AU2021212731A1 - Improved detection assays - Google Patents

Improved detection assays Download PDF

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Publication number
AU2021212731A1
AU2021212731A1 AU2021212731A AU2021212731A AU2021212731A1 AU 2021212731 A1 AU2021212731 A1 AU 2021212731A1 AU 2021212731 A AU2021212731 A AU 2021212731A AU 2021212731 A AU2021212731 A AU 2021212731A AU 2021212731 A1 AU2021212731 A1 AU 2021212731A1
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AU
Australia
Prior art keywords
activity
thermostable
cas
cas protein
collateral
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Pending
Application number
AU2021212731A
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English (en)
Inventor
William Jeremy Blake
Christine Marie COTICCHIA
Xiang Li
Brendan John Manning
Pradeep Ramesh
Mary Katherine WILSON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sherlock Biosciences Inc
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Sherlock Biosciences Inc
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Publication date
Application filed by Sherlock Biosciences Inc filed Critical Sherlock Biosciences Inc
Publication of AU2021212731A1 publication Critical patent/AU2021212731A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
AU2021212731A 2020-01-27 2021-01-27 Improved detection assays Pending AU2021212731A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US202062966527P 2020-01-27 2020-01-27
US62/966,527 2020-01-27
US202062967536P 2020-01-29 2020-01-29
US62/967,536 2020-01-29
US202062970159P 2020-02-04 2020-02-04
US62/970,159 2020-02-04
US202063038710P 2020-06-12 2020-06-12
US63/038,710 2020-06-12
US202163139267P 2021-01-19 2021-01-19
US63/139,267 2021-01-19
PCT/US2021/015306 WO2021154866A1 (en) 2020-01-27 2021-01-27 Improved detection assays

Publications (1)

Publication Number Publication Date
AU2021212731A1 true AU2021212731A1 (en) 2022-08-25

Family

ID=74858746

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021212731A Pending AU2021212731A1 (en) 2020-01-27 2021-01-27 Improved detection assays

Country Status (11)

Country Link
US (3) US20230183783A1 (https=)
EP (1) EP4097250A1 (https=)
JP (1) JP2023512985A (https=)
KR (1) KR20220131939A (https=)
AU (1) AU2021212731A1 (https=)
BR (1) BR112022014777A2 (https=)
CA (1) CA3168830A1 (https=)
IL (1) IL295011A (https=)
MX (1) MX2022009212A (https=)
TW (1) TW202142698A (https=)
WO (1) WO2021154866A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US12534743B2 (en) 2018-08-31 2026-01-27 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US12545910B2 (en) 2018-08-31 2026-02-10 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US20230235414A1 (en) * 2020-05-27 2023-07-27 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
EP4274890A4 (en) * 2021-01-07 2024-11-27 Inscripta, Inc. MAD-NUCLEASES
US20250277199A1 (en) 2021-03-02 2025-09-04 BRAIN Biotech AG Novel crispr-cas nucleases from metagenomes
TW202321454A (zh) * 2021-07-26 2023-06-01 美商夏洛克生物科學公司 經改良之ｃｒｉｓｐｒ－ｃａｓ技術
EP4423263A4 (en) * 2021-10-29 2026-03-04 Mammoth Biosciences Inc EFFECTOR PROTEINS, COMPOSITIONS, SYSTEMS, DEVICES, KITS AND METHODS FOR THERECOMMENDATION
WO2024005863A1 (en) * 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) * 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US20260043073A1 (en) 2022-07-01 2026-02-12 Sherlock Biosciences, Inc. Ambient temperature nucleic acid amplification and detection
WO2024218780A1 (en) * 2023-04-20 2024-10-24 Limay Bio Sa Mehtods and systems for accelerated crispr-cas reaction-based detection of nucleic acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016100955A2 (en) * 2014-12-20 2016-06-23 Identifygenomics, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
CN110506128A (zh) 2016-12-09 2019-11-26 博德研究所 基于crispr效应系统的诊断
CN112501254B (zh) * 2017-07-14 2024-07-19 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US10253365B1 (en) 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
CA3086550A1 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
WO2019178428A1 (en) * 2018-03-14 2019-09-19 Arbor Biotechnologies, Inc. Novel crispr dna and rna targeting enzymes and systems
EP3931313A2 (en) * 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
CN115175996A (zh) * 2019-09-20 2022-10-11 博德研究所 新颖vi型crispr酶和系统
US11453907B2 (en) * 2020-03-23 2022-09-27 The Broad Institute, Inc. Crispr effector system based coronavirus diagnostics

Also Published As

Publication number Publication date
KR20220131939A (ko) 2022-09-29
TW202142698A (zh) 2021-11-16
MX2022009212A (es) 2022-11-09
BR112022014777A2 (pt) 2022-09-20
JP2023512985A (ja) 2023-03-30
IL295011A (en) 2022-09-01
US20230272458A1 (en) 2023-08-31
US20250327116A1 (en) 2025-10-23
CA3168830A1 (en) 2021-08-05
EP4097250A1 (en) 2022-12-07
US20230183783A1 (en) 2023-06-15
WO2021154866A1 (en) 2021-08-05

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