JP2023512985A - 改善された検出アッセイ - Google Patents

改善された検出アッセイ Download PDF

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JP2023512985A
JP2023512985A JP2022545378A JP2022545378A JP2023512985A JP 2023512985 A JP2023512985 A JP 2023512985A JP 2022545378 A JP2022545378 A JP 2022545378A JP 2022545378 A JP2022545378 A JP 2022545378A JP 2023512985 A JP2023512985 A JP 2023512985A
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activity
thermostable
cas protein
cas
collateral
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JP2023512985A5 (https=
Inventor
ウィリアム ジェレミー ブレイク，
シャン リー，
メアリー キャサリン ウィルソン，
クリスティン マリー コティッキア，
プラディープ ラメシュ，
ブレンダン ジョン マニング，
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シャーロック バイオサイエンシーズ， インコーポレイテッド
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Publication of JP2023512985A publication Critical patent/JP2023512985A/ja
Publication of JP2023512985A5 publication Critical patent/JP2023512985A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
JP2022545378A 2020-01-27 2021-01-27 改善された検出アッセイ Pending JP2023512985A (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US202062966527P 2020-01-27 2020-01-27
US62/966,527 2020-01-27
US202062967536P 2020-01-29 2020-01-29
US62/967,536 2020-01-29
US202062970159P 2020-02-04 2020-02-04
US62/970,159 2020-02-04
US202063038710P 2020-06-12 2020-06-12
US63/038,710 2020-06-12
US202163139267P 2021-01-19 2021-01-19
US63/139,267 2021-01-19
PCT/US2021/015306 WO2021154866A1 (en) 2020-01-27 2021-01-27 Improved detection assays

Publications (2)

Publication Number Publication Date
JP2023512985A true JP2023512985A (ja) 2023-03-30
JP2023512985A5 JP2023512985A5 (https=) 2024-01-31

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ID=74858746

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JP2022545378A Pending JP2023512985A (ja) 2020-01-27 2021-01-27 改善された検出アッセイ

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US (3) US20230183783A1 (https=)
EP (1) EP4097250A1 (https=)
JP (1) JP2023512985A (https=)
KR (1) KR20220131939A (https=)
AU (1) AU2021212731A1 (https=)
BR (1) BR112022014777A2 (https=)
CA (1) CA3168830A1 (https=)
IL (1) IL295011A (https=)
MX (1) MX2022009212A (https=)
TW (1) TW202142698A (https=)
WO (1) WO2021154866A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US12534743B2 (en) 2018-08-31 2026-01-27 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US12545910B2 (en) 2018-08-31 2026-02-10 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US20230235414A1 (en) * 2020-05-27 2023-07-27 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
EP4274890A4 (en) * 2021-01-07 2024-11-27 Inscripta, Inc. MAD-NUCLEASES
US20250277199A1 (en) 2021-03-02 2025-09-04 BRAIN Biotech AG Novel crispr-cas nucleases from metagenomes
TW202321454A (zh) * 2021-07-26 2023-06-01 美商夏洛克生物科學公司 經改良之ｃｒｉｓｐｒ－ｃａｓ技術
EP4423263A4 (en) * 2021-10-29 2026-03-04 Mammoth Biosciences Inc EFFECTOR PROTEINS, COMPOSITIONS, SYSTEMS, DEVICES, KITS AND METHODS FOR THERECOMMENDATION
WO2024005863A1 (en) * 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) * 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US20260043073A1 (en) 2022-07-01 2026-02-12 Sherlock Biosciences, Inc. Ambient temperature nucleic acid amplification and detection
WO2024218780A1 (en) * 2023-04-20 2024-10-24 Limay Bio Sa Mehtods and systems for accelerated crispr-cas reaction-based detection of nucleic acids

Citations (1)

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WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems

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WO2016100955A2 (en) * 2014-12-20 2016-06-23 Identifygenomics, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
CN110506128A (zh) 2016-12-09 2019-11-26 博德研究所 基于crispr效应系统的诊断
CN112501254B (zh) * 2017-07-14 2024-07-19 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US10253365B1 (en) 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
CA3086550A1 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
WO2019178428A1 (en) * 2018-03-14 2019-09-19 Arbor Biotechnologies, Inc. Novel crispr dna and rna targeting enzymes and systems
EP3931313A2 (en) * 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
CN115175996A (zh) * 2019-09-20 2022-10-11 博德研究所 新颖vi型crispr酶和系统
US11453907B2 (en) * 2020-03-23 2022-09-27 The Broad Institute, Inc. Crispr effector system based coronavirus diagnostics

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Accession: T0D7A2", CRISPR-ASSOCIATED ENDONUCLEASE CAS12B, [ONLINE], 掲載日: 2019.12.11, 検索日: 2025.3.14, DATABASE UNI, JPN6025011640, ISSN: 0005741228 *
ACS SYNTH. BIOL., vol. 8, JPN6025011642, 2019, pages 2228 - 2237, ISSN: 0005741229 *
NAT. PROTOC., vol. 14, JPN7025001202, 2019, pages 2986 - 3012, ISSN: 0005741230 *

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Publication number Publication date
KR20220131939A (ko) 2022-09-29
AU2021212731A1 (en) 2022-08-25
TW202142698A (zh) 2021-11-16
MX2022009212A (es) 2022-11-09
BR112022014777A2 (pt) 2022-09-20
IL295011A (en) 2022-09-01
US20230272458A1 (en) 2023-08-31
US20250327116A1 (en) 2025-10-23
CA3168830A1 (en) 2021-08-05
EP4097250A1 (en) 2022-12-07
US20230183783A1 (en) 2023-06-15
WO2021154866A1 (en) 2021-08-05

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