JP2518124B2 - Biochemical automatic analyzer - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biochemical automatic analyzer for measuring the concentration or activity value of a target component in a sample containing multiple components such as serum and urine. More specifically, the present invention relates to improvement of a pipette dispensing means used when sampling a liquid sample.

[0002]

2. Description of the Related Art Conventionally, when analyzing a plurality of items of one specimen with an automatic biochemical analyzer, a pipettor and an automatic aspirator are used.
In the first item, [air + excessive amount of sample for preventing thinning + predetermined amount] is sucked, a predetermined amount is discharged in the cell, and in the second and subsequent items, a predetermined amount is sucked and discharged. FIG. 4 is a schematic view of the vicinity of a pipette nozzle of a conventional sample dispensing system. In FIG. 4, 1 is a sample sample, 2 is an excess amount, 3 is air, 4
Is water. In this conventional method, the first item is [Air +
The excess amount for preventing thinning + predetermined amount] is sucked (a), and then a predetermined amount is discharged into the cell (b). The second item is sucking a predetermined amount (c) and then discharging a predetermined amount into the cell (d). After the third item, the same operation as the second item is performed.

[0003]

However, in the above-mentioned prior art, when the number of inspection items increases, the influence of dizziness appears, resulting in a difference in the sample amount between items, resulting in a decrease in accuracy and precision.

In order to solve the above-mentioned problems of the prior art, it is an object of the present invention to improve the accuracy / accuracy in aspirating and dispensing a pipette used in sampling a liquid sample.

[0005]

In order to achieve the above object, the biochemical automatic analyzer according to the present invention has a pipetting means for pipetting a suction for preventing thinning of each item when sampling a liquid sample. It is characterized in that it is provided with a means for discharging a part of the sample thus sampled into a sample sample container, re-sucking the sample sample in the container as an excessive amount, and further sucking a predetermined amount and discharging it to the cell.

[0006]

According to the structure of the present invention, the second and subsequent items are
Dispense a part of the excess amount for dizziness prevention into the sample in the sample container, and again aspirate the sample in the container (that is, replace a part of the excess amount) and place it in the same condition as in the first item. By sucking / discharging a fixed amount, a part of the sample for preventing dizziness is replaced, so that the influence of dizziness on a predetermined amount is reduced, and the difference in the amount of sample between items is reduced. As a result,
Accuracy is improved.

[0007]

EXAMPLE FIG. 1 shows a schematic diagram of a sample dispensing system according to an example of the present invention. In the first item, [air + excess amount for preventing thinning + predetermined amount] is sucked (A), and then a predetermined amount is discharged to the cell (B). This is the same as the conventional method, but the second item is to discharge a part of the total amount in the sample container (C), re-suck the same amount as the next discharged amount (D), and then Aspirate a predetermined amount (E). Finally, a predetermined amount is discharged into the cell (F). The third and subsequent items are operated in the same manner as the second item. Note that any known automatic device may be used for the dispensing itself. For example, using a pipettor and a known automatic suction device.

2 and 3 are graphs showing the difference between the items of the method of the present invention (marked by ◯) and the method of the prior art (marked by □) (error (%) from the first item when the dispensing amount is constant). . Figure 2 shows 5
FIG. 3 shows the case where the dispensed amount is 30 μm. In addition, FIGS. 2 to 3 show the average of 20 samples in the case of 10 items per sample. The sample used was an orange G dye dissolved in a commercially available pooled serum. In the conventional dispensing method, as the number of items increases, 0. Although the influence of thinning is several percent, it was confirmed that the dispensing method of the present invention reduces the influence of thinning as compared with the conventional method.

As is clear from FIGS. 2 and 3, since a part of this extra amount is about several μl, even if the number of measurement items increases, the thinning of the whole sample in the sample container has almost no effect. There wasn't.

[0010]

As described above, according to the present invention, at the time of sampling a liquid sample, a part of the sample aspirated for preventing dizziness for each item is discharged to a sample sample container, and the sample in the container is discharged. By re-aspirating the sample as an extra amount, further aspirating a predetermined amount, and ejecting to the cell, even if the number of measurement items increases, the influence of thinning disappears,
The difference in the amount of sample between items becomes small. In addition, the precision and accuracy of pipetting with the pipetter is improved.

[Brief description of drawings]

FIG. 1 shows a schematic diagram of a sample dispensing system according to an embodiment of the present invention.

FIG. 2 is a method according to the present invention (marked with a circle) and a method according to a conventional technique (□
Graph of difference between items (marked) (dispensing amount 5 μm).

FIG. 3 is a method according to the present invention (marked with a circle) and a method according to a conventional technology (□
Graph of difference between items (marked) (dispensing amount 30 μm).

FIG. 4 is a schematic view of the vicinity of a pipette nozzle of a conventional sample dispensing system.

[Explanation of symbols]

 1 Sample Sample 2 Extra amount 3 Air 4 Water

Claims (1)

(57) [Claims] 1. The pipetting device of the pipettor discharges a part of a sample aspirated to prevent thinning into a sample sample container for each item when sampling a liquid sample, and an excess amount of the sample sample in the container is discharged. As a result, the biochemical automatic analyzer is provided with a means for re-aspirating, further aspirating a predetermined amount, and ejecting to a cell.