JP2023113514A - Oligonucleotide for detecting omicron strain of sars-cov-2 - Google Patents

Abstract

To provide a method for detecting Omicron strain of SARS-CoV-2 from a sample possibly containing foreign matter without performing nucleic acid isolation and purification work in advance.SOLUTION: A method includes following steps: (1) mixing a sample not subjected to nucleic acid isolation, a primer set that includes (a) at least one primer including specific base sequences or complementary base sequences to these base sequences and (b) at least one primer designed to include complementary base sequences to a nucleic acid elongation product of the primer, and a RT-PCR reaction solution; and (2) performing a RT-PCR reaction using the liquid mixture.SELECTED DRAWING: None

Description

TECHNICAL FIELD The present invention relates to oligonucleotides capable of detecting SARS-CoV-2 Omicron strains by nucleic acid amplification, detection methods and detection kits using the oligonucleotides, and the like. The present invention enables highly sensitive detection of Omicron strains of SARS-CoV-2 from biological samples such as pharyngeal and nasal swabs, sputum and saliva without nucleic acid purification. The present invention can also be used for life science research, clinical diagnosis, food hygiene inspection, environmental inspection, and the like.

Nucleic acid amplification is a technology that amplifies a few copies of target nucleic acid to a level that can be visualized, that is, to hundreds of millions of copies or more. It is also widely used in microbiological examinations, etc. A typical nucleic acid amplification method is PCR (Polymerase Chain Reaction). PCR consists of (1) DNA denaturation by heat treatment (dissociation from double-stranded DNA to single-stranded DNA), (2) annealing of a primer to a template single-stranded DNA, and (3) conversion of the primer using a DNA polymerase. This is a method of amplifying a target nucleic acid in a sample by repeating three steps of elongation as one cycle. In some cases, annealing and extension are performed at the same temperature in two steps.

When analyzing RNA, reverse transcription (RT), which converts template RNA into cDNA, is performed as a preceding stage of this PCR. This is called RT-PCR. This RT-PCR consists of (1) 2-step RT-PCR in which RT and PCR are performed discontinuously, (2) RT and PCR in succession using a single enzyme 1-step RT -PCR, and (3) a two-enzyme one-step RT-PCR in which RT and PCR are successively performed using two enzymes, reverse transcriptase and DNA polymerase.

PCR and RT-PCR are also widely used for genetic testing and virus testing. Typical examples of RNA viruses targeted for such tests include pathogenic RNA viruses such as coronaviruses and influenza viruses.
Coronaviruses are causative viruses that cause respiratory infections including colds, and about 10 to 35% of colds are said to be caused by coronaviruses. Mutant viruses are also known to occur, and rarely SARS (Severe Acute Respiratory Syndrome) coronavirus, MERS (Middle East Respiratory Syndrome) coronavirus, novel coronavirus infectious disease (COVID-19) coronavirus (SARS) -nCOV-2, or SARS-CoV-2) are known to cause fatal serious respiratory diseases.

Since its outbreak in Wuhan, China in 2019, SAR-CoV-2 has spread around the world, becoming a pandemic. As the infection continues, more infectious strains and strains that are more likely to become severe are also occurring. From the perspective of preventing the spread of infection and diagnostic applications, simple, rapid, and highly sensitive detection and identification of variant strains (VOCs) that are concerned about increased infectivity and transmissibility and changes in antigenicity. is important. As SARS-CoV-2 mutant strains related to VOCs, various mutant strains such as alpha strain, beta strain, gamma strain, and delta strain have been discovered so far, and the highly infectious Omicron strain is currently a major problem. It has become. Therefore, from the viewpoint of preventing the spread of infection, it is desired to quickly identify the Omicron strain simply, quickly, and with high sensitivity.

A general SNP analysis method is usually used as a method for identifying viral mutations. For example, using PCR primers that can be amplified so as to sandwich the region containing the mutation and TaqMan probes complementary to the wild type and the mutant strain that are labeled with different fluorescence, these primers, TaqMan probes and viral RNA are mixed, Wild strains and mutant strains can be distinguished by performing RT-PCR. In addition, a method (ASP) in which primers are designed to amplify only mutant strains and the mutant strain is identified by the presence or absence of amplification, a fluorescent probe is reacted with the amplified product and the presence or absence of mutation is determined by melting curve analysis. methods, and a method of determining the presence or absence of mutations by melting curve analysis of amplification products using an intercalator dye such as SYBR (Non-Patent Document 1). These methods can also be combined, and a multiplex method for simultaneously detecting mutations at multiple sites has also been developed.

However, when identifying mutant strains using TaqMan probes and RT-PCR reactions, it is necessary to obtain different signals between wild strains and mutant strains, and optimization of reaction conditions is very important. That is, when identifying wild strains and mutant strains using different TaqMan probes as described above, it is necessary to change the fluorescence signal with a difference of only one or a few bases, and the selection of probe length, sequence, etc. Condition setting is important. Similarly, when using primers capable of amplifying only mutant strains, it is necessary to adjust the length, sequence, etc. of the primers so that they do not amplify wild-types that differ by only one or a few bases, which is not easy.

In genetic testing for respiratory infections, biological samples such as pharyngeal and nasal swabs, sputum, and saliva are used for genetic testing by RT-PCR. Biological samples contain many contaminants that inhibit the RT-PCR reaction and non-viral nucleic acids that cause non-specific amplification. Therefore, when the RT-PCR reaction is performed using such a biological sample containing contaminants as it is without isolating the nucleic acid, the reaction may be inhibited and the reaction may become undetectable due to poor amplification. As the system becomes more complicated, there is concern that non-specific reactions will occur and the detection sensitivity will decrease. Such a decrease in detection sensitivity can be a problem especially when it is necessary to determine wild strains and mutant strains by changing fluorescence signals with a difference of only one base, because it makes it difficult to distinguish between them.

Currently, from the viewpoint of preventing the spread of infection, there is a strong demand for a method for quickly and simply determining the Omicron strain of SARS-CoV-2. In particular, there has been a demand for the development of a useful method capable of performing RT-PCR reaction and determination without isolating nucleic acids from pharyngeal and nasal swabs, saliva, stool samples, swab environmental samples, and the like.

YAKUGAKU ZASSHI 122(7) 451-463 (2002)

The present invention has been made in view of the above prior art, and an object of the present invention is to provide a method for identifying and detecting the Omicron strain of SARS-CoV-2 simply and with high sensitivity. In addition, in order to make the test more rapid and simple, the present invention uses samples containing contaminants derived from biological samples such as saliva, pharyngeal swabs, and nasal swabs without nucleic acid isolation treatment. It is a further object to provide a method capable of identifying and detecting Omicron strains of SARS-CoV-2.

