JP2023537397A - 標的化された核酸の切断方法 - Google Patents

標的化された核酸の切断方法 Download PDF

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JP2023537397A
JP2023537397A JP2023509684A JP2023509684A JP2023537397A JP 2023537397 A JP2023537397 A JP 2023537397A JP 2023509684 A JP2023509684 A JP 2023509684A JP 2023509684 A JP2023509684 A JP 2023509684A JP 2023537397 A JP2023537397 A JP 2023537397A
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nucleic acid
moiety
group
rna
cell
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JP2023537397A5 (https=
Inventor
ゴンカロ ベルナルデス
シギタス ミクティス
コンスタンティノス ティゼレピス
マドカ ハゼミ
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Cambridge Enterprise Ltd
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Cambridge Enterprise Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
JP2023509684A 2020-08-12 2021-08-12 標的化された核酸の切断方法 Pending JP2023537397A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20386040 2020-08-12
EP20386040.8 2020-08-12
PCT/EP2021/072517 WO2022034177A1 (en) 2020-08-12 2021-08-12 Method for targeted nucleic acid cleavage

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JP2023537397A true JP2023537397A (ja) 2023-08-31
JP2023537397A5 JP2023537397A5 (https=) 2024-08-14

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US (1) US20230272460A1 (https=)
EP (1) EP4196610A1 (https=)
JP (1) JP2023537397A (https=)
WO (1) WO2022034177A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202115540D0 (en) * 2021-10-28 2021-12-15 Cambridge Entpr Ltd Methods for nucleic acid cleavage
CN114874639B (zh) * 2022-05-12 2023-07-14 大连理工大学 一类新型半花菁近红外荧光染料，其合成方法及应用
CN115677548B (zh) * 2022-11-16 2024-04-09 湖南农业大学 一种硒代蛋氨酸的高效合成方法
GB202300884D0 (en) * 2023-01-20 2023-03-08 Cambridge Entpr Ltd Compounds for nucleic acid cleavage
GB202300886D0 (en) * 2023-01-20 2023-03-08 Cambridge Entpr Ltd Compounds for nucleic acid cleavage

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110462A (en) * 1999-03-03 2000-08-29 The Scripps Research Institute Enzymatic DNA molecules that contain modified nucleotides
WO2020127959A1 (en) * 2018-12-21 2020-06-25 Curevac Ag Methods for rna analysis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005286722B2 (en) * 2004-09-21 2010-12-02 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
EP3464594A1 (en) * 2016-06-01 2019-04-10 Kws Saat Se Hybrid nucleic acid sequences for genome engineering
SG11201901841TA (en) * 2016-09-02 2019-03-28 Arrowhead Pharmaceuticals Inc Targeting ligands
US20190202856A1 (en) * 2017-12-29 2019-07-04 Sigma-Aldrich Co. Llc Engineered crispr proteins for covalent tagging nucleic acids
WO2021155297A1 (en) * 2020-01-29 2021-08-05 President And Fellows Of Harvard College Methods for labeling and targeting cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110462A (en) * 1999-03-03 2000-08-29 The Scripps Research Institute Enzymatic DNA molecules that contain modified nucleotides
WO2020127959A1 (en) * 2018-12-21 2020-06-25 Curevac Ag Methods for rna analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KAORU USIZIMA; ET AL: "SITE-SPECIFIC CLEAVAGE OF TRNA BY IMIDAZOLE AND/OR PRIMARY AMINE GROUPS BOUND AT 以下備考", BIOCHIMICA ET BIOPHYSICA ACTA, vol. VOL:1379,NR:2, JPN5024002663, 2 February 1998 (1998-02-02), NL, pages 217 - 223, ISSN: 0005661490 *

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EP4196610A1 (en) 2023-06-21
US20230272460A1 (en) 2023-08-31

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