JP2024505508A - ポリヌクレオチドによるフローセルの播種効率を改善するための方法、組成物、及びキット - Google Patents

ポリヌクレオチドによるフローセルの播種効率を改善するための方法、組成物、及びキット Download PDF

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JP2024505508A
JP2024505508A JP2023545752A JP2023545752A JP2024505508A JP 2024505508 A JP2024505508 A JP 2024505508A JP 2023545752 A JP2023545752 A JP 2023545752A JP 2023545752 A JP2023545752 A JP 2023545752A JP 2024505508 A JP2024505508 A JP 2024505508A
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flow cell
primer
sequence
bead
beads
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ウー，イア－シュアン
ゴルペ－ヤサル，フィリズ
クマン クラナ，タルン
マーク ブーテル，ジョナサン
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イルミナ インコーポレイテッド
イルミナ ケンブリッジ リミテッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/113—Denaturating agents
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2023545752A 2021-01-29 2022-01-28 ポリヌクレオチドによるフローセルの播種効率を改善するための方法、組成物、及びキット Pending JP2024505508A (ja)

Applications Claiming Priority (3)

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US202163143680P 2021-01-29 2021-01-29
US63/143,680 2021-01-29
PCT/US2022/014329 WO2022165188A1 (en) 2021-01-29 2022-01-28 Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides

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JP2024505508A true JP2024505508A (ja) 2024-02-06
JP2024505508A5 JP2024505508A5 (https=) 2025-02-04

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US (1) US20240117416A1 (https=)
EP (1) EP4284944A1 (https=)
JP (1) JP2024505508A (https=)
KR (1) KR20230154303A (https=)
CN (1) CN117043352A (https=)
AU (1) AU2022212112A1 (https=)
CA (1) CA3206511A1 (https=)
WO (1) WO2022165188A1 (https=)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
WO2023023638A1 (en) * 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Chemical and thermal assisted nucleic acid amplification methods
EP4634400A1 (en) * 2022-12-14 2025-10-22 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates

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JP2016508715A (ja) * 2013-01-09 2016-03-24 イルミナ ケンブリッジ リミテッド 固形支持体でのサンプル調製
WO2019028407A1 (en) * 2017-08-04 2019-02-07 Rocket Lab Usa, Inc. SATELLITE DEPLOYMENT DEVICE DOOR HAVING CLUTCH BEARING
WO2020144373A1 (en) * 2019-01-11 2020-07-16 Illumina Cambridge Limited Complex surface-bound transposome complexes

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CA3206511A1 (en) 2022-08-04
CN117043352A (zh) 2023-11-10
KR20230154303A (ko) 2023-11-07
AU2022212112A1 (en) 2023-08-17
US20240117416A1 (en) 2024-04-11
EP4284944A1 (en) 2023-12-06
WO2022165188A1 (en) 2022-08-04
AU2022212112A9 (en) 2024-10-17

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