In view of the above circumstances, the present inventors have conducted intensive research, and as a result, identified and detected Omicron strains with high sensitivity by distinguishing them from wild strains, etc., by using primers containing specific sequences from among numerous candidate sequences. I found what I can do. In addition to these primers, nucleic acid amplification is performed with a primer set containing primers capable of amplifying other mutant strains such as wild strains and delta strains. It is possible to identify and detect the Omicron strain of SARS-CoV-2 by distinguishing it from wild strains and mutant strains such as delta strains, even when performing RT-PCR reactions without isolation and purification. I found it and arrived at the present invention.

The representative invention of the present application is as follows.
[Claim 1] A method for detecting an Omicron strain of SARS-CoV-2 without nucleic acid isolation treatment, the method comprising the following steps:
(1) a sample that has not undergone a nucleic acid isolation treatment, (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) the primer (2) mixing a primer set containing at least one primer designed to contain a nucleotide sequence complementary to the nucleic acid extension product of the RT-PCR reaction solution; and (2) using the mixture solution performing the RT-PCR reaction.
[Item 2] Item 1, wherein in the step (1), (c) a fluorescent probe containing the nucleotide sequence set forth in SEQ ID NO: 3 or 4 or a nucleotide sequence complementary thereto is further mixed. the method of.
[Item 3] The method according to item 1 or 2, wherein the (b) primer is a primer containing the nucleotide sequence set forth in SEQ ID NO: 7 or a nucleotide sequence complementary to the nucleotide sequence.
[Item 4] The method according to any one of Items 1 to 3, wherein the primer set further comprises a primer containing the nucleotide sequence set forth in SEQ ID NO: 6 or a nucleotide sequence complementary to the nucleotide sequence.
[Item 5] The fluorescent probe according to any one of Items 1 to 4, wherein in the step (1), a fluorescent probe containing the nucleotide sequence set forth in SEQ ID NO: 2 or a nucleotide sequence complementary to the nucleotide sequence is further mixed. Method.
[Item 6] The method according to Item 4 or 5, wherein the SARS-CoV-2 Omicron strain is detected by distinguishing it from the SARS-CoV-2 Delta strain.
[Item 7] The method according to any one of Items 1 to 6, wherein the sample is at least one selected from the group consisting of saliva, throat swab, nasal swab, sputum, and fecal specimen.
[Claim 8] A kit for detecting SARS-CoV-2 omicron strains without nucleic acid isolation treatment, the kit having the following features:
(1) (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) a nucleotide complementary to the nucleic acid extension product of the primer and (2) reaction reagents that allow RT-PCR reactions from samples that have not undergone nucleic acid isolation procedures.
[Item 9] The kit according to Item 8, further comprising (c) a fluorescent probe containing the nucleotide sequence set forth in SEQ ID NO: 3 or 4 or a nucleotide sequence complementary to those nucleotide sequences.
[Item 10] The kit according to Item 8 or 9, wherein the (b) primer is a primer containing the nucleotide sequence set forth in SEQ ID NO: 7 or a nucleotide sequence complementary to the nucleotide sequence.
[Item 11] The kit according to any one of Items 8 to 10, further comprising a primer comprising the nucleotide sequence set forth in SEQ ID NO: 6 or a nucleotide sequence complementary to the nucleotide sequence.
[Item 12] The kit according to any one of Items 8 to 11, further comprising a fluorescent probe comprising the nucleotide sequence set forth in SEQ ID NO: 2 or a nucleotide sequence complementary to the nucleotide sequence.
[Item 13] The kit according to Item 11 or 12, which is used for detecting the Omicron strain of SARS-CoV-2 by distinguishing it from the Delta strain of SARS-CoV-2.
[Item 14] The kit according to any one of Items 8 to 13, wherein the sample is at least one selected from the group consisting of saliva, throat swab, nasal swab, sputum, and fecal specimen.

INDUSTRIAL APPLICABILITY According to the present invention, SARS-CoV-2 Omicron strains can be identified and detected quickly and easily. In particular, according to the present invention, only by adding a biological sample such as saliva together with a primer set containing primers of specific sequences to the RT-PCR reaction solution and then performing the RT-PCR reaction, the Omicron strain of SARS-CoV-2 can be obtained. can be detected, there is no need to isolate nucleic acids from a biological sample in advance, which contributes to reducing the risk of contamination during nucleic acid isolation and shortening the examination time. Such efficiency improvement in examination work can shorten the time required for accurate treatment of a patient, and lead to rapid response to infection prevention, which can greatly contribute to the prevention of infectious diseases.

Furthermore, in the method of the present invention in which the RT-PCR reaction is performed using primers of specific sequences, the fluorescence label designed for wild-type SARS-CoV-2 detection is used as it is, and the SARS-CoV-2 Omicron strain can be distinguished from wild strains. Therefore, when detecting the Omicron strain of SARS-CoV-2, there is an advantage in that it is not necessary to design a plurality of fluorescently labeled probes for detecting the Omicron strain and for detecting the wild strain.

Hereinafter, the present invention will be described in further detail while showing embodiments of the present invention.

The present invention relates to a method for detecting SARS-CoV-2 Omicron strains without isolating nucleic acid, and a kit and the like for carrying out the method. The Omicron strain can contain a large number of mutations in the wild-type SARS-CoV-2 genome sequence (SEQ ID NO: 1), unlike other previously known mutant strains of SARS-CoV-2. Are known. Mutations in the Omicron strain of SARS-CoV-2 are known to be contained in many nucleotide sequences, particularly in the nucleotide sequence encoding the spike protein (also called S protein), for example, G142D, G339D, S371L, S373P, S375F, S477N. , T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H, and P681H. Viruses such as SARS-CoV-2 mutate easily, and it is presumed that Omicron strains will mutate repeatedly in the future. As used herein, the Omicron strain of SARS-CoV-2 refers to Aine O'Toole et al. (Virus Evolution, 2021, 7(2), 1-9, which is incorporated herein by reference in its entirety. using the Pangolin tool (Pangolin: Phylogenetic Assignment of Named Global Outbreak Lineages) described in B. 1. Refers to SARS-CoV-2 mutant strains classified as 1.1.529 (including, for example, BA.2 strain, etc.), and currently known Omicron strains (B.1.1.529 strain) Omicron strain derivatives having nucleotide sequences equivalent to (eg, nucleotide sequences having 99% or more identity in the region encoding the S protein of the Omicron strain). Such derivative strains of the Omicron strain include, but are not limited to, derivatives of the Omicron strain that include nucleotide sequences encoding S371L, S373P, S375F, S477N, T478K, and E484A in their genomic RNA.

