JP2531679B2 - Topical - Google Patents
TopicalInfo
- Publication number
- JP2531679B2 JP2531679B2 JP62137072A JP13707287A JP2531679B2 JP 2531679 B2 JP2531679 B2 JP 2531679B2 JP 62137072 A JP62137072 A JP 62137072A JP 13707287 A JP13707287 A JP 13707287A JP 2531679 B2 JP2531679 B2 JP 2531679B2
- Authority
- JP
- Japan
- Prior art keywords
- malt root
- extract
- added
- malt
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は麦芽根の水抽出物を含有した線維芽細胞増殖
作用及び創傷治癒作用を有する外用剤に関するものであ
る。TECHNICAL FIELD The present invention relates to an external preparation containing a water extract of malt root and having a fibroblast proliferation action and a wound healing action.
麦芽は大麦に水分と温度と酸素を作用させて発芽させ
た麦粒であり、発芽した大麦の中には強いアミラーゼが
できるので、これを澱粉の分解に応用して、水飴の製
造、ビールの醸造に広く用いられている。Malt is a barley grain that is germinated by the action of water, temperature and oxygen on barley, and strong amylase is formed in the germinated barley, so this is applied to the decomposition of starch to produce starch syrup and beer Widely used in brewing.
しかるに、この麦芽の根の部分、即ち麦芽根は利用性
がなく、現在、水飴，ビールの製造の場合は麦芽根を切
除して使用している。従って、麦芽根はそのまま廃棄さ
れてその利用法は殆ど開発されていない。However, this malt root part, that is, the malt root is not available, and in the case of manufacturing starch syrup and beer, the malt root is cut off and used at present. Therefore, malt root is discarded as it is, and its utilization method has hardly been developed.
本発明は上述の如く、現在殆ど利用性がなく、麦芽の
使用のため多大の量が廃棄されている麦芽根の有効利用
を図ることを目的とするものである。As described above, the present invention aims to effectively utilize malt root, which is hardly available at present and a large amount of which is discarded due to the use of malt.
本発明者らは、上記殆ど利用されていない麦芽根の有
効な利用について鋭意研究の結果、麦芽根の水抽出物が
線維芽細胞の増殖を促進する作用を見出し、この物質を
人の皮膚に塗布することにより、皮膚組織の活性化と皮
膚の創傷の治癒に有効である知見を得て本発明を完成し
た。The present inventors, as a result of earnest research on the effective use of the malt root which is rarely used, found that the water extract of malt root promotes the proliferation of fibroblasts, this substance on human skin The present invention has been completed by finding that the application is effective in activating skin tissue and healing skin wounds.
本発明は麦芽根の水抽出物を有効成分とする外用剤で
ある。The present invention is an external preparation containing an aqueous extract of malt root as an active ingredient.
本発明に使用する麦芽根の水抽出物は、水飴，ビール
の製造に用いるために切除された麦芽根を粉砕し、粉末
にしたものを常法により水で抽出したものである。The water extract of malt root used in the present invention is obtained by crushing the malt root excised for use in the production of starch syrup and beer and pulverizing it to extract it with water by a conventional method.
例えば、麦芽根粉末を水中に浸漬し、室温で放置後、
沸騰水で数分間抽出し、濾過して得られた水溶液であ
る。For example, dip malt root powder in water, leave it at room temperature,
An aqueous solution obtained by extracting with boiling water for several minutes and filtering.
本発明の外用剤は上記のようにして得た麦芽根抽出液
を通常の外用剤基剤，助剤などに配合調製してローショ
ン剤，軟膏，乳剤，パップ剤などの外用剤とする。麦芽
根抽出物は各種外用剤の全量に対し0.5〜10％重量程度
配合すれば充分その効果を奏し得るものである。The external preparation of the present invention is prepared by mixing the malt root extract obtained as described above with a usual base for external preparations, auxiliaries and the like to give external preparations such as lotions, ointments, emulsions and poultices. If the malt root extract is blended in an amount of about 0.5 to 10% by weight with respect to the total amount of various external preparations, the effect can be sufficiently exhibited.
