JP2022520428A - Ｒｕｖｃドメインを有する酵素 - Google Patents

Ｒｕｖｃドメインを有する酵素 Download PDF

Info

Publication number
JP2022520428A
JP2022520428A JP2021547336A JP2021547336A JP2022520428A JP 2022520428 A JP2022520428 A JP 2022520428A JP 2021547336 A JP2021547336 A JP 2021547336A JP 2021547336 A JP2021547336 A JP 2021547336A JP 2022520428 A JP2022520428 A JP 2022520428A
Authority
JP
Japan
Prior art keywords
seq
sequence
endonuclease
nuclease system
engineered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2021547336A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022520428A5 (enExample
Inventor
トーマス，ブライアン
ブラウン，クリストファー
カンター，ローズ
デヴォート，オードラ
バターフィールド，クリスティーナ
アレクサンダー，リサ
エス．エー． ゴルツマン，ダニエラ
リュー，ジェイソン
Original Assignee
メタゲノミ アイピー テクノロジーズ，エルエルシー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by メタゲノミ アイピー テクノロジーズ，エルエルシー filed Critical メタゲノミ アイピー テクノロジーズ，エルエルシー
Publication of JP2022520428A publication Critical patent/JP2022520428A/ja
Publication of JP2022520428A5 publication Critical patent/JP2022520428A5/ja
Priority to JP2023146414A priority Critical patent/JP7502537B2/ja
Priority to JP2024091759A priority patent/JP2024133476A/ja
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2021547336A 2019-02-14 2020-02-14 Ｒｕｖｃドメインを有する酵素 Ceased JP2022520428A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023146414A JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962805878P 2019-02-14 2019-02-14
US201962805899P 2019-02-14 2019-02-14
US201962805868P 2019-02-14 2019-02-14
US62/805,868 2019-02-14
US62/805,899 2019-02-14
US62/805,878 2019-02-14
US201962874414P 2019-07-15 2019-07-15
US62/874,414 2019-07-15
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023146414A Division JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素

Publications (2)

Publication Number Publication Date
JP2022520428A true JP2022520428A (ja) 2022-03-30
JP2022520428A5 JP2022520428A5 (enExample) 2023-02-21

Family

ID=72045639

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2021547336A Ceased JP2022520428A (ja) 2019-02-14 2020-02-14 Ｒｕｖｃドメインを有する酵素
JP2023146414A Active JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A Pending JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2023146414A Active JP7502537B2 (ja) 2019-02-14 2023-09-08 Ｒｕｖｃドメインを有する酵素
JP2024091759A Pending JP2024133476A (ja) 2019-02-14 2024-06-05 Ｒｕｖｃドメインを有する酵素

Country Status (9)

Country Link
US (1) US20240117330A1 (enExample)
EP (1) EP3924482A4 (enExample)
JP (3) JP2022520428A (enExample)
KR (2) KR20240007322A (enExample)
CN (2) CN116515797A (enExample)
AU (3) AU2020223370B2 (enExample)
CA (2) CA3241703C (enExample)
MX (2) MX2021009886A (enExample)
WO (1) WO2020168291A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023179468A (ja) * 2019-02-14 2023-12-19 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
US12410449B2 (en) 2021-11-24 2025-09-09 Metagenomi, Inc. Endonuclease systems
US12435323B2 (en) 2021-08-27 2025-10-07 Metagenomi, Inc. Enzymes with RUVC domains
US12692487B2 (en) 2019-02-14 2026-07-28 Metagenomi Therapeutics, Inc. Enzymes with RuvC domains