In the present invention, in order to detect the Omicron strain of SARS-CoV-2, the region surrounding the L452 position in the S protein of SARS-CoV-2, specifically, the bases shown at 22875 to 22898 in SEQ ID NO: 1 One of the characteristics is that the SARS-CoV-2 Omicron strain is specifically detected by using primers designed to target regions corresponding to the sequences. In the primer used in the present invention, in the wild-type SARS-CoV-2 nucleotide sequence shown in SEQ ID NO: 1, T is replaced with G at the 22882nd base, and G is replaced with A at the 22898th base. . In the present invention, by performing an RT-PCR reaction using a primer set containing such primers, the Omicron strain of SARS-CoV-2 as described above can be detected separately from wild strains and the like.

In one embodiment, the present invention provides a method for detecting an omicron strain of SARS-CoV-2 without isolating nucleic acid, the method comprising at least the following steps (1) and (2): I will provide a:
(1) a sample that has not undergone a nucleic acid isolation treatment, (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) the primer A step of mixing a primer set containing at least one primer designed to contain a nucleotide sequence complementary to the nucleic acid extension product of and an RT-PCR reaction solution; and (2) using the mixture solution performing the RT-PCR reaction.
In the step (1) of the present invention, by using a primer set containing primers composed of specific nucleotide sequences, the SARS-CoV-2 Omicron strain can be highly discriminated and detected, and for example, saliva , Nasal swabs, even when biological samples containing contaminants such as pharyngeal swabs are used as they are, the SARS-CoV-2 Omicron strain that can be contained in these biological samples can be detected with high sensitivity. based on

In the above embodiment, one of the characteristics of the method of the present invention is to use a sample that has not undergone nucleic acid isolation treatment. For example, in the present invention, nucleic acids can be isolated from various samples using a commercially available nucleic acid purification kit, or untreated samples that have not undergone prior heat treatment or the like for exposing the viral genomic nucleic acid from the capsid structure can be used. can. It is preferable to use these untreated samples from the viewpoint of convenience because pretreatment such as laborious purification is unnecessary. Alternatively, it may be a sample that has undergone nucleic acid extraction without separation and purification to remove contaminants. Here, the sample subjected to nucleic acid extraction without separation and purification means that the nucleic acid is exposed in the sample. Extraction and exposure of encapsulated nucleic acids (however, cell membranes, capsids, envelope fragments, etc. remaining after disruption should not be removed). In the present invention, nucleic acids can be amplified satisfactorily even in a sample prepared without such isolation and purification, and the Omicron strain of SARS-CoV-2 can be stably detected. Such a nucleic acid extraction treatment without separation and purification can be performed prior to the step (1).

The sample used in the present invention is not particularly limited as long as it has not undergone nucleic acid isolation treatment. When the sample used in the present invention is a sample that may contain contaminants and insoluble substances such as saliva, nasal swabs, and pharyngeal swabs, the sample is prepared as a suspension that has been previously suspended in water, a buffer solution, or the like. A saliva sample or the like may be used as it is, or a solid sample such as a stool sample may be directly added to the PCR reaction solution. Examples include pharyngeal swabs, nasal swabs, nasopharyngeal swabs, sputum, vomit, saliva, gargle, tears, and feces (excretion, rectal stool), but are not particularly limited. It is possible to use it for all things derived from. In particular, it is useful for detection from pharyngeal swab, nasal swab, nasopharyngeal swab, sputum, lung aspirate, cerebrospinal fluid, gargle, saliva, tears, feces, cultured cells, and culture supernatant, Among others, it is useful for detection of saliva samples, sputum samples, gargles, tears, pharyngeal swabs, nasopharyngeal swabs, nasal swabs, and fecal samples. According to the present invention, there is an advantage that it is not necessary to isolate RNA from these samples using a commercially available RNA purification kit or the like and subject it to RT-PCR reaction. The sample may be directly subjected to detection, or a sample in which the sample is suspended in water, physiological saline, or a buffer solution in order to reduce the influence of contaminants on the reaction and obtain more stable test results. There may be. Examples of the buffer include, but are not limited to, Hank's buffer, Tris buffer, phosphate buffer, glycine buffer, HEPES buffer, and tricine buffer. In the case of a highly viscous biological sample (eg, a sample containing highly viscous sputum), the sample may be treated with a sputazyme enzyme solution, although not particularly limited.

Furthermore, the sample used in the present invention is mixed with a solution containing a buffer solution, an acid or alkaline solution, an organic solvent, etc., in order to facilitate the extraction of RNA or DNA from robust structures such as capsids and cell walls. It may be a sample that has been The acidic solution contained in the solution is not particularly limited as long as it is an acidic solution. Examples of acidic solutions include aqueous formic acid, aqueous acetic acid, aqueous butyric acid, aqueous hydrochloric acid, aqueous nitric acid, aqueous sulfuric acid, aqueous citric acid, aqueous lactic acid, aqueous phosphoric acid, aqueous benzoic acid, aqueous oxalic acid, aqueous tartaric acid, and aqueous ascorbic acid. , an aqueous sulfonic acid solution, etc., and can be used singly or in combination of two or more. The alkaline solution is not particularly limited as long as it is an alkaline solution. Examples of alkaline solutions include potassium hydroxide aqueous solution, sodium hydroxide aqueous solution, lithium hydroxide aqueous solution, magnesium hydroxide aqueous solution, calcium hydroxide aqueous solution, barium hydroxide aqueous solution, potassium carbonate aqueous solution, sodium carbonate aqueous solution, magnesium carbonate aqueous solution, calcium carbonate. Aqueous solutions, Tris buffers, glycine buffers, phosphate buffers, borate buffers, Good's buffers (TAPSO, POPSO, HEPSO, EPPS, Tricine, Bicine, TAPS, CHES, CAPS) and the like, one type alone Or it can be used in combination of two or more. Examples of organic solvents include ethanol, methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, triethylamine, dimethylformamide, hexamethylphosphorictriamide, dimethylsulfoxide, acetone, acetonitrile, ethanol, and methanol. , 1-propanol, 2-propanol, 1-butanol, pyridine and the like, but are not limited thereto. Furthermore, the sample used in the present invention may be subjected to heat treatment after being mixed with the solution. The conditions for the heat treatment are not particularly limited, but may be 60° C. or higher, preferably 70° C. or higher, more preferably 80° C. or higher, and still more preferably 90° C. or higher for 1 second or longer.

