JP2023084119A - 複数の蛍光体の混合蛍光応答を解析する方法、蛍光解析器、蛍光顕微鏡およびコンピュータプログラム - Google Patents

複数の蛍光体の混合蛍光応答を解析する方法、蛍光解析器、蛍光顕微鏡およびコンピュータプログラム Download PDF

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JP2023084119A
JP2023084119A JP2022194013A JP2022194013A JP2023084119A JP 2023084119 A JP2023084119 A JP 2023084119A JP 2022194013 A JP2022194013 A JP 2022194013A JP 2022194013 A JP2022194013 A JP 2022194013A JP 2023084119 A JP2023084119 A JP 2023084119A
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reference emission
emission spectrum
fluorescence
sample
fluorophores
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JP2023084119A5 (https=
Inventor
ダイスラー ベンヤミン
Deissler Benjamin
シューマン クリスティアン
schumann Christian
リッチェル カイ
Ritschel Kai
ベルジャン メイト
Beljan Mate
フーケ ヴェアナー
Fouquet Wernher
コジョク ゲオルゲ
Cojoc Gheorghe
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Leica Microsystems CMS GmbH
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Leica Microsystems CMS GmbH
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Publication of JP2023084119A publication Critical patent/JP2023084119A/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/127—Calibration; base line adjustment; drift compensation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2022194013A 2021-12-06 2022-12-05 複数の蛍光体の混合蛍光応答を解析する方法、蛍光解析器、蛍光顕微鏡およびコンピュータプログラム Pending JP2023084119A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21212550 2021-12-06
EP21212550.4A EP4191234B1 (en) 2021-12-06 2021-12-06 Method of analysing a mixed fluorescence response of a plurality of fluorophores, fluorescence analyser, fluorescence microscope and computer program

Publications (2)

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JP2023084119A true JP2023084119A (ja) 2023-06-16
JP2023084119A5 JP2023084119A5 (https=) 2025-12-12

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Country Link
US (1) US12339229B2 (https=)
EP (1) EP4191234B1 (https=)
JP (1) JP2023084119A (https=)
CN (1) CN116223457A (https=)

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FR3117595B1 (fr) * 2020-12-16 2023-10-06 Commissariat Energie Atomique Dispositif compact de caractérisation d’une substance photoluminescente
US20240273678A1 (en) * 2023-01-30 2024-08-15 The Research Foundation For The State University Of New York Unmixing image data
CN116879250A (zh) * 2023-07-07 2023-10-13 宁波力显智能科技有限公司 荧光显微成像方法、装置、计算机设备和存储介质

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US6687000B1 (en) * 2000-06-26 2004-02-03 Wisconsin Alumni Research Foundation Photon-sorting spectroscopic microscope system
DE10222779A1 (de) * 2002-05-16 2004-03-04 Carl Zeiss Jena Gmbh Verfahren und Anordnung zur Untersuchung von Proben
DE10222359B4 (de) * 2002-05-21 2005-01-05 Max Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur spektral differenzierenden, bildgebenden Messung von Fluoreszenzlicht
CA2920492C (en) * 2013-09-30 2019-08-13 Ventana Medical Systems, Inc. Systems and methods for adaptive histopathology image unmixing
DE102018124129A1 (de) 2017-12-04 2019-06-06 Leica Microsystems Cms Gmbh Mikroskopsystem und Verfahren zur mikroskopischen Abbildung mit einem solchen Mikroskopsystem
US11703454B2 (en) * 2020-01-03 2023-07-18 Korea Advanced Institute Of Science And Technology Method and apparatus for multiplexed imaging of spectrally-similar fluorophores
DE102020107762A1 (de) 2020-03-20 2021-09-23 Leica Microsystems Cms Gmbh Fluoreszenzmikroskop und Verfahren zur Abbildung eines Objekts

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US12339229B2 (en) 2025-06-24
CN116223457A (zh) 2023-06-06
EP4191234B1 (en) 2025-02-19
US20230175963A1 (en) 2023-06-08
EP4191234A1 (en) 2023-06-07

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