JP2547264C - - Google Patents
Info
- Publication number
- JP2547264C JP2547264C JP2547264C JP 2547264 C JP2547264 C JP 2547264C JP 2547264 C JP2547264 C JP 2547264C
- Authority
- JP
- Japan
- Prior art keywords
- factor
- polyglycoside
- sample
- inhibitor
- activation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000002401 inhibitory effect Effects 0.000 claims description 95
- 239000003112 inhibitor Substances 0.000 claims description 76
- 230000004913 activation Effects 0.000 claims description 62
- 239000002158 endotoxin Substances 0.000 claims description 40
- 229920002498 Beta-glucan Polymers 0.000 claims description 38
- WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims description 28
- 239000006166 lysate Substances 0.000 claims description 24
- 241000239218 Limulus Species 0.000 claims description 23
- 241000239220 Limulus polyphemus Species 0.000 claims description 21
- 229920002558 Curdlan Polymers 0.000 claims description 20
- 239000001879 Curdlan Substances 0.000 claims description 20
- 235000019316 curdlan Nutrition 0.000 claims description 20
- 229940078035 curdlan Drugs 0.000 claims description 20
- 239000012190 activator Substances 0.000 claims description 14
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 12
- OZAIFHULBGXAKX-UHFFFAOYSA-N precursor Substances N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 claims description 7
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L MgCl2 Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 108010065152 Coagulase Proteins 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007792 addition Methods 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims 2
- 239000000523 sample Substances 0.000 description 73
- 238000002360 preparation method Methods 0.000 description 57
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 42
- 239000000243 solution Substances 0.000 description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 26
- 238000005194 fractionation Methods 0.000 description 20
- 239000012153 distilled water Substances 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 239000000126 substance Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 description 11
- FYGDTMLNYKFZSV-WFYNLLPOSA-N (2S,3R,4S,5S,6R)-2-[(2R,4R,5R,6S)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2R,3S,4R,5R,6S)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-WFYNLLPOSA-N 0.000 description 11
- 229920001503 Glucan Polymers 0.000 description 11
- 238000006467 substitution reaction Methods 0.000 description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 10
- 229960001031 Glucose Drugs 0.000 description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- 150000008131 glucosides Chemical group 0.000 description 10
- 229920001543 Laminarin Polymers 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 239000008103 glucose Substances 0.000 description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 8
- 239000002253 acid Substances 0.000 description 8
- 230000015271 coagulation Effects 0.000 description 8
- 238000005345 coagulation Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 239000002808 molecular sieve Substances 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 6
- 238000001879 gelation Methods 0.000 description 6
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 6
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000209219 Hordeum Species 0.000 description 5
- 235000007340 Hordeum vulgare Nutrition 0.000 description 5
- -1 amine salts Chemical class 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000000502 dialysis Methods 0.000 description 5
- 238000000527 sonication Methods 0.000 description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 239000005695 Ammonium acetate Substances 0.000 description 4
- 206010017533 Fungal infection Diseases 0.000 description 4
- 241001466453 Laminaria Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 4
- 235000019257 ammonium acetate Nutrition 0.000 description 4
