view of a centrifuge. It is a top view of a rotor. It is a front view of a centrifugal bucket. FIG. 7A is a side view of the centrifugal bucket, and FIG. 7B is a side sectional view of the centrifugal bucket containing the bag set. It is a front view of the unfolded state of the centrifugal bucket which housed a bag set. It is a side sectional view of the centrifugal bucket containing a bag set in an unfolded state. 10A is a side sectional view of the deployment mechanism, FIG. 10B is a plan view of the deployment mechanism, and FIG. 10C is a side view showing an eccentric cam. 11A is a side view of the centrifugal bucket according to the second embodiment, and FIG. 11B is a side sectional view of the centrifugal bucket of FIG. 11A containing the bag set. It is a front sectional view of the bucket main body part of the centrifugal bucket of FIG. 11 which housed a bag set.

Hereinafter, the cord blood bag set according to the embodiment (hereinafter, simply referred to as a bag set) and the cord blood centrifuge according to the embodiment (hereinafter, simply referred to as a centrifuge) will be described with reference to the drawings. To.

[First Embodiment]
[Bag set]
As shown in FIG. 1, the bag set 1 includes a cord blood bag 2, a red blood cell bag 3, and a stem cell bag 4. The bag set 1 further includes a flow path switching unit 5. The bag set 1 further includes a first transfer tube 6, a second transfer tube 7, and a third transfer tube 8.

The umbilical cord blood bag 2 is for storing umbilical cord blood collected from the umbilical cord. The umbilical cord blood bag 2 includes an introduction port 9 for introducing umbilical cord blood into the bag. The introduction port 9 is provided on the upper side of the cord blood bag 2. The blood collection tube 11 is integrally connected to the introduction port 9 at one end thereof. As will be described later, the cord blood is transferred to and contained in the cord blood bag 2 via the blood collection tube 11. The blood collection tube 11 has a blood collection needle 12 at the other end. Although not shown, the blood collection needle 12 is capped for contamination prevention and accident prevention before use.

The cord blood bag 2 is provided with a sampling port 13 on the upper side thereof. The sampling port 13 is usually sealed by an elastic body and is protected from contamination by a cap 14. A part of the cord blood contained in the cord blood bag 2 can be collected as a sample through the sample collection port 13 by puncturing the elastic body with a needle.

The red blood cell bag 3 is for storing red blood cells. When the cord blood is centrifuged in the cord blood bag 2 by the centrifuge 30 (FIG. 3) as described later, a layer of red blood cells is formed. As will be described later, the red blood cells are transferred from the cord blood bag 2 to the red blood cell bag 3 through the first transfer tube 6 and the second transfer tube 7 and contained.

The stem cell bag 4 is for accommodating cord blood stem cells. When the cord blood is centrifuged in the cord blood bag 2 by the centrifuge 30, a layer of white blood cells containing umbilical cord blood stem cells is formed in addition to the layer of red blood cells. Leukocytes containing the cord blood stem cells are transferred from the cord blood bag 2 to the stem cell bag 4 through the first transfer tube 6 and the third transfer tube 8 and contained.

The stem cell bag 4 is provided with a sampling port 15 on the upper side thereof. The sampling port 15 is usually sealed by an elastic body and is prevented from being contaminated by a cap 16 or the like. A needle can be punctured into an elastic body, and a part of the stem cells contained in the stem cell bag 4 can be collected as a sample through the sample collection port 15.

The first transfer tube 6 is connected to the cord blood bag 2 and the flow path switching portion 5. Specifically, the first transfer tube 6 is connected to the lower side of the cord blood bag 2 and the upper surface of the flow path switching portion 5.

The second transfer tube 7 is connected to the red blood cell bag 3 and the flow path switching unit 5. Specifically, the second transfer tube 7 is connected to the upper side of the red blood cell bag 3 and the lower surface of the flow path switching portion 5.

The third transfer tube 8 is connected to the stem cell bag 4 and the flow path switching portion 5. Specifically, the third transfer tube 8 is connected to the upper side of the stem cell bag 4 and the lower surface of the flow path switching portion 5.

The flow path switching unit 5 is for switching the flow path between the cord blood bag 2, the red blood cell bag 3, and the stem cell bag 4. Specifically, the flow path switching unit 5 is in a closed state in which the first transfer tube 6 is not communicated with both the second transfer tube 7 and the third transfer tube 8, and the first transfer tube 6 is referred to as the second transfer tube 7. It is possible to switch between a first open state in which communication is performed with the third transfer tube 8 and a second open state in which the first transfer tube 6 is communicated with the third transfer tube 8 without communication with the second transfer tube 7. Has been done. The flow path switching unit 5 does not communicate the second transfer tube 7 and the second transfer tube 8 with each other.

The flow path switching unit 5 is a flow path switching cock as shown in FIG. The flow path switching unit 5 includes a case 20. The case 20 has one inlet port 21 and two outlet ports 22, 23. The first transfer tube 6 of the cord blood bag 2 is connected to the inlet port 21. The second transfer tube 7 of the red blood cell bag 3 is connected to one outlet port 22, and the third transfer tube 8 of the stem cell bag 4 is connected to the other outlet port 23.

The flow path switching unit 5 includes a valve body 24 housed in the case 20. The valve body 24 has two passages 25 and 26. These two passages 25 and 26 are formed so as to penetrate the valve body 24.