The method of detecting the Omicron strain of SARS-CoV-2 of the present invention uses a primer set comprising primers of specific sequences to amplify a portion of the genomic RNA region encoding the spike protein of the Omicron strain. The primer set used in the present invention comprises (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) the nucleic acid of the (a) primer. The primer set is not particularly limited as long as it includes at least one primer designed to contain a nucleotide sequence complementary to the extension product and is designed to allow nucleic acid amplification in RT-PCR reaction. In a preferred embodiment, the primer set used in the present invention is a primer set designed to amplify approximately 50 to 500 bp, and a primer set designed to amplify approximately 50 to 300 bp. More preferably, it is a primer set designed to amplify approximately 50 to 150 bp. As used herein, a primer containing a nucleotide sequence represented by a specific SEQ ID NO or a nucleotide sequence complementary to the nucleotide sequence refers to one or A few (eg, 1 to 3, preferably 1 to 2, more preferably 1) bases may be added. The addition of such bases can be appropriately designed by those skilled in the art according to the target sequence. In addition, the primer set used in the present invention may contain one each of (a) primer and (b) primer, or may contain two or more (a) primers and/or (b) two primers. may contain more than one.

In the primer set used in the present invention, (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence is a forward primer (also abbreviated as Fwd primer). It may be a reverse primer (also abbreviated as Rev primer). Preferably, the (a) primer is a forward primer and the (b) primer is a primer designed as a reverse primer. Preferably, the (b) primer designed as a reverse primer is a primer containing the nucleotide sequence set forth in SEQ ID NO: 7 or a nucleotide sequence complementary to the nucleotide sequence. Among them, the (a) primer consisting of the base sequence set forth in SEQ ID NO: 5 or a base sequence complementary to the base sequence and (b) the base sequence set forth in SEQ ID NO: 7 or the base sequence thereof as the primer and a primer consisting of a nucleotide sequence complementary to the (a) primer consisting of the nucleotide sequence set forth in SEQ ID NO: 5 as the (a) primer and (b) the primer set set forth in SEQ ID NO: 7 as the primer It is more preferable that the primer set contains a primer consisting of a nucleotide sequence of By performing an RT-PCR reaction using such a primer set, even when performing an RT-PCR reaction using a sample that has not undergone nucleic acid isolation treatment and using a reaction solution that may contain contaminants, Omicron strains can be amplified and detected with high sensitivity.

The primer set used in the present invention may contain primers other than the (a) primer and the (b) primer. Such primers can be, for example, primers designed to amplify the genomic RNA of the wild strain of SARS-CoV-2 or other SARS-CoV-2 mutant strains such as the delta strain. In addition to the (a) primer and the (b) primer, by using a primer set containing such primers in combination, not only the Omicron strain of SARS-CoV-2 but also other strains such as wild strains or delta strains It is also possible to detect SARS-CoV-2 mutant strains of SARS-CoV-2 at the same time, and whether SARS-CoV-2 contained in the biological sample is an Omicron strain or a strain other than that (for example, is it an Omicron strain? , whether it is a delta strain or a wild strain, etc.). Such a further primer is preferably a primer capable of amplifying the genomic RNA of a SARS-CoV-2 mutant strain other than the Omicron strain together with the primer (b), more preferably the (b) A primer capable of amplifying the genomic RNA of SARS-CoV-2 delta strain together with the primer, more preferably the nucleotide sequence set forth in SEQ ID NO: 6 or complementary to the nucleotide sequence thereof A primer containing a nucleotide sequence, more preferably a primer consisting of the nucleotide sequence set forth in SEQ ID NO: 6 or a nucleotide sequence complementary to the nucleotide sequence, and particularly preferably a primer comprising the nucleotide sequence set forth in SEQ ID NO: 6 Primer. The results of the test examples described later show that by using a combination of such primers, the Omicron strain and the Delta strain of SARS-CoV-2 can be distinguished and well distinguished using the same RT-PCR reaction solution. ing.

In one embodiment, in the present invention, using a fluorescence-labeled nucleic acid probe (hereinafter also referred to as (c) probe) designed to hybridize to a nucleic acid amplification product amplified with the primer set, SARS-CoV -2 Omicron strains are detected. Such fluorescent-labeled probes are not particularly limited as long as they generate a strong signal when the target nucleic acid amplification product is present. 5,538,848, U.S. Pat. No. 5,487,972, U.S. Pat. No. 5,804,375), molecular beacons (U.S. Pat. No. 5,118,801), FRET hybridization probes (International Publication No. 97/46707 pamphlet, International Publication No. 97/46712 pamphlet, International Publication No. 97/46714 pamphlet) and the like. Preferred are TaqMan probes.

Any fluorescent compound known in the art can be used as the fluorescent compound that can be used in the probe, and can be selected, for example, according to the qPCR equipment that one wishes to use. Specific examples of fluorescent compounds include rhodamine (ROX) or derivatives thereof (e.g., 5-carboxy-X-rhodamine, 6-carboxy-X-rhodamine, 5-carboxyrhodamine 6G (CR6G), tetramethylrhodamine (TAMRA )), or rhodamine compounds such as salts thereof; fluorescein or derivatives thereof (e.g., FAM (carboxyfluorescein), JOE (6-carboxy-4′,5′-dichloro2′,7′-dimethoxyfluorescein), FITC (fluorescein isothiocyanate), TET (tetrachlorofluorescein), HEX (5′-hexachloro-fluorescein-CE phosphoramidite)), VIC (registered trademark), BODIPY (registered trademark) series, rhodamine or derivatives thereof (for example, 5-carboxyrhodamine 6G (CR6G), tetramethylrhodamine (TAMRA)), Cy (registered trademark) dyes (e.g., Cy3, Cy5), derivatives thereof, and non-rhodamine compounds such as salts thereof. but not limited to these. A quenching substance suitable for the fluorescent substance to be used can be used as the fluorescent compound, if necessary. Examples of quenching substances corresponding to the above fluorescent substances include TAMRA (tetramethyl-rhodamine), DABCYL (4-(4-dimethylaminophenylazo)benzoic acid), BHQ1 (BHQ: Black Hole Quencher (registered trademark) )), BHQ2, BHQ3, and the like, but are not limited thereto.

