JP2025017371A5 - - Google Patents
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- JP2025017371A5 JP2025017371A5 JP2024191126A JP2024191126A JP2025017371A5 JP 2025017371 A5 JP2025017371 A5 JP 2025017371A5 JP 2024191126 A JP2024191126 A JP 2024191126A JP 2024191126 A JP2024191126 A JP 2024191126A JP 2025017371 A5 JP2025017371 A5 JP 2025017371A5
- Authority
- JP
- Japan
- Prior art keywords
- culture
- conditionally immortalized
- microvascular endothelial
- human conditionally
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000003668 pericyte Anatomy 0.000 claims 34
- 210000004556 brain Anatomy 0.000 claims 27
- 210000004925 microvascular endothelial cell Anatomy 0.000 claims 27
- 230000004069 differentiation Effects 0.000 claims 25
- 210000001130 astrocyte Anatomy 0.000 claims 23
- 238000000034 method Methods 0.000 claims 17
- 239000002609 medium Substances 0.000 claims 14
- 239000001963 growth medium Substances 0.000 claims 12
- 238000004113 cell culture Methods 0.000 claims 9
- 230000008499 blood brain barrier function Effects 0.000 claims 8
- 210000001218 blood-brain barrier Anatomy 0.000 claims 8
- 238000012258 culturing Methods 0.000 claims 6
- 238000010899 nucleation Methods 0.000 claims 5
- 239000000758 substrate Substances 0.000 claims 5
- 239000010410 layer Substances 0.000 claims 4
- 239000003102 growth factor Substances 0.000 claims 2
- 239000002356 single layer Substances 0.000 claims 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 1
- 210000002744 extracellular matrix Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000002569 neuron Anatomy 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024191126A JP2025017371A (ja) | 2020-04-01 | 2024-10-30 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020065670A JP7650480B2 (ja) | 2020-04-01 | 2020-04-01 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
| JP2024191126A JP2025017371A (ja) | 2020-04-01 | 2024-10-30 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020065670A Division JP7650480B2 (ja) | 2020-04-01 | 2020-04-01 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2025017371A JP2025017371A (ja) | 2025-02-05 |
| JP2025017371A5 true JP2025017371A5 (enExample) | 2025-04-04 |
Family
ID=78001522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020065670A Active JP7650480B2 (ja) | 2020-04-01 | 2020-04-01 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
| JP2024191126A Pending JP2025017371A (ja) | 2020-04-01 | 2024-10-30 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020065670A Active JP7650480B2 (ja) | 2020-04-01 | 2020-04-01 | ヒト条件的不死化細胞を用いた血液脳関門モデルおよびその製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (2) | JP7650480B2 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115354016A (zh) * | 2022-09-13 | 2022-11-18 | 中山大学 | 一种体外血脑屏障模型的构建方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11447752B2 (en) * | 2016-04-15 | 2022-09-20 | Yamaguchi University | In vitro model for blood-brain barrier and method for producing in vitro model for blood-brain barrier |
-
2020
- 2020-04-01 JP JP2020065670A patent/JP7650480B2/ja active Active
-
2024
- 2024-10-30 JP JP2024191126A patent/JP2025017371A/ja active Pending
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