The flow path switching portion 5 has a valve shaft 27 that is attached to the valve body 24 and penetrates the case 20. The valve shaft 27 is connected to the switching motor 70 (FIG. 7B) of the centrifuge 30 as described later. Therefore, when the switching motor 70 is driven, the valve body 24 and the valve shaft 27 rotate.

As shown in FIG. 2A, when the valve body 24 is in a position where all the ports 21, 22, and 23 are closed, the first transfer tube 6 is not communicated with both the second transfer tube 7 and the third transfer tube 8. Therefore, at this time, the flow path switching unit 5 is in the closed state.

As shown in FIG. 2B, when the valve body 24 communicates the inlet port 21 with one outlet port 22 through one of the passages 25 and closes the other outlet port 23, the first transfer tube 6 is set to the first. 2 Communicates with the transfer tube 7 and does not communicate with the third transfer tube 8. Therefore, at this time, the flow path switching unit 5 is in the first open state.

As shown in FIG. 2C, when the valve body 24 communicates the inlet port 21 with the other outlet port 23 through the other passage 26 and closes one outlet port 22, the first transfer tube 6 is set to the first. 3 Communicates with the transfer tube 8 and does not communicate with the second transfer tube 7. Therefore, at this time, the flow path switching unit 5 is in the second open state.

That is, the valve body 24 is rotated by the switching motor 70 to control its position, so that the flow path switching unit 5 is in the closed state (FIG. 2A), the first open state (FIG. 2B), and the second open state (FIG. 2B). Switch to FIG. 2C).

As shown in FIG. 1, the portion of the cord blood bag 2 excluding the introduction port 9, the red blood cell bag 3, the stem cell bag 4, the flow path switching portion 5, and the transfer tubes 6, 7 and 8 are sealed with the contamination prevention film 10. .. The introduction port 9 and the blood collection tube 11 are exposed to the outside of the contamination prevention film 10.

Although not shown, the cord blood bag 2, the red blood cell bag 3, and the stem cell bag 4 may each include a vent and a vent filter provided at the vent. The ventilation filter is a sterile air vent filter.

Each configuration 2-8 of the bag set 1 is connected in a sealed state, and the cord blood bag 2 to the red blood cell bag 3 and the stem cell bag 4 have a sealed structure.

The cord blood bag 2 has a capacity of 220 ml and a size of 100 mm × 140 mm × 20 mm, the red blood cell bag 3 has a capacity of 120 ml and a size of 80 mm × 130 mm × 12 mm, and the stem cell bag 4 has a capacity of 30 ml, for example. The size is 70 mm × 80 mm × 6 mm.

The material of each component such as each bag 2, 3, 4, each tube 6, 7, 8, and antifouling film 10 is composed of PVC (polyvinyl chloride) or EVA (ethylene / vinyl acetate copolymer). Has been done. Any material may be used as long as it can be handled at a temperature of 4 ° C. or higher and does not elute plasticizers or the like. After each bag 2, 3 and 4, flow path switching part 5, and tubes 6, 7 and 8 are connected and sealed as shown in FIG. 1, EOG sterilization, carbon dioxide gas sterilization, radiation sterilization, ultraviolet sterilization, etc. are performed. It has been sterilized.

[Centrifuge]
3 and 4 schematically show the centrifuge 30. The centrifuge 30 includes a reinforced outer wall 31 and a housing 32 provided on the outer wall 31. Centrifugation of cord blood is performed within the outer wall 31 as described below. As shown in FIG. 3, the discharge port 33 is connected to the lower surface of the outer wall 31, and a stop cock 34 is provided in the discharge port 33 to open and close the discharge port 33. When a liquid or the like leaks, the discharge port 33 discharges the liquid to the outside and then cleans the inside, and the discharge port 33 is for discharging the cleaning liquid.

The centrifuge 30 further includes a control unit 35 (control panel) that controls each operation of the centrifuge 30, and an operation unit 36 (operation panel) that the operator uses to operate the centrifuge 30. The control unit 35 is provided inside the housing 32, and the operation unit 36 is provided on the outer surface of the housing 32. The operation unit 36 receives the information input by the operator and sends it to the control unit 35, and the control unit 35 controls each operation of the centrifuge 30 according to the input information.

The centrifuge 30 includes a rotor 37 and a rotating mechanism 39 that rotates the rotor 37 around its central rotating shaft 38. The rotor 37 is horizontally supported in the outer wall 31 by the rotating mechanism 39. The rotation shaft 38 of the rotor 37 extends in the vertical direction (vertical direction).

The rotation mechanism 39 includes a bearing unit 40 and a centrifugal motor 41. The bearing unit 40 extends vertically between the inside of the outer wall 31 and the inside of the housing 32 through the hole 42. The rotating shaft 38 is rotatably received by the bearing unit 40 and is connected to the centrifugal motor 41. Therefore, when the centrifugal motor 41 is driven, the rotor 37 rotates around the rotation shaft 38. In addition, the rotation mechanism 39 includes a damper 43 and a sealing 44.

The centrifuge 30 further includes a centrifuge bucket 60 that is detachably and rotatably supported by the rotor 37. When the centrifugal bucket 60 is supported by the rotor 37, the centrifugal bucket 60 rotates around the rotation shaft 38 by the rotation mechanism 39 together with the rotor 37. The centrifugal bucket 60 is configured to accommodate the bag set 1 described above. Therefore, the centrifuge bucket 60 containing the bag set 1 is rotated by the rotation mechanism 39, so that the cord blood in the cord blood bag 2 of the bag set 1 can be centrifuged. The structure of the centrifugal bucket 60 will be described later.