The fluorescent probe capable of detecting the Omicron strain of SARS-CoV-2 as described above can hybridize to a part of the base sequence of either one of the double strands of the nucleic acid amplification product amplified with the primer set. The probe is not particularly limited as long as it is designed to be, for example, it may not be designed to contain a unique mutated base in the genome sequence of the Omicron strain of SARS-CoV-2, SARS- It may be a probe comprising a base sequence complementary to the CoV-2 wild-type genomic base sequence. Thus, a common base sequence between the SARS-CoV-2 Omicron strain and the wild type (preferably a continuous common base sequence of about 20 to 30 bp, more preferably a continuous base sequence of about 22 to 26 bp) By designing a fluorescent probe to target a common base sequence), it is not necessary to design multiple fluorescently labeled probes corresponding to each of the Omicron strains and wild strains, which reduces costs. It has the advantage of being able to detect Omicron strains of SARS-CoV-2. Examples of such fluorescent probes include, for example, fluorescent probes containing the nucleotide sequence of SEQ ID NO: 3 or 4 or complementary nucleotide sequences to those nucleotide sequences, preferably the nucleotide sequence of SEQ ID NO: 3 or its Examples include, but are not limited to, fluorescent probes containing a base sequence complementary to the base sequence, more preferably fluorescent probes consisting of the base sequence set forth in SEQ ID NO:3. As used herein, a probe containing a nucleotide sequence represented by a specific SEQ ID NO or a nucleotide sequence complementary to the nucleotide sequence refers to one or A few (eg, 1 to 3, preferably 1 to 2, more preferably 1) bases may be added. The addition of such bases can be appropriately designed by those skilled in the art according to the target sequence. In the present invention, one of the above fluorescent probes may be used, or two or more may be used in combination.

The primer set used in the present invention may contain a fluorescent probe other than the (c) probe. Examples of such fluorescent probes include fluorescent probes designed to detect nucleic acid amplification products from genomic RNA of other SARS-CoV-2 mutant strains such as SARS-CoV-2 delta strain. obtain. In addition to the (c) probe, by using such a fluorescent probe in combination, not only the Omicron strain of SARS-CoV-2 but also other SARS-CoV-2 mutant strains such as the delta strain are discriminated. There is an advantage that simultaneous detection is also possible. Such a further fluorescent probe is preferably a fluorescence capable of detecting a nucleic acid amplification product from the genomic RNA of the SARS-CoV-2 Delta strain, which is problematic due to its high infectivity as well as the Omicron strain. A probe, more preferably a fluorescent probe containing the base sequence set forth in SEQ ID NO: 2 or a base sequence complementary to the base sequence thereof, more preferably a base sequence set forth in SEQ ID NO: 2 or the base sequence thereof A fluorescent probe consisting of a base sequence complementary to , particularly preferably a fluorescent probe consisting of the base sequence set forth in SEQ ID NO:2. By using such fluorescent probes in combination, SARS-CoV-2 Omicron strain and Delta strain can be distinguished and well discriminated using the same RT-PCR reaction solution.

In the method of detecting the Omicron strain of SARS-CoV-2 of the present invention, even from a sample containing contaminants without nucleic acid isolation treatment, RT-PCR without inhibition of contaminants An RT-PCR reaction is performed using an RT-PCR reaction solution (RT-PCR reagents) in which the reaction can be performed. Such an RT-PCT reaction solution preferably contains, for example, a DNA polymerase known to exhibit resistance to contaminants, but is not particularly limited.

Any DNA polymerase known in the art can be used as the DNA polymerase contained in the RT-PCR reaction solution. Preferably, any DNA polymerase known in the art to be contaminant-resistant can be used. Contaminant resistance refers to the property of a DNA polymerase having high enzymatic activity sufficient for nucleic acid amplification reactions even in the presence of RT-PCR inhibitors. The contaminant-resistant DNA polymerase is not particularly limited, but includes Tth, Bst, KOD, Pfu, Pwo, Tbr, Tfi, Tfl, Tma, Tne, Vent, DEEP VENT, HawkZ05, and mutants thereof. Examples include, but are not limited to. Preference is given to using Tth and HawkZ05 or variants thereof. Particularly preferred is the use of Tth or variants thereof. Even DNA polymerases such as Taq, which normally do not have contamination resistance, can be used if they are mutants that have contamination resistance due to amino acid mutation. For example, the total amount of the contaminant-resistant DNA polymerase contained in the PCR reaction solution may be at least 4.2 ng/μL or more, preferably 5.0 ng/μL or more, and 5.8 ng/μL. It is more preferable to be above. Among them, it is preferably 8.3 ng/μL or more. The upper limit of the total amount of the contaminant-resistant DNA polymerase is not particularly limited, but as an example, it can be 20 ng/μL or less, and even if it is 16.7 ng/μL or less, the effect of the present invention can be sufficiently obtained. can be done. The amount of polymerase is a value quantified by the Bradford method or Nanodrop (Thermo Fisher), and may be estimated from the safety data sheet (SDS). When protein such as BSA is included, it is desirable to calculate by the latter method. In order to enhance the effect of suppressing non-specific reactions, enzymatic activity of DNA polymerase is suppressed until the start of PCR reaction by using it in combination with an anti-DNA polymerase antibody or introducing a thermolabile blocking group into DNA polymerase by chemical modification. , preferably applicable to hot-start RT-PCR.

The RT-PCR reaction solution used in the present invention contains DNA polymerase and, if necessary, reverse transcriptase. The origin of the reverse transcriptase contained in the RT-PCR reaction solution is not particularly limited as long as it can convert RNA into DNA. , RAV2-RT, EIAV-RT, Carboxydothermus hydrogenoformam DNA polymerase) and variants thereof. Particularly preferred examples include MMLV-RT, AMV-RT, or variants thereof.

The RT-PCR reaction solution used in the present invention may contain a DNA polymerase that also has reverse transcriptase activity. A DNA polymerase with reverse transcription activity is a DNA polymerase that has both the ability to convert RNA into cDNA and the ability to amplify DNA. A DNA polymerase having reverse transcription activity preferably has heat resistance in addition to reverse transcription activity and contaminant resistance. The term "heat resistance" means that the enzymatic activity does not decrease by more than half even after heat treatment at 70°C for 1 minute or more. Although the origin is not particularly limited, Taq, Tth, Bst, Bca, KOD, Pfu, Pwo, Tbr, Tfi, Tfl, Tma, Tne, Vent, DEEPVENT and variants thereof can be mentioned. As DNA polymerases having reverse transcription activity, thermus aquaticus-derived DNA polymerase (Taq), Thermus thermophilus HB8-derived DNA polymerase (Tth), Thermus sp Z05-derived DNA polymerase (Z05), and Thermotoga maritima-derived DNA polymerase have been used. (Tma), Bacillus caldotenax-derived DNA polymerase (Bca), Bacillus stearothermophilus-derived DNA polymerase (Bst), etc., and even mutants that do not lose their reverse transcription activity and thermostable DNA polymerase activity. good. In addition, mutants of DNA polymerase (KOD) derived from Thermococcus kodakaraensis and having reverse transcription activity are known (e.g., RTX: reverse transcription xenopolymerase), and the present invention includes such a reverse transcriptase activity A thermostable DNA polymerase that also has Especially preferred are DNA polymerases having reverse transcription activity selected from the group consisting of Tth, Z05 and variants thereof.