- 229940043376 ammonium acetate Drugs 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 4
- 238000006116 polymerization reaction Methods 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 241001474374 Blennius Species 0.000 description 3
- 210000002421 Cell Wall Anatomy 0.000 description 3
- 206010067410 Endotoxaemia Diseases 0.000 description 3
- 241000199919 Phaeophyceae Species 0.000 description 3
- 241000239224 Tachypleus tridentatus Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 3
- 239000012523 bacterial endotoxin Substances 0.000 description 3
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 201000009910 diseases by infectious agent Diseases 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- BDAGIHXWWSANSR-UHFFFAOYSA-N formic acid Chemical compound OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 3
- 230000002538 fungal Effects 0.000 description 3
- 230000002458 infectious Effects 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000004879 turbidimetry Methods 0.000 description 3
- KRCLQJBBBGFWTC-OMCNKCBHSA-N (1R,3aS,5aR,5bR,9S,11aR)-9-[2-[methoxy-[(3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]amino]acetyl]oxy-5a,5b,8,8,11a-pentamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-3a-carboxylic acid Chemical compound O([C@@H]1C(C2[C@](C3[C@@]([C@@]4(CC[C@]5(CC[C@H](C5C4CC3)C(C)=C)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(=O)CN(OC)C1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O KRCLQJBBBGFWTC-OMCNKCBHSA-N 0.000 description 2
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-Nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 2
- 241000588986 Alcaligenes Species 0.000 description 2
- 206010060945 Bacterial infection Diseases 0.000 description 2
- 210000004369 Blood Anatomy 0.000 description 2
- 210000000601 Blood Cells Anatomy 0.000 description 2
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 2
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- 241000239219 Carcinoscorpius rotundicauda Species 0.000 description 2
- 241000230129 Eisenia <Phaeophyceae> Species 0.000 description 2
- MZNYWPRCVDMOJG-UHFFFAOYSA-N N-(1-naphthyl)ethylenediamine dihydrochloride Chemical compound [Cl-].[Cl-].C1=CC=C2C([NH2+]CC[NH3+])=CC=CC2=C1 MZNYWPRCVDMOJG-UHFFFAOYSA-N 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N Perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 229920000297 Rayon Polymers 0.000 description 2
- 206010038444 Renal failure chronic Diseases 0.000 description 2
- 241000222480 Schizophyllum Species 0.000 description 2
- 241000221662 Sclerotinia Species 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M Sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- AKEJUJNQAAGONA-UHFFFAOYSA-N Sulfur trioxide Chemical compound O=S(=O)=O AKEJUJNQAAGONA-UHFFFAOYSA-N 0.000 description 2
- 241000239221 Tachypleus gigas Species 0.000 description 2
- 231100000765 Toxin Toxicity 0.000 description 2
- QWDZADMNIUIMTC-UHFFFAOYSA-N Uranyl nitrate Chemical compound [O-][N+](=O)O[U-2](=O)(=O)O[N+]([O-])=O QWDZADMNIUIMTC-UHFFFAOYSA-N 0.000 description 2
- 241000006302 Usnea Species 0.000 description 2
- 230000002378 acidificating Effects 0.000 description 2
- 230000003213 activating Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 239000003114 blood coagulation factor Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cells Anatomy 0.000 description 2
- 229940079593 drugs Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Polymers 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 239000002964 rayon Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 239000004627 regenerated cellulose Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001225 therapeutic Effects 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- UUQINGLDFWYORW-RJVMBZMCSA-N (2R,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,5-dihydroxy-6-(hyd Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H]([C@H](O[C@@H]3[C@H]([C@H](O[C@H]4[C@@H]([C@@H](CO)O[C@@H](O)[C@@H]4O)O)O[C@H](CO)[C@H]3O)O)O[C@H](CO)[C@H]2O)O)O[C@H](CO)[C@H]1O UUQINGLDFWYORW-RJVMBZMCSA-N 0.000 description 1