As shown in FIGS. 3 and 4, the bucket passage port 45 is formed in the ceiling portion on the upper side of the outer wall 31. A lid 46 (FIG. 3) is rotatably provided on the ceiling portion to open and close the bucket passage port 45. The operator can open the lid 46 and attach the centrifuge bucket 60 to the rotor 37 through the bucket passage 45 and remove it from the rotor 37.

As shown in FIGS. 3 and 5, the centrifuge 30 further includes a balancer 50 and an adjusting mechanism 51 provided on the rotor 37 for moving the balancer 50. The balancer 50 is located on the opposite side of the rotation shaft 38 from the centrifugal bucket 60 supported by the rotor 37. The balancer 50 is provided on the rotor 37 so as to be slidably movable in a direction approaching the rotating shaft 38 and away from the rotating shaft 38.

The adjusting mechanism 51 balances the centrifuge. The adjusting mechanism 51 includes a balancing motor 52 attached to the rotor 37 and a ball screw 53 (FIG. 5) connected to the balancing motor 52. The balancer 50 is screw-engaged with the ball screw 53. Therefore, when the balancing motor 52 is driven and the ball screw 53 is rotated, the balancer 50 moves along the ball screw 53. That is, the balancer 50 moves with respect to the rotor 37 in the direction of approaching the rotating shaft 38 and away from the rotating shaft 38.

As shown in FIGS. 6 and 7, the centrifugal bucket 60 has a first storage portion 61 for storing and holding the cord blood bag 2, and a second storage unit 62 for storing and holding the red blood cell bag 3 and the stem cell bag 4. And.

The first accommodating portion 61 includes a bucket main body 63. The cord blood bag 2 is housed in the bucket body 63. The bucket body 63 has a shape corresponding to the outer shape of the cord blood bag 2, and the cord blood bag 2 is received and held by the bucket body 63. As shown in FIG. 7B, the first transfer tube 6 is housed in the bucket body 63 below the cord blood bag 2. The switching motor 70 is provided in the bucket body 63, and the valve shaft 27 of the flow path switching portion 5 can be connected to the switching motor 70.

As shown in FIG. 7, the first accommodating portion 61 is provided so as to be rotatably provided on the bucket body 63 by a pin 65, for protecting the upper surface cover 66 for covering the bucket body 63 from above and the umbilical cord blood bag 2. A protective plate 67 is further provided. The cord blood bag 2 received by the bucket body 63 is covered with a protective plate 67 to protect it.

As shown in FIG. 6, the first accommodating portion 61 further includes a swing arm 64 that projects horizontally from the left and right outer surfaces of the bucket body 63. The swing arm 64 is located above the bucket body 63. As shown in FIG. 5, the rotor 37 is configured to detachably receive the swing arm 64. The rotor 37 receives the swing arm 64 to swingably support the centrifugal bucket 60 around the swing arm 64. Therefore, when the centrifugal bucket 60 is rotated by the rotation mechanism 39, it receives centrifugal force and swings around the swing arm 64 with respect to the rotor 37.

The second accommodating portion 62 includes a bag cover 68. The red blood cell bag 3 and the stem cell bag 4 are housed in the bag cover 68. Most of the second transfer tube 7 and the third transfer tube 8 are housed in the bag cover 68. The bag cover 68 is rotatably attached to the bucket body 63 at the lower end of the bucket body 63 by a pin 69.

As shown in FIGS. 8 and 9, the centrifugal bucket 60 can be deployed by rotating the second accommodating portion 62 with respect to the first accommodating portion 61. As shown in FIGS. 6-9, the bag cover 68 that partially covers the protective plate 67 and the bucket body 63 rotates downward around the pin 69 to deploy the centrifugal bucket 60.

The cord blood bag 2, the red blood cell bag 3, and the stem cell bag 4 are located at the same height when the undeployed centrifugal bucket 60 houses the bag set 1 and is supported by the rotor 37 (see FIG. 7B). .. When the centrifuge bucket 60 is deployed from this state, the red blood cell bag 3 and the stem cell bag 4 are located lower than the cord blood bag 2 (see FIGS. 8 and 9). Further, at this time, the second transfer tube 7 and the third transfer tube 8 are lowered from the flow path switching portion 5 supported by the bucket body 63. Therefore, the separated components of the cord blood, which will be described later, can be transferred from the cord blood bag 2 to the red blood cell bag 3 or the stem cell bag 4 by gravity.

The centrifuge 30 further comprises a deployment mechanism 90 for deploying and locking the centrifuge bucket 60 (shown only in FIG. 10 and omitted in FIGS. 6-9). 10A is an enlarged view of the region T of FIG. 7B together with the deployment mechanism 90, and FIG. 10B is a plan view of the deployment mechanism 90. As shown in FIG. 10B, the deployment mechanism 90 includes a stopper 91, a deployment motor 92, and an eccentric cam 93 in the first accommodating portion 61. The stopper 91 is rotatably provided around the pin 94. As shown in FIG. 10C, the eccentric cam 93 includes an eccentric protrusion 95, and the eccentric protrusion 95 is inserted into a hole 96 (FIG. 10A) of the stopper 91. The eccentric cam 93 is connected to the deployment motor 92, and when the deployment motor 92 is driven, it rotates around the axis AX (FIG. 10C). Therefore, when the deployment motor 92 is driven, the eccentric protrusion 95 rotates around the axis AX, thereby moving the stopper 91 up and down around the pin 94. As shown in FIG. 10A, when the stopper 91 is lowered and is in contact with the hook 97 at the tip of the bag cover 68, the bag cover 68 is locked by the stopper 91 and does not rotate. Therefore, at this time, as shown in FIG. 7, the centrifugal bucket 60 is locked so as not to expand. On the other hand, from the state of FIG. 10A, when the stopper 91 is raised around the pin 94 by the deployment motor 92, the stopper 91 is separated from the hook 97, the bag cover 68 is unlocked, and as a result, the bag cover 68 is lowered. Rotate to. Therefore, the centrifugal bucket 60 is deployed.

