JP2024513967A - 非ウイルス相同性媒介末端結合 - Google Patents

非ウイルス相同性媒介末端結合 Download PDF

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JP2024513967A
JP2024513967A JP2023562658A JP2023562658A JP2024513967A JP 2024513967 A JP2024513967 A JP 2024513967A JP 2023562658 A JP2023562658 A JP 2023562658A JP 2023562658 A JP2023562658 A JP 2023562658A JP 2024513967 A JP2024513967 A JP 2024513967A
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cdl
target sequence
composition
cell
template
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JP2024513967A5 (enExample
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アーロン クーパー
ペイ－ケン スー
ロバート ムート
アニー トロン
トーマス ガードナー
ブライアン スー
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アーセナル バイオサイエンシズ インコーポレイテッド
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2510/00—Genetically modified cells
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    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2023562658A 2021-04-13 2022-04-13 非ウイルス相同性媒介末端結合 Pending JP2024513967A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163174468P 2021-04-13 2021-04-13
US63/174,468 2021-04-13
PCT/US2022/024703 WO2022221467A1 (en) 2021-04-13 2022-04-13 Non-viral homology mediated end joining

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JP2024513967A true JP2024513967A (ja) 2024-03-27
JP2024513967A5 JP2024513967A5 (enExample) 2025-04-21

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US (1) US20240240164A1 (enExample)
EP (1) EP4323514A4 (enExample)
JP (1) JP2024513967A (enExample)
KR (1) KR20230169221A (enExample)
CN (1) CN117355607A (enExample)
AU (1) AU2022257004A1 (enExample)
BR (1) BR112023021283A2 (enExample)
CA (1) CA3215080A1 (enExample)
CL (2) CL2023003030A1 (enExample)
CO (1) CO2023015207A2 (enExample)
IL (1) IL307447A (enExample)
MX (1) MX2023012044A (enExample)
PE (1) PE20231922A1 (enExample)
WO (1) WO2022221467A1 (enExample)

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TW202442689A (zh) 2023-03-03 2024-11-01 美商亞森諾生物科學公司 靶向ｐｓｍａ及ｃａ９之系統

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020123871A1 (en) * 2018-12-12 2020-06-18 The Regents Of The University Of California Compositions and methods for modifying a target nucleic acid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170000743A1 (en) * 2015-07-02 2017-01-05 Vindico NanoBio Technology Inc. Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles
AU2020301236A1 (en) * 2019-06-25 2021-11-25 Inari Agriculture Technology, Inc. Improved homology dependent repair genome editing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020123871A1 (en) * 2018-12-12 2020-06-18 The Regents Of The University Of California Compositions and methods for modifying a target nucleic acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GENOME BIOLOGY, vol. 18, no. 35, JPN6026001540, 2017, pages 1 - 18, ISSN: 0005865511 *
HUMAN GENE THERAPY, vol. 30, no. 7, JPN6025040640, 2019, pages 814 - 828, ISSN: 0005865514 *
NATURE BIOTECHNOLOGY, vol. 38, JPN6026001542, 2020, pages 44 - 49, ISSN: 0005865512 *
NATURE, vol. 559, JPN6026001541, 2018, pages 405 - 409, ISSN: 0005865513 *

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KR20230169221A (ko) 2023-12-15
CA3215080A1 (en) 2022-10-20
CL2023003030A1 (es) 2024-03-22
IL307447A (en) 2023-12-01
EP4323514A1 (en) 2024-02-21
CO2023015207A2 (es) 2024-02-05
EP4323514A4 (en) 2025-06-04
MX2023012044A (es) 2024-01-05
AU2022257004A1 (en) 2023-11-23
CN117355607A (zh) 2024-01-05
CL2025001317A1 (es) 2025-08-22
US20240240164A1 (en) 2024-07-18
PE20231922A1 (es) 2023-11-28
BR112023021283A2 (pt) 2023-12-19
WO2022221467A1 (en) 2022-10-20

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