JP2023521086A - 凍結乾燥間葉系幹細胞由来セクレトーム及びその使用 - Google Patents

凍結乾燥間葉系幹細胞由来セクレトーム及びその使用 Download PDF

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JP2023521086A
JP2023521086A JP2022561111A JP2022561111A JP2023521086A JP 2023521086 A JP2023521086 A JP 2023521086A JP 2022561111 A JP2022561111 A JP 2022561111A JP 2022561111 A JP2022561111 A JP 2022561111A JP 2023521086 A JP2023521086 A JP 2023521086A
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lyophilized
msc
secretome
composition
msc secretome
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JP2023521086A5 (https=
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アルフォード，スペンサー
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Combangio Inc
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Combangio Inc
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JP2022561111A 2020-04-07 2021-04-06 凍結乾燥間葉系幹細胞由来セクレトーム及びその使用 Pending JP2023521086A (ja)

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US202063006680P 2020-04-07 2020-04-07
US63/006,680 2020-04-07
PCT/US2021/026059 WO2021207282A2 (en) 2020-04-07 2021-04-06 Lyophilized mesenchymal stem cell derived secretome and uses thereof

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JP2023521086A true JP2023521086A (ja) 2023-05-23
JP2023521086A5 JP2023521086A5 (https=) 2024-04-05

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US (2) US12186430B2 (https=)
EP (1) EP4132546A4 (https=)
JP (1) JP2023521086A (https=)
KR (1) KR20230035517A (https=)
CN (1) CN115968400A (https=)
AU (1) AU2021251785A1 (https=)
WO (1) WO2021207282A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3801028A4 (en) 2018-05-30 2022-04-13 Direct Biologics LLC A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use
US12569517B2 (en) 2019-02-07 2026-03-10 Direct Biologics, Llc Method for treating osteoarthritis with mesenchymal stem cell exosomes
US12213995B2 (en) 2019-07-18 2025-02-04 Direct Biologics, Llc Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use
WO2021207282A2 (en) 2020-04-07 2021-10-14 Combangio, Inc. Lyophilized mesenchymal stem cell derived secretome and uses thereof
KR20230004709A (ko) 2020-04-22 2023-01-06 다이렉트 바이오로직스 엘엘씨 감염성 질환과 연관된 염증성 병태를 치료하기 위한 방법 및 조성물
US20240000891A1 (en) * 2020-12-08 2024-01-04 The Board Of Trustees Of The Leland Stanford Junior University Growth and differentiation factor 15 for treatment of proliferative vitreoretinopathy therapy
CN114990059B (zh) * 2022-06-30 2024-04-26 阿谷巴(杭州)生物科技发展有限公司 一种脐带间充质干细胞冻干粉的制备方法
KR20250044282A (ko) * 2022-07-08 2025-03-31 컴반지오, 인크. 분비체 활성 분석을 위한 방법 및 검정
US12502407B2 (en) 2024-04-25 2025-12-23 Direct Biologics, Llc Treatment of fistula with bone marrow mesenchymal stem cell derived extracellular vesicles

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018078524A1 (en) * 2016-10-28 2018-05-03 Pharmaexceed S.R.L. Process for isolating and lyophilizing extracellular vesicles

