DK4399331T3 - Fremgangsmåde til detektering af sense- og antisense-strenge i et oligonukleotiddupleks - Google Patents

Fremgangsmåde til detektering af sense- og antisense-strenge i et oligonukleotiddupleks

Info

Publication number
DK4399331T3
DK4399331T3 DK22786684.5T DK22786684T DK4399331T3 DK 4399331 T3 DK4399331 T3 DK 4399331T3 DK 22786684 T DK22786684 T DK 22786684T DK 4399331 T3 DK4399331 T3 DK 4399331T3
Authority
DK
Denmark
Prior art keywords
antisense strands
oligonucleotide duplex
detecting sense
sense
detecting
Prior art date
Application number
DK22786684.5T
Other languages
English (en)
Inventor
Seth B Harkins
Timothy J Break
Original Assignee
Meso Scale Technologies Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meso Scale Technologies Llc filed Critical Meso Scale Technologies Llc
Application granted granted Critical
Publication of DK4399331T3 publication Critical patent/DK4399331T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK22786684.5T 2021-09-09 2022-09-08 Fremgangsmåde til detektering af sense- og antisense-strenge i et oligonukleotiddupleks DK4399331T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163242208P 2021-09-09 2021-09-09
PCT/US2022/076091 WO2023039459A1 (en) 2021-09-09 2022-09-08 Method for detecting sense and antisense strands in an oligonucleotide duplex

Publications (1)

Publication Number Publication Date
DK4399331T3 true DK4399331T3 (da) 2025-08-25

Family

ID=83688678

Family Applications (1)

Application Number Title Priority Date Filing Date
DK22786684.5T DK4399331T3 (da) 2021-09-09 2022-09-08 Fremgangsmåde til detektering af sense- og antisense-strenge i et oligonukleotiddupleks

Country Status (12)

Country Link
US (1) US20230141860A1 (da)
EP (2) EP4399331B1 (da)
JP (1) JP2024533335A (da)
KR (1) KR20240070569A (da)
CN (1) CN118234872A (da)
AU (1) AU2022341113A1 (da)
CA (1) CA3231524A1 (da)
DK (1) DK4399331T3 (da)
FI (1) FI4399331T3 (da)
IL (1) IL311348A (da)
TW (1) TW202317150A (da)
WO (1) WO2023039459A1 (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250243529A1 (en) * 2024-01-25 2025-07-31 Meso Scale Technologies, Llc. Methods for multiplex detection of sense and antisense strands in an oligonucleotide duplex

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673533B1 (en) 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
DK2461156T3 (da) 2001-06-29 2020-08-03 Meso Scale Technologies Llc Indretning til luminescenstestmålinger
US7521184B2 (en) * 2003-08-22 2009-04-21 Sirna Therapeutics, Inc. Detection and quantitation of nucleic acid molecules in biological samples
AU2006330913B2 (en) 2005-12-21 2011-10-27 Meso Scale Technologies, Llc Assay modules having assay reagents and methods of making and using same
JP6087293B2 (ja) 2011-01-06 2017-03-01 メソ スケール テクノロジーズ エルエルシー アッセイカートリッジ及びその使用方法
EP3963100A1 (en) 2019-05-03 2022-03-09 Meso Scale Technologies, LLC Kits for detecting one or more target nucleic acid analytes in a sample and methods of making and using the same

Also Published As

Publication number Publication date
FI4399331T3 (fi) 2025-08-22
EP4399331B1 (en) 2025-05-28
CA3231524A1 (en) 2023-03-16
EP4596715A3 (en) 2025-10-29
CN118234872A (zh) 2024-06-21
IL311348A (en) 2024-05-01
EP4399331A1 (en) 2024-07-17
WO2023039459A1 (en) 2023-03-16
TW202317150A (zh) 2023-05-01
KR20240070569A (ko) 2024-05-21
EP4596715A2 (en) 2025-08-06
US20230141860A1 (en) 2023-05-11
AU2022341113A1 (en) 2024-03-21
JP2024533335A (ja) 2024-09-12

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