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021178933A2 (en) 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
AU2019291918B2 (en) 2018-06-29 2025-06-12 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
GB2617659B (en) * 2020-02-14 2024-05-01 Metagenomi Inc Enzymes with RuvC domains
CA3177051A1 (en) * 2020-03-31 2021-10-07 Metagenomi, Inc. Class ii, type ii crispr systems
CN116096892A (zh) * 2020-05-08 2023-05-09 宏基因组学公司 具有RuvC结构域的酶
WO2021226369A1 (en) * 2020-05-08 2021-11-11 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains
CA3192224A1 (en) 2020-09-11 2022-03-17 Jyun-Liang LIN Base editing enzymes
GB202418708D0 (en) * 2020-09-11 2025-02-05 Metagenomi Inc Base editing enzymes
JP2023546694A (ja) * 2020-10-21 2023-11-07 エメンドバイオ・インコーポレイテッド 新規のｏｍｎｉ５６、５８、６５、６８、７１、７５、７８及び８４ｃｒｉｓｐｒヌクレアーゼ
EP4240853A2 (en) * 2020-11-03 2023-09-13 Science Solutions LLC Novel class 2 crispr-cas rna-guided endonucleases
CN116964197A (zh) * 2021-01-22 2023-10-27 宏基因组学公司 新型工程化和嵌合核酸酶
WO2022159742A1 (en) * 2021-01-22 2022-07-28 Metagenomi, Inc Novel engineered and chimeric nucleases
KR20230134543A (ko) * 2021-01-22 2023-09-21 메타지노미, 인크. 신규한 조작된 뉴클레아제 및 키메라 뉴클레아제
JP7877351B2 (ja) * 2021-03-19 2026-06-22 メタゲノミ セラピューティクス，インク． Ｃａｓ酵素を用いたマルチプレックス編集
EP4330386A4 (en) * 2021-04-30 2025-05-14 Metagenomi, Inc. Enzymes with ruvc domains
MX2024005505A (es) 2021-11-05 2024-05-23 Metagenomi Inc Enzimas de edicion de bases.
WO2023122663A2 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2023194359A1 (en) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions and methods for treatment of usher syndrome type 2a
KR20250037788A (ko) * 2022-06-15 2025-03-18 빔 테라퓨틱스, 인크. 보체 활성화를 감소시키기 위한 조성물 및 방법
EP4587564A2 (en) * 2022-09-16 2025-07-23 Alia Therapeutics Srl Enqp type ii cas proteins and applications thereof
AU2024230342A1 (en) * 2023-03-01 2025-10-16 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
WO2025106934A1 (en) * 2023-11-16 2025-05-22 Metagenomi, Inc. Enzymes with ruvc domains

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209621B (zh) * 2012-12-12 2021-05-25 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
US10392607B2 (en) * 2015-06-03 2019-08-27 The Regents Of The University Of California Cas9 variants and methods of use thereof
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
WO2018035250A1 (en) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
KR20200124702A (ko) * 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
KR20240007322A (ko) 2019-02-14 2024-01-16 메타지노미, 인크. Ruvc 도메인이 존재하는 효소

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"ID A0A1F0PN46_9MICC", UNIPROT, JPN6023010401, 13 February 2019 (2019-02-13), ISSN: 0005013894 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023179468A (ja) * 2019-02-14 2023-12-19 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
JP7502537B2 (ja) 2019-02-14 2024-06-18 メタゲノミ，インク． Ｒｕｖｃドメインを有する酵素
US12692487B2 (en) 2019-02-14 2026-07-28 Metagenomi Therapeutics, Inc. Enzymes with RuvC domains
US12435323B2 (en) 2021-08-27 2025-10-07 Metagenomi, Inc. Enzymes with RUVC domains
US12410449B2 (en) 2021-11-24 2025-09-09 Metagenomi, Inc. Endonuclease systems

Also Published As

Publication number Publication date
CN113728098A (zh) 2021-11-30
KR20240007322A (ko) 2024-01-16
MX2021009886A (es) 2021-10-13
CA3241703A1 (en) 2020-08-20
KR20210139254A (ko) 2021-11-22
AU2020223370A1 (en) 2021-10-07
JP2024133476A (ja) 2024-10-02
CA3130135C (en) 2024-11-19
CA3241703C (en) 2026-02-24
CN116515797A (zh) 2023-08-01
EP3924482A4 (en) 2023-04-05
AU2025279720A1 (en) 2026-01-15
WO2020168291A1 (en) 2020-08-20
AU2023206079A1 (en) 2023-08-10
AU2020223370B2 (en) 2023-04-20
JP7502537B2 (ja) 2024-06-18
CA3130135A1 (en) 2020-08-20
KR102623312B1 (ko) 2024-01-09
EP3924482A1 (en) 2021-12-22
MX2023006575A (es) 2023-06-16
US20240117330A1 (en) 2024-04-11
JP2023179468A (ja) 2023-12-19

Similar Documents

Publication Publication Date Title
JP7502537B2 (ja) Ｒｕｖｃドメインを有する酵素
US20240344045A1 (en) Enzymes with ruvc domains
US20240209332A1 (en) Enzymes with ruvc domains
US20220298494A1 (en) Enzymes with ruvc domains
US20240110167A1 (en) Enzymes with ruvc domains
US20220220460A1 (en) Enzymes with ruvc domains
WO2021226369A1 (en) Enzymes with ruvc domains
CA3225082A1 (en) Enzymes with ruvc domains
US20240301374A1 (en) Systems and methods for transposing cargo nucleotide sequences
GB2617659A (en) Enzymes with RUVC domains
HK40065084A (en) Enzymes with ruvc domains
HK40120300A (en) Enzymes with ruvc domains

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211021

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220404

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A712

Effective date: 20220511

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20220511

RD12 Notification of acceptance of power of sub attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7432

Effective date: 20221221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20221221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230210

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230210

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20230217

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230315

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230615

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230814

A045 Written measure of dismissal of application [lapsed due to lack of payment]

Free format text: JAPANESE INTERMEDIATE CODE: A045

Effective date: 20231218