JP2023550693A - 光退色情報を使用する単一分子検出システム - Google Patents

光退色情報を使用する単一分子検出システム Download PDF

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Publication number
JP2023550693A
JP2023550693A JP2023525609A JP2023525609A JP2023550693A JP 2023550693 A JP2023550693 A JP 2023550693A JP 2023525609 A JP2023525609 A JP 2023525609A JP 2023525609 A JP2023525609 A JP 2023525609A JP 2023550693 A JP2023550693 A JP 2023550693A
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Prior art keywords
reference dye
sample
biomolecules
integrated device
individual
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JP2023525609A
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Japanese (ja)
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JP2023550693A5 (enExample
Inventor
プレストン、カイル
リード、ブライアン
シュミッド、ジェラード
シー、シンホア
ホワン、ハイドン
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Quantum Si Inc
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Quantum Si Inc
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Publication of JP2023550693A5 publication Critical patent/JP2023550693A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2023525609A 2020-10-27 2021-10-26 光退色情報を使用する単一分子検出システム Withdrawn JP2023550693A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063106325P 2020-10-27 2020-10-27
US63/106,325 2020-10-27
US202163165798P 2021-03-25 2021-03-25
US63/165,798 2021-03-25
US202163252906P 2021-10-06 2021-10-06
US63/252,906 2021-10-06
PCT/US2021/056707 WO2022093861A1 (en) 2020-10-27 2021-10-26 Single molecule detection system using photobleaching information

Publications (2)

Publication Number Publication Date
JP2023550693A true JP2023550693A (ja) 2023-12-05
JP2023550693A5 JP2023550693A5 (enExample) 2024-11-05

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JP2023525609A Withdrawn JP2023550693A (ja) 2020-10-27 2021-10-26 光退色情報を使用する単一分子検出システム

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US (2) US20220128471A1 (enExample)
EP (1) EP4221894A4 (enExample)
JP (1) JP2023550693A (enExample)
KR (1) KR20230097103A (enExample)
AU (1) AU2021372457A1 (enExample)
CA (1) CA3199746A1 (enExample)
WO (1) WO2022093861A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025126814A1 (ja) * 2023-12-12 2025-06-19 東ソー株式会社 測定領域を特定する方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112020000799A2 (pt) * 2017-07-24 2020-07-14 Quantum-Si Incorporated estruturas fotônicas de rejeição óptica

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008514955A (ja) * 2004-09-28 2008-05-08 シンギュレックス・インコーポレイテッド サンプル分析システムおよび方法
WO2008123180A1 (ja) * 2007-03-23 2008-10-16 National University Corporation Tokyo Medical And Dental University 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット
US20110281740A1 (en) * 2008-06-30 2011-11-17 Joseph Beechem Methods for Real Time Single Molecule Sequencing
CA2839702C (en) * 2011-06-23 2021-04-20 Board Of Regents, The University Of Texas System Identifying peptides at the single molecule level
WO2015074001A1 (en) * 2013-11-17 2015-05-21 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US10174363B2 (en) * 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN113528623A (zh) * 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
US11016084B2 (en) * 2015-10-05 2021-05-25 The University Of North Carolina At Chapel Hill Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports
CA3105715A1 (en) * 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
WO2020016887A1 (en) * 2018-07-16 2020-01-23 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Optical devices and methods for measuring efficiency of the fӧrster resonance energy transfer

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025126814A1 (ja) * 2023-12-12 2025-06-19 東ソー株式会社 測定領域を特定する方法

Also Published As

Publication number Publication date
US20220128469A1 (en) 2022-04-28
WO2022093861A1 (en) 2022-05-05
KR20230097103A (ko) 2023-06-30
EP4221894A4 (en) 2024-10-23
EP4221894A1 (en) 2023-08-09
CA3199746A1 (en) 2022-05-05
AU2021372457A1 (en) 2023-06-15
US20220128471A1 (en) 2022-04-28

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