The centrifuge 30 includes a liquid level sensor provided in the centrifugal bucket 60. The liquid level sensor detects the liquid level in the cord blood bag 2.

As shown in FIG. 7B, the liquid level sensor is provided at an appropriate position of the first accommodating portion 61, and is a light source (not shown, for example, an LED) that projects light toward the cord blood bag 2 accommodated in the bucket body 63. ), And a diode array 71 as a detector provided on the inner surface of the bucket body 63 in the vertical direction to detect the light projected from the light source and transmitted or reflected through the cord blood bag 2 (or the liquid inside the cord blood bag 2). Consists of. The diode array 71 may be provided on the protective plate 67 instead of the bucket body 63. Naturally, the cord blood bag 2 is made of a material that is transparent to the light of the light source.

The control unit 35 converts the liquid level detected based on the detection signal of the diode array 71 into the amount of cord blood collected in the cord blood bag 2. The control unit 35 drives the balancing motor 52 and moves the balancer 50 to a position determined according to the sampling amount. Thereby, the balance at the time of centrifugation can be adjusted according to the amount of cord blood collected in the cord blood bag 2.

As shown in FIG. 8, the centrifuge 30 includes two optical sensors 80 provided in the bucket body 63 of the centrifugal bucket 60. The optical sensor 80 detects the optical characteristics of the cord blood (the centrifuged component thereof) moving in the first transfer tube 6. One optical sensor 80 detects optical characteristics on the upstream side and the other optical sensor 80 detects optical characteristics on the downstream side.

The optical sensor 80 may detect either the reflectance or the transmittance of the cord blood component. For example, the optical sensor 80 is provided to detect a light source that projects light toward the first transfer tube 6 and light that is reflected or transmitted by a cord blood component in the first transfer tube 6. It consists of a vessel. As a matter of course, the first transfer tube 6 is made of a material that is transparent to the light of the light source.

The umbilical cord blood is separated into three layers by centrifugation as described later, and the components of the separated layers sequentially flow through the first transfer tube 6. Since the optical characteristics of these three layers are different, the control unit 35 determines which separated layer component is transferred in the first transfer tube 6 based on the signal from the detector of the optical sensor 80. be able to. The control unit 35 can calculate the flow velocity of the cord blood component from the detection time difference between the two optical sensors 80. The control unit 35 drives the switching motor 70 at a timing determined based on the detection results of the two optical sensors 80 to switch the flow path switching unit 5 to the first open state, the second open state, or the open state. .. That is, the control unit 35 can switch the flow path switching unit 5 at a timing that matches the flow velocity of the cord blood component.

As shown in FIG. 5, a power battery 54 and a communication device 55 are provided in the rotor 37. The power battery 54 is electrically connected to the balancing motor 52 and the communication device 55, and supplies electric power to these. When the centrifuge bucket 60 is supported by the rotor 37, a well-known means such as a connector is configured to realize an electrical connection between the centrifuge bucket 60 and the rotor 37. As a result, the power supply battery 54 supplies electric power to the switching motor 70, the deployment motor 92, the liquid level sensor, and the optical sensor 80 in the centrifugal bucket 60. The centrifugal motor 41 is supplied with electric power from another power source instead of the power battery 54.

The communication device 55 has a function of wirelessly communicating with the control unit 35. The signals of the liquid level sensor and the optical sensor 80 are transmitted to the control unit 35 via the communication device 55 and the electrical connection between the centrifugal bucket 60 and the rotor 37 described above. The control unit 35 drives and controls the balancing motor 52 via the communication device 55, and the switching motor 70 and the deployment motor via the electrical connection between the communication device 55 and the centrifugal bucket 60 and the rotor 37 described above. The 92 is driven and controlled. In this way, the control unit 35 can control the motors 52, 70, and 92 provided in the rotor 37 and the centrifugal bucket 60. A part of the control unit 35 may be provided on the rotor 37 to control a part of the operation of the centrifuge 30 without using the communication device 55.

The bag set 1 and the centrifuge 30 constitute a system for collecting cord blood stem cells.

[How to collect cord blood stem cells]
Hereinafter, a method for collecting umbilical cord blood stem cells from umbilical cord blood will be described.

Umbilical cord blood is collected. The bag set 1 shown in FIG. 1 is prepared. The operator punctures the blood collection needle 12 of the blood collection tube 11 into the umbilical vein. The umbilical cord is held higher than the umbilical cord blood bag 2, and the umbilical cord blood is transferred from the umbilical cord through the blood collection tube 11 to the umbilical cord blood bag 2 by gravity and contained. Here, the flow path switching portion 5 is in the closed state (FIG. 2A), and the cord blood in the cord blood bag 2 does not flow to the second transfer tube 7 and the third transfer tube 8. When the bag set 1 is used, an anticoagulant is added to the cord blood bag 2 in advance.