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU766826B2 (en) * 1998-07-30 2003-10-23 Government of The United States of America, as represented by The Secretary Department of Health & Human Services, The National Institutes of Health, The Thymosin beta4 promotes wound repair
US6372494B1 (en) 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
KR20090115984A (ko) * 2001-11-09 2009-11-10 아르테셀 사이언스, 인크. 지방 조직에서 유래된 기질세포의 내분비 췌장 분화 및 이의 용도
JP2007509989A (ja) * 2003-10-31 2007-04-19 ポイント バイオメディカル コーポレイション 超音波造影剤として有用な再構成可能なミクロスフィア組成物
US20060165667A1 (en) 2004-12-03 2006-07-27 Case Western Reserve University Novel methods, compositions and devices for inducing neovascularization
US20060222634A1 (en) 2005-03-31 2006-10-05 Clarke Diana L Amnion-derived cell compositions, methods of making and uses thereof
WO2008020815A1 (en) 2006-08-15 2008-02-21 Agency For Science, Technology And Research Mesenchymal stem cell conditioned medium
US8871198B2 (en) 2006-03-29 2014-10-28 Stemnion, Inc. Methods related to wound healing
JP2009526858A (ja) 2006-02-15 2009-07-23 ティカ レーケメデル アーベー コルチコステロイド溶液を製造する方法
WO2008024996A2 (en) 2006-08-24 2008-02-28 Johnstone Brian H Production of neural protective and regenerative factors from stem cells and treatment of nervous system conditions therewith
US20090054339A1 (en) 2007-08-22 2009-02-26 Marshall Vivienne S Novel cellular factor-containing solution compositions
WO2009045359A1 (en) 2007-10-01 2009-04-09 Stemnion, Inc. Skin replacement compositions and methods
EP2254586B1 (en) 2008-02-22 2015-04-08 Agency For Science, Technology And Research (A*star) Mesenchymal stem cell particles
US20110256110A1 (en) * 2008-10-24 2011-10-20 Children's Hospital Los Angeles Amniotic fluid cells and uses thereof
US20110158958A1 (en) 2009-12-07 2011-06-30 Sing George L Methods for treating ophthalmic disorders, diseases and injuries
WO2010110768A1 (en) 2009-03-23 2010-09-30 Regenetech, Inc. Composition and method for producing composition for constructing tissue, and tissue construct
DK2451964T3 (en) 2009-07-10 2018-04-30 Histogen Inc Conditioned medium and extracellular matrix compositions from cells grown under hypoxic conditions
US20110206647A1 (en) 2010-02-25 2011-08-25 Abt Holding Company Modulation of Angiogenesis
WO2011127090A1 (en) 2010-04-05 2011-10-13 Medstar Health Research Institute, Inc. Conditioned medium obtained form stem cells and its use in therapy
FR2974201B1 (fr) 2011-04-18 2013-04-12 Inside Secure Circuit de multiplication de montgomery
US20120276215A1 (en) 2011-04-26 2012-11-01 Riordan Neil H Therapeutic Conditioned Media
EP2723364A1 (en) 2011-06-24 2014-04-30 National University of Ireland Galway Cell secreted proteins for the treatment of myocardial infarction
ES2661587T3 (es) * 2011-08-10 2018-04-02 Depuy Synthes Products Llc Tratamiento de enfermedad vascular periférica usando células derivadas de tejido del cordón umbilical
ITTO20111183A1 (it) 2011-12-21 2013-06-22 Univ Degli Studi Torino Mezzo condizionato ottenuto da cellule staminali mesenchimali placentari e suo uso nel trattamento terapeutico della preeclampsia
HK1202581A1 (en) 2012-02-13 2015-10-02 Gamida-Cell Ltd. Mesenchymal stem cells conditioned medium and methods of generating and using the same
ITTO20120859A1 (it) 2012-10-02 2014-04-03 Univ Degli Studi Torino Nuova applicazione terapeutica di un mezzo condizionato da cellule staminali mesenchimali placentari
EP2943069B1 (en) 2013-01-09 2018-08-15 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
US20140242043A1 (en) 2013-02-23 2014-08-28 Stemnion, Inc. Methods for preventing or treating optic neuritis
US20140255355A1 (en) 2013-03-07 2014-09-11 Stemnion, Inc. Methods for treating blepharitis
CA2924562A1 (en) 2013-10-17 2015-04-23 Sentiss Pharma Private Limited Preservative-free ophthalmic pharmaceutical formulation
CL2013003066A1 (es) 2013-10-22 2014-07-25 Univ Chile Composicion para tratamiento de heridas porque comprende matriz soporte y células troncalesmesenquiámicas de gelatina de wharton; metodo para tratar heridas que comprende aplicar dicha composicion
US9636364B2 (en) 2013-12-04 2017-05-02 Stemnion, Inc. Methods for treating ocular contusion and blunt injury and traumatic injury to the optic nerve
US9464272B2 (en) 2013-12-12 2016-10-11 Stemnion, Inc. Cell-derived composition