本発明の外用剤とする場合は、例えばローション剤に
おいては、精製水にグリセリン，プロピレングリコール
などの保湿剤，皮膚栄養剤などを溶かし、防腐剤，香料
などをアルコールに溶解し、両者を混合して室温下に可
溶化する一般のローション剤の製造において、水相に有
効成分である麦芽根の水抽出液を加えてローション剤と
する。When the external preparation of the present invention is used, for example, in a lotion, a moisturizing agent such as glycerin and propylene glycol, a skin nutrient, etc. are dissolved in purified water, and a preservative, a fragrance, etc. are dissolved in alcohol, and both are mixed. In the production of a general lotion that is solubilized at room temperature, a lotion is prepared by adding a water extract of malt root, which is an active ingredient, to the aqueous phase.
軟膏においては、精製水に親水性成分例えばグリセリ
ン，ソルビットなどの保湿剤を添加して水相部とし、油
相部はミツロウ，パラフィン，マイクロクリスタリンワ
ックス，セレシン，高級脂肪酸，硬化油などの固形油
分、ワセリン，ラノリン，グリセリドなどの半固形油分
それにスクワラン，流動パラフィン，各種エステル油な
どの液状油分に防腐剤，界面活性剤などの油性成分を添
加し調製する。このようにして得られた水相部を加温し
て、緩やかに攪拌しつつ、同温度に加温された油相部を
徐々に添加し乳化して軟膏とする一般の軟膏の製造にお
いて、水相部に有効成分である麦芽根の水抽出液を加え
て軟膏とする。In an ointment, a hydrophilic component such as glycerin or sorbit is added to purified water to form an aqueous phase part, and an oil phase part is a solid oil component such as beeswax, paraffin, microcrystalline wax, ceresin, higher fatty acid, or hardened oil. It is prepared by adding semi-solid oils such as petrolatum, lanolin and glyceride and liquid oils such as squalane, liquid paraffin and various ester oils with oil components such as preservatives and surfactants. The aqueous phase obtained in this way is heated, while gently stirring, in the production of a general ointment to gradually add the oil phase heated to the same temperature to emulsify it into an ointment, An ointment is prepared by adding a water extract of malt root, which is an active ingredient, to the aqueous phase.
乳剤においては、精製水にグリセリンなどの保湿剤、
酸又はアルカリのpH調製剤などを加え、加熱混合してエ
タノールを加え水相部とし、ミツロウ，パラフィンなど
の固形油分、ワセリン，ラノリンなどの半固形油分、ス
クワラン，流動パラフィン，各種エステル油などの液状
油分に、防腐剤，界面活性剤などの油性成分を添加調製
して混合加熱し油相部とし、油相部を水相部に加えて予
備乳化を行い、これにカルボキシビニルポリマー，カル
ボキシメチルセルロースなどの保護コロイド剤を加え、
ホモミキサーで均一に乳化して乳剤とする一般の乳剤の
製造において、水相部に有効成分である麦芽根の水抽出
液を水相部に加えて乳剤とする。In emulsions, humectants such as glycerin in purified water,
Add an acid or alkali pH adjuster, mix by heating and add ethanol to form the aqueous phase. Solid oils such as beeswax and paraffin, semisolid oils such as petrolatum and lanolin, squalane, liquid paraffin, and various ester oils. Oil components such as preservatives and surfactants are added to the liquid oil, mixed and heated to form the oil phase part, and the oil phase part is added to the water phase part for preliminary emulsification, and then carboxyvinyl polymer, carboxymethyl cellulose Add a protective colloid agent such as
In the production of a general emulsion which is uniformly emulsified with a homomixer to give an emulsion, an aqueous extract of malt root, which is an active ingredient, is added to the aqueous phase to form an emulsion.
