CA3168830A1 - Improved detection assays - Google Patents
Improved detection assaysInfo
- Publication number
- CA3168830A1 CA3168830A1 CA3168830A CA3168830A CA3168830A1 CA 3168830 A1 CA3168830 A1 CA 3168830A1 CA 3168830 A CA3168830 A CA 3168830A CA 3168830 A CA3168830 A CA 3168830A CA 3168830 A1 CA3168830 A1 CA 3168830A1
- Authority
- CA
- Canada
- Prior art keywords
- cas12
- thermostable
- cas protein
- cas
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062966527P | 2020-01-27 | 2020-01-27 | |
| US62/966,527 | 2020-01-27 | ||
| US202062967536P | 2020-01-29 | 2020-01-29 | |
| US62/967,536 | 2020-01-29 | ||
| US202062970159P | 2020-02-04 | 2020-02-04 | |
| US62/970,159 | 2020-02-04 | ||
| US202063038710P | 2020-06-12 | 2020-06-12 | |
| US63/038,710 | 2020-06-12 | ||
| US202163139267P | 2021-01-19 | 2021-01-19 | |
| US63/139,267 | 2021-01-19 | ||
| PCT/US2021/015306 WO2021154866A1 (en) | 2020-01-27 | 2021-01-27 | Improved detection assays |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3168830A1 true CA3168830A1 (en) | 2021-08-05 |
Family
ID=74858746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3168830A Pending CA3168830A1 (en) | 2020-01-27 | 2021-01-27 | Improved detection assays |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20230183783A1 (https=) |
| EP (1) | EP4097250A1 (https=) |
| JP (1) | JP2023512985A (https=) |
| KR (1) | KR20220131939A (https=) |
| AU (1) | AU2021212731A1 (https=) |
| BR (1) | BR112022014777A2 (https=) |
| CA (1) | CA3168830A1 (https=) |
| IL (1) | IL295011A (https=) |
| MX (1) | MX2022009212A (https=) |
| TW (1) | TW202142698A (https=) |
| WO (1) | WO2021154866A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
| US12534743B2 (en) | 2018-08-31 | 2026-01-27 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| US12545910B2 (en) | 2018-08-31 | 2026-02-10 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| US20230235414A1 (en) * | 2020-05-27 | 2023-07-27 | King Abdullah University Of Science And Technology | Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2 |
| EP4274890A4 (en) * | 2021-01-07 | 2024-11-27 | Inscripta, Inc. | MAD-NUCLEASES |
| US20250277199A1 (en) | 2021-03-02 | 2025-09-04 | BRAIN Biotech AG | Novel crispr-cas nucleases from metagenomes |
| TW202321454A (zh) * | 2021-07-26 | 2023-06-01 | 美商夏洛克生物科學公司 | 經改良之ｃｒｉｓｐｒ－ｃａｓ技術 |
| EP4423263A4 (en) * | 2021-10-29 | 2026-03-04 | Mammoth Biosciences Inc | EFFECTOR PROTEINS, COMPOSITIONS, SYSTEMS, DEVICES, KITS AND METHODS FOR THERECOMMENDATION |
| WO2024005863A1 (en) * | 2022-06-30 | 2024-01-04 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| WO2024005864A1 (en) * | 2022-06-30 | 2024-01-04 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| US20260043073A1 (en) | 2022-07-01 | 2026-02-12 | Sherlock Biosciences, Inc. | Ambient temperature nucleic acid amplification and detection |
| WO2024218780A1 (en) * | 2023-04-20 | 2024-10-24 | Limay Bio Sa | Mehtods and systems for accelerated crispr-cas reaction-based detection of nucleic acids |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016100955A2 (en) * | 2014-12-20 | 2016-06-23 | Identifygenomics, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| WO2016205749A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
| CN110506128A (zh) | 2016-12-09 | 2019-11-26 | 博德研究所 | 基于crispr效应系统的诊断 |
| CN112501254B (zh) * | 2017-07-14 | 2024-07-19 | 上海吐露港生物科技有限公司 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
| US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
| CA3086550A1 (en) * | 2017-12-22 | 2019-06-27 | The Broad Institute, Inc. | Crispr effector system based multiplex diagnostics |
| WO2019178428A1 (en) * | 2018-03-14 | 2019-09-19 | Arbor Biotechnologies, Inc. | Novel crispr dna and rna targeting enzymes and systems |
| EP3931313A2 (en) * | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
| CN115175996A (zh) * | 2019-09-20 | 2022-10-11 | 博德研究所 | 新颖vi型crispr酶和系统 |
| US11453907B2 (en) * | 2020-03-23 | 2022-09-27 | The Broad Institute, Inc. | Crispr effector system based coronavirus diagnostics |
-
2021
- 2021-01-27 AU AU2021212731A patent/AU2021212731A1/en active Pending
- 2021-01-27 IL IL295011A patent/IL295011A/en unknown
- 2021-01-27 MX MX2022009212A patent/MX2022009212A/es unknown
- 2021-01-27 KR KR1020227027186A patent/KR20220131939A/ko active Pending
- 2021-01-27 JP JP2022545378A patent/JP2023512985A/ja active Pending
- 2021-01-27 WO PCT/US2021/015306 patent/WO2021154866A1/en not_active Ceased
- 2021-01-27 US US17/795,815 patent/US20230183783A1/en not_active Abandoned
- 2021-01-27 TW TW110103081A patent/TW202142698A/zh unknown
- 2021-01-27 EP EP21710084.1A patent/EP4097250A1/en active Pending
- 2021-01-27 BR BR112022014777A patent/BR112022014777A2/pt unknown
- 2021-01-27 CA CA3168830A patent/CA3168830A1/en active Pending
-
2022
- 2022-11-18 US US17/990,565 patent/US20230272458A1/en not_active Abandoned
-
2025
- 2025-03-14 US US19/080,542 patent/US20250327116A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20220131939A (ko) | 2022-09-29 |
| AU2021212731A1 (en) | 2022-08-25 |
| TW202142698A (zh) | 2021-11-16 |
| MX2022009212A (es) | 2022-11-09 |
| BR112022014777A2 (pt) | 2022-09-20 |
| JP2023512985A (ja) | 2023-03-30 |
| IL295011A (en) | 2022-09-01 |
| US20230272458A1 (en) | 2023-08-31 |
| US20250327116A1 (en) | 2025-10-23 |
| EP4097250A1 (en) | 2022-12-07 |
| US20230183783A1 (en) | 2023-06-15 |
| WO2021154866A1 (en) | 2021-08-05 |
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Legal Events
| Date | Code | Title | Description |
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| MFA | Maintenance fee for application paid |
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