- KDUMGDGPRAORNS-ODKLGZGOSA-N (2R,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxym Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H]([C@H](O[C@@H]3[C@H]([C@H](O[C@@H]4[C@H]([C@H](O[C@@H]5[C@H]([C@H](O[C@H]6[C@@H]([C@@H](CO)O[C@@H](O)[C@@H]6O)O)O[C@H](CO)[C@H]5O)O)O[C@H](CO)[C@H]4O)O)O[C@H](CO)[C@H]3O)O)O[C@H](CO)[C@H]2O)O)O[C@H](CO)[C@H]1O KDUMGDGPRAORNS-ODKLGZGOSA-N 0.000 description 1
- SPMCUTIDVYCGCK-IIIGWGBSSA-N (2S,3R,4S,5R,6R)-2-[(2R,3R,4R,5S)-3,5-dihydroxy-2-(hydroxymethyl)oxan-4-yl]oxy-4-[(2S,3R,5S,6R)-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-(hydroxymethyl)oxane-3,5-diol Chemical compound O[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)OC[C@@H]2O)O)O[C@H](CO)[C@H]1O SPMCUTIDVYCGCK-IIIGWGBSSA-N 0.000 description 1
- OLBQXBHLZOAVSV-UKPDYPBGSA-N (3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-4-[(2S,3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H]([C@H](O[C@H]3[C@@H]([C@@H](CO)OC(O)[C@@H]3O)O)O[C@H](CO)[C@H]2O)O)O[C@H](CO)[C@H]1O OLBQXBHLZOAVSV-UKPDYPBGSA-N 0.000 description 1
- XJKJWTWGDGIQRH-BFIDDRIFSA-N Alginic acid Chemical compound O1[C@@H](C(O)=O)[C@@H](OC)[C@H](O)[C@H](O)[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](C)[C@@H](O)[C@H]1O XJKJWTWGDGIQRH-BFIDDRIFSA-N 0.000 description 1
- GEHMBYLTCISYNY-UHFFFAOYSA-N Ammonium sulfamate Chemical compound [NH4+].NS([O-])(=O)=O GEHMBYLTCISYNY-UHFFFAOYSA-N 0.000 description 1
- 102000037197 Anion exchangers Human genes 0.000 description 1
- 108091006437 Anion exchangers Proteins 0.000 description 1
- 241000206761 Bacillariophyta Species 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 206010006473 Bronchopulmonary aspergillosis Diseases 0.000 description 1
- 229920000018 Callose Polymers 0.000 description 1
- 241001310323 Cetraria Species 0.000 description 1
- 241000227752 Chaetoceros Species 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N Chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 208000009863 Chronic Kidney Failure Diseases 0.000 description 1
- 208000001458 Chronic Renal Insufficiency Diseases 0.000 description 1
- 241001529717 Corticium <basidiomycota> Species 0.000 description 1
- 241001466512 Coscinodiscus Species 0.000 description 1
- YPHMISFOHDHNIV-FSZOTQKASA-N Cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 241000243681 Eisenia bicyclis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000195620 Euglena Species 0.000 description 1
- 241000004871 Evernia Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000012480 LAL reagent Substances 0.000 description 1
- 229920001491 Lentinan Polymers 0.000 description 1
- 241000222418 Lentinus Species 0.000 description 1
- 210000002751 Lymph Anatomy 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000199478 Ochromonas Species 0.000 description 1
- 229920002984 Paramylon Polymers 0.000 description 1
- 241000206731 Phaeodactylum Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 210000002381 Plasma Anatomy 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241001302191 Polyphemus Species 0.000 description 1
- 241001619461 Poria <basidiomycete fungus> Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 208000004430 Pulmonary Aspergillosis Diseases 0.000 description 1
- 101710033092 SP41A Proteins 0.000 description 1
- 229920002305 Schizophyllan Polymers 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 241000206733 Skeletonema Species 0.000 description 1
- 241000932081 Stromatinia Species 0.000 description 1
- 101700038834 TLP1A Proteins 0.000 description 1
- 101700051075 TULP1 Proteins 0.000 description 1
- 240000008529 Triticum aestivum Species 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 238000006149 azo coupling reaction Methods 0.000 description 1
- 230000001580 bacterial Effects 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 101710022772 bgc Proteins 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 108010071063 butyloxycarbonyl-leucyl-glycyl-arginine-4-nitroanilide Proteins 0.000 description 1