The cord blood is then centrifuged. After collecting blood, the operator heat-seals the blood collection tube 11 at a position near the cord blood bag 2 to separate the blood collection tube 11. In addition, the operator removes the contamination prevention film 10. The operator moves to another location, houses the bag set 1 in the centrifugal bucket 60 as shown in FIG. 7B, and locks the centrifugal bucket 60 with the deploying mechanism 90. Specifically, in the deployed centrifugal bucket 60, the cord blood bag 2 is housed in the bucket body 63, covered with the upper surface cover 66 and the protective plate 67, and the flow path switching portion 5 is connected to the switching motor 70. The red blood cell bag 3 and the stem cell bag 4 are housed in the bag cover 68, and the bag cover 68 is rotated and pressed toward the bucket body 63, and locked by the stopper 91. After that, the centrifugal bucket 60 is supported by the rotor 37.

The operator operates the operation unit 36 of the centrifuge 30 to centrifuge the collected umbilical cord blood into the centrifuge 30. First, the centrifuge 30 detects the liquid level of the cord blood in the cord blood bag 2 by the liquid level sensor, and moves the balancer 50 to an appropriate position by the adjusting mechanism 51 based on the detection result. Then, the centrifuge 30 rotates the rotor 37 and the centrifugal bucket 60 supported by the rotor 37 around the rotating shaft 38 by the rotating mechanism 39. Thereby, the cord blood is centrifuged in the cord blood bag 2. The centrifuge 30 may drive the deployment motor 92 during centrifugation to urge the stopper 91 downward so that the bag cover 68 does not rotate due to centrifugal force.

As a result of centrifugation, the cord blood is separated into three layers in the cord blood bag 2, specifically, roughly divided into three layers. The bottom layer is a layer of red blood cells with a high specific gravity. The middle layer is a layer of white blood cells containing cord blood stem cells. The top layer is the plasma layer.

No erythrocyte sedimentation agent is used in the embodiment. The condition for non-use separation of erythrocyte sedimentation agents is carried out in a centrifugal region where blood cells can be efficiently separated, which is represented by the general formula of sedimentation rate according to Stokes' law (see Equation 1 below).

[Number 1]
(General formula of Stokes' law sedimentation velocity)
Vp = d2 (P1-P2) / 18μ × G
Vp: Sedimentation velocity
d: Particle diameter
P1: Particle density (concentration)
P2: Liquid concentration (density)
μ: Viscosity of liquid
G: Gravity acceleration

Therefore, it is necessary to have a magnitude of 2.5 to 6 times the centrifugal force currently implemented. Moreover, in order to minimize the influence of entrainment by cells having a large specific gravity and a high moving speed, it is necessary to precisely control the rising speed of the centrifugal force. The rotation speed control program of the centrifuge 30 is designed with high control accuracy. For example, the centrifuge 30 centrifuges for about 15 minutes with a centrifugal force (centrifugal acceleration) of about 1800 G.

When the separation and stratification of each cell by centrifugation is completed, the centrifuge 30 decelerates the rotation of the rotor 37 at a speed at which the separation layer is not disturbed, and the centrifugal bucket 60 is locked by the unfolding mechanism 90 shortly before the rotation is stopped. To release. Then, the centrifugal bucket 60 expands toward the outer circumference due to the centrifugal force, but when it goes down, the centrifugal force acts as a brake, and when the rotor 37 is stopped, it expands quietly as shown in FIGS. 8 and 9, and the red blood cell bag 3 and The stem cell bag 4 hangs down below the cord blood bag 2.

The centrifuge 30 drives the switching motor 70 to change the flow path switching unit 5 from the open state to the first open state. Therefore, the first transfer tube 6 and the second transfer tube 7 communicate with each other. As a result, the red blood cells in the lowermost layer in the cord blood bag 2 are transferred to and contained in the red blood cell bag 3 through the first transfer tube 6 and the second transfer tube 7 by gravity.

Following the erythrocytes, leukocytes containing the umbilical cord blood stem cells in the middle layer are transferred within the first transfer tube 6. This is detected by the optical sensor 80. The centrifuge 30 switches the flow path switching unit 5 from the first open state to the second open state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80, and this time the first transfer tube 6 is switched. Communicates with the third transfer tube 8. As a result, leukocytes containing umbilical cord blood stem cells are transferred to the stem cell bag 4 through the third transfer tube 8 and contained.

When the transfer of leukocytes containing umbilical cord blood stem cells is completed, the centrifuge 30 changes the flow path switching unit 5 from the second open state to the closed state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80. Switch. As a result, plasma is collected in the cord blood bag 2 and the first transfer tube 6.

Therefore, the cord blood is separated, the plasma remains in the cord blood bag 2, the red blood cells are contained in the red blood cell bag 3, and the leukocytes containing the cord blood stem cells are contained in the stem cell bag 4. In this way, the collection of cord blood stem cells is completed. When the centrifuge 30 completes the centrifugation and the subsequent storage of the cord blood stem cells in the stem cell bag 4, the operator is notified by voice or display to that effect.

The method for collecting stem cells according to the above embodiment has the following advantages.

While the conventional collection method is completed in about 6 hours, the collection method of the embodiment of the present application is completed in about 60 minutes, which greatly reduces the time burden. In the conventional collection process, most of the work is performed manually, and the operator is forced to perform a tense work for a long time. On the other hand, in the sampling process of the embodiment of the present application, most of the work is automatically performed by the bag set 1 and the centrifuge 30, so that the labor burden on the operator is greatly reduced.

