JP2024533335A - オリゴヌクレオチド二本鎖におけるセンス鎖及びアンチセンス鎖を検出するための方法 - Google Patents

オリゴヌクレオチド二本鎖におけるセンス鎖及びアンチセンス鎖を検出するための方法 Download PDF

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JP2024533335A
JP2024533335A JP2024515136A JP2024515136A JP2024533335A JP 2024533335 A JP2024533335 A JP 2024533335A JP 2024515136 A JP2024515136 A JP 2024515136A JP 2024515136 A JP2024515136 A JP 2024515136A JP 2024533335 A JP2024533335 A JP 2024533335A
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sense
antisense
probe
oligonucleotide
strand
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JP2024533335A5 (cg-RX-API-DMAC10.html
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ビー． ハーキンズ，セス
ジェー． ブレイク，ティモシー
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メソ スケール テクノロジーズ エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2024515136A 2021-09-09 2022-09-08 オリゴヌクレオチド二本鎖におけるセンス鎖及びアンチセンス鎖を検出するための方法 Pending JP2024533335A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163242208P 2021-09-09 2021-09-09
US63/242,208 2021-09-09
PCT/US2022/076091 WO2023039459A1 (en) 2021-09-09 2022-09-08 Method for detecting sense and antisense strands in an oligonucleotide duplex

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JP2024533335A true JP2024533335A (ja) 2024-09-12
JP2024533335A5 JP2024533335A5 (cg-RX-API-DMAC10.html) 2025-09-17

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US (1) US20230141860A1 (cg-RX-API-DMAC10.html)
EP (2) EP4596715A3 (cg-RX-API-DMAC10.html)
JP (1) JP2024533335A (cg-RX-API-DMAC10.html)
KR (1) KR20240070569A (cg-RX-API-DMAC10.html)
CN (1) CN118234872A (cg-RX-API-DMAC10.html)
AU (1) AU2022341113A1 (cg-RX-API-DMAC10.html)
CA (1) CA3231524A1 (cg-RX-API-DMAC10.html)
DK (1) DK4399331T3 (cg-RX-API-DMAC10.html)
FI (1) FI4399331T3 (cg-RX-API-DMAC10.html)
IL (1) IL311348A (cg-RX-API-DMAC10.html)
TW (1) TW202317150A (cg-RX-API-DMAC10.html)
WO (1) WO2023039459A1 (cg-RX-API-DMAC10.html)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250243529A1 (en) * 2024-01-25 2025-07-31 Meso Scale Technologies, Llc. Methods for multiplex detection of sense and antisense strands in an oligonucleotide duplex
WO2026039455A1 (en) 2024-08-13 2026-02-19 Meso Scale Technologies, Llc. Detection of nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673533B1 (en) 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
JP2004534226A (ja) 2001-06-29 2004-11-11 メソ スケイル テクノロジーズ，エルエルシー 発光試験測定用のアッセイプレート、リーダシステム及び方法
WO2005021800A2 (en) * 2003-08-22 2005-03-10 Sirna Therapeutics, Inc. Detection and quantitation of nucleic acid molecules in biological samples
EP1963853B1 (en) 2005-12-21 2016-03-09 Meso Scale Technologies, LLC Assay modules having assay reagents and methods of making and using same
CA3177188A1 (en) 2011-01-06 2012-07-12 Meso Scale Technologies, Llc. Assay cartridges for pcr analysis and methods of use thereof
AU2020268813B2 (en) * 2019-05-03 2026-06-25 Meso Scale Technologies, Llc. Kits for detecting one or more target nucleic acid analytes in a sample and methods of making and using the same

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Publication number Publication date
EP4399331B1 (en) 2025-05-28
EP4596715A3 (en) 2025-10-29
EP4596715A2 (en) 2025-08-06
US20230141860A1 (en) 2023-05-11
AU2022341113A1 (en) 2024-03-21
IL311348A (en) 2024-05-01
TW202317150A (zh) 2023-05-01
DK4399331T3 (da) 2025-08-25
EP4399331A1 (en) 2024-07-17
FI4399331T3 (fi) 2025-08-22
CA3231524A1 (en) 2023-03-16
KR20240070569A (ko) 2024-05-21
CN118234872A (zh) 2024-06-21
WO2023039459A1 (en) 2023-03-16

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