- 125000004181 carboxyalkyl group Chemical group 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 108010055222 clotting enzyme Proteins 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 230000004059 degradation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- TXKMVPPZCYKFAC-UHFFFAOYSA-N disulfur monoxide Inorganic materials O=S=S TXKMVPPZCYKFAC-UHFFFAOYSA-N 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 239000012525 endotoxin sample Substances 0.000 description 1
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 description 1
- 238000006266 etherification reaction Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000001631 haemodialysis Methods 0.000 description 1
- 230000000322 hemodialysis Effects 0.000 description 1
- 108010076951 horseshoe crab coagulation factor G Proteins 0.000 description 1
- 125000004435 hydrogen atoms Chemical group [H]* 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- DBTMGCOVALSLOR-OGEYSLCJSA-N laminaritriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)OC(O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-OGEYSLCJSA-N 0.000 description 1
- 229940115286 lentinan Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 238000003541 multi-stage reaction Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 230000003389 potentiating Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001226 reprecipitation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005670 sulfation reaction Methods 0.000 description 1
- XTQHKBHJIVJGKJ-UHFFFAOYSA-N sulfur monoxide Chemical compound S=O XTQHKBHJIVJGKJ-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 235000021307 wheat Nutrition 0.000 description 1
Family
ID=
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU625098B2 (en) | Limulus amoebocyte lysate g-factor activation | |
| KR0141685B1 (ko) | 참게 아메보사이트 라이세이트 g 인자 활성화 저해제 | |
| Kim et al. | Structural characterization of β-D-(1→ 3, 1→ 6)-linked glucans using NMR spectroscopy | |
| JP3122454B2 (ja) | カブトガニ・アメボサイト・ライセートの調製法 | |
| Woodward et al. | Water-soluble (1→ 3),(1→ 4)-β-D-glucans from barley (Hordeum vulgare) endosperm. II. Fine structure | |
| Kitamura et al. | An antitumor, branched (1→ 3)-β-D-glucan from a water extract of fruiting bodies of Cryptoporus volvatus | |
| EP2935339B1 (en) | Preparation of poly alpha-1,3-glucan ethers | |
| US9139718B2 (en) | Preparation of poly alpha-1,3-glucan ethers | |
| Bardalaye et al. | Galactosaminogalactan from cell walls of Aspergillus niger | |
| CN102367285B (zh) | 菲律宾刺参硫酸软骨素多糖及其提取方法和用途 | |
| Gong et al. | Structural characterization and immunostimulatory activity of a glucan from Cyclina sinensis | |
| Beigi et al. | A water-soluble polysaccharide from the roots of Eremurus spectabilis MB subsp. spectabilis: Extraction, purification and structural features | |
| Brandi et al. | Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated ($1 {\rightarrow} 3$)($1 {\rightarrow} 6$)-${\beta} $-D-Glucan | |
| JP4071438B2 (ja) | 真菌からの（１→３）−β−Ｄ−グルカンの調製法 | |
| EP1021463B1 (en) | Factor g reduced amebocyte lysates for detection of bacterial endotoxins | |
| Niu et al. | Preparation and sulfation of an α-glucan from Actinidia chinensis roots and their potential activities | |
| JP2547264C (ko) | ||
| JP2547264B2 (ja) | カブトガニ・アメボサイト・ライセートｇ因子活性化阻害剤 | |
| Jamas et al. | Spectral analysis of glucan produced by wild-type and mutant Saccharomyces cerevisiae | |
| Yamada et al. | Characterisation of fucomannopeptide and mannoprotein from Absidia cylindrospora | |
| CN109323996B (zh) | 一种真菌检测试剂盒 | |
| JPH02186267A (ja) | カブトガニ・アメボサイト・ライセートｇ因子活性化阻害剤 | |
| CN108976313B (zh) | 一种多聚葡萄糖醛酸及其制备方法和在免疫调节药物中的应用 | |
| Ludwig-Baxter et al. | A novel method for differentiating dextran sulfate from related sulfated polysaccharides | |
| Chen et al. | Structural characterization of the carbohydrate portion of a glycopeptide from wheat gluten |