CA3206511A1 - Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides - Google Patents
Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides Download PDFInfo
- Publication number
- CA3206511A1 CA3206511A1 CA3206511A CA3206511A CA3206511A1 CA 3206511 A1 CA3206511 A1 CA 3206511A1 CA 3206511 A CA3206511 A CA 3206511A CA 3206511 A CA3206511 A CA 3206511A CA 3206511 A1 CA3206511 A1 CA 3206511A1
- Authority
- CA
- Canada
- Prior art keywords
- bead
- primer
- flow cell
- sequence
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/113—Denaturating agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163143680P | 2021-01-29 | 2021-01-29 | |
| US63/143,680 | 2021-01-29 | ||
| PCT/US2022/014329 WO2022165188A1 (en) | 2021-01-29 | 2022-01-28 | Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3206511A1 true CA3206511A1 (en) | 2022-08-04 |
Family
ID=80461483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3206511A Pending CA3206511A1 (en) | 2021-01-29 | 2022-01-28 | Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240117416A1 (https=) |
| EP (1) | EP4284944A1 (https=) |
| JP (1) | JP2024505508A (https=) |
| KR (1) | KR20230154303A (https=) |
| CN (1) | CN117043352A (https=) |
| AU (1) | AU2022212112A1 (https=) |
| CA (1) | CA3206511A1 (https=) |
| WO (1) | WO2022165188A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023023638A1 (en) * | 2021-08-20 | 2023-02-23 | Singular Genomics Systems, Inc. | Chemical and thermal assisted nucleic acid amplification methods |
| EP4634400A1 (en) * | 2022-12-14 | 2025-10-22 | Illumina, Inc. | Systems and methods for capture and enrichment of clustered beads on flow cell substrates |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| CA2579150C (en) | 2004-09-17 | 2014-11-25 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| CA2648149A1 (en) | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| US9334531B2 (en) * | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
| EP3305400A3 (en) | 2011-10-28 | 2018-06-06 | Illumina, Inc. | Microarray fabrication system and method |
| EP3366348B1 (en) | 2012-01-16 | 2023-08-23 | Greatbatch Ltd. | Emi filtered co-connected hermetic feedthrough, feedthrough capacitor and leadwire assembly for an active implantable medical device |
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| DK3017065T3 (en) | 2013-07-01 | 2018-11-26 | Illumina Inc | Catalyst-free Surface functionalization and polymer grafting |
| GB201410646D0 (en) * | 2014-06-14 | 2014-07-30 | Illumina Cambridge Ltd | Methods of increasing sequencing accuracy |
| WO2016066586A1 (en) | 2014-10-31 | 2016-05-06 | Illumina Cambridge Limited | Novel polymers and dna copolymer coatings |
| SG11201911869XA (en) * | 2017-08-01 | 2020-01-30 | Illumina Inc | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
| US10981679B2 (en) * | 2017-08-04 | 2021-04-20 | Rocket Lab Usa, Inc. | Satellite deployer door release mechanism |
| IL281664B2 (en) * | 2019-01-11 | 2024-07-01 | Illumina Cambridge Ltd | Complex surface-bound transposome complexes |
-
2022
- 2022-01-28 EP EP22704678.6A patent/EP4284944A1/en active Pending
- 2022-01-28 AU AU2022212112A patent/AU2022212112A1/en active Pending
- 2022-01-28 CA CA3206511A patent/CA3206511A1/en active Pending
- 2022-01-28 US US18/274,974 patent/US20240117416A1/en active Pending
- 2022-01-28 JP JP2023545752A patent/JP2024505508A/ja active Pending
- 2022-01-28 WO PCT/US2022/014329 patent/WO2022165188A1/en not_active Ceased
- 2022-01-28 KR KR1020237027770A patent/KR20230154303A/ko active Pending
- 2022-01-28 CN CN202280020213.3A patent/CN117043352A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN117043352A (zh) | 2023-11-10 |
| KR20230154303A (ko) | 2023-11-07 |
| AU2022212112A1 (en) | 2023-08-17 |
| US20240117416A1 (en) | 2024-04-11 |
| JP2024505508A (ja) | 2024-02-06 |
| EP4284944A1 (en) | 2023-12-06 |
| WO2022165188A1 (en) | 2022-08-04 |
| AU2022212112A9 (en) | 2024-10-17 |
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