The sampling method of the embodiment of the present application reduces the work of the following conventional sampling method.
-Drug addition and mixing work for red blood cell precipitation (not required at all in this application)
-Two-degree centrifugation performed by switching between low-centrifugal force and high-centrifugal force (one-degree centrifugation in this application)
・ Work to transfer the upper layer other than erythrocytes from the blood collection bag to the separation bag ・ Work to transfer the upper layer other than the cord blood stem cells from the separation bag to the plasma bag ・ Work to transfer or transfer the cord blood stem cells from the separation bag to the stem cell bag ・Repeated weighing operations during processing operations

Each component of the bag set 1 is hermetically connected so that cord blood does not come into contact with the external environment and the potential for contamination is minimized. The contamination prevention film 10 prevents contamination of the bag set 1 at the time of blood collection.

The amount of umbilical cord blood collected varies from individual to individual. This difference impairs the balance during centrifugation and poses a risk of breakage. However, the balancer 50, the adjusting mechanism 51, and the liquid level sensor ensure an appropriate balance according to the collected amount and ensure stable centrifugation.

Cord blood is separated by high centrifugal force. Since the first accommodating portion 61 of the centrifuge bucket 60 has a shape that matches the outer shape of the cord blood bag 2 and receives the cord blood bag 2, damage due to deformation of the cord blood bag 2 is prevented. ..

Sterile air vent filters provided in each of the cord blood bag 2, the red blood cell bag 3, and the stem cell bag 4 reduce the resistance generated during the transfer of the cord blood components, eliminate the change in internal pressure of each bag, and transfer. I'm sure. Even a bag set without this sterilized air vent filter can be put into practical use if the internal pressure is lowered or the internal capacity is reduced when the bag set is set. For example, like a vacuum blood collection tube, the pressure inside the three bags 2, 3 and 4 is appropriately reduced to take in umbilical cord blood, and after centrifugation, the flow path switching unit 5 is used to reduce the red blood cell layer and the white blood cell layer containing stem cells. And may be transferred without disturbance of the layer.

The difference in detection time between the two optical sensors 80 is converted into a flow velocity to ensure the timing accuracy of the start and end of transfer to the red blood cell bag 3 and the stem cell bag 4. That is, the accuracy is improved by correcting the difference in viscosity due to the temperature, concentration, etc. of blood during treatment. As a result, cord blood stem cells can be collected accurately and efficiently.

In the bag set 1 of FIG. 1, the blood collection tube 11 is integrally connected to the cord blood bag 2. Instead, the blood collection tube 11 may be provided separately from the cord blood bag 2 before use.
For example, the cord blood bag 2 has an introduction port 9 and a contamination prevention cap (not shown) that is detachably attached to the introduction port 9 to seal the introduction port 9, and a blood collection tube 11 has a blood collection needle at one end. It may have a shape having 12 and being connected to the introduction port 9 at the other end. When collecting umbilical cord blood, the anticontamination cap is removed, the blood collection tube 11 is connected to the introduction port 9, and the blood collection needle 12 is punctured into the umbilical cord. After blood collection, the blood collection tube 11 is removed from the introduction port 9, and a contamination prevention cap is attached to the introduction port 9. Also in this case, both ends of the blood collection tube 11 are capped before use.

Hereinafter, another embodiment will be described. The same or similar configurations as those in the first embodiment are designated by the same reference numerals, and the description thereof will be omitted. Also, steps of the same or similar method as in the first embodiment are omitted as much as possible.

[Second Embodiment]
11 and 12 show the centrifugal bucket 60 according to the second embodiment. The bag set 1 is housed in the bucket body 63. That is, both the first accommodating portion 61 for accommodating the cord blood bag 2 and the second accommodating portion 62 for accommodating the red blood cell bag 3 and the stem cell bag 4 are formed by the bucket body 63. The second accommodating portion 62 is provided below the first accommodating portion 61 and the swing arm 64. The cord blood bag 2 is housed vertically, and the red blood cell bag 3 and the stem cell bag 4 are housed horizontally. The centrifugal bucket 60 is closed by covering the bucket body 63 with the bag cover 68 and then covering the bucket body 63 with the top cover 66.

The centrifuge 30 further includes a pusher 100 and an extrusion mechanism 102 provided in the centrifuge bucket 60 that presses the pusher 100 against the cord blood bag 2 housed in the centrifuge bucket 60.

The pusher 100 has a plate shape. The pusher 100 is rotatably attached to the bag cover 68 by a pin 101 at its lower end. As shown in FIG. 11B, when the bag cover 68 covers the bucket body 63 in which the bag set 1 is housed, the pusher 100 faces the cord blood bag 2. Therefore, the cord blood bag 2 is sandwiched between the pusher 100 and the inner wall of the centrifugal bucket 60 (bucket body 63).

As shown in FIG. 11B, the extrusion mechanism 102 includes an extrusion motor 103. The extrusion motor 103 is attached to the upper part of the bucket body 63 so that its shaft 104 is horizontal. The extrusion motor 103 is configured to be powered by the power battery 54 (FIG. 5) and controlled by the control unit 35 (FIG. 3).

A mounting body 105 having a threaded outer peripheral surface is fitted to the shaft 104. A hole is formed at the upper end of the pusher 100. As shown in FIG. 11B, when the bag cover 68 covers the bucket body 63, the pusher 100 can be threaded on the mounting body 104 at the position of the hole and mounted on the mounting body 104. In this state, if the extrusion mechanism 102 drives the motor 103 to rotate the shaft 104, the pusher 100 can be moved around the pin 101 within a predetermined range. Therefore, the extrusion mechanism 102 can press the pusher 100 against the cord blood bag 2.

