JP2022524864A - 遺伝子サイレンシングを介した有害生物防除のための植物細胞内におけるdsRNAの生成 - Google Patents

遺伝子サイレンシングを介した有害生物防除のための植物細胞内におけるdsRNAの生成 Download PDF

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JP2022524864A
JP2022524864A JP2021555366A JP2021555366A JP2022524864A JP 2022524864 A JP2022524864 A JP 2022524864A JP 2021555366 A JP2021555366 A JP 2021555366A JP 2021555366 A JP2021555366 A JP 2021555366A JP 2022524864 A JP2022524864 A JP 2022524864A
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rna
plant
gene
mir
silencing
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JP2022524864A5 (enExample
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マオリ、エヤル
ガランティ、ヤーロン
ピグノッチ、クリスティナ
ガルシア、アンジェラ チャパッロ
メイア、オフィール
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トロピック バイオサイエンシーズ ユーケー リミテッド
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JP2021555366A 2019-03-14 2020-03-12 遺伝子サイレンシングを介した有害生物防除のための植物細胞内におけるdsRNAの生成 Pending JP2022524864A (ja)

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GB1903521.1 2019-03-14
GBGB1903521.1A GB201903521D0 (en) 2019-03-14 2019-03-14 No title
PCT/IB2020/052245 WO2020183416A1 (en) 2019-03-14 2020-03-12 PRODUCTION OF dsRNA IN PLANT CELLS FOR PEST PROTECTION VIA GENE SILENCING

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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
GB201903519D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB202103256D0 (en) * 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN113699179B (zh) * 2021-07-30 2022-04-05 广东省农业科学院植物保护研究所 osa-miR-162a在制备驱避褐飞虱绿色农药中的应用
US20250280834A1 (en) * 2021-09-15 2025-09-11 The University Of Queensland Control of insect pests
CN116064644B (zh) * 2022-10-10 2023-10-10 中国农业科学院生物技术研究所 SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用
CN115678903B (zh) * 2022-11-03 2024-04-02 贵州大学 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用
CN116144681B (zh) * 2022-12-29 2024-07-09 中国农业科学院植物保护研究所 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用
CN116082481B (zh) * 2023-03-15 2025-11-04 南京农业大学 一种影响植物对炭疽病敏感性的转录因子PbbZIP4、蛋白及其应用
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
CN116732041B (zh) * 2023-05-23 2024-06-14 贵州大学 一种可用于rna干扰防治桃蚜的致死基因、rna干扰序列及其制备方法
EP4497828A1 (en) * 2023-07-28 2025-01-29 Consejo Superior De Investigaciones Cientificas (Csic) Minimal mirna precursors
CN120283056A (zh) * 2023-10-30 2025-07-08 上海交通大学 一种靶向抑制基因表达的方法及应用
CN117587018B (zh) 2023-12-22 2024-08-27 青岛农业大学 防止dsRNA菌液被紫外降解的方法及其在烟粉虱防治中的应用
CN118726396B (zh) * 2024-07-10 2025-07-29 西部(重庆)科学城种质创制大科学中心 过表达CiKPI基因及在提高柑橘炭疽病抗性的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規dνa及びハイブリツドdνa
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスrnaベクタ−
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
DE3784860D1 (de) 1986-12-05 1993-04-22 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
ATE108828T1 (de) 1987-02-09 1994-08-15 Lubrizol Genetics Inc Hybrides rns-virus.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
CA2359868A1 (en) 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
ES2614943T3 (es) 2005-09-16 2017-06-02 Monsanto Technology Llc Procedimientos para el control genético de infestaciones de insectos en plantas y composiciones de los mismos
EP2650366B1 (en) 2005-10-18 2017-03-22 Precision Biosciences Rationally designed meganucleases with altered sequence specificity and DNA-binding affinity
WO2008148223A1 (en) 2007-06-07 2008-12-11 Agriculture And Agri-Food Canada Nanocarrier based plant transfection and transduction
EP2173901A4 (en) * 2007-06-29 2011-12-14 Boston Biomedical Inc DNA MODULATION INDUCED BY MICRO-RNA BACTERIA
BRPI0817911B8 (pt) 2007-10-05 2022-06-28 Dow Agrosciences Llc Processos para transferência de substâncias moleculares para dentro de células vegetais e processo para expressão de um gene
EP2292176B1 (en) 2009-09-07 2019-01-09 Nobel Biocare Services AG Implantation set
CA2899650A1 (en) 2012-02-29 2013-09-06 Benitec Biopharma Limited Pain treatment
JP2016507514A (ja) 2013-01-08 2016-03-10 ベニテック バイオファーマ リミテッド 加齢黄斑変性の処置
CA3221516A1 (en) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
WO2016089433A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANOOP S. SINDHU ET AL., JOURNAL OF EXPERIMENTAL BOTANY, vol. 60, no. 1, JPN6024014502, 2009, pages 315 - 324, ISSN: 0005304909 *
ELENA SENIS ET AL., NUCLEIC ACIDS RESEARCH, vol. Vol. 45, No. 1, e3, JPN6022043622, 2017, pages 1 - 17, ISSN: 0005304907 *
JIANG ZHANG ET AL., TRENDS IN BIOTECHNOLOGY, vol. 35, no. 9, JPN6024014503, 2017, pages 871 - 882, ISSN: 0005304910 *
JIANPING ZHOU ET AL., FRONTIERS IN PLANTSCIENCE, vol. 8, no. 1598, JPN6022043623, 2017, pages 1 - 12, ISSN: 0005304908 *

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