JP2022520428A - Ｒｕｖｃドメインを有する酵素 - Google Patents

Ｒｕｖｃドメインを有する酵素 Download PDF

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JP2022520428A
JP2022520428A JP2021547336A JP2021547336A JP2022520428A JP 2022520428 A JP2022520428 A JP 2022520428A JP 2021547336 A JP2021547336 A JP 2021547336A JP 2021547336 A JP2021547336 A JP 2021547336A JP 2022520428 A JP2022520428 A JP 2022520428A
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seq
sequence
endonuclease
nuclease system
engineered
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JPWO2020168291A5 (https=
JP2022520428A5 (https=
Inventor
トーマス，ブライアン
ブラウン，クリストファー
カンター，ローズ
デヴォート，オードラ
バターフィールド，クリスティーナ
アレクサンダー，リサ
エス．エー． ゴルツマン，ダニエラ
リュー，ジェイソン
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メタゲノミ アイピー テクノロジーズ，エルエルシー
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Publication of JP2022520428A publication Critical patent/JP2022520428A/ja
Publication of JPWO2020168291A5 publication Critical patent/JPWO2020168291A5/ja
Publication of JP2022520428A5 publication Critical patent/JP2022520428A5/ja
Priority to JP2023146414A priority Critical patent/JP7502537B2/ja
Priority to JP2024091759A priority patent/JP2024133476A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2021547336A 2019-02-14 2020-02-14 Ｒｕｖｃドメインを有する酵素 Ceased JP2022520428A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023146414A JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962805868P 2019-02-14 2019-02-14
US201962805878P 2019-02-14 2019-02-14
US201962805899P 2019-02-14 2019-02-14
US62/805,878 2019-02-14
US62/805,868 2019-02-14
US62/805,899 2019-02-14
US201962874414P 2019-07-15 2019-07-15
US62/874,414 2019-07-15
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023146414A Division JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素

Publications (3)

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JP2022520428A true JP2022520428A (ja) 2022-03-30
JPWO2020168291A5 JPWO2020168291A5 (https=) 2023-02-21
JP2022520428A5 JP2022520428A5 (https=) 2023-02-21

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JP2021547336A Ceased JP2022520428A (ja) 2019-02-14 2020-02-14 Ｒｕｖｃドメインを有する酵素
JP2023146414A Active JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A Pending JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

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JP2023146414A Active JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A Pending JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

Country Status (9)

Country Link
US (1) US20240117330A1 (https=)
EP (1) EP3924482A4 (https=)
JP (3) JP2022520428A (https=)
KR (2) KR102623312B1 (https=)
CN (2) CN113728098A (https=)
AU (3) AU2020223370B2 (https=)
CA (2) CA3130135C (https=)
MX (2) MX2021009886A (https=)
WO (1) WO2020168291A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023179468A (ja) * 2019-02-14 2023-12-19 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
US12410449B2 (en) 2021-11-24 2025-09-09 Metagenomi, Inc. Endonuclease systems
US12435323B2 (en) 2021-08-27 2025-10-07 Metagenomi, Inc. Enzymes with RUVC domains

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EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3823633A4 (en) 2018-06-29 2023-05-03 Editas Medicine, Inc. SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO
US10913941B2 (en) 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
GB2594339B (en) * 2020-02-14 2023-02-08 Metagenomi Inc Enzymes with RUVC domains
GB2617658B (en) 2020-03-06 2024-04-17 Metagenomi Inc Class II, type V CRISPR systems
GB2608292B (en) * 2020-03-31 2024-02-14 Metagenomi Inc Class II, type II CRISPR systems
EP4165177A4 (en) * 2020-05-08 2024-08-28 Metagenomi, Inc. ENZYMES WITH RUVC DOMAINS
KR20230021657A (ko) * 2020-05-08 2023-02-14 메타지노미, 인크. Ruvc 도메인을 포함하는 효소
CA3192224A1 (en) 2020-09-11 2022-03-17 Jyun-Liang LIN Base editing enzymes
GB2700205B (en) * 2020-09-11 2026-04-15 Metagenomi Therapeutics Inc Base editing enzymes
US20230383273A1 (en) * 2020-10-21 2023-11-30 Emendobio Inc. Novel omni 56, 58, 65, 68, 71, 75, 78, and 84 crispr nucleases
WO2022098681A2 (en) * 2020-11-03 2022-05-12 Caspr Biotech Corporation Novel class 2 crispr-cas rna-guided endonucleases
WO2022159742A1 (en) * 2021-01-22 2022-07-28 Metagenomi, Inc Novel engineered and chimeric nucleases
BR112023014719A2 (pt) * 2021-01-22 2023-12-05 Metagenomi Inc Novas nucleases quiméricas e manipuladas
BR112023018948A2 (pt) * 2021-03-19 2023-10-17 Metagenomi Inc Edição multiplex com enzimas cas
WO2022232638A2 (en) * 2021-04-30 2022-11-03 Metagenomi, Inc. Enzymes with ruvc domains
AU2022380842A1 (en) 2021-11-05 2024-05-23 Metagenomi Therapeutics, Inc. Base editing enzymes
WO2023122663A2 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2023194359A1 (en) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions and methods for treatment of usher syndrome type 2a
CN119923466A (zh) * 2022-06-15 2025-05-02 比姆医疗股份有限公司 用于减少补体激活的组合物和方法
US20260014279A1 (en) * 2022-09-16 2026-01-15 Alia Therapeutics Srl Enqp type cas proteins and applications thereof
KR20250153814A (ko) * 2023-03-01 2025-10-27 메타지노미, 인크. Ruvc 도메인을 갖는 효소
WO2025106934A1 (en) * 2023-11-16 2025-05-22 Metagenomi, Inc. Enzymes with ruvc domains

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AU2020223370B2 (en) 2019-02-14 2023-04-20 Metagenomi Therapeutics, Inc. Enzymes with RuvC domains

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023179468A (ja) * 2019-02-14 2023-12-19 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
JP7502537B2 (ja) 2019-02-14 2024-06-18 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
US12435323B2 (en) 2021-08-27 2025-10-07 Metagenomi, Inc. Enzymes with RUVC domains
US12410449B2 (en) 2021-11-24 2025-09-09 Metagenomi, Inc. Endonuclease systems

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Publication number Publication date
CA3130135C (en) 2024-11-19
KR102623312B1 (ko) 2024-01-09
EP3924482A4 (en) 2023-04-05
JP2024133476A (ja) 2024-10-02
EP3924482A1 (en) 2021-12-22
JP7502537B2 (ja) 2024-06-18
MX2023006575A (es) 2023-06-16
AU2020223370A1 (en) 2021-10-07
JP2023179468A (ja) 2023-12-19
KR20210139254A (ko) 2021-11-22
AU2025279720A1 (en) 2026-01-15
KR20240007322A (ko) 2024-01-16
WO2020168291A1 (en) 2020-08-20
MX2021009886A (es) 2021-10-13
US20240117330A1 (en) 2024-04-11
CA3130135A1 (en) 2020-08-20
AU2023206079A1 (en) 2023-08-10
CA3241703A1 (en) 2020-08-20
CN113728098A (zh) 2021-11-30
CA3241703C (en) 2026-02-24
AU2020223370B2 (en) 2023-04-20
CN116515797A (zh) 2023-08-01

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