By pressing the pusher 100 against the cord blood bag 2, the extrusion mechanism 102 can sandwich the cord blood bag 2 between the pusher 100 and the inner wall of the centrifugal bucket 60, whereby the volume of the cord blood bag 2 can be continuously increased. It can be forcibly reduced. That is, the extrusion mechanism 102 can push the component of the cord blood from the cord blood bag 2 to the first transfer tube 6 by the pusher 100 and transfer it.

The extrusion mechanism 102 is not limited to the above configuration, and any configuration can be adopted as long as the pusher 100 can be moved to push out the cord blood component from the cord blood bag 2 by the pusher 100. May be done. For example, the pusher 100 is arranged on the opposite side of the bag cover 68 so that the extrusion mechanism 102 presses the pusher 100 against the cord blood bag 2 so that the cord blood bag 2 is sandwiched between the pusher 100 and the inner wall of the bag cover 68. The cord blood component may be extruded from the cord blood bag 2.

Hereinafter, in the second embodiment, a method for collecting umbilical cord blood stem cells from umbilical cord blood will be described. In this embodiment, when the bag set 1 is housed in the bucket body 63 and the bucket body 63 is covered with the bag cover 68, the pusher 100 is hung on the mounting body 104 and mounted as described above. Then, as in the first embodiment, the centrifuge 30 centrifuges the cord blood in the cord blood bag 2 and divides the cord blood into three layers.

Next, the centrifuge 30 drives the switching motor 70 to open the flow path switching unit 5 from the open state to the first open state, and communicates the first transfer tube 6 with the second transfer tube 7. Then, the centrifuge 30 drives the extrusion motor 103 to press the pusher 100 against the cord blood bag 2 to reduce the volume of the cord blood bag 2. As a result, the red blood cells in the lowermost layer are pushed out from the cord blood bag 2, transferred to the red blood cell bag 3 through the first transfer tube 6 and the second transfer tube 7, and contained.

Next, the centrifuge 30 switches the flow path switching unit 5 from the first open state to the second open state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80, and the first transfer tube 6 This time communicates with the third transfer tube 8. The centrifuge 30 presses the pusher 100 against the cord blood bag 2 and continues to reduce the volume of the cord blood bag 2. Therefore, leukocytes containing cord blood stem cells are extruded from the cord blood bag 2 and transferred to the stem cell bag 4 through the first transfer tube 6 and the third transfer tube 8 and are contained therein.

Next, the centrifuge 30 switches the flow path switching unit 5 from the second open state to the closed state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80. As a result, plasma is collected in the cord blood bag 2 and the first transfer tube 6. The centrifuge 30 stops pressing the pusher 100 against the cord blood bag 2.

Thus, as in the previous embodiment, the collection of cord blood stem cells is completed.

In the second embodiment, the transfer rate of the cord blood component can be adjusted by adjusting the extrusion rate of the pusher 100. That is, the components of cord blood can be transferred at an arbitrary speed from a slow speed of free fall or less to a speed several times higher than that. In the centrifuge 30 of the second embodiment, more efficient collection of stem cells from umbilical cord blood can be realized by selecting the optimum transfer rate.

Further, it is an advantage of the second embodiment that the deployment mechanism 90 is not required and the structure of the centrifugal bucket 60 is simplified as compared with the first embodiment.

[Third Embodiment]
In the third embodiment, the transfer of the cord blood component is performed by utilizing centrifugal force. In this embodiment, for example, the centrifugal bucket 60 as shown in FIG. 11 without the pusher 100 and the extrusion mechanism 102 is used. Hereinafter, a method for collecting umbilical cord blood stem cells from umbilical cord blood according to the third embodiment will be described.

Similar to the previous embodiment, the centrifuge 30 rotates the rotor 37 and the centrifuge bucket 60 supported by the rotor 37 around the rotating shaft 38 by the rotating mechanism 39 to centrifuge the cord blood in the cord blood bag 2. To separate.

Next, the centrifuge 30 changes the flow path switching portion 5 from the open state to the first open state by the switching motor 70, and causes the rotor 37 and the centrifugal bucket 60 supported by the rotor 37 around the rotating shaft 38 by the rotating mechanism 39. Rotate and apply additional centrifugal force to the centrifuged umbilical cord blood. As a result, the red blood cells in the lowermost layer are transferred from the cord blood bag 2 to the red blood cell bag 3 through the first transfer tube 6 and the second transfer tube 7 by centrifugal force and are contained therein.

Next, the centrifuge 30 switches the flow path switching unit 5 from the first open state to the second open state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80. The rotor 37 and the centrifugal bucket 60 supported by the rotor 37 continue to rotate. Therefore, leukocytes containing cord blood stem cells are transferred from the cord blood bag 2 to the stem cell bag 4 through the first transfer tube 6 and the third transfer tube 8 by centrifugal force and are contained therein.

Next, the centrifuge 30 switches the flow path switching unit 5 from the second open state to the closed state by the switching motor 70 at a timing determined based on the detection result of the optical sensor 80, and further stops the rotation of the rotor 37. .. As a result, plasma is collected in the cord blood bag 2 and the first transfer tube 6. In this way, the collection of cord blood stem cells is completed.

