JP2023540782A - 遺伝子ライブラリーの高効率構築および遺伝子解析のためのアダプターおよび方法 - Google Patents

遺伝子ライブラリーの高効率構築および遺伝子解析のためのアダプターおよび方法 Download PDF

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JP2023540782A
JP2023540782A JP2023515279A JP2023515279A JP2023540782A JP 2023540782 A JP2023540782 A JP 2023540782A JP 2023515279 A JP2023515279 A JP 2023515279A JP 2023515279 A JP2023515279 A JP 2023515279A JP 2023540782 A JP2023540782 A JP 2023540782A
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dna
adapter
multifunctional
region
nucleotides
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JP2023540782A5 (enExample
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ジアンナン グオ，
ジェニファー ヘルナンデス，
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レゾリューション バイオサイエンス， インコーポレイテッド
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Publication of JP2023540782A publication Critical patent/JP2023540782A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023515279A 2020-09-08 2021-09-08 遺伝子ライブラリーの高効率構築および遺伝子解析のためのアダプターおよび方法 Pending JP2023540782A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063075543P 2020-09-08 2020-09-08
US63/075,543 2020-09-08
PCT/US2021/049448 WO2022055984A1 (en) 2020-09-08 2021-09-08 Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis

Publications (2)

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JP2023540782A true JP2023540782A (ja) 2023-09-26
JP2023540782A5 JP2023540782A5 (enExample) 2024-09-04

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JP2023515279A Pending JP2023540782A (ja) 2020-09-08 2021-09-08 遺伝子ライブラリーの高効率構築および遺伝子解析のためのアダプターおよび方法

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US (1) US20220073906A1 (enExample)
EP (1) EP4211246B1 (enExample)
JP (1) JP2023540782A (enExample)
KR (1) KR20230065295A (enExample)
CN (1) CN116490613A (enExample)
AU (1) AU2021340632A1 (enExample)
CA (1) CA3189103A1 (enExample)
DK (1) DK4211246T3 (enExample)
ES (1) ES3045482T3 (enExample)
HU (1) HUE072019T2 (enExample)
PL (1) PL4211246T3 (enExample)
PT (1) PT4211246T (enExample)
WO (1) WO2022055984A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105531375B (zh) 2012-12-10 2020-03-03 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
KR102505122B1 (ko) 2016-08-25 2023-03-08 레졸루션 바이오사이언스, 인크. Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법
CN115029413A (zh) * 2022-05-17 2022-09-09 翌圣生物科技(上海)股份有限公司 一种dna和/或rna快速建库的方法和试剂盒
WO2024049276A1 (ko) * 2022-09-01 2024-03-07 주식회사 키오믹스 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017525371A (ja) * 2014-08-22 2017-09-07 レゾリューション バイオサイエンス， インコーポレイテッド 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法
JP2019504618A (ja) * 2015-11-11 2019-02-21 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
WO2019140201A1 (en) * 2018-01-12 2019-07-18 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
JP2020516281A (ja) * 2017-04-14 2020-06-11 ガーダント ヘルス， インコーポレイテッド 試料核酸にアダプターを付着する方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214557A1 (en) * 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
GB201615486D0 (en) * 2016-09-13 2016-10-26 Inivata Ltd Methods for labelling nucleic acids
JP7048609B2 (ja) * 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
JP6982087B2 (ja) * 2017-01-27 2021-12-17 インテグレイテッド ディエヌエイ テクノロジイズ, インコーポレイテッドIntegrated Dna Technologies, Incorporated 競合的鎖置換を利用する次世代シーケンシング（ｎｇｓ）ライブラリーの構築
AU2019384154B2 (en) * 2018-11-21 2025-11-20 Agilent Technologies, Inc. Methods for targeted nucleic acid library formation
US11555185B2 (en) 2018-12-19 2023-01-17 New England Biolabs, Inc. Target enrichment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017525371A (ja) * 2014-08-22 2017-09-07 レゾリューション バイオサイエンス， インコーポレイテッド 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法
JP2019504618A (ja) * 2015-11-11 2019-02-21 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
JP2020516281A (ja) * 2017-04-14 2020-06-11 ガーダント ヘルス， インコーポレイテッド 試料核酸にアダプターを付着する方法
WO2019140201A1 (en) * 2018-01-12 2019-07-18 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid

Also Published As

Publication number Publication date
HUE072019T2 (hu) 2025-10-28
CN116490613A (zh) 2023-07-25
CA3189103A1 (en) 2022-03-17
PL4211246T3 (pl) 2025-10-20
DK4211246T3 (da) 2025-08-18
EP4211246B1 (en) 2025-07-23
EP4211246A1 (en) 2023-07-19
AU2021340632A1 (en) 2023-03-16
PT4211246T (pt) 2025-07-30
US20220073906A1 (en) 2022-03-10
WO2022055984A1 (en) 2022-03-17
EP4211246A4 (en) 2024-07-24
KR20230065295A (ko) 2023-05-11
ES3045482T3 (en) 2025-11-28

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