JP2024517998A - 核酸のホスホラミダイトフリー酵素合成のための組成物及び方法 - Google Patents

核酸のホスホラミダイトフリー酵素合成のための組成物及び方法 Download PDF

Info

Publication number
JP2024517998A
JP2024517998A JP2024508996A JP2024508996A JP2024517998A JP 2024517998 A JP2024517998 A JP 2024517998A JP 2024508996 A JP2024508996 A JP 2024508996A JP 2024508996 A JP2024508996 A JP 2024508996A JP 2024517998 A JP2024517998 A JP 2024517998A
Authority
JP
Japan
Prior art keywords
sequence
iisre
adamer
nucleic acid
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2024508996A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024517998A5 (https=
Inventor
ステンプル デレク
マンコウスカ シルビア
ベル ニール
Original Assignee
カメナ バイオサイエンス リミティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by カメナ バイオサイエンス リミティド filed Critical カメナ バイオサイエンス リミティド
Publication of JP2024517998A publication Critical patent/JP2024517998A/ja
Publication of JP2024517998A5 publication Critical patent/JP2024517998A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Luminescent Compositions (AREA)
  • Saccharide Compounds (AREA)
JP2024508996A 2021-04-26 2022-04-26 核酸のホスホラミダイトフリー酵素合成のための組成物及び方法 Pending JP2024517998A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163179828P 2021-04-26 2021-04-26
US63/179,828 2021-04-26
PCT/US2022/026333 WO2022232134A1 (en) 2021-04-26 2022-04-26 Compositions and methods for phosphoramedite-free enzymatic synthesis of nucleic acids

Publications (2)

Publication Number Publication Date
JP2024517998A true JP2024517998A (ja) 2024-04-23
JP2024517998A5 JP2024517998A5 (https=) 2025-05-08

Family

ID=81854492

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024508996A Pending JP2024517998A (ja) 2021-04-26 2022-04-26 核酸のホスホラミダイトフリー酵素合成のための組成物及び方法

Country Status (8)

Country Link
US (1) US20240209407A1 (https=)
EP (1) EP4330388A1 (https=)
JP (1) JP2024517998A (https=)
KR (1) KR20240004602A (https=)
CN (1) CN117597441A (https=)
AU (1) AU2022267235A1 (https=)
CA (1) CA3216430A1 (https=)
WO (1) WO2022232134A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025120370A1 (en) * 2023-12-08 2025-06-12 Camena Bioscience Limited Compositions and methods for solution-phase, phosphoramidite-free synthesis of nucleic acids

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061078A2 (en) * 2001-01-29 2002-08-08 Isis Innovation Limited Streptavidin aptamere
JP2003501069A (ja) * 1999-06-07 2003-01-14 スローニング・ビオテヒノロギー・ゲーエムベーハー Ｄｎａ断片の合成法
US20060194202A1 (en) * 2002-10-18 2006-08-31 Octavian Schatz Method for the manufacture of nucleic acid molecules
JP2009240310A (ja) * 2008-03-31 2009-10-22 Sloning Biotechnology Gmbh 核酸ライブラリーを作製するための方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2909073T3 (es) 2016-09-21 2022-05-05 Univ Muenchen Tech Producción biotecnológica escalable de moléculas de adn monocatenario de secuencia y longitud definidas
US11560565B2 (en) * 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003501069A (ja) * 1999-06-07 2003-01-14 スローニング・ビオテヒノロギー・ゲーエムベーハー Ｄｎａ断片の合成法
WO2002061078A2 (en) * 2001-01-29 2002-08-08 Isis Innovation Limited Streptavidin aptamere
US20060194202A1 (en) * 2002-10-18 2006-08-31 Octavian Schatz Method for the manufacture of nucleic acid molecules
JP2009240310A (ja) * 2008-03-31 2009-10-22 Sloning Biotechnology Gmbh 核酸ライブラリーを作製するための方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TAHIRI-ALAOUI A. ET AL.: "High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands", NUCL ACID RES, vol. 30, no. 10, JPN6026010845, 2002, pages 45, ISSN: 0005820045 *
VAN DEN BRULLE J. ET AL.: "A Novel Solid Phase Technology for High- Throughput Gene Synthesis", BIOTECHNIQUES, vol. 45, no. 3, JPN6026010844, 2008, pages 340 - 343, ISSN: 0005820044 *

Also Published As

Publication number Publication date
EP4330388A1 (en) 2024-03-06
WO2022232134A1 (en) 2022-11-03
AU2022267235A9 (en) 2023-11-16
AU2022267235A1 (en) 2023-11-09
US20240209407A1 (en) 2024-06-27
CA3216430A1 (en) 2022-11-03
KR20240004602A (ko) 2024-01-11
CN117597441A (zh) 2024-02-23

Similar Documents

Publication Publication Date Title
EP4081652B1 (en) Controlled strand-displacement for paired-end sequencing
EP3559267A1 (en) Reagents and methods for molecular barcoding of nucleic acids of single cells
Szostak et al. In vitro selection of functional RNA sequences
US20230017673A1 (en) Methods and Reagents for Molecular Barcoding
EP4347872B1 (en) Oligo-modified nucleotide analogues for nucleic acid preparation
JP2024517998A (ja) 核酸のホスホラミダイトフリー酵素合成のための組成物及び方法
JP2025078774A (ja) 連結された標的捕捉
JP2024520927A (ja) オリゴヌクレオチドプールからの遺伝子アセンブリー
WO2025083338A1 (en) An in vitro method for fragmenting polynucleotides for next generation sequencing
WO2025021984A1 (en) Method for generating a plurality of polynucleotides
JP5128031B2 (ja) ＲｅｃＡタンパク質を用いて標識が導入された核酸を製造する方法
HK40122432A (en) Controlled strand-displacement for paired-end sequencing
CN121065310A (zh) 用于在单细胞实验中对分子加条形码的方法
CN119546761A (zh) 大小受控的核酸片段的制备
CN118019858A (zh) 用于核酸测序的预文库靶标富集
WO2018138218A1 (en) Gene synthesis using templated chemical ligation
HK1240286B (zh) 一种核酸的双接头单链环状文库的构建方法和试剂

Legal Events

Date Code Title Description
RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20250206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20250206

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20250214

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250424

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250424

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20260313

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260318