In the third embodiment, the components of the umbilical cord blood are transferred by utilizing the original function of the centrifuge 30 to apply centrifugal force. Therefore, the expansion mechanism 90, the pusher 100, and the extrusion mechanism 102 as described above are unnecessary. Thereby, further structural rationalization of the centrifugal bucket 60, and thus the centrifuge 30, can be realized.

Since the transfer is performed using the centrifugal force, it is also an advantage of the third embodiment that the cord blood component can be transferred at a desired transfer rate by accurately controlling the centrifugal force and therefore the rotation speed of the rotor 37. is there.

1 Umbilical cord blood bag set 2 Umbilical cord blood bag 3 Red blood cell bag 4 Stem cell bag 5 Flow path switching part (flow path switching cock)
6 1st transfer tube 7 2nd transfer tube 8 3rd transfer tube 9 Inlet port 10 Contamination prevention film 30 Centrifuge 37 Rotor 38 Rotating shaft 39 Rotating mechanism 50 Balancer 51 Adjusting mechanism 60 Centrifugal bucket 61 1st accommodating part 62 2nd Containment unit 70 Switching motor 71 Diode array 80 Optical sensor 90 Deployment mechanism 100 Pusher 102 Extrusion mechanism

Claims (13)

A cord blood bag set used to collect umbilical cord blood stem cells from umbilical cord blood.
An umbilical cord blood bag for storing the umbilical cord blood and
A red blood cell bag for storing red blood cells and
A stem cell bag for accommodating the cord blood stem cells and
Flow path switching part and
A first transfer tube connected to the cord blood bag and the flow path switching portion,
A second transfer tube connected to the red blood cell bag and the flow path switching portion,
A third transfer tube connected to the stem cell bag and the flow path switching portion is provided.
The flow path switching unit has a first open state in which the first transfer tube communicates with the second transfer tube and does not communicate with the third transfer tube, and the first transfer tube does not communicate with the second transfer tube. It is configured to be switchable to a second open state in which it communicates with the third transfer tube.
Umbilical cord blood bag set featuring that. The flow path switching unit is configured to be further switchable to a closed state in which the first transfer tube is not communicated with both the second transfer tube and the third transfer tube.
The umbilical cord blood bag set according to claim 1. The flow path switching portion is a flow path switching cock.
The umbilical cord blood bag set according to claim 1 or 2. The cord blood bag
Provided with an inlet for introducing the cord blood into the cord blood bag.
The portion of the cord blood bag other than the introduction port, the red blood cell bag, the stem cell bag, the flow path switching portion, the first transfer tube, the second transfer tube, and the third transfer tube are for pollution prevention. Sealed by a film, the inlet is exposed to the outside of the antifouling film.
The cord blood bag set according to any one of claims 1 to 3, wherein the umbilical cord blood bag set. An umbilical cord blood centrifuge used to collect umbilical cord blood stem cells from umbilical cord blood.
With the rotor
A rotation mechanism that rotates the rotor around a rotation axis,
A centrifugal bucket supported by the rotor is provided.
The centrifugal bucket is configured to accommodate the bag set according to any one of claims 1 to 4.
A centrifuge for umbilical cord blood. The centrifugal bucket is configured to be deployable.
When deployed while the centrifuge bucket houses the cord blood bag set and is supported by the rotor, the red blood cell bag and the stem cell bag are located lower than the cord blood bag.
The umbilical cord blood centrifuge according to claim 5. The centrifugal bucket
A first container for accommodating the cord blood bag and
A second accommodating portion for accommodating the red blood cell bag and the stem cell bag, which is rotatably provided with respect to the first accommodating portion, is provided.
The centrifugal bucket is deployed by rotating the second accommodating portion with respect to the first accommodating portion.
The umbilical cord blood centrifuge according to claim 6. A deployment mechanism for deploying and locking the centrifugal bucket from deploying.
The umbilical cord blood centrifuge according to claim 6 or 7. A switching motor provided in the centrifugal bucket for switching the state of the flow path switching unit is provided.
The umbilical cord blood centrifuge according to any one of claims 5 to 8, characterized in that. It comprises at least one optical sensor provided in the centrifuge bucket that detects the optical properties of the cord blood component being transferred within the first transfer tube.
The flow path switching unit is switched to the first open state or the second open state by the switching motor based on the detection result of the optical sensor.
The umbilical cord blood centrifuge according to claim 9. With the pusher
It is provided with an extrusion mechanism provided in the centrifuge bucket, which presses the pusher against the cord blood bag housed in the centrifuge bucket and pushes a component of the cord blood from the cord blood bag to the first transfer tube. ,
The umbilical cord blood centrifuge according to claim 5. The cord blood centrifuge is
The rotor and the centrifuge bucket supported by the rotor are rotated by the rotation mechanism, and the cord blood is centrifuged in the cord blood bag housed in the centrifuge bucket.
The rotor and the centrifuge bucket supported by the rotor are further rotated by the rotation mechanism, and the centrifugally separated components of the cord blood are transferred from the cord blood bag through the first transfer tube by centrifugal force.
The umbilical cord blood centrifuge according to claim 5. A balancer located on the opposite side of the rotation axis from the centrifugal bucket,
An adjustment mechanism provided on the rotor that moves the balancer with respect to the rotor in a direction that approaches the rotation axis and moves away from the rotation axis.
A liquid level sensor provided in the centrifugal bucket, which detects the liquid level in the cord blood bag, is provided.
The balancer is moved by the adjusting mechanism based on the detection result of the liquid level sensor.
The cord blood centrifuge according to any one of claims 5 to 12, characterized in that.

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