パップ剤においては、精製水にグリセリンなどの保護
剤、ポリビニルアルコール，ビーガムなどの皮膚剤など
を加えて膨潤させ、これに必要があればカオリン，タル
ク，酸化亜鉛などの粉末を加え、香料，防腐剤などを溶
解したエタノールを加えて、ペースト状となるまで混練
する一般のパップ剤の製造において、有効成分である麦
芽根の水抽出物を加えてパップ剤とする。For poultices, a protective agent such as glycerin or a skin agent such as polyvinyl alcohol or bee gum is added to purified water to swell it, and if necessary, powders such as kaolin, talc, or zinc oxide are added to add fragrance or antiseptic. In the production of a general poultice, which is prepared by adding ethanol in which an agent or the like is dissolved and kneading until a paste is formed, a water extract of malt root, which is an active ingredient, is added to give a poultice.
次に本発明の有効成分である麦芽根水抽出液の製造法
並びに同物質の線維芽細胞増殖作用及び創傷治癒効果を
示す試験例を挙げる。Next, a method for producing an aqueous extract of malt root, which is an active ingredient of the present invention, and test examples showing the fibroblast proliferation action and wound healing effect of the substance will be given.
製造例 上部に被粉砕物投入口、一側に粉砕物排出口を有する
粉砕器本体内にセラミックにより形成された固定盤とこ
れに対峙して固定盤との対向面にダイヤモンドより成る
研削面を有し、その中心軸を駆動器により回転自在に設
置した回転盤より成る粉砕器の固定盤と回転盤の間隙を
約10ミクロンに設定し、同粉砕機の投入口より麦芽根を
投入し、回転盤を回転させて、麦芽根を微粉砕する。Manufacturing example A fixed plate made of ceramics in the main body of the crusher having a crushed material input port on the upper side and a crushed material discharge port on one side, and a grinding surface made of diamond on the surface facing the fixed plate facing the fixed plate. Having a fixed plate and a rotary plate of a crusher consisting of a rotary plate whose center axis is rotatably installed by a driver, set the gap to about 10 microns, and put malt root from the input port of the crusher, Rotate the turntable to finely grind the malt root.
かくして得られた麦芽根粉末1gを10mlの水に、室温で
15分間浸漬した。この液に更に水10mlを加えた後、水を
沸騰させ、15分間維持する。この液を濾紙（NO.131）を
用いて濾過し、濾液25mlを採取した。1 g of malt root powder thus obtained was added to 10 ml of water at room temperature.
Soaked for 15 minutes. After adding another 10 ml of water to this solution, the water is boiled and kept for 15 minutes. This solution was filtered using filter paper (NO. 131), and 25 ml of the filtrate was collected.
試験例１ 線維芽細胞増殖試験 （１） 使用細胞 マウス由来の線維芽細胞（Balb/3TI2−３） （２） 培養基 ５％仔牛血清含有イーグル（Eagle′ｓ）のMEM培地。Test Example 1 Fibroblast proliferation test (1) Cells used Fibroblasts derived from mouse (Balb / 3TI2-3) (2) Culture medium MEM medium containing 5% fetal calf serum (Eagle's).
（３） 試験区 Ａ区： 製造例で得られた麦芽根抽出液を培地に0.2％濃度で加
えた区。(3) Test Group A Group: A group in which the malt root extract obtained in Production Example was added to the medium at a concentration of 0.2%.
Ｂ区： 製造例で得られた麦芽根抽出液を培地に0.4％濃度で加
えた区。Group B: Group in which the malt root extract obtained in Production Example was added to the medium at a concentration of 0.4%.
Ｃ区： 製造例で得られた麦芽根抽出液を培地に0.7％濃度で加
えた区。Group C: A group in which the malt root extract obtained in Production Example was added to the medium at a concentration of 0.7%.
Ｄ区： 製造例で得られた麦芽根抽出液を培地に１％濃度で加え
た区。Group D: Group in which the malt root extract obtained in Production Example was added to the medium at a concentration of 1%.