As used herein, a DNA polymerase mutant having contaminant resistance refers to, for example, 85% or more, preferably 90% or more, more preferably 95% or more of the amino acid sequence of the wild-type DNA polymerase from which it is derived. Further preferably, it has a sequence identity of 98% or more, particularly preferably 99% or more, and has a high DNA polymerase activity even in the presence of contaminants. In the case of a DNA polymerase that also has a reverse transcription activity, it has the activity of converting RNA into cDNA and the activity of amplifying DNA even in the presence of contaminants. Here, any method known in the art can be used to calculate the identity of amino acid sequences. For example, it can be calculated using a commercially available analysis tool or available through an electric communication line (Internet), for example, the homology algorithm BLAST (Basic local alignment search tool) http of the National Center for Biotechnology Information (NCBI) ://www. ncbi. nlm. nih. Amino acid sequence identity can be calculated by using default parameters in gov/BLAST/. Mutants that can be used in the present invention have one or several amino acid substitutions, deletions, insertions and/or additions (hereinafter collectively referred to as " It may be a polypeptide consisting of an amino acid sequence that has been mutated (also referred to as "mutated"), and have the same activities as wild-type DNA polymerase to convert RNA into cDNA and to amplify DNA. Here, one or several may be, for example, 1 to 80, preferably 1 to 40, more preferably 1 to 10, still more preferably 1 to 5, but is not particularly limited.

The RT-PCR reaction solution used in the present invention may contain, in addition to the DNA polymerase, buffers, suitable salts, magnesium salts or manganese salts, deoxynucleotide triphosphates, and additives as necessary. .

Buffers used in the present invention include, but are not limited to, Tris, Tricine, Bis-Tricine, Bicine, and the like. It is adjusted to pH 6-9, more preferably pH 7-9, with sulfuric acid, hydrochloric acid, acetic acid, phosphoric acid, or the like. Moreover, the concentration of the added buffer is 10 to 200 mM, preferably 20 to 150 mM. At this time, a salt solution is added to provide suitable ionic conditions for the reaction. Salt solutions include potassium chloride, potassium acetate, potassium sulfate, ammonium sulfate, ammonium chloride, ammonium acetate, and the like.

As dNTPs used in the present invention, dATP, dCTP, dGTP and dTTP are each added at 0.1 to 0.5 mM, most generally about 0.2 mM. Precautions against cross-contamination may be taken by using dUTP instead of and/or as part of dTTP. Inclusion of Uracil-N-glycosylase (UNG) is preferred when taking precautions against cross-contamination.

Furthermore, in the present invention, the RT-PCR reaction solution preferably contains divalent cations. Containing divalent cations in this way makes it possible to obtain more stable and high contamination resistance and to perform highly sensitive detection. Examples of divalent cations include, but are not limited to, magnesium ions, manganese ions, calcium ions, copper ions, iron ions, nickel ions, zinc ions, and the like. Preferably, magnesium ions and manganese ions are included as divalent cations. In the present invention, when magnesium ions, manganese ions, or the like are added to the PCR reaction solution, magnesium or manganese may be added, or salts thereof may be added. Examples of magnesium or salts thereof include magnesium, magnesium chloride, magnesium sulfate, magnesium acetate, etc. Examples of manganese or salts thereof include manganese, manganese chloride, manganese sulfate, manganese acetate, and the like. Magnesium, manganese, or a salt thereof is preferably added to the RT-PCR reaction solution in an amount of about 1 to 10 mM. In the detection method of the present invention, manganese or a salt thereof is preferably contained from the viewpoint that high sensitivity can be obtained stably when single-enzyme RT-PCR is performed. In a specific embodiment, the RT-PCR reaction solution preferably contains 1 mM or more manganese or a salt thereof, preferably 1.5 mM or more manganese or a salt thereof, and 2.0 mM or more manganese or a salt thereof. It is more preferable to include Moreover, when performing a two-enzyme system RT-PCR, it is preferable to contain magnesium or a salt thereof from the viewpoint of easily obtaining high sensitivity stably. In a specific embodiment, the RT-PCR reaction solution preferably contains 1 mM or more magnesium or a salt thereof, preferably 1.5 mM or more magnesium or a salt thereof, and 2.0 mM or more magnesium or a salt thereof. It is more preferable to include

Furthermore, as additives contained in the RT-PCR reaction solution, a quaternary ammonium salt having a structure in which three methyl groups are added to the amino group of an amino acid (hereinafter referred to as "betaine-like quaternary ammonium"), a polypeptide ( It may contain at least one selected from the group consisting of bovine serum albumin, sericin, blocking peptide fragment (hereinafter also referred to as BPF), gelatin), glycerol, glycol and surfactants.

The polypeptide used in the present invention is not particularly limited as long as it has a molecular weight of 5-500 kDa, preferably 6-400 kDa. In this specification, when molecular weights are indicated, they refer to values determined using SDS-PAGE, unless clearly indicated otherwise. The measurement of molecular weight by SDS-PAGE can be carried out using commercially available molecular weight markers and the like using techniques and devices commonly used in the field. For example, a "molecular weight of 50 kDa" refers to a range in which a person skilled in the art normally determines that there is a band at the position of 50 kDa when the molecular weight is measured by SDS-PAGE. Also, the polypeptide used in the present invention may be a mixture of polypeptides within the above molecular weight range.

The polypeptide used in the present invention is not particularly limited as long as the effect of the present invention is exhibited, and refers to a protein formed by connecting a plurality of amino acids via peptide bonds. In addition, the polypeptide used in the present invention may be, for example, a heat-denatured polypeptide (eg, gelatin) whose three-dimensional structure is unfolded by heat denaturation or the like, as long as it has a polypeptide structure in which amino acids are linked. may Specifically, polypeptides that can be used in the present invention include, for example, albumin (eg, bovine serum albumin, lactalbumin, human serum albumin, egg-derived albumin), gelatin (eg, fish gelatin, porcine gelatin), sericin, Naturally derived proteins (naturally derived polypeptides) such as casein and fibroin; blocking peptide fragments (hereinafter also referred to as BPF), collagen hydrolysates, polypeptones, yeast extracts, beef extracts, etc. artificially produced by synthesis/decomposition Polypeptides and the like can be used. From the viewpoint of exhibiting even better effects of the present invention, the polypeptide used in the present invention is preferably bovine serum albumin, gelatin, blocking peptide fragment (hereinafter BPF), and/or sericin. Bovine serum albumin and gelatin (especially fish gelatin) are more preferably used from the viewpoint that a high effect can be exhibited even in a small amount. These polypeptides may be used alone or in combination of two or more. In addition, these polypeptides may be prepared by means of extraction from nature, synthesis, or the like, and commercially available products can also be suitably used.

