JP2022532760A - インテグリンアゴニストを使用する改善された培養法 - Google Patents

インテグリンアゴニストを使用する改善された培養法 Download PDF

Info

Publication number
JP2022532760A
JP2022532760A JP2021568505A JP2021568505A JP2022532760A JP 2022532760 A JP2022532760 A JP 2022532760A JP 2021568505 A JP2021568505 A JP 2021568505A JP 2021568505 A JP2021568505 A JP 2021568505A JP 2022532760 A JP2022532760 A JP 2022532760A
Authority
JP
Japan
Prior art keywords
integrin
cells
antibody
epithelial stem
agonist
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2021568505A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022532760A5 (https=
JPWO2020234250A5 (https=
Inventor
ラウ ウィリブロルダス バレンド マリア ド
ヨハネス カロルス クレバーズ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Nederlandse Akademie van Wetenschappen
Original Assignee
Koninklijke Nederlandse Akademie van Wetenschappen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Nederlandse Akademie van Wetenschappen filed Critical Koninklijke Nederlandse Akademie van Wetenschappen
Publication of JP2022532760A publication Critical patent/JP2022532760A/ja
Publication of JP2022532760A5 publication Critical patent/JP2022532760A5/ja
Publication of JPWO2020234250A5 publication Critical patent/JPWO2020234250A5/ja
Priority to JP2025039830A priority Critical patent/JP2025090761A/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2842—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta1-subunit-containing molecules, e.g. CD29, CD49
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C12N5/0689—Stem cells; Progenitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/585—Integrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Immunology (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021568505A 2019-05-17 2020-05-18 インテグリンアゴニストを使用する改善された培養法 Pending JP2022532760A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025039830A JP2025090761A (ja) 2019-05-17 2025-03-13 インテグリンアゴニストを使用する改善された培養法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1906978.0 2019-05-17
GBGB1906978.0A GB201906978D0 (en) 2019-05-17 2019-05-17 Improved culture method using integrin agonist
PCT/EP2020/063855 WO2020234250A1 (en) 2019-05-17 2020-05-18 Improved culture method using integrin agonist

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025039830A Division JP2025090761A (ja) 2019-05-17 2025-03-13 インテグリンアゴニストを使用する改善された培養法

Publications (3)

Publication Number Publication Date
JP2022532760A true JP2022532760A (ja) 2022-07-19
JP2022532760A5 JP2022532760A5 (https=) 2023-05-17
JPWO2020234250A5 JPWO2020234250A5 (https=) 2023-05-17

Family

ID=67385087

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021568505A Pending JP2022532760A (ja) 2019-05-17 2020-05-18 インテグリンアゴニストを使用する改善された培養法
JP2025039830A Pending JP2025090761A (ja) 2019-05-17 2025-03-13 インテグリンアゴニストを使用する改善された培養法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025039830A Pending JP2025090761A (ja) 2019-05-17 2025-03-13 インテグリンアゴニストを使用する改善された培養法

Country Status (11)

Country Link
US (1) US20220340879A1 (https=)
EP (1) EP3987005A1 (https=)
JP (2) JP2022532760A (https=)
KR (1) KR20220024075A (https=)
CN (1) CN114144516A (https=)
AU (1) AU2020277651A1 (https=)
CA (1) CA3140269A1 (https=)
GB (1) GB201906978D0 (https=)
IL (1) IL288046A (https=)
SG (1) SG11202112547SA (https=)
WO (1) WO2020234250A1 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015261380B2 (en) 2014-05-16 2021-04-15 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
GB201721615D0 (en) 2017-12-21 2018-02-07 Koninklijke Nederlandse Akademie Van Wetenschappen Immune cell organoid co-cultures
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
WO2021081663A1 (en) * 2019-10-31 2021-05-06 University Health Network Medium and methods for culturing organoids
US20230029554A1 (en) * 2019-12-12 2023-02-02 The Walter And Eliza Hall Institute Of Medecal Research Organoid cultures
CN113493763A (zh) * 2020-03-18 2021-10-12 四川大学华西医院 可应用于基因编辑的小鼠卵巢原代细胞培养基及体外培养方法
CN111534477B (zh) * 2020-05-13 2021-12-21 江苏省人民医院（南京医科大学第一附属医院） 小鼠肺组织原代上皮干细胞球培养方法
EP4182442A4 (en) * 2020-07-17 2024-11-13 The Board of Trustees of the Leland Stanford Junior University METHODS FOR INFECTION OF HUMAN DISTAL LUNG ORGANOIDS WITH SARS-COV-2 AND OTHER PATHOGENS AND THEIR DIFFERENTIATION
CN112961819B (zh) * 2020-12-01 2023-06-09 保信亚太生物科技（深圳）有限公司 博卡病毒小肠上皮类器官感染模型的构建方法
WO2022261258A1 (en) * 2021-06-08 2022-12-15 Carnegie Mellon University Methods for fabricating airway organoids with native-like, apical-out polarity
GB202109913D0 (en) 2021-07-09 2021-08-25 Stichting Hubrecht Organoid Tech Organoid-derived monolayers and uses thereof
CN117957310A (zh) * 2021-09-30 2024-04-30 Jsr株式会社 类器官的制造方法、类器官制造用培养基、类器官、及被检物质的评价方法
CN114181886B (zh) * 2021-11-04 2024-07-19 清华大学 三维培养系统在巴马猪和食蟹猴肠上皮类器官中的应用
WO2023132730A1 (ko) * 2022-01-10 2023-07-13 연세대학교 산학협력단 타액선 조직에서 선포 세포 유래 오가노이드의 최적 배양 방법
KR102951433B1 (ko) 2022-01-10 2026-04-10 연세대학교 산학협력단 타액선 조직에서 선포 세포 유래 오가노이드의 최적 배양 방법
GB202204266D0 (en) 2022-03-25 2022-05-11 Stichting Hubrecht Organoid Tech Methods for co-culturing genotoxic bacteria and organoids
IT202300008076A1 (it) * 2023-04-26 2024-10-26 Irccs Ospedale Policlinico San Martino Terreno di coltura per la propagazione di colture primarie di cellule epiteliali e relativo metodo di utilizzo
EP4495227A1 (en) 2023-07-18 2025-01-22 HUB Organoids IP B.V. Co-cultures of organoids and stromal cells
NL2035481B1 (en) 2023-07-26 2025-02-10 Koninklijke Nederlandse Akademie Van Wetenschappen Single-chain variable fragment of anti-integrin antibodies
WO2025085577A1 (en) * 2023-10-17 2025-04-24 NetNoids Rx Laboratories, LLC Compositions and methods for accelerating development of cancer therapeutics
CN117467616B (zh) * 2023-12-26 2024-03-26 北京基石生命科技有限公司 用于制备口腔癌微肿瘤模型的专用培养基以及相关的方法产品和应用
CN119842611A (zh) * 2025-02-06 2025-04-18 中国人民解放军总医院第六医学中心 一种造血干细胞的培养方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012000097A (ja) * 2010-06-15 2012-01-05 Tokyo Women's Medical College 組織幹細胞の増殖方法、およびそれより得られる組織幹細胞
JP2012254081A (ja) * 2009-02-03 2012-12-27 Koninkl Nederlandse Akademie Van Wetenschappen 上皮幹細胞および該幹細胞を含むオルガノイドのための培養培地
JP2014516562A (ja) * 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地
JP2018503360A (ja) * 2014-11-27 2018-02-08 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 培養培地

