JP2024099814A5 - - Google Patents
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- JP2024099814A5 JP2024099814A5 JP2024074647A JP2024074647A JP2024099814A5 JP 2024099814 A5 JP2024099814 A5 JP 2024099814A5 JP 2024074647 A JP2024074647 A JP 2024074647A JP 2024074647 A JP2024074647 A JP 2024074647A JP 2024099814 A5 JP2024099814 A5 JP 2024099814A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- nucleotide
- free
- free process
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims description 47
- 125000003729 nucleotide group Chemical group 0.000 claims description 32
- 150000001768 cations Chemical class 0.000 claims description 26
- 150000003839 salts Chemical class 0.000 claims description 16
- 230000002255 enzymatic effect Effects 0.000 claims description 13
- 238000003786 synthesis reaction Methods 0.000 claims description 13
- 230000006820 DNA synthesis Effects 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- 229910052792 caesium Inorganic materials 0.000 claims description 9
- 239000002773 nucleotide Substances 0.000 claims description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 8
- -1 lithium nucleotide salts Chemical class 0.000 claims description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims description 7
- 229910001415 sodium ion Inorganic materials 0.000 claims description 7
- 229910052751 metal Inorganic materials 0.000 claims description 6
- 239000002184 metal Substances 0.000 claims description 6
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 claims description 5
- 239000006174 pH buffer Substances 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 4
- 239000011541 reaction mixture Substances 0.000 claims description 4
- 229910052701 rubidium Inorganic materials 0.000 claims description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- 150000003863 ammonium salts Chemical class 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 102000016559 DNA Primase Human genes 0.000 claims description 2
- 108010092681 DNA Primase Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052790 beryllium Inorganic materials 0.000 claims description 2
- ATBAMAFKBVZNFJ-UHFFFAOYSA-N beryllium atom Chemical compound [Be] ATBAMAFKBVZNFJ-UHFFFAOYSA-N 0.000 claims description 2
- NCMHKCKGHRPLCM-UHFFFAOYSA-N caesium(1+) Chemical compound [Cs+] NCMHKCKGHRPLCM-UHFFFAOYSA-N 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 150000001793 charged compounds Polymers 0.000 claims description 2
- 239000003398 denaturant Substances 0.000 claims description 2
- 229910052730 francium Inorganic materials 0.000 claims description 2
- KLMCZVJOEAUDNE-UHFFFAOYSA-N francium atom Chemical compound [Fr] KLMCZVJOEAUDNE-UHFFFAOYSA-N 0.000 claims description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- IGLNJRXAVVLDKE-UHFFFAOYSA-N rubidium atom Chemical compound [Rb] IGLNJRXAVVLDKE-UHFFFAOYSA-N 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052712 strontium Inorganic materials 0.000 claims description 2
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 239000004094 surface-active agent Substances 0.000 claims description 2
- 230000002194 synthesizing effect Effects 0.000 claims description 2
- 229910052723 transition metal Inorganic materials 0.000 claims description 2
- 150000003624 transition metals Chemical class 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052783 alkali metal Inorganic materials 0.000 claims 1
- 150000001340 alkali metals Chemical class 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1813429.6A GB201813429D0 (en) | 2018-08-17 | 2018-08-17 | Synthesis of DNA with improved yield |
| GB1813429.6 | 2018-08-17 | ||