In the present invention, the amount of the polypeptide used is not particularly limited as long as the effect of the present invention is achieved. It can be used in an amount of 0.0001 to 200 mg/mL, preferably 0.01 to 150 mg/mL, more preferably 0.1 to 130 mg/mL, even more preferably 0.5 to 100 mg/mL. . A preferred amount for exhibiting a better effect may vary depending on the type of polypeptide used, the degree of desired effect, and the like. For example, the following amounts can be used.
- When bovine serum albumin is used: final concentration in the RT-PCR reaction solution is, for example, 0.5 mg/mL or higher, preferably 1 mg/mL or higher, more preferably 2 mg/mL or higher, and still more preferably 3 mg/mL or higher. Although the upper limit is not particularly limited, it can be, for example, 10 mg/mL or less.
- When using gelatin: final concentration in the RT-PCR reaction solution is, for example, 0.1 mg/mL or more, preferably 1 mg/mL or more, more preferably 5 mg/mL or more, still more preferably 7.5 mg/mL or more, and More preferably 15 mg/mL or more. Although the upper limit is not particularly limited, it can be, for example, 50 mg/mL or less.
- When using sericin: final concentration in the RT-PCR reaction solution is, for example, 1 mg/mL or more, preferably 5 mg/mL or more, more preferably 10 mg/mL or more, still more preferably 20 mg/mL or more, still more preferably 50 mg / mL or more. Although the upper limit is not particularly limited, it can be, for example, 100 mg/mL or less.
- When using BPF: final concentration in the RT-PCR reaction solution is, for example, 1 mg/mL or more, preferably 5 mg/mL or more, more preferably 10 mg/mL or more, still more preferably 20 mg/mL or more, and even more preferably 30 mg / mL or more. Although the upper limit is not particularly limited, it can be, for example, 50 mg/mL or less.

Surfactants contained in the RT-PCR reaction solution include Triton X-100, Triton X-114, Tween20, Nonidet P40, Briji35, Briji58, SDS, CHAPS, CHAPSO, Emulgen420, etc., but not particularly limited. Although the concentration of the surfactant in the RT-PCR reaction solution is not particularly limited, it is preferably 0.0001% or more, more preferably 0.002% or more, and still more preferably 0.005% or more, and good detection can be achieved. becomes possible. Although the upper limit is not particularly limited, it can be 0.1% or less as an example.

Examples of the betaine-like quaternary ammonium contained in the RT-PCR reaction solution include betaine (trimethylglycine) and carnitine, but are not particularly limited. The betaine structure is a stable compound with both positive and negative charges in the molecule, and it is thought to exhibit surfactant-like properties and cause destabilization of the virus structure. In addition, it is known to facilitate nucleic acid amplification of DNA polymerases. A preferred concentration of said betaine-like quaternary ammonium is 0.1M to 2M, more preferably 0.2M to 1.2M.

Furthermore, it can be used in combination with substances known in the art to promote PCR or RT-PCR. Facilitators useful in the present invention include, for example, glycerol, polyols, protease inhibitors, single-strand binding protein (SSB), T4 gene 32 protein, tRNA, sulfur or acetic acid containing compounds, dimethylsulfoxide (DMSO), glycerol, ethylene Glycol, propylene glycol, trimethylene glycol, formamide, acetamide, ectoine, trehalose, dextran, polyvinylpyrrolidone (PVP), tetramethylammonium chloride (TMAC), tetramethylammonium hydroxide (TMAH), tetramethylammonium acetate (TMAA), Examples include, but are not limited to, polyethylene glycol. In order to further reduce reaction inhibition, ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), 1,2-bis(o-aminophenoxy)ethane- A chelating agent such as N,N,N',N'-tetraacetic acid (BAPTA) may be included.

In addition, an RT-PCR reaction solution (RT-PCR reagent ), commercially available products can also be used, for example, SARS-CoV-2 Detection Kit (Toyobo), SARS-CoV-2 Direct Detection RT-qPCR Kit (TaKaRa), 2019 novel coronavirus detection reagent kit (Shimadzu ) can be used. SARS-CoV-2 Detection Kit (Toyobo) is preferably used.

The cycle of the RT-PCR reaction in the step (2) includes: 1. 2. heat treatment; A reverse transcription reaction step may be included. Moreover, before and after each step, a heat treatment step for activating the hot start enzyme may be included. One heat treatment step can include disrupting the virus to expose the nucleic acid within the virus and/or activating hot start enzymes in a nucleic acid amplification reaction. The temperature and time of the heat treatment step may be 60° C. or higher and 1 second or longer, preferably 70° C. for 30 seconds or longer, more preferably 80° C. for 30 seconds or longer, particularly preferably 85° C. for 30 seconds or longer. seconds or more. The temperature of the reverse transcription reaction in 2 is determined by the reverse transcription activity of the reverse transcriptase used and the Tm values of the primers and probes, and may be at least 25°C or higher. More preferably, it is 37°C or higher. In PCR, [1] DNA denaturation by heat treatment (dissociation from double-stranded DNA to single-stranded DNA), [2] annealing of a primer to a template single-stranded DNA, [3] conversion of the primer using a DNA polymerase elongation, 3 steps may be included, and [2] and [3] may be performed at the same temperature to form 2 steps. In order to perform rapid RT-PCR, the thermal cycler used for the RT-PCR reaction has a total elongation time of steps [2] and [3] of 15 seconds or less, more preferably 10 seconds or less. It is desirable to set up a measurement program for As used herein, the term "PCR elongation time" refers to the time set in the thermal cycler.

Another aspect of the present invention is a detection kit for the Omicron strain of SARS-CoV-2, which is characterized by comprising a primer with a specific nucleotide sequence as described above. Specifically, the kit of the present invention includes (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) the nucleic acid of the primer. and at least one primer designed to contain a nucleotide sequence complementary to the extension product, more preferably (b) as a primer, the nucleotide sequence set forth in SEQ ID NO: 7 or complementary to the nucleotide sequence thereof It may contain a primer containing a specific base sequence. This kit preferably further contains a reaction reagent that allows RT-PCR reaction without being inhibited by contaminants even from a sample containing contaminants without undergoing nucleic acid isolation treatment. In addition, these kits preferably contain the (c) fluorescent probe capable of detecting the Omicron strain of SARS-CoV-2. In addition, other primers capable of amplifying genomic RNA of other mutant strains such as SARS-CoV-2 delta strain and/or amplification products from genomic RNA of other mutant strains such as SARS-CoV-2 delta strain It is further preferred to include other fluorescently labeled probes capable of detecting The primers, fluorescent probes, and other components that may be included in the kit of the present invention may be the same as those detailed in the mutation detection method described above. In the kit of the present invention, the primers (primer set) and fluorescent probes for detecting the Omicron strain of SARS-CoV-2 as described above, and reaction reagents capable of RT-PCR reaction, and if necessary, Fluorescent probes, primers (primer sets), etc. for detecting other mutant strains of SARS-CoV-2 other than the Omicron strain (for example, Delta strain) are included in the kit as one reagent. Alternatively, they may be provided as separate reagents contained in a kit and mixed prior to use to be prepared.

