JP2026501022A - ガラクトース－１－リン酸ウリジルトランスフェラーゼ変異体及びそのｌ－リジンの製造における使用 - Google Patents

ガラクトース－１－リン酸ウリジルトランスフェラーゼ変異体及びそのｌ－リジンの製造における使用

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Publication number
JP2026501022A
JP2026501022A JP2025538710A JP2025538710A JP2026501022A JP 2026501022 A JP2026501022 A JP 2026501022A JP 2025538710 A JP2025538710 A JP 2025538710A JP 2025538710 A JP2025538710 A JP 2025538710A JP 2026501022 A JP2026501022 A JP 2026501022A
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Japan
Prior art keywords
seq
protein
lysine
ncgl2002
recombinant
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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JP2025538710A
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English (en)
Japanese (ja)
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JP2026501022A5 (enExample
Inventor
周暁群
孟剛
魏愛英
趙春光
馬風勇
張英
Original Assignee
寧夏伊品生物科技股▲ふん▼有限公司
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Publication of JP2026501022A publication Critical patent/JP2026501022A/ja
Publication of JP2026501022A5 publication Critical patent/JP2026501022A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0701—UTP--hexose-1-phosphate uridylyltransferase (2.7.7.10)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07012—UDP-glucose--hexose-1-phosphate uridylyltransferase (2.7.7.12)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2025538710A 2022-12-29 2023-03-30 ガラクトース－１－リン酸ウリジルトランスフェラーゼ変異体及びそのｌ－リジンの製造における使用 Pending JP2026501022A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202211706015.0A CN115820706B (zh) 2022-12-29 2022-12-29 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用
CN202211706015.0 2022-12-29
PCT/CN2023/084971 WO2024138928A1 (zh) 2022-12-29 2023-03-30 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Publications (2)

Publication Number Publication Date
JP2026501022A true JP2026501022A (ja) 2026-01-13
JP2026501022A5 JP2026501022A5 (enExample) 2026-04-02

Family

ID=85519246

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2025538710A Pending JP2026501022A (ja) 2022-12-29 2023-03-30 ガラクトース－１－リン酸ウリジルトランスフェラーゼ変異体及びそのｌ－リジンの製造における使用

Country Status (5)

Country Link
US (1) US20260218255A1 (enExample)
EP (1) EP4644555A1 (enExample)
JP (1) JP2026501022A (enExample)
CN (1) CN115820706B (enExample)
WO (1) WO2024138928A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115820706B (zh) * 2022-12-29 2025-07-29 宁夏伊品生物科技股份有限公司 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100789271B1 (ko) * 2005-11-30 2008-01-02 씨제이 주식회사 Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 ｌ-라이신을 생산하는 방법
KR100838038B1 (ko) * 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100838035B1 (ko) * 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
CN105950524A (zh) * 2016-04-27 2016-09-21 齐鲁工业大学 一种高产l-赖氨酸的谷氨酸棒状杆菌工程菌的构建方法
CN110869504A (zh) * 2017-06-07 2020-03-06 齐默尔根公司 来自谷氨酸棒状杆菌的启动子及其在调节辅助基因表达中的用途
CN114605509B (zh) * 2022-03-14 2024-06-04 宁夏伊品生物科技股份有限公司 Yh66_01475蛋白及其编码基因在调控细菌精氨酸产量中的应用
CN114835783B (zh) * 2022-06-01 2024-06-25 宁夏伊品生物科技股份有限公司 NCgl2747基因突变体及其在制备L-赖氨酸中的应用
CN115820706B (zh) * 2022-12-29 2025-07-29 宁夏伊品生物科技股份有限公司 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Also Published As

Publication number Publication date
WO2024138928A1 (zh) 2024-07-04
CN115820706B (zh) 2025-07-29
US20260218255A1 (en) 2026-07-30
EP4644555A1 (en) 2025-11-05
CN115820706A (zh) 2023-03-21

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