| JP2021507969A JP7548581B2 (ja) | 2018-08-17 | 2019-08-16 | 収量が改善されたｄｎａの合成 |
| PCT/GB2019/052307 WO2020035698A1 (en) | 2018-08-17 | 2019-08-16 | Synthesis of dna with improved yield |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021507969A Division JP7548581B2 (ja) | 2018-08-17 | 2019-08-16 | 収量が改善されたｄｎａの合成 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024099814A JP2024099814A (ja) | 2024-07-25 |
| JP2024099814A5 true JP2024099814A5 (enExample) | 2025-02-03 |
Family
ID=63668172
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021507969A Active JP7548581B2 (ja) | 2018-08-17 | 2019-08-16 | 収量が改善されたｄｎａの合成 |
| JP2024074647A Pending JP2024099814A (ja) | 2018-08-17 | 2024-05-02 | 収量が改善されたｄｎａの合成 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021507969A Active JP7548581B2 (ja) | 2018-08-17 | 2019-08-16 | 収量が改善されたｄｎａの合成 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20210301312A1 (enExample) |
| EP (1) | EP3837382A1 (enExample) |
| JP (2) | JP7548581B2 (enExample) |
| KR (1) | KR20210084431A (enExample) |
| CN (1) | CN113056565A (enExample) |
| AU (1) | AU2019321778B2 (enExample) |
| BR (1) | BR112021002764A2 (enExample) |
| CA (1) | CA3109755A1 (enExample) |
| GB (1) | GB201813429D0 (enExample) |
| IL (1) | IL280817B2 (enExample) |
| MX (1) | MX2021001729A (enExample) |
| SG (1) | SG11202101548YA (enExample) |
| WO (1) | WO2020035698A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB202002068D0 (en) * | 2020-02-14 | 2020-04-01 | Touchlight Ip Ltd | DNA synthesis yield improvements |
| GB202111742D0 (en) | 2021-08-16 | 2021-09-29 | Touchlight Ip Ltd | Improved process |
| US20250027107A1 (en) | 2021-10-18 | 2025-01-23 | Flagship Pioneering Innovations Vii, Llc | Dna compositions and related methods |
| GB202319998D0 (en) | 2023-12-22 | 2024-02-07 | Touchlight Ip Ltd | duplexing method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8435774B2 (en) * | 2006-06-28 | 2013-05-07 | Qiagen Gmbh | Enhancing reactivation of thermostable reversibly inactivated enzymes |
| US8986930B2 (en) * | 2010-07-12 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Sequencing reactions with alkali metal cations for pulse width control |
| CN103649333B (zh) * | 2011-04-20 | 2017-02-15 | 梅撒生物科技公司 | 用于核酸检测和鉴定的集成装置 |
| EP2798089B1 (en) * | 2011-12-30 | 2018-05-23 | Bio-rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| US9587263B2 (en) * | 2014-03-26 | 2017-03-07 | General Electric Company | Isothermal amplification under low salt condition |
| GB201415789D0 (en) * | 2014-09-05 | 2014-10-22 | Touchlight Genetics Ltd | Synthesis of DNA |
-
2018
- 2018-08-17 GB GBGB1813429.6A patent/GB201813429D0/en not_active Ceased
-
2019
- 2019-08-16 SG SG11202101548YA patent/SG11202101548YA/en unknown
- 2019-08-16 IL IL280817A patent/IL280817B2/en unknown
- 2019-08-16 KR KR1020217007998A patent/KR20210084431A/ko active Pending
- 2019-08-16 CA CA3109755A patent/CA3109755A1/en active Pending
- 2019-08-16 JP JP2021507969A patent/JP7548581B2/ja active Active
- 2019-08-16 CN CN201980067811.4A patent/CN113056565A/zh active Pending
- 2019-08-16 WO PCT/GB2019/052307 patent/WO2020035698A1/en not_active Ceased
- 2019-08-16 US US17/268,721 patent/US20210301312A1/en not_active Abandoned
- 2019-08-16 EP EP19755959.4A patent/EP3837382A1/en active Pending
- 2019-08-16 AU AU2019321778A patent/AU2019321778B2/en active Active
- 2019-08-16 MX MX2021001729A patent/MX2021001729A/es unknown
- 2019-08-16 BR BR112021002764-5A patent/BR112021002764A2/pt unknown
-
2024
- 2024-05-02 JP JP2024074647A patent/JP2024099814A/ja active Pending
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