EXAMPLES The present invention will be specifically described below with reference to examples. However, the present invention is not limited to the following examples.

Test example 1. Separate detection of SARS-CoV-2 Omicron strain SARS-CoV-2 Detection Kit -Multi- (Toyobo) using the pretreatment solution, reaction solution, and enzyme solution attached to it, having a specific base sequence in the presence of saliva It was evaluated whether SARS-CoV-2 Omicron strain can be detected separately from strains other than Omicron (wild strain, delta strain) by using primers. Specifically, 8 μL of a negative saliva sample is added to 3 μL of the pretreatment solution of the kit, and after reacting for 5 minutes at room temperature and 5 minutes at 95° C., 30 μL of the reaction solution and 5 μL of the enzyme solution, 10 μM of the Fwd primer described in SEQ ID NO: 5 1.2 μL and/or 10 μM Fwd primer set forth in SEQ ID NO: 6, 10 μM Rev primer set forth in SEQ ID NO: 7, 1.2 μL, 10 μM FAM-BHQ3 labeled probe (SARS- 0.3 μL of either a fluorescent probe designed based on the wild-type base sequence of CoV-2) or 10 μM of SARS-CoV-2 delta strain detection Cy5-BHQ3-labeled probe described in SEQ ID NO: 2, and sterilized water. An RT-PCR reaction solution was prepared so that the final volume was 40 μL. 1 μL of SARS-CoV-2 Omicron strain RNA, Delta strain RNA, and wild strain RNA (10000, 50, 25, 0 copies/μL, respectively) were added thereto, and RT-PCR reaction was performed. Since the RT-PCR reaction solution prepared here was not subjected to an operation to remove contaminants derived from saliva, it can be regarded as a pseudo-sample without nucleic acid isolation treatment. RT-PCR was performed under the conditions of 45 cycles of 5 minutes at 42°C, 10 seconds at 95°C, and then 5 seconds at 95°C → 30 seconds at 60°C.

Table 1 shows the Ct values obtained as a result of RT-PCR. N/A indicates no amplification. With the primer set of the combination of SEQ ID NOs: 6-7, signals were obtained only for the wild strain and the delta strain, and the wild strain and the Omicron strain were successfully separated, but the Omicron strain could not be detected. In the combination of primers of SEQ ID NOs: 5-7, the result was that it also reacted with the wild type when the copy number was high. The result was that the reaction was more efficient than the wild strain. In addition, in the combination of SEQ ID NOS: 5-6-7, wild strains and Omicron strains can be detected without omission when fluorescent probes designed based on FAM-labeled wild-type sequences are used, and Cy5-labeled It was shown that the simultaneous isolation of the delta strain and the wild type/omicron strain is possible by also using a probe designed based on the sequence of the delta strain.

From this result, it can be seen that the use of the primer set of SEQ ID NOS: 5-7 enables efficient amplification and detection of only the Omicron strain. In addition, by simultaneously performing detection by RT-PCR reaction using the primer set of SEQ ID NO: 6-7 and / or the primer set of SEQ ID NO: 5-6-7, not only the Omicron strain can be detected, but also the wild strain and / Or it became clear that the delta strain and the omicron strain could be separated and detected.

INDUSTRIAL APPLICABILITY The present invention can be suitably used in clinical examinations, examinations aimed at preventing the spread of infectious diseases, and in molecular biological research.

Claims (14)

A method for detecting Omicron strains of SARS-CoV-2 without nucleic acid isolation, the method comprising the steps of:
(1) a sample that has not undergone a nucleic acid isolation treatment, (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) the primer (2) mixing a primer set containing at least one primer designed to contain a nucleotide sequence complementary to the nucleic acid extension product of the RT-PCR reaction solution; and (2) using the mixture solution performing the RT-PCR reaction. 2. The method according to claim 1, wherein in step (1), (c) a fluorescent probe containing the base sequence of SEQ ID NO: 3 or 4 or a base sequence complementary to those base sequences is further mixed. 3. The method according to claim 1 or 2, wherein the (b) primer is a primer comprising the nucleotide sequence set forth in SEQ ID NO: 7 or a nucleotide sequence complementary to the nucleotide sequence. 4. The method according to any one of claims 1 to 3, wherein the primer set further comprises a primer comprising the nucleotide sequence set forth in SEQ ID NO: 6 or a nucleotide sequence complementary to the nucleotide sequence. 5. The method according to any one of claims 1 to 4, wherein in step (1), a fluorescent probe containing the base sequence of SEQ ID NO: 2 or a base sequence complementary to the base sequence is further mixed. 6. The method according to claim 4 or 5, wherein the Omicron strain of SARS-CoV-2 is detected by distinguishing it from the Delta strain of SARS-CoV-2. The method according to any one of claims 1 to 6, wherein the sample is at least one selected from the group consisting of saliva, throat swab, nasal swab, sputum, and fecal specimen. A kit for the detection of SARS-CoV-2 Omicron strains without nucleic acid isolation, the kit having the following features:
(1) (a) at least one primer containing the nucleotide sequence set forth in SEQ ID NO: 5 or a nucleotide sequence complementary to the nucleotide sequence, and (b) a nucleotide complementary to the nucleic acid extension product of the primer and (2) reaction reagents that allow RT-PCR reactions from samples that have not undergone nucleic acid isolation procedures. 9. The kit according to claim 8, further comprising (c) a fluorescent probe comprising the base sequence of SEQ ID NO: 3 or 4 or a base sequence complementary to those base sequences. 10. The kit according to claim 8 or 9, wherein the (b) primer is a primer comprising the nucleotide sequence of SEQ ID NO: 7 or a nucleotide sequence complementary to the nucleotide sequence. 11. The kit according to any one of claims 8 to 10, further comprising a primer comprising the base sequence set forth in SEQ ID NO: 6 or a base sequence complementary to the base sequence. 12. The kit according to any one of claims 8 to 11, further comprising a fluorescent probe containing the base sequence set forth in SEQ ID NO: 2 or a base sequence complementary to the base sequence. 13. The kit according to claim 11 or 12, which is used to distinguish and detect the Omicron strain of SARS-CoV-2 from the Delta strain of SARS-CoV-2. The kit according to any one of claims 8 to 13, wherein the sample is at least one selected from the group consisting of saliva, throat swab, nasal swab, sputum, and fecal specimen.