Ｅ区： 麦芽根抽出液を加えない区。（コントロール） （４） 試験方法 各試験区とも２枚のシャーレ（Falcon 3002）にそれ
ぞれマウス由来の線維芽細胞を４×104個細胞を播種
し、５日間培養を行い、培地交換は３日毎に行った。５
日間培養後、自動血球計数装置（東亜医用電子製）を用
いて、シャーレ当たりの細胞数を測定した。各添加濃度
毎のシャーレについて測定し、２枚の平均値を測定結果
とした。E zone: A zone in which the malt root extract is not added. (Control) (4) Test method In each test section, 4 x 10 4 cells of mouse-derived fibroblasts were seeded on each of two petri dishes (Falcon 3002) and cultured for 5 days, and the medium was replaced every 3 days. Went to. 5
After culturing for a day, the number of cells per petri dish was measured using an automatic blood cell counter (Toa Iyo Denshi). The petri dish for each added concentration was measured, and the average value of the two sheets was used as the measurement result.
（５） 結果 上記試験の結果を表１に示す。(5) Results Table 1 shows the results of the above test.
この結果から明らかな通り、麦芽根抽出液の0.2〜１
％添加において、添加濃度に比例して線維芽細胞の増殖
が認められた。１％添加における線維芽細胞の増殖率は
コントロール比172.5％であっつた。 As is clear from this result, 0.2-1 of malt root extract
%, The proliferation of fibroblasts was observed in proportion to the concentration added. The proliferation rate of fibroblasts at the addition of 1% was 172.5% of the control ratio.
以上の通り、本発明の有効成分である麦芽根の抽出液
には線維芽細胞の増殖作用がある。As described above, the extract of malt root, which is the active ingredient of the present invention, has a proliferation effect on fibroblasts.
試験例２ 創傷治癒効果試験 （１） 試験方法 生後７週令のウイスター系ラットを６匹１群として試
験に用いた。ラット背部を剃毛後、正中線に沿って２〜
３個所皮膚を切開し、ただちに切開部を縫合した。その
後、製造例で得られた麦芽根抽出液及び対照として生理
食塩水を１日１回２週間塗布した。Test Example 2 Wound healing effect test (1) Test method Six Wistar rats aged 7 weeks were used as a group of 6 rats. After shaving the rat's back, 2 along the midline
The skin was incised at three locations, and the incision was immediately sutured. Thereafter, the malt root extract obtained in Production Example and physiological saline as a control were applied once a day for 2 weeks.
２週間後、ラットを屠殺し、切開部を中心に幅2cmの
短冊状の皮膚切片を作製した。この切片はテンシロンUT
M−II−20（TOKO BALDWIN Co.LTD.）を用いて切断強度
を測定した。Two weeks later, the rat was sacrificed, and a strip-shaped skin section with a width of 2 cm centering on the incision was prepared. This section is Tensilon UT
The cutting strength was measured using M-II-20 (TOKO BALDWIN Co.LTD.).
（２） 結果 表２の通りであった。(2) Results The results are shown in Table 2.
この結果より明らかな通り、本発明の有効成分である
麦芽根抽出液塗布部位は対照の生理食塩水塗布部位に比
べ張力が増加し、顕著な創傷治療効果が認められた。 As is clear from these results, the site of application of the malt root extract, which is the active ingredient of the present invention, had an increased tension as compared to the site of application of the control saline solution, and a remarkable wound healing effect was observed.
次に本発明の実施例を示す。 Next, examples of the present invention will be described.