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
EP2328924A2 (en) * 2008-08-05 2011-06-08 Institut National De La Sante Et De La Recherche Medicale Use of agonists of integrin alpha 5 for inducing the osteogenic differentiation of mesenchymal stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
CA2818336C (en) * 2010-11-16 2018-04-24 Texas Heart Institute Agonists that enhance binding of integrin-expressing cells to integrin receptors
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
US20150140013A1 (en) * 2012-09-21 2015-05-21 The General Hospital Corporation Modulation of asymmetric proliferation
AU2015261380B2 (en) * 2014-05-16 2021-04-15 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
GB201610748D0 (en) 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012254081A (ja) * 2009-02-03 2012-12-27 Koninkl Nederlandse Akademie Van Wetenschappen 上皮幹細胞および該幹細胞を含むオルガノイドのための培養培地
JP2012000097A (ja) * 2010-06-15 2012-01-05 Tokyo Women's Medical College 組織幹細胞の増殖方法、およびそれより得られる組織幹細胞
JP2014516562A (ja) * 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地
JP2018503360A (ja) * 2014-11-27 2018-02-08 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 培養培地

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BYRON, A. ET AL.: "Anti-integrin monoclonal antibodies", JOURNAL OF CELL SCIENCE, vol. 122, JPN6024015123, 2009, pages 4009 - 4011, ISSN: 0005460153 *

Also Published As

Publication number Publication date
EP3987005A1 (en) 2022-04-27
CA3140269A1 (en) 2020-11-26
GB201906978D0 (en) 2019-07-03
US20220340879A1 (en) 2022-10-27
WO2020234250A1 (en) 2020-11-26
KR20220024075A (ko) 2022-03-03
AU2020277651A1 (en) 2022-01-20
SG11202112547SA (en) 2021-12-30
JP2025090761A (ja) 2025-06-17
CN114144516A (zh) 2022-03-04
IL288046A (en) 2022-01-01

Similar Documents

Publication Publication Date Title
JP2022532760A (ja) インテグリンアゴニストを使用する改善された培養法
US20250154472A1 (en) Culture Medium for Expanding Breast Epithelial Stem Cells
AU2015352351B2 (en) Culture medium
JP2020202867A (ja) キメラ受容体及びその使用方法
JP7285520B2 (ja) 皮膚由来多能性前駆細胞の作製方法
US20170037366A1 (en) Enrichment and characterization of human corneal endothelial cells (hcenc) with novel monoclonal antibody
JP2018502084A (ja) ベータ細胞への変換と組み合わせられたアルファ細胞の再生
CA2968751C (en) Culture medium for expanding breast epithelial stem cells
WO2025229538A1 (en) Materials, methods, and systems for cellular expansion of gamma delta t-cells
Bhati Cell line engineering and bioassay development for therapeutic monoclonal antibodies
HK40069127A (en) Culture medium for expanding breast epithelial stem cells
HK1245324B (en) Culture medium for expanding breast epithelial stem cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220307

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230509

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230509

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20230620

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20240415

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240422

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20240612

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20241114