US20150335712A1 (en) 2014-05-21 2015-11-26 Stemnion, Inc. Treatment of Diseases and Conditions Caused by Increased Vascular Permeability
WO2016082882A1 (en) 2014-11-27 2016-06-02 Med Cell Europe Ag Secretomes and method for producing secretomes
EP3224348B1 (en) 2014-11-27 2021-07-14 Med Cell Bahamas Ltd. Secretomes and method for producing secretomes
US20160158293A1 (en) * 2014-12-05 2016-06-09 Janssen Biotech, Inc. Treatment of Ocular Conditions Using Progenitor Cells
US20170080033A1 (en) 2014-12-16 2017-03-23 Janssen Biotech, Inc. Treatment of retinal degeneration using progenitor cells
US20180327713A1 (en) 2014-12-16 2018-11-15 Janssen Biotech, Inc. Treatment of retinal degeneration using progenitor cells
SG11201704668PA (en) 2014-12-16 2017-07-28 Janssen Biotech Inc Treatment of retinal degeneration using progenitor cells
KR101566450B1 (ko) 2015-04-03 2015-11-05 (유)스템메디케어 중간엽 줄기세포에서 단백질의 대량 생산 방법
TWI741980B (zh) 2015-04-07 2021-10-11 大陸商四川藍光英諾生物科技股份有限公司 一種生物磚及其用途
US20160361253A1 (en) 2015-06-13 2016-12-15 Noveome Biotherapeutics, Inc. Novel Methods for Delivering Therapeutic Agents to the Eye via Nasal Passages
EP3317397B1 (en) 2015-07-02 2019-09-25 Med Cell Bahamas Ltd. Secretomes and method for producing secretomes
EP3328413B1 (en) 2015-07-29 2024-09-04 Indiana University Research and Technology Corporation Materials and methods for treating and evaluating ischemic and/or reperfusion-injured tissue and/or tissue susceptible to same
US20190046576A1 (en) 2016-02-12 2019-02-14 Cell Care Therapeutics Adipose tissue derived mesenchymal stromal cell conditioned media and methods of making and using the same
KR20170111057A (ko) 2016-03-25 2017-10-12 (주)안트로젠 면역 질환 또는 염증성 질환의 예방 또는 치료용 중간엽줄기세포 배양액 및 이의 제조방법
RU2708329C2 (ru) 2016-05-31 2019-12-05 Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" Материал стволовых клеток, композиции и способы применения
WO2017217967A1 (en) 2016-06-13 2017-12-21 Noveome Biotherapeutics, Inc. Novel methods for delivering therapeutic agents to the eye via nasal passages
US20180028570A1 (en) 2016-08-01 2018-02-01 Ucl Business Plc Angiogenic Factors
KR20180024223A (ko) 2016-08-29 2018-03-08 사회복지법인 삼성생명공익재단 중간엽 줄기세포 분비 단백질을 포함하는 말초신경 세포 증식 촉진용 조성물
US20190231694A1 (en) 2016-10-12 2019-08-01 Agency For Science, Technology And Research Method for lyophilising an exosome
WO2018102174A1 (en) 2016-12-01 2018-06-07 Janssen Biotech, Inc. Treatment of retinal degeneration using progenitor cells
JP2020505345A (ja) 2017-01-16 2020-02-20 セルズ フォー セルズ エセ．アー． 乏血小板血漿（ｐｐｐ）に封入された実質的に純粋な多能性間質細胞集団を含む組成物
US20180271914A1 (en) 2017-03-21 2018-09-27 Noveome Biotherapeutics, Inc. Methods for Reducing the Extent of Light-induced Tissue Inflammation and Injury
EP3624731A4 (en) 2017-05-18 2021-02-24 The Board of Trustees of the Leland Stanford Junior University TARGETED IN-SITU THERAPEUTIC ADMINISTRATION OF SECRETED FACTORS FROM STEM CELLS FOR THE TREATMENT OF DAMAGED TISSUE
EP3655525A4 (en) 2017-07-16 2021-04-21 Ramot at Tel-Aviv University Ltd. STEM CELL SECRETOME FROM HUMAN MUCCOUS
US10364270B2 (en) 2017-10-02 2019-07-30 Pro Sunfun Biotech Research And Developmment Co., Ltd. Eye-care peptide, its composition and method of using the same
CN108517002A (zh) 2018-04-20 2018-09-11 命之本源医疗科技（北京）有限公司 一种干细胞培养上清分泌蛋白的稳定剂
EP3801028A4 (en) 2018-05-30 2022-04-13 Direct Biologics LLC A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use
TWI834808B (zh) 2019-02-19 2024-03-11 西班牙商薩爾瓦特實驗室有限公司 以單劑包裝的克氯黴唑液體組成物
US10881693B2 (en) 2019-04-09 2021-01-05 Combangio, Inc. Processes for making and using a mesenchymal stem cell derived secretome
US12213995B2 (en) 2019-07-18 2025-02-04 Direct Biologics, Llc Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use
WO2021207282A2 (en) 2020-04-07 2021-10-14 Combangio, Inc. Lyophilized mesenchymal stem cell derived secretome and uses thereof
KR20250044282A (ko) 2022-07-08 2025-03-31 컴반지오, 인크. 분비체 활성 분석을 위한 방법 및 검정

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018078524A1 (en) * 2016-10-28 2018-05-03 Pharmaexceed S.R.L. Process for isolating and lyophilizing extracellular vesicles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
STEM CELLS TRANSL. MED., vol. 8, no. 5, JPN6025007034, 2019, pages 478 - 489, ISSN: 0005743237 *

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