例１ 〈軟膏〉 麦芽根抽出液（製造例で得たもの） 2.0％ ポリオキシエチレンステアリルエーテル 2.0％ ポリオキシエチレンセチルエーテル 2.0％ ミツロウ 6.0％ セタノール 6.0％ イソプロピルパルミテート 10.0％ 流動パラフィン 30.0％ ポリエチレングリコールモノステアレート 1.0％ パラオキシ安息香酸メチル 0.2％ 精製水 全量を100とする 例２ 〈乳剤〉 麦芽根抽出液（製造例で得たもの） 1.0％ 自己乳化型グリセロールモノステアレート 0.5％ ポリオキシエチレンセチルエーテル 2.0％ MCステアリン酸 2.0％ セタノール 1.5％ イソプロピルミリステート 10.0％ パラオキシ安息香酸 0.2％ 香料 微量 精製水 全量を100とする 例３ 〈パップ剤〉 麦芽根抽出液（製造例で得たもの） 5.0％ パラオキシ安息香酸メチル 0.08％ カルボキシビニルポリマー 1.0％ 炭酸カルシウム 0.6％ 二酸化チタン 0.02％ 香料 微量 精製水 全量を100とする 例４ 〈ローション剤〉 麦芽根抽出液（製造例で得たもの） 3.0％ パラオキシ安息香酸 0.08％ クエン酸 0.3％ 香料 微量 精製水 全量を100とする 〔発明の効果〕 本発明は従来殆ど実用性がなかった麦芽根の水抽出液
を線維細胞の増殖作用、皮膚の損傷の治癒に有効な外用
剤として極めて有用な発明である。Example 1 <Ointment> Malt root extract (obtained in production example) 2.0% Polyoxyethylene stearyl ether 2.0% Polyoxyethylene cetyl ether 2.0% Beeswax 6.0% Cetanol 6.0% Isopropyl palmitate 10.0% Liquid paraffin 30.0% Polyethylene glycol Monostearate 1.0% Methyl paraoxybenzoate 0.2% Purified water Total amount 100 Example 2 <Emulsion> Malt root extract (obtained in production example) 1.0% Self-emulsifying glycerol monostearate 0.5% Polyoxyethylene cetyl Ether 2.0% MC Stearic acid 2.0% Cetanol 1.5% Isopropyl myristate 10.0% Paraoxybenzoic acid 0.2% Perfume Trace amount of purified water 100 as total Example 3 <Pap agent> Malt root extract (obtained in production example) 5.0% Methyl paraoxybenzoate 0.08% Carboxyvinyl polymer 1.0% Calcium acid 0.6% Titanium dioxide 0.02% Fragrance Trace amount of purified water Total amount 100 Example 4 <Lotion> Malt root extract (obtained in production example) 3.0% Paraoxybenzoic acid 0.08% Citric acid 0.3% Fragrance Trace purified water The total amount is 100 [Effect of the invention] The present invention is an extremely useful invention as an external preparation effective for treating the damage of skin damage by the aqueous extract of malt root, which has hardly been practically used in the past. .
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ６１Ｋ 9/70 Ａ６１Ｋ 9/70 ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI Technical display area A61K 9/70 A61K 9/70
Claims (1)
ることを特徴とする外用剤。1. An external preparation containing a water extract of malt root as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62137072A JP2531679B2 (en) | 1987-05-30 | 1987-05-30 | Topical |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62137072A JP2531679B2 (en) | 1987-05-30 | 1987-05-30 | Topical |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63301828A JPS63301828A (en) | 1988-12-08 |
| JP2531679B2 true JP2531679B2 (en) | 1996-09-04 |
Family
ID=15190245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62137072A Expired - Lifetime JP2531679B2 (en) | 1987-05-30 | 1987-05-30 | Topical |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2531679B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU689036B2 (en) * | 1995-05-10 | 1998-03-19 | Kureha Chemical Industry Co., Ltd. | Pharmaceutical composition containing substance inhibiting HSP47 production |
| JP2002097149A (en) * | 2000-09-20 | 2002-04-02 | Noevir Co Ltd | Skin care preparation |
| JP2002104952A (en) * | 2000-09-28 | 2002-04-10 | Noevir Co Ltd | Skin case preparation |
| TWI337844B (en) * | 2003-04-11 | 2011-03-01 | Suntory Holdings Ltd | Method for producing food by using wheat germ root |
-
1987
- 1987-05-30 JP JP62137072A patent/JP2531679B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63301828A (en) | 1988-12-08 |
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