JP2024531957A - ｉｎ ｓｉｔｕエピトランスクリプトームプロファイル - Google Patents

ｉｎ ｓｉｔｕエピトランスクリプトームプロファイル Download PDF

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Publication number
JP2024531957A
JP2024531957A JP2024508669A JP2024508669A JP2024531957A JP 2024531957 A JP2024531957 A JP 2024531957A JP 2024508669 A JP2024508669 A JP 2024508669A JP 2024508669 A JP2024508669 A JP 2024508669A JP 2024531957 A JP2024531957 A JP 2024531957A
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Japan
Prior art keywords
probe
rna
complementary
oligonucleotide
probes
Prior art date
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Pending
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JP2024508669A
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English (en)
Japanese (ja)
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JP2024531957A5 (cg-RX-API-DMAC7.html
Inventor
ワン，シャオ
ゼン，フー
レン，ジンイー
ティアン，ジアクン
グォ，ジァンティン
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Broad Institute
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Broad Institute
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Publication of JP2024531957A publication Critical patent/JP2024531957A/ja
Publication of JP2024531957A5 publication Critical patent/JP2024531957A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2024508669A 2021-08-10 2022-08-10 ｉｎ ｓｉｔｕエピトランスクリプトームプロファイル Pending JP2024531957A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163231585P 2021-08-10 2021-08-10
US63/231,585 2021-08-10
PCT/US2022/039895 WO2023018756A1 (en) 2021-08-10 2022-08-10 In situ epitranscriptomic profiling

Publications (2)

Publication Number Publication Date
JP2024531957A true JP2024531957A (ja) 2024-09-03
JP2024531957A5 JP2024531957A5 (cg-RX-API-DMAC7.html) 2025-08-20

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JP2024508669A Pending JP2024531957A (ja) 2021-08-10 2022-08-10 ｉｎ ｓｉｔｕエピトランスクリプトームプロファイル

Country Status (7)

Country Link
EP (1) EP4384635A1 (cg-RX-API-DMAC7.html)
JP (1) JP2024531957A (cg-RX-API-DMAC7.html)
KR (1) KR20240046547A (cg-RX-API-DMAC7.html)
CN (1) CN118076749A (cg-RX-API-DMAC7.html)
AU (1) AU2022326458A1 (cg-RX-API-DMAC7.html)
CA (1) CA3228406A1 (cg-RX-API-DMAC7.html)
WO (1) WO2023018756A1 (cg-RX-API-DMAC7.html)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112243463B (zh) 2018-04-09 2024-12-13 小利兰·斯坦福大学托管委员会 原位基因测序方法
US12674196B2 (en) 2022-01-18 2026-07-07 10X Genomics, Inc. Methods and compositions for improved probe specificity
WO2025059443A1 (en) 2023-09-15 2025-03-20 The Broad Institute, Inc. Untargeted multiplexed in situ rna profiling and the uses and means therefor
WO2025136916A1 (en) 2023-12-18 2025-06-26 The Board Institute, Inc. Thick tissue transcriptomics and translatomics

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US20170211133A1 (en) * 2011-05-11 2017-07-27 Olink Bioscience Ab Unfolding Proximity Probes and Methods for the Use Thereof
JP2018514230A (ja) * 2015-04-24 2018-06-07 アティラ バイオシステムズ， インクＡｔｉｌａ Ｂｉｏｓｙｓｔｅｍｓ， Ｉｎｃ 限られたヌクレオチド組成を有するプライマーを用いた増幅
WO2019199579A1 (en) * 2018-04-09 2019-10-17 The Board Of Trustees Of The Leland Stanford Junior University Method of in situ gene sequencing
US20210072143A1 (en) * 2019-07-01 2021-03-11 Davinci Bio, Inc. High capacity molecule detection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017053762A1 (en) * 2015-09-24 2017-03-30 Sigma-Aldrich Co. Llc Methods and reagents for molecular proximity detection using rna-guided nucleic acid binding proteins
US12110548B2 (en) * 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US20170211133A1 (en) * 2011-05-11 2017-07-27 Olink Bioscience Ab Unfolding Proximity Probes and Methods for the Use Thereof
JP2018514230A (ja) * 2015-04-24 2018-06-07 アティラ バイオシステムズ， インクＡｔｉｌａ Ｂｉｏｓｙｓｔｅｍｓ， Ｉｎｃ 限られたヌクレオチド組成を有するプライマーを用いた増幅
WO2019199579A1 (en) * 2018-04-09 2019-10-17 The Board Of Trustees Of The Leland Stanford Junior University Method of in situ gene sequencing
US20210072143A1 (en) * 2019-07-01 2021-03-11 Davinci Bio, Inc. High capacity molecule detection

Also Published As

Publication number Publication date
KR20240046547A (ko) 2024-04-09
AU2022326458A1 (en) 2024-02-22
EP4384635A1 (en) 2024-06-19
CN118076749A (zh) 2024-05-24
WO2023018756A1 (en) 2023-02-16
CA3228406